anti kv3 2  (Alomone Labs)

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

Article Title: Fast delayed rectifier potassium current: critical for input and output of the circadian system

doi: 10.1523/JNEUROSCI.5792-10.2011

Figure Lengend Snippet: Kv3.1 and Kv3.2 channel proteins are not expressed in the SCN of mice deficient in both Kcnc1 and Kcnc2 . IHC was used to determine expression of Kv3.1 and Kv3.2 channel proteins in the mouse SCN. Panels show photomicrograms (400×) of immuno-reactivity seen with each of the three genotypes: C57BL/6 (WT, top panels), Kcnc2 -null (sKO, middle panels), Kcnc1 - and Kcnc2 -null (dKO, bottom panels). The sKO and dKO mice shown in this and subsequent figures are littermates on an ICR background. Tissue was collected at ZT 6. The scale bar represents 100 µm.

Article Snippet: Sections were then washed again in PBS (three times), dipped in 3 % normal goat serum in PBS with 0.1 % Triton X-100 for 1 hr, and then incubated with a rabbit anti-Kv3.1 (1:200), anti-Kv3.2 (1:75; Alomone Labs, Jerusalem, Israel), anti-cFOS (1:30,000; Merck, Darmstadt, Germany) or a rabbit anti-PER2 antiserum (1:1000; Alpha Diagnostics, San Antonio, TX) in PBS (3% normal goat serum, 0.1 % Triton X-100) at 4 °C overnight.

Techniques: Mouse Assay, Immunohistochemistry, Expressing

Journal: The Journal of Neuroscience

Article Title: K+ Channel Kv3.4 Is Essential for Axon Growth by Limiting the Influx of Ca2+ into Growth Cones

doi: 10.1523/JNEUROSCI.1076-16.2017

Figure Lengend Snippet: Kv3.4 in the axonal growth cones of dorsal spinal commissural neurons. A–F , Transverse sections of the spinal cord of chick embryos were immunostained for Kv3.4. A , Absence of Kv3.4-IR in the dorsal spinal cord at HH17. Kv3.4-IR in precrossing commissural axons ( B–F , arrowheads) is evident during HH19-HH25 but disappears at HH27. D , Arrows indicate postcrossing commissural axons projecting from the other side of spinal cord. FP, Floor plate. G–L , Transverse sections of the spinal cord at HH23 were immunostained as indicated. G , Absence of Kv1.5-IR. H , Kv4.2-IR in the somata and dendrites of motoneurons (MN). I , Kv4.3-IR in the bifurcation zone (BZ). In addition to the BZ, Kv3.1b-IR is strong in postcrossing commissural axons ( J , arrow) but weak in precrossing commissural axons ( J , arrowhead). K , Absence of Kv3.2-IR. L , Kv3.3 in motoneurons. M–M″ , Double staining in transverse sections of the spinal cord at HH21 shows colocalization of Kv3.4 and axonin-1 in the growth cones (arrowheads) of commissural axons. N–N″ , Colocalization of Kv3.4 and axonin-1 in cultured dorsal spinal neurons isolated from HH21-HH23 chick embryos. O–P″ , Red fluorescence-tagged phalloidin colabeling reveals enrichment of Kv3.4 in the growth cone ( O–O″ ) and Kv3.1b in the soma/axon shaft ( P–P″ ) of cultured dorsal spinal neurons. Q–Q″ , Kv3.4 and DiI colabeling. White represents Kv3.4-abundant regions. Blue represents Kv3.4-sparse regions ( Q″ ). R , Ratio of Kv3.4/DiI in the soma, axon shaft, or growth cone of each neuron was obtained by dividing the fluorescence intensity of Kv3.4 by that of DiI. Data are mean ± SEM ( n = 8 neurons, pooled from three independent experiments done on different days). *** p

Article Snippet: The specificity of anti-Kv3.2 (Alomone Labs catalog #APC-011, RRID:AB_2040168), anti-Kv3.3 (Alomone Labs catalog #APC-102, RRID:AB_2040170), and anti-Kv3.4 (Alomone Labs catalog #APC-019, RRID:AB_ 2040172) has been described previously ( ).

Techniques: Double Staining, Cell Culture, Isolation, Fluorescence