anti nicotinic ach receptor α2  (Alomone Labs)


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    Alomone Labs anti nicotinic ach receptor α2
    Localization of nAChR subunits in the mouse small intestine and organoids. ( A ) RT-PCR analysis of the expression of nAChR subunits in intestine and cultured organoids (passage 5); ( B , D ) visualization of <t>α2</t> (red) in crypts and villus; ( E , F ) control sections labeled with secondary antibody [Alexa Fluor 546 donkey anti-(rabbit IgG)] in the absence of primary antibody; ( G , I ) visualization of β4 (red) in crypts and villus; ( J , K ) control sections labeled with secondary antibody [Alexa Fluor 568 rabbit anti-(goat IgG)] in the absence of primary antibody; ( L – O ) co-localization of α2 and β4 in crypts; ( M ) visualization of α2 (green) in crypts; ( N ) visualization of β4 (red) in crypts; ( O ) merged visualization of ( L ), ( M ), and ( N ). ( C , H , P ) Enlargement of ( B ), ( G ), and ( O ). White dotted lines indicate the crypt region. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( B – P ) except for ( C ), ( H ), and ( P ) represent 20 μm.
    Anti Nicotinic Ach Receptor α2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nicotinic ach receptor α2/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti nicotinic ach receptor α2 - by Bioz Stars, 2022-01
    91/100 stars

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    1) Product Images from "The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice"

    Article Title: The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19030738

    Localization of nAChR subunits in the mouse small intestine and organoids. ( A ) RT-PCR analysis of the expression of nAChR subunits in intestine and cultured organoids (passage 5); ( B , D ) visualization of α2 (red) in crypts and villus; ( E , F ) control sections labeled with secondary antibody [Alexa Fluor 546 donkey anti-(rabbit IgG)] in the absence of primary antibody; ( G , I ) visualization of β4 (red) in crypts and villus; ( J , K ) control sections labeled with secondary antibody [Alexa Fluor 568 rabbit anti-(goat IgG)] in the absence of primary antibody; ( L – O ) co-localization of α2 and β4 in crypts; ( M ) visualization of α2 (green) in crypts; ( N ) visualization of β4 (red) in crypts; ( O ) merged visualization of ( L ), ( M ), and ( N ). ( C , H , P ) Enlargement of ( B ), ( G ), and ( O ). White dotted lines indicate the crypt region. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( B – P ) except for ( C ), ( H ), and ( P ) represent 20 μm.
    Figure Legend Snippet: Localization of nAChR subunits in the mouse small intestine and organoids. ( A ) RT-PCR analysis of the expression of nAChR subunits in intestine and cultured organoids (passage 5); ( B , D ) visualization of α2 (red) in crypts and villus; ( E , F ) control sections labeled with secondary antibody [Alexa Fluor 546 donkey anti-(rabbit IgG)] in the absence of primary antibody; ( G , I ) visualization of β4 (red) in crypts and villus; ( J , K ) control sections labeled with secondary antibody [Alexa Fluor 568 rabbit anti-(goat IgG)] in the absence of primary antibody; ( L – O ) co-localization of α2 and β4 in crypts; ( M ) visualization of α2 (green) in crypts; ( N ) visualization of β4 (red) in crypts; ( O ) merged visualization of ( L ), ( M ), and ( N ). ( C , H , P ) Enlargement of ( B ), ( G ), and ( O ). White dotted lines indicate the crypt region. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( B – P ) except for ( C ), ( H ), and ( P ) represent 20 μm.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing, Cell Culture, Labeling, Staining

    Model depicting the proposed role of nAChR signaling in intestinal stem cell function. Non-neuronal ACh activates the nicotinic receptor α2/β4 localized in the Paneth cells to modulate the expression levels of Wnt5a and/or Wnt9b . The Wnts then mediate Wnt pathway activity through various frizzled receptors. Eventually, proliferation and differentiation of stem cells are enhanced.
    Figure Legend Snippet: Model depicting the proposed role of nAChR signaling in intestinal stem cell function. Non-neuronal ACh activates the nicotinic receptor α2/β4 localized in the Paneth cells to modulate the expression levels of Wnt5a and/or Wnt9b . The Wnts then mediate Wnt pathway activity through various frizzled receptors. Eventually, proliferation and differentiation of stem cells are enhanced.

    Techniques Used: Cell Function Assay, Expressing, Activity Assay

    Co-localization and gene expression of α2 and β4 in Paneth cells. ( A ) Visualization of lysozyme (green) in crypts; ( B ) visualization of α2 (red) in crypts; ( C ) merged visualization of ( A ) and ( B ); ( D ) visualization of lysozyme (red) in crypts; ( E ) visualization of β4 (green) in crypts; ( F ) merged visualization of ( D ) and ( E ). White dotted lines indicate the crypt region. Orange dotted lines indicate the typical cell. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( A – F ) represent 20 μm. ( G ) FACS plot of dissociated single cells from organoid tissues. Two CD24 bright populations differ by side-scatter (SSC) pattern. Sorted CD24 high /SSC low (P3) and CD24 high /SSC high (P2) cells are subsequently stained. CD24 high /SSC low cells are positive for the enteroendocrine marker chromogranine A (top right), whereas CD24 high /SSC high cells are positive for the Paneth marker lysozyme (bottom right). ( H ) CD24 high /SSC high (P2) and CD24 high /SSC low (P3) cells were examined for transcripts of α2 ( Alpha2 ) and β4 ( Beta4 ) with quantitative RT-PCR. The results are based on three independent experiments as mean values ± SD. The statistical significance was calculated with Student’s t -test (** p
    Figure Legend Snippet: Co-localization and gene expression of α2 and β4 in Paneth cells. ( A ) Visualization of lysozyme (green) in crypts; ( B ) visualization of α2 (red) in crypts; ( C ) merged visualization of ( A ) and ( B ); ( D ) visualization of lysozyme (red) in crypts; ( E ) visualization of β4 (green) in crypts; ( F ) merged visualization of ( D ) and ( E ). White dotted lines indicate the crypt region. Orange dotted lines indicate the typical cell. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( A – F ) represent 20 μm. ( G ) FACS plot of dissociated single cells from organoid tissues. Two CD24 bright populations differ by side-scatter (SSC) pattern. Sorted CD24 high /SSC low (P3) and CD24 high /SSC high (P2) cells are subsequently stained. CD24 high /SSC low cells are positive for the enteroendocrine marker chromogranine A (top right), whereas CD24 high /SSC high cells are positive for the Paneth marker lysozyme (bottom right). ( H ) CD24 high /SSC high (P2) and CD24 high /SSC low (P3) cells were examined for transcripts of α2 ( Alpha2 ) and β4 ( Beta4 ) with quantitative RT-PCR. The results are based on three independent experiments as mean values ± SD. The statistical significance was calculated with Student’s t -test (** p

    Techniques Used: Expressing, Staining, FACS, Marker, Quantitative RT-PCR

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    Alomone Labs anti nicotinic ach receptor α2
    Localization of nAChR subunits in the mouse small intestine and organoids. ( A ) RT-PCR analysis of the expression of nAChR subunits in intestine and cultured organoids (passage 5); ( B , D ) visualization of <t>α2</t> (red) in crypts and villus; ( E , F ) control sections labeled with secondary antibody [Alexa Fluor 546 donkey anti-(rabbit IgG)] in the absence of primary antibody; ( G , I ) visualization of β4 (red) in crypts and villus; ( J , K ) control sections labeled with secondary antibody [Alexa Fluor 568 rabbit anti-(goat IgG)] in the absence of primary antibody; ( L – O ) co-localization of α2 and β4 in crypts; ( M ) visualization of α2 (green) in crypts; ( N ) visualization of β4 (red) in crypts; ( O ) merged visualization of ( L ), ( M ), and ( N ). ( C , H , P ) Enlargement of ( B ), ( G ), and ( O ). White dotted lines indicate the crypt region. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( B – P ) except for ( C ), ( H ), and ( P ) represent 20 μm.
    Anti Nicotinic Ach Receptor α2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nicotinic ach receptor α2/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti nicotinic ach receptor α2 - by Bioz Stars, 2022-01
    91/100 stars
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    Localization of nAChR subunits in the mouse small intestine and organoids. ( A ) RT-PCR analysis of the expression of nAChR subunits in intestine and cultured organoids (passage 5); ( B , D ) visualization of α2 (red) in crypts and villus; ( E , F ) control sections labeled with secondary antibody [Alexa Fluor 546 donkey anti-(rabbit IgG)] in the absence of primary antibody; ( G , I ) visualization of β4 (red) in crypts and villus; ( J , K ) control sections labeled with secondary antibody [Alexa Fluor 568 rabbit anti-(goat IgG)] in the absence of primary antibody; ( L – O ) co-localization of α2 and β4 in crypts; ( M ) visualization of α2 (green) in crypts; ( N ) visualization of β4 (red) in crypts; ( O ) merged visualization of ( L ), ( M ), and ( N ). ( C , H , P ) Enlargement of ( B ), ( G ), and ( O ). White dotted lines indicate the crypt region. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( B – P ) except for ( C ), ( H ), and ( P ) represent 20 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice

    doi: 10.3390/ijms19030738

    Figure Lengend Snippet: Localization of nAChR subunits in the mouse small intestine and organoids. ( A ) RT-PCR analysis of the expression of nAChR subunits in intestine and cultured organoids (passage 5); ( B , D ) visualization of α2 (red) in crypts and villus; ( E , F ) control sections labeled with secondary antibody [Alexa Fluor 546 donkey anti-(rabbit IgG)] in the absence of primary antibody; ( G , I ) visualization of β4 (red) in crypts and villus; ( J , K ) control sections labeled with secondary antibody [Alexa Fluor 568 rabbit anti-(goat IgG)] in the absence of primary antibody; ( L – O ) co-localization of α2 and β4 in crypts; ( M ) visualization of α2 (green) in crypts; ( N ) visualization of β4 (red) in crypts; ( O ) merged visualization of ( L ), ( M ), and ( N ). ( C , H , P ) Enlargement of ( B ), ( G ), and ( O ). White dotted lines indicate the crypt region. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( B – P ) except for ( C ), ( H ), and ( P ) represent 20 μm.

    Article Snippet: The primary antibodies used for immunohistochemistry were as follows: anti-nicotinic ACh receptor α2 (1:1000; Alomone Labs, Jerusalem, Israel), anti-nicotinic ACh receptor β4 (1:1000; Abcam, Tokyo, Japan), two types of anti-lysozyme antibody (1:1000; Abcam, Cat No. ab36362 and ab108508), and anti-chromogranin A antibody (1:1000; Abcam).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Cell Culture, Labeling, Staining

    Model depicting the proposed role of nAChR signaling in intestinal stem cell function. Non-neuronal ACh activates the nicotinic receptor α2/β4 localized in the Paneth cells to modulate the expression levels of Wnt5a and/or Wnt9b . The Wnts then mediate Wnt pathway activity through various frizzled receptors. Eventually, proliferation and differentiation of stem cells are enhanced.

    Journal: International Journal of Molecular Sciences

    Article Title: The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice

    doi: 10.3390/ijms19030738

    Figure Lengend Snippet: Model depicting the proposed role of nAChR signaling in intestinal stem cell function. Non-neuronal ACh activates the nicotinic receptor α2/β4 localized in the Paneth cells to modulate the expression levels of Wnt5a and/or Wnt9b . The Wnts then mediate Wnt pathway activity through various frizzled receptors. Eventually, proliferation and differentiation of stem cells are enhanced.

    Article Snippet: The primary antibodies used for immunohistochemistry were as follows: anti-nicotinic ACh receptor α2 (1:1000; Alomone Labs, Jerusalem, Israel), anti-nicotinic ACh receptor β4 (1:1000; Abcam, Tokyo, Japan), two types of anti-lysozyme antibody (1:1000; Abcam, Cat No. ab36362 and ab108508), and anti-chromogranin A antibody (1:1000; Abcam).

    Techniques: Cell Function Assay, Expressing, Activity Assay

    Co-localization and gene expression of α2 and β4 in Paneth cells. ( A ) Visualization of lysozyme (green) in crypts; ( B ) visualization of α2 (red) in crypts; ( C ) merged visualization of ( A ) and ( B ); ( D ) visualization of lysozyme (red) in crypts; ( E ) visualization of β4 (green) in crypts; ( F ) merged visualization of ( D ) and ( E ). White dotted lines indicate the crypt region. Orange dotted lines indicate the typical cell. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( A – F ) represent 20 μm. ( G ) FACS plot of dissociated single cells from organoid tissues. Two CD24 bright populations differ by side-scatter (SSC) pattern. Sorted CD24 high /SSC low (P3) and CD24 high /SSC high (P2) cells are subsequently stained. CD24 high /SSC low cells are positive for the enteroendocrine marker chromogranine A (top right), whereas CD24 high /SSC high cells are positive for the Paneth marker lysozyme (bottom right). ( H ) CD24 high /SSC high (P2) and CD24 high /SSC low (P3) cells were examined for transcripts of α2 ( Alpha2 ) and β4 ( Beta4 ) with quantitative RT-PCR. The results are based on three independent experiments as mean values ± SD. The statistical significance was calculated with Student’s t -test (** p

    Journal: International Journal of Molecular Sciences

    Article Title: The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice

    doi: 10.3390/ijms19030738

    Figure Lengend Snippet: Co-localization and gene expression of α2 and β4 in Paneth cells. ( A ) Visualization of lysozyme (green) in crypts; ( B ) visualization of α2 (red) in crypts; ( C ) merged visualization of ( A ) and ( B ); ( D ) visualization of lysozyme (red) in crypts; ( E ) visualization of β4 (green) in crypts; ( F ) merged visualization of ( D ) and ( E ). White dotted lines indicate the crypt region. Orange dotted lines indicate the typical cell. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( A – F ) represent 20 μm. ( G ) FACS plot of dissociated single cells from organoid tissues. Two CD24 bright populations differ by side-scatter (SSC) pattern. Sorted CD24 high /SSC low (P3) and CD24 high /SSC high (P2) cells are subsequently stained. CD24 high /SSC low cells are positive for the enteroendocrine marker chromogranine A (top right), whereas CD24 high /SSC high cells are positive for the Paneth marker lysozyme (bottom right). ( H ) CD24 high /SSC high (P2) and CD24 high /SSC low (P3) cells were examined for transcripts of α2 ( Alpha2 ) and β4 ( Beta4 ) with quantitative RT-PCR. The results are based on three independent experiments as mean values ± SD. The statistical significance was calculated with Student’s t -test (** p

    Article Snippet: The primary antibodies used for immunohistochemistry were as follows: anti-nicotinic ACh receptor α2 (1:1000; Alomone Labs, Jerusalem, Israel), anti-nicotinic ACh receptor β4 (1:1000; Abcam, Tokyo, Japan), two types of anti-lysozyme antibody (1:1000; Abcam, Cat No. ab36362 and ab108508), and anti-chromogranin A antibody (1:1000; Abcam).

    Techniques: Expressing, Staining, FACS, Marker, Quantitative RT-PCR