α7 nachr  (Alomone Labs)


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  • 94
    Name:
    Nicotinic Acetylcholine Receptor α7 CHRNA7 extracellular Blocking Peptide
    Description:
    A Blocking Peptide for Anti Nicotinic Acetylcholine Receptor alpha7 CHRNA7 extracellular Antibody
    Catalog Number:
    BLP-NC007
    Price:
    245.0
    Category:
    Control reagent Negative Control
    Source:
    Synthetic peptide
    Applications:
    Blocking
    Purity:
    >70% (amino acid analysis and mass spectrometry).
    Size:
    40 mcg
    Format:
    Lyophilized powder
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    Structured Review

    Alomone Labs α7 nachr
    Nicotinic Acetylcholine Receptor α7 CHRNA7 extracellular Blocking Peptide
    A Blocking Peptide for Anti Nicotinic Acetylcholine Receptor alpha7 CHRNA7 extracellular Antibody
    https://www.bioz.com/result/α7 nachr/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α7 nachr - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Mechanisms of Coupling between Angiotensin Converting Enzyme 2 and Nicotinic Acetylcholine Receptors"

    Article Title: Mechanisms of Coupling between Angiotensin Converting Enzyme 2 and Nicotinic Acetylcholine Receptors

    Journal: bioRxiv

    doi: 10.1101/2021.08.12.456154

    A role for lipid rafts in ACE2 and α7 nAChR association. a) Western blot detection of ACE2 protein expression in cells. b) Colocalization of ACE2 and cell surface α7 nAChRs (fBgtx). c) A Pearson’s correlation coefficient (PCC) of ACE2 and α7 nAChR cellular colocalization with statistical analysis calculated using a one-way ANOVA, followed by Tukey’s HSD post-hoc analysis (F(3,58)=4.194). Scale bar = 5 μm.
    Figure Legend Snippet: A role for lipid rafts in ACE2 and α7 nAChR association. a) Western blot detection of ACE2 protein expression in cells. b) Colocalization of ACE2 and cell surface α7 nAChRs (fBgtx). c) A Pearson’s correlation coefficient (PCC) of ACE2 and α7 nAChR cellular colocalization with statistical analysis calculated using a one-way ANOVA, followed by Tukey’s HSD post-hoc analysis (F(3,58)=4.194). Scale bar = 5 μm.

    Techniques Used: Western Blot, Expressing, Periodic Counter-current Chromatography

    α7 nAChR interacts with ACE2 and regulate its expression. a) Co-IP from PC12 cell membrane fractions using an anti-α7 antibody. Western blot detection using an anti-ACE2 antibody shows immunoreactive bands on the gel. b) Western blot detection of the ACE2 protein on the gel. c) Colocalization of ACE2 and the α7 nAChR (fBgtx) using a heat map to show relative change in the protein colocalization in the cell. Scale bar = 10 μm.
    Figure Legend Snippet: α7 nAChR interacts with ACE2 and regulate its expression. a) Co-IP from PC12 cell membrane fractions using an anti-α7 antibody. Western blot detection using an anti-ACE2 antibody shows immunoreactive bands on the gel. b) Western blot detection of the ACE2 protein on the gel. c) Colocalization of ACE2 and the α7 nAChR (fBgtx) using a heat map to show relative change in the protein colocalization in the cell. Scale bar = 10 μm.

    Techniques Used: Expressing, Co-Immunoprecipitation Assay, Western Blot

    Proposed mechanisms for ACE2 and α7 nAChR coupling in cells. a) A STRING database interaction network for α7 nAChR (CHRNA7) and ACE2 shows common pathways (circled in red). b) A cell signaling model for ACE2 and α7 nAChR interaction.
    Figure Legend Snippet: Proposed mechanisms for ACE2 and α7 nAChR coupling in cells. a) A STRING database interaction network for α7 nAChR (CHRNA7) and ACE2 shows common pathways (circled in red). b) A cell signaling model for ACE2 and α7 nAChR interaction.

    Techniques Used:

    Related Articles

    Incubation:

    Article Title: Mesencephalic Electrical Stimulation Reduces Neuroinflammation after Photothrombotic Stroke in Rats by Targeting the Cholinergic Anti-Inflammatory Pathway
    Article Snippet: .. In order to visualize alpha7 nicotinic acetylcholine receptor (α7nAchR), brain slices were incubated using anti-rat-nAChR α7 (CHRNA7; Alomone Labs, Jerusalem/Israel, catalogue #ANC-007), and nuclei of cells were stained using 4,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, St Louis, MO, USA, catalogue #D9542). ..

    Article Title: Paeoniflorin has anti-inflammation and neurogenesis functions through nicotinic acetylcholine receptors in cerebral ischemia-reperfusion injury rats
    Article Snippet: .. The sections were incubated with a primary antibody, CD68 (1:200) (Millipore, Washington, DC, USA) and nAChR α7 (1:200) (Alomone, Jerusalem, Israel) at 4 °C overnight. ..

    Article Title: Street RABV Induces the Cholinergic Anti-inflammatory Pathway in Human Monocyte-Derived Macrophages by Binding to nAChr α7
    Article Snippet: .. In parallel, macrophages were incubated with ATTO-633 conjugated alpha-bungarotoxin (α-BTX; Alomone labs) or with an antibody against the nicotinic acetylcholine receptor α7 (nAChr α7; Alomone Labs) followed by an Alexa594 goat-anti-rabbit conjugate (Invitrogen). ..

    Staining:

    Article Title: Mesencephalic Electrical Stimulation Reduces Neuroinflammation after Photothrombotic Stroke in Rats by Targeting the Cholinergic Anti-Inflammatory Pathway
    Article Snippet: .. In order to visualize alpha7 nicotinic acetylcholine receptor (α7nAchR), brain slices were incubated using anti-rat-nAChR α7 (CHRNA7; Alomone Labs, Jerusalem/Israel, catalogue #ANC-007), and nuclei of cells were stained using 4,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, St Louis, MO, USA, catalogue #D9542). ..

    Co-Immunoprecipitation Assay:

    Article Title: Mechanisms of Coupling between Angiotensin Converting Enzyme 2 and Nicotinic Acetylcholine Receptors
    Article Snippet: .. Co-immunoprecipitation (co-IP) of the α7 nAChR and its interacting proteins was performed as described in [ ], with minor modifications. ..

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  • News
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  • 94
    Alomone Labs α7 nachr
    A role for lipid rafts in ACE2 and <t>α7</t> <t>nAChR</t> association. a) Western blot detection of ACE2 protein expression in cells. b) Colocalization of ACE2 and cell surface α7 nAChRs (fBgtx). c) A Pearson’s correlation coefficient (PCC) of ACE2 and α7 nAChR cellular colocalization with statistical analysis calculated using a one-way ANOVA, followed by Tukey’s HSD post-hoc analysis (F(3,58)=4.194). Scale bar = 5 μm.
    α7 Nachr, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α7 nachr/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α7 nachr - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    A role for lipid rafts in ACE2 and α7 nAChR association. a) Western blot detection of ACE2 protein expression in cells. b) Colocalization of ACE2 and cell surface α7 nAChRs (fBgtx). c) A Pearson’s correlation coefficient (PCC) of ACE2 and α7 nAChR cellular colocalization with statistical analysis calculated using a one-way ANOVA, followed by Tukey’s HSD post-hoc analysis (F(3,58)=4.194). Scale bar = 5 μm.

    Journal: bioRxiv

    Article Title: Mechanisms of Coupling between Angiotensin Converting Enzyme 2 and Nicotinic Acetylcholine Receptors

    doi: 10.1101/2021.08.12.456154

    Figure Lengend Snippet: A role for lipid rafts in ACE2 and α7 nAChR association. a) Western blot detection of ACE2 protein expression in cells. b) Colocalization of ACE2 and cell surface α7 nAChRs (fBgtx). c) A Pearson’s correlation coefficient (PCC) of ACE2 and α7 nAChR cellular colocalization with statistical analysis calculated using a one-way ANOVA, followed by Tukey’s HSD post-hoc analysis (F(3,58)=4.194). Scale bar = 5 μm.

    Article Snippet: Co-immunoprecipitation (co-IP) of the α7 nAChR and its interacting proteins was performed as described in [ ], with minor modifications.

    Techniques: Western Blot, Expressing, Periodic Counter-current Chromatography

    α7 nAChR interacts with ACE2 and regulate its expression. a) Co-IP from PC12 cell membrane fractions using an anti-α7 antibody. Western blot detection using an anti-ACE2 antibody shows immunoreactive bands on the gel. b) Western blot detection of the ACE2 protein on the gel. c) Colocalization of ACE2 and the α7 nAChR (fBgtx) using a heat map to show relative change in the protein colocalization in the cell. Scale bar = 10 μm.

    Journal: bioRxiv

    Article Title: Mechanisms of Coupling between Angiotensin Converting Enzyme 2 and Nicotinic Acetylcholine Receptors

    doi: 10.1101/2021.08.12.456154

    Figure Lengend Snippet: α7 nAChR interacts with ACE2 and regulate its expression. a) Co-IP from PC12 cell membrane fractions using an anti-α7 antibody. Western blot detection using an anti-ACE2 antibody shows immunoreactive bands on the gel. b) Western blot detection of the ACE2 protein on the gel. c) Colocalization of ACE2 and the α7 nAChR (fBgtx) using a heat map to show relative change in the protein colocalization in the cell. Scale bar = 10 μm.

    Article Snippet: Co-immunoprecipitation (co-IP) of the α7 nAChR and its interacting proteins was performed as described in [ ], with minor modifications.

    Techniques: Expressing, Co-Immunoprecipitation Assay, Western Blot

    Proposed mechanisms for ACE2 and α7 nAChR coupling in cells. a) A STRING database interaction network for α7 nAChR (CHRNA7) and ACE2 shows common pathways (circled in red). b) A cell signaling model for ACE2 and α7 nAChR interaction.

    Journal: bioRxiv

    Article Title: Mechanisms of Coupling between Angiotensin Converting Enzyme 2 and Nicotinic Acetylcholine Receptors

    doi: 10.1101/2021.08.12.456154

    Figure Lengend Snippet: Proposed mechanisms for ACE2 and α7 nAChR coupling in cells. a) A STRING database interaction network for α7 nAChR (CHRNA7) and ACE2 shows common pathways (circled in red). b) A cell signaling model for ACE2 and α7 nAChR interaction.

    Article Snippet: Co-immunoprecipitation (co-IP) of the α7 nAChR and its interacting proteins was performed as described in [ ], with minor modifications.

    Techniques: