anti nicotinic ach receptor α2  (Alomone Labs)


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    Name:
    Anti Nicotinic Acetylcholine Receptor alpha2 CHRNA2 extracellular Antibody
    Description:
    Anti Nicotinic Acetylcholine Receptor alpha2 CHRNA2 extracellular Antibody is directed against an extracellular epitope of rat nAChRα2 Anti Nicotinic Acetylcholine Receptor α2 extracellular CHRNA2 Antibody ANC 002 can be used in western blot immunohistochemistry and immunocytochemistry applications The antibody is specially suited to recognize nAChRα2 in live cells It has been designed to recognize nAChRα2 from rat mouse and human samples
    Catalog Number:
    ANC-002
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs anti nicotinic ach receptor α2
    Anti Nicotinic Acetylcholine Receptor alpha2 CHRNA2 extracellular Antibody
    Anti Nicotinic Acetylcholine Receptor alpha2 CHRNA2 extracellular Antibody is directed against an extracellular epitope of rat nAChRα2 Anti Nicotinic Acetylcholine Receptor α2 extracellular CHRNA2 Antibody ANC 002 can be used in western blot immunohistochemistry and immunocytochemistry applications The antibody is specially suited to recognize nAChRα2 in live cells It has been designed to recognize nAChRα2 from rat mouse and human samples
    https://www.bioz.com/result/anti nicotinic ach receptor α2/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti nicotinic ach receptor α2 - by Bioz Stars, 2021-09
    91/100 stars

    Images

    1) Product Images from "The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice"

    Article Title: The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19030738

    Localization of nAChR subunits in the mouse small intestine and organoids. ( A ) RT-PCR analysis of the expression of nAChR subunits in intestine and cultured organoids (passage 5); ( B , D ) visualization of α2 (red) in crypts and villus; ( E , F ) control sections labeled with secondary antibody [Alexa Fluor 546 donkey anti-(rabbit IgG)] in the absence of primary antibody; ( G , I ) visualization of β4 (red) in crypts and villus; ( J , K ) control sections labeled with secondary antibody [Alexa Fluor 568 rabbit anti-(goat IgG)] in the absence of primary antibody; ( L – O ) co-localization of α2 and β4 in crypts; ( M ) visualization of α2 (green) in crypts; ( N ) visualization of β4 (red) in crypts; ( O ) merged visualization of ( L ), ( M ), and ( N ). ( C , H , P ) Enlargement of ( B ), ( G ), and ( O ). White dotted lines indicate the crypt region. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( B – P ) except for ( C ), ( H ), and ( P ) represent 20 μm.
    Figure Legend Snippet: Localization of nAChR subunits in the mouse small intestine and organoids. ( A ) RT-PCR analysis of the expression of nAChR subunits in intestine and cultured organoids (passage 5); ( B , D ) visualization of α2 (red) in crypts and villus; ( E , F ) control sections labeled with secondary antibody [Alexa Fluor 546 donkey anti-(rabbit IgG)] in the absence of primary antibody; ( G , I ) visualization of β4 (red) in crypts and villus; ( J , K ) control sections labeled with secondary antibody [Alexa Fluor 568 rabbit anti-(goat IgG)] in the absence of primary antibody; ( L – O ) co-localization of α2 and β4 in crypts; ( M ) visualization of α2 (green) in crypts; ( N ) visualization of β4 (red) in crypts; ( O ) merged visualization of ( L ), ( M ), and ( N ). ( C , H , P ) Enlargement of ( B ), ( G ), and ( O ). White dotted lines indicate the crypt region. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( B – P ) except for ( C ), ( H ), and ( P ) represent 20 μm.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing, Cell Culture, Labeling, Staining

    Model depicting the proposed role of nAChR signaling in intestinal stem cell function. Non-neuronal ACh activates the nicotinic receptor α2/β4 localized in the Paneth cells to modulate the expression levels of Wnt5a and/or Wnt9b . The Wnts then mediate Wnt pathway activity through various frizzled receptors. Eventually, proliferation and differentiation of stem cells are enhanced.
    Figure Legend Snippet: Model depicting the proposed role of nAChR signaling in intestinal stem cell function. Non-neuronal ACh activates the nicotinic receptor α2/β4 localized in the Paneth cells to modulate the expression levels of Wnt5a and/or Wnt9b . The Wnts then mediate Wnt pathway activity through various frizzled receptors. Eventually, proliferation and differentiation of stem cells are enhanced.

    Techniques Used: Cell Function Assay, Expressing, Activity Assay

    Co-localization and gene expression of α2 and β4 in Paneth cells. ( A ) Visualization of lysozyme (green) in crypts; ( B ) visualization of α2 (red) in crypts; ( C ) merged visualization of ( A ) and ( B ); ( D ) visualization of lysozyme (red) in crypts; ( E ) visualization of β4 (green) in crypts; ( F ) merged visualization of ( D ) and ( E ). White dotted lines indicate the crypt region. Orange dotted lines indicate the typical cell. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( A – F ) represent 20 μm. ( G ) FACS plot of dissociated single cells from organoid tissues. Two CD24 bright populations differ by side-scatter (SSC) pattern. Sorted CD24 high /SSC low (P3) and CD24 high /SSC high (P2) cells are subsequently stained. CD24 high /SSC low cells are positive for the enteroendocrine marker chromogranine A (top right), whereas CD24 high /SSC high cells are positive for the Paneth marker lysozyme (bottom right). ( H ) CD24 high /SSC high (P2) and CD24 high /SSC low (P3) cells were examined for transcripts of α2 ( Alpha2 ) and β4 ( Beta4 ) with quantitative RT-PCR. The results are based on three independent experiments as mean values ± SD. The statistical significance was calculated with Student’s t -test (** p
    Figure Legend Snippet: Co-localization and gene expression of α2 and β4 in Paneth cells. ( A ) Visualization of lysozyme (green) in crypts; ( B ) visualization of α2 (red) in crypts; ( C ) merged visualization of ( A ) and ( B ); ( D ) visualization of lysozyme (red) in crypts; ( E ) visualization of β4 (green) in crypts; ( F ) merged visualization of ( D ) and ( E ). White dotted lines indicate the crypt region. Orange dotted lines indicate the typical cell. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( A – F ) represent 20 μm. ( G ) FACS plot of dissociated single cells from organoid tissues. Two CD24 bright populations differ by side-scatter (SSC) pattern. Sorted CD24 high /SSC low (P3) and CD24 high /SSC high (P2) cells are subsequently stained. CD24 high /SSC low cells are positive for the enteroendocrine marker chromogranine A (top right), whereas CD24 high /SSC high cells are positive for the Paneth marker lysozyme (bottom right). ( H ) CD24 high /SSC high (P2) and CD24 high /SSC low (P3) cells were examined for transcripts of α2 ( Alpha2 ) and β4 ( Beta4 ) with quantitative RT-PCR. The results are based on three independent experiments as mean values ± SD. The statistical significance was calculated with Student’s t -test (** p

    Techniques Used: Expressing, Staining, FACS, Marker, Quantitative RT-PCR

    Related Articles

    Immunohistochemistry:

    Article Title: The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice
    Article Snippet: .. The primary antibodies used for immunohistochemistry were as follows: anti-nicotinic ACh receptor α2 (1:1000; Alomone Labs, Jerusalem, Israel), anti-nicotinic ACh receptor β4 (1:1000; Abcam, Tokyo, Japan), two types of anti-lysozyme antibody (1:1000; Abcam, Cat No. ab36362 and ab108508), and anti-chromogranin A antibody (1:1000; Abcam). ..

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  • 91
    Alomone Labs anti nicotinic ach receptor α2
    Localization of nAChR subunits in the mouse small intestine and organoids. ( A ) RT-PCR analysis of the expression of nAChR subunits in intestine and cultured organoids (passage 5); ( B , D ) visualization of <t>α2</t> (red) in crypts and villus; ( E , F ) control sections labeled with secondary antibody [Alexa Fluor 546 donkey anti-(rabbit IgG)] in the absence of primary antibody; ( G , I ) visualization of β4 (red) in crypts and villus; ( J , K ) control sections labeled with secondary antibody [Alexa Fluor 568 rabbit anti-(goat IgG)] in the absence of primary antibody; ( L – O ) co-localization of α2 and β4 in crypts; ( M ) visualization of α2 (green) in crypts; ( N ) visualization of β4 (red) in crypts; ( O ) merged visualization of ( L ), ( M ), and ( N ). ( C , H , P ) Enlargement of ( B ), ( G ), and ( O ). White dotted lines indicate the crypt region. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( B – P ) except for ( C ), ( H ), and ( P ) represent 20 μm.
    Anti Nicotinic Ach Receptor α2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nicotinic ach receptor α2/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti nicotinic ach receptor α2 - by Bioz Stars, 2021-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    Localization of nAChR subunits in the mouse small intestine and organoids. ( A ) RT-PCR analysis of the expression of nAChR subunits in intestine and cultured organoids (passage 5); ( B , D ) visualization of α2 (red) in crypts and villus; ( E , F ) control sections labeled with secondary antibody [Alexa Fluor 546 donkey anti-(rabbit IgG)] in the absence of primary antibody; ( G , I ) visualization of β4 (red) in crypts and villus; ( J , K ) control sections labeled with secondary antibody [Alexa Fluor 568 rabbit anti-(goat IgG)] in the absence of primary antibody; ( L – O ) co-localization of α2 and β4 in crypts; ( M ) visualization of α2 (green) in crypts; ( N ) visualization of β4 (red) in crypts; ( O ) merged visualization of ( L ), ( M ), and ( N ). ( C , H , P ) Enlargement of ( B ), ( G ), and ( O ). White dotted lines indicate the crypt region. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( B – P ) except for ( C ), ( H ), and ( P ) represent 20 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice

    doi: 10.3390/ijms19030738

    Figure Lengend Snippet: Localization of nAChR subunits in the mouse small intestine and organoids. ( A ) RT-PCR analysis of the expression of nAChR subunits in intestine and cultured organoids (passage 5); ( B , D ) visualization of α2 (red) in crypts and villus; ( E , F ) control sections labeled with secondary antibody [Alexa Fluor 546 donkey anti-(rabbit IgG)] in the absence of primary antibody; ( G , I ) visualization of β4 (red) in crypts and villus; ( J , K ) control sections labeled with secondary antibody [Alexa Fluor 568 rabbit anti-(goat IgG)] in the absence of primary antibody; ( L – O ) co-localization of α2 and β4 in crypts; ( M ) visualization of α2 (green) in crypts; ( N ) visualization of β4 (red) in crypts; ( O ) merged visualization of ( L ), ( M ), and ( N ). ( C , H , P ) Enlargement of ( B ), ( G ), and ( O ). White dotted lines indicate the crypt region. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( B – P ) except for ( C ), ( H ), and ( P ) represent 20 μm.

    Article Snippet: The primary antibodies used for immunohistochemistry were as follows: anti-nicotinic ACh receptor α2 (1:1000; Alomone Labs, Jerusalem, Israel), anti-nicotinic ACh receptor β4 (1:1000; Abcam, Tokyo, Japan), two types of anti-lysozyme antibody (1:1000; Abcam, Cat No. ab36362 and ab108508), and anti-chromogranin A antibody (1:1000; Abcam).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Cell Culture, Labeling, Staining

    Model depicting the proposed role of nAChR signaling in intestinal stem cell function. Non-neuronal ACh activates the nicotinic receptor α2/β4 localized in the Paneth cells to modulate the expression levels of Wnt5a and/or Wnt9b . The Wnts then mediate Wnt pathway activity through various frizzled receptors. Eventually, proliferation and differentiation of stem cells are enhanced.

    Journal: International Journal of Molecular Sciences

    Article Title: The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice

    doi: 10.3390/ijms19030738

    Figure Lengend Snippet: Model depicting the proposed role of nAChR signaling in intestinal stem cell function. Non-neuronal ACh activates the nicotinic receptor α2/β4 localized in the Paneth cells to modulate the expression levels of Wnt5a and/or Wnt9b . The Wnts then mediate Wnt pathway activity through various frizzled receptors. Eventually, proliferation and differentiation of stem cells are enhanced.

    Article Snippet: The primary antibodies used for immunohistochemistry were as follows: anti-nicotinic ACh receptor α2 (1:1000; Alomone Labs, Jerusalem, Israel), anti-nicotinic ACh receptor β4 (1:1000; Abcam, Tokyo, Japan), two types of anti-lysozyme antibody (1:1000; Abcam, Cat No. ab36362 and ab108508), and anti-chromogranin A antibody (1:1000; Abcam).

    Techniques: Cell Function Assay, Expressing, Activity Assay

    Co-localization and gene expression of α2 and β4 in Paneth cells. ( A ) Visualization of lysozyme (green) in crypts; ( B ) visualization of α2 (red) in crypts; ( C ) merged visualization of ( A ) and ( B ); ( D ) visualization of lysozyme (red) in crypts; ( E ) visualization of β4 (green) in crypts; ( F ) merged visualization of ( D ) and ( E ). White dotted lines indicate the crypt region. Orange dotted lines indicate the typical cell. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( A – F ) represent 20 μm. ( G ) FACS plot of dissociated single cells from organoid tissues. Two CD24 bright populations differ by side-scatter (SSC) pattern. Sorted CD24 high /SSC low (P3) and CD24 high /SSC high (P2) cells are subsequently stained. CD24 high /SSC low cells are positive for the enteroendocrine marker chromogranine A (top right), whereas CD24 high /SSC high cells are positive for the Paneth marker lysozyme (bottom right). ( H ) CD24 high /SSC high (P2) and CD24 high /SSC low (P3) cells were examined for transcripts of α2 ( Alpha2 ) and β4 ( Beta4 ) with quantitative RT-PCR. The results are based on three independent experiments as mean values ± SD. The statistical significance was calculated with Student’s t -test (** p

    Journal: International Journal of Molecular Sciences

    Article Title: The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice

    doi: 10.3390/ijms19030738

    Figure Lengend Snippet: Co-localization and gene expression of α2 and β4 in Paneth cells. ( A ) Visualization of lysozyme (green) in crypts; ( B ) visualization of α2 (red) in crypts; ( C ) merged visualization of ( A ) and ( B ); ( D ) visualization of lysozyme (red) in crypts; ( E ) visualization of β4 (green) in crypts; ( F ) merged visualization of ( D ) and ( E ). White dotted lines indicate the crypt region. Orange dotted lines indicate the typical cell. In all panels, nuclei were stained with Hoechst 33342 (blue). Bars in ( A – F ) represent 20 μm. ( G ) FACS plot of dissociated single cells from organoid tissues. Two CD24 bright populations differ by side-scatter (SSC) pattern. Sorted CD24 high /SSC low (P3) and CD24 high /SSC high (P2) cells are subsequently stained. CD24 high /SSC low cells are positive for the enteroendocrine marker chromogranine A (top right), whereas CD24 high /SSC high cells are positive for the Paneth marker lysozyme (bottom right). ( H ) CD24 high /SSC high (P2) and CD24 high /SSC low (P3) cells were examined for transcripts of α2 ( Alpha2 ) and β4 ( Beta4 ) with quantitative RT-PCR. The results are based on three independent experiments as mean values ± SD. The statistical significance was calculated with Student’s t -test (** p

    Article Snippet: The primary antibodies used for immunohistochemistry were as follows: anti-nicotinic ACh receptor α2 (1:1000; Alomone Labs, Jerusalem, Israel), anti-nicotinic ACh receptor β4 (1:1000; Abcam, Tokyo, Japan), two types of anti-lysozyme antibody (1:1000; Abcam, Cat No. ab36362 and ab108508), and anti-chromogranin A antibody (1:1000; Abcam).

    Techniques: Expressing, Staining, FACS, Marker, Quantitative RT-PCR