anti nachr α 7  (Alomone Labs)


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    Alomone Labs anti nachr α 7
    The proposed model of an immunomodulatory mechanism utilized by human MSCs in an inflammatory milieu. The inflammatory conditions drive human MSCs to adopt a neuronlike phenotype. It is probable that the expression of neurotrophins such as NGF and BDNF in human AICs and the presence of NRs on MSCs are associated with these changes in MSCs. Furthermore, the inflammatory milieu probably induces the expression of <t>nAChR</t> including nAChR α 7, which participates in the negative regulation of activated lymphocytes. Neuronlike MSCs stimulated by neurotrophins and unknown factors secrete ACh, which binds to AICs via nAChR α 7, thereby inhibiting the proliferation and function of AICs.
    Anti Nachr α 7, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nachr α 7/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti nachr α 7 - by Bioz Stars, 2022-01
    94/100 stars

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    1) Product Images from "A Novel Immunomodulatory Mechanism Dependent on Acetylcholine Secreted by Human Bone Marrow-derived Mesenchymal Stem Cells"

    Article Title: A Novel Immunomodulatory Mechanism Dependent on Acetylcholine Secreted by Human Bone Marrow-derived Mesenchymal Stem Cells

    Journal: International Journal of Stem Cells

    doi: 10.15283/ijsc18098

    The proposed model of an immunomodulatory mechanism utilized by human MSCs in an inflammatory milieu. The inflammatory conditions drive human MSCs to adopt a neuronlike phenotype. It is probable that the expression of neurotrophins such as NGF and BDNF in human AICs and the presence of NRs on MSCs are associated with these changes in MSCs. Furthermore, the inflammatory milieu probably induces the expression of nAChR including nAChR α 7, which participates in the negative regulation of activated lymphocytes. Neuronlike MSCs stimulated by neurotrophins and unknown factors secrete ACh, which binds to AICs via nAChR α 7, thereby inhibiting the proliferation and function of AICs.
    Figure Legend Snippet: The proposed model of an immunomodulatory mechanism utilized by human MSCs in an inflammatory milieu. The inflammatory conditions drive human MSCs to adopt a neuronlike phenotype. It is probable that the expression of neurotrophins such as NGF and BDNF in human AICs and the presence of NRs on MSCs are associated with these changes in MSCs. Furthermore, the inflammatory milieu probably induces the expression of nAChR including nAChR α 7, which participates in the negative regulation of activated lymphocytes. Neuronlike MSCs stimulated by neurotrophins and unknown factors secrete ACh, which binds to AICs via nAChR α 7, thereby inhibiting the proliferation and function of AICs.

    Techniques Used: Expressing

    Inflammatory conditions induce a cholinergic-neuron–like phenotype in MSCs and nAChRs in activated PBMCs. (a) Immunofluorescence staining of ChAT, GABA, and TH in MSCs cocultured with activated PBMCs (48 h). (b) qPCR was carried out to quantify ChAT expression in MSCs after inflammatory stimulation for 24 h. (c) Western blotting confirmed ChAT expression in the whole MSC extract (20 μ g) after coculture for 48 h. (d) ACh and choline concentration was measured in the CM obtained from PBMCs alone (P or P o ), PHA-activated PBMCs (P PHA ), or MLR (P and P o ) culture without or with MSCs (n=3). (e) ACh and choline concentration was measured in the CM obtained from PBMCs alone (P or P o ) or MLR (P and P o ) culture without or with MSCs for 48 h. “MLR sup.” is the supernatant from the MLR without MSCs. “(MLR+MSC) sup.” is the supernatant from MLR with MSCs (n=3). (f) RT-PCR analysis of several nAChR subunits in activated PBMCs. (g) qPCR was carried out to assess nAChR α5 expression in activated PBMCs after incubation for 24 h. (h) qPCR was performed to measure nAChR α7 expression in activated PBMCs after incubation for 24 h. (i) An increase in the protein expression of the nAChR α 7 subunit in activated PBMCs was confirmed by western blotting of whole MSC extracts prepared after MLR or PHA stimulation for 48 h.
    Figure Legend Snippet: Inflammatory conditions induce a cholinergic-neuron–like phenotype in MSCs and nAChRs in activated PBMCs. (a) Immunofluorescence staining of ChAT, GABA, and TH in MSCs cocultured with activated PBMCs (48 h). (b) qPCR was carried out to quantify ChAT expression in MSCs after inflammatory stimulation for 24 h. (c) Western blotting confirmed ChAT expression in the whole MSC extract (20 μ g) after coculture for 48 h. (d) ACh and choline concentration was measured in the CM obtained from PBMCs alone (P or P o ), PHA-activated PBMCs (P PHA ), or MLR (P and P o ) culture without or with MSCs (n=3). (e) ACh and choline concentration was measured in the CM obtained from PBMCs alone (P or P o ) or MLR (P and P o ) culture without or with MSCs for 48 h. “MLR sup.” is the supernatant from the MLR without MSCs. “(MLR+MSC) sup.” is the supernatant from MLR with MSCs (n=3). (f) RT-PCR analysis of several nAChR subunits in activated PBMCs. (g) qPCR was carried out to assess nAChR α5 expression in activated PBMCs after incubation for 24 h. (h) qPCR was performed to measure nAChR α7 expression in activated PBMCs after incubation for 24 h. (i) An increase in the protein expression of the nAChR α 7 subunit in activated PBMCs was confirmed by western blotting of whole MSC extracts prepared after MLR or PHA stimulation for 48 h.

    Techniques Used: Immunofluorescence, Staining, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Concentration Assay, Reverse Transcription Polymerase Chain Reaction, Incubation

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    Alomone Labs anti nachr α 7
    The proposed model of an immunomodulatory mechanism utilized by human MSCs in an inflammatory milieu. The inflammatory conditions drive human MSCs to adopt a neuronlike phenotype. It is probable that the expression of neurotrophins such as NGF and BDNF in human AICs and the presence of NRs on MSCs are associated with these changes in MSCs. Furthermore, the inflammatory milieu probably induces the expression of <t>nAChR</t> including nAChR α 7, which participates in the negative regulation of activated lymphocytes. Neuronlike MSCs stimulated by neurotrophins and unknown factors secrete ACh, which binds to AICs via nAChR α 7, thereby inhibiting the proliferation and function of AICs.
    Anti Nachr α 7, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nachr α 7/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti nachr α 7 - by Bioz Stars, 2022-01
    94/100 stars
      Buy from Supplier

    96
    Alomone Labs α7 nachr
    A role for lipid rafts in ACE2 and <t>α7</t> <t>nAChR</t> association. a) Western blot detection of ACE2 protein expression in cells. b) Colocalization of ACE2 and cell surface α7 nAChRs (fBgtx). c) A Pearson’s correlation coefficient (PCC) of ACE2 and α7 nAChR cellular colocalization with statistical analysis calculated using a one-way ANOVA, followed by Tukey’s HSD post-hoc analysis (F(3,58)=4.194). Scale bar = 5 μm.
    α7 Nachr, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α7 nachr/product/Alomone Labs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α7 nachr - by Bioz Stars, 2022-01
    96/100 stars
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    The proposed model of an immunomodulatory mechanism utilized by human MSCs in an inflammatory milieu. The inflammatory conditions drive human MSCs to adopt a neuronlike phenotype. It is probable that the expression of neurotrophins such as NGF and BDNF in human AICs and the presence of NRs on MSCs are associated with these changes in MSCs. Furthermore, the inflammatory milieu probably induces the expression of nAChR including nAChR α 7, which participates in the negative regulation of activated lymphocytes. Neuronlike MSCs stimulated by neurotrophins and unknown factors secrete ACh, which binds to AICs via nAChR α 7, thereby inhibiting the proliferation and function of AICs.

    Journal: International Journal of Stem Cells

    Article Title: A Novel Immunomodulatory Mechanism Dependent on Acetylcholine Secreted by Human Bone Marrow-derived Mesenchymal Stem Cells

    doi: 10.15283/ijsc18098

    Figure Lengend Snippet: The proposed model of an immunomodulatory mechanism utilized by human MSCs in an inflammatory milieu. The inflammatory conditions drive human MSCs to adopt a neuronlike phenotype. It is probable that the expression of neurotrophins such as NGF and BDNF in human AICs and the presence of NRs on MSCs are associated with these changes in MSCs. Furthermore, the inflammatory milieu probably induces the expression of nAChR including nAChR α 7, which participates in the negative regulation of activated lymphocytes. Neuronlike MSCs stimulated by neurotrophins and unknown factors secrete ACh, which binds to AICs via nAChR α 7, thereby inhibiting the proliferation and function of AICs.

    Article Snippet: The following antibodies were employed for immunodetection: anti-TrkA (cat. # 06-574; EMD Millipore), anti-TrkC (cat. # 3376; Cell Signaling Technology, Danvers, MA, USA), anti-p75NTR (cat. # 8238; Cell Signaling Technology), anti-ChAT (cat. # AB144P; EMD Millipore), anti–nAChR α 7 (cat. # ANC-007; Alomone, Jerusalem, Israel), and anti–β -actin (cat. # SC-47778; Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Expressing

    Inflammatory conditions induce a cholinergic-neuron–like phenotype in MSCs and nAChRs in activated PBMCs. (a) Immunofluorescence staining of ChAT, GABA, and TH in MSCs cocultured with activated PBMCs (48 h). (b) qPCR was carried out to quantify ChAT expression in MSCs after inflammatory stimulation for 24 h. (c) Western blotting confirmed ChAT expression in the whole MSC extract (20 μ g) after coculture for 48 h. (d) ACh and choline concentration was measured in the CM obtained from PBMCs alone (P or P o ), PHA-activated PBMCs (P PHA ), or MLR (P and P o ) culture without or with MSCs (n=3). (e) ACh and choline concentration was measured in the CM obtained from PBMCs alone (P or P o ) or MLR (P and P o ) culture without or with MSCs for 48 h. “MLR sup.” is the supernatant from the MLR without MSCs. “(MLR+MSC) sup.” is the supernatant from MLR with MSCs (n=3). (f) RT-PCR analysis of several nAChR subunits in activated PBMCs. (g) qPCR was carried out to assess nAChR α5 expression in activated PBMCs after incubation for 24 h. (h) qPCR was performed to measure nAChR α7 expression in activated PBMCs after incubation for 24 h. (i) An increase in the protein expression of the nAChR α 7 subunit in activated PBMCs was confirmed by western blotting of whole MSC extracts prepared after MLR or PHA stimulation for 48 h.

    Journal: International Journal of Stem Cells

    Article Title: A Novel Immunomodulatory Mechanism Dependent on Acetylcholine Secreted by Human Bone Marrow-derived Mesenchymal Stem Cells

    doi: 10.15283/ijsc18098

    Figure Lengend Snippet: Inflammatory conditions induce a cholinergic-neuron–like phenotype in MSCs and nAChRs in activated PBMCs. (a) Immunofluorescence staining of ChAT, GABA, and TH in MSCs cocultured with activated PBMCs (48 h). (b) qPCR was carried out to quantify ChAT expression in MSCs after inflammatory stimulation for 24 h. (c) Western blotting confirmed ChAT expression in the whole MSC extract (20 μ g) after coculture for 48 h. (d) ACh and choline concentration was measured in the CM obtained from PBMCs alone (P or P o ), PHA-activated PBMCs (P PHA ), or MLR (P and P o ) culture without or with MSCs (n=3). (e) ACh and choline concentration was measured in the CM obtained from PBMCs alone (P or P o ) or MLR (P and P o ) culture without or with MSCs for 48 h. “MLR sup.” is the supernatant from the MLR without MSCs. “(MLR+MSC) sup.” is the supernatant from MLR with MSCs (n=3). (f) RT-PCR analysis of several nAChR subunits in activated PBMCs. (g) qPCR was carried out to assess nAChR α5 expression in activated PBMCs after incubation for 24 h. (h) qPCR was performed to measure nAChR α7 expression in activated PBMCs after incubation for 24 h. (i) An increase in the protein expression of the nAChR α 7 subunit in activated PBMCs was confirmed by western blotting of whole MSC extracts prepared after MLR or PHA stimulation for 48 h.

    Article Snippet: The following antibodies were employed for immunodetection: anti-TrkA (cat. # 06-574; EMD Millipore), anti-TrkC (cat. # 3376; Cell Signaling Technology, Danvers, MA, USA), anti-p75NTR (cat. # 8238; Cell Signaling Technology), anti-ChAT (cat. # AB144P; EMD Millipore), anti–nAChR α 7 (cat. # ANC-007; Alomone, Jerusalem, Israel), and anti–β -actin (cat. # SC-47778; Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Immunofluorescence, Staining, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Concentration Assay, Reverse Transcription Polymerase Chain Reaction, Incubation

    A role for lipid rafts in ACE2 and α7 nAChR association. a) Western blot detection of ACE2 protein expression in cells. b) Colocalization of ACE2 and cell surface α7 nAChRs (fBgtx). c) A Pearson’s correlation coefficient (PCC) of ACE2 and α7 nAChR cellular colocalization with statistical analysis calculated using a one-way ANOVA, followed by Tukey’s HSD post-hoc analysis (F(3,58)=4.194). Scale bar = 5 μm.

    Journal: bioRxiv

    Article Title: Mechanisms of Coupling between Angiotensin Converting Enzyme 2 and Nicotinic Acetylcholine Receptors

    doi: 10.1101/2021.08.12.456154

    Figure Lengend Snippet: A role for lipid rafts in ACE2 and α7 nAChR association. a) Western blot detection of ACE2 protein expression in cells. b) Colocalization of ACE2 and cell surface α7 nAChRs (fBgtx). c) A Pearson’s correlation coefficient (PCC) of ACE2 and α7 nAChR cellular colocalization with statistical analysis calculated using a one-way ANOVA, followed by Tukey’s HSD post-hoc analysis (F(3,58)=4.194). Scale bar = 5 μm.

    Article Snippet: Co-immunoprecipitation (co-IP) of the α7 nAChR and its interacting proteins was performed as described in [ ], with minor modifications.

    Techniques: Western Blot, Expressing, Periodic Counter-current Chromatography

    α7 nAChR interacts with ACE2 and regulate its expression. a) Co-IP from PC12 cell membrane fractions using an anti-α7 antibody. Western blot detection using an anti-ACE2 antibody shows immunoreactive bands on the gel. b) Western blot detection of the ACE2 protein on the gel. c) Colocalization of ACE2 and the α7 nAChR (fBgtx) using a heat map to show relative change in the protein colocalization in the cell. Scale bar = 10 μm.

    Journal: bioRxiv

    Article Title: Mechanisms of Coupling between Angiotensin Converting Enzyme 2 and Nicotinic Acetylcholine Receptors

    doi: 10.1101/2021.08.12.456154

    Figure Lengend Snippet: α7 nAChR interacts with ACE2 and regulate its expression. a) Co-IP from PC12 cell membrane fractions using an anti-α7 antibody. Western blot detection using an anti-ACE2 antibody shows immunoreactive bands on the gel. b) Western blot detection of the ACE2 protein on the gel. c) Colocalization of ACE2 and the α7 nAChR (fBgtx) using a heat map to show relative change in the protein colocalization in the cell. Scale bar = 10 μm.

    Article Snippet: Co-immunoprecipitation (co-IP) of the α7 nAChR and its interacting proteins was performed as described in [ ], with minor modifications.

    Techniques: Expressing, Co-Immunoprecipitation Assay, Western Blot

    Proposed mechanisms for ACE2 and α7 nAChR coupling in cells. a) A STRING database interaction network for α7 nAChR (CHRNA7) and ACE2 shows common pathways (circled in red). b) A cell signaling model for ACE2 and α7 nAChR interaction.

    Journal: bioRxiv

    Article Title: Mechanisms of Coupling between Angiotensin Converting Enzyme 2 and Nicotinic Acetylcholine Receptors

    doi: 10.1101/2021.08.12.456154

    Figure Lengend Snippet: Proposed mechanisms for ACE2 and α7 nAChR coupling in cells. a) A STRING database interaction network for α7 nAChR (CHRNA7) and ACE2 shows common pathways (circled in red). b) A cell signaling model for ACE2 and α7 nAChR interaction.

    Article Snippet: Co-immunoprecipitation (co-IP) of the α7 nAChR and its interacting proteins was performed as described in [ ], with minor modifications.

    Techniques: