anti vmat2 antibody  (Alomone Labs)


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    Alomone Labs anti vmat2 antibody
    DAT expression and function in DAT-P2A-Flpo mice (A) Representative western blot images of DAT, TH, vesicular monoamine transporter 2 <t>(VMAT2),</t> and histone H3 (H3) loading control from striatal lysates from DAT-P2A-Flpo WT and heterozygous (Het) mice. Two independent samples per genotype are shown. Blots were cropped to show the relevant bands. Molecular weight (MW) in kDa is indicated on the right. Representative of 4 mice.(B) Quantification of protein levels relative to H3, normalized to WT. Bars represent mean ± SEM. Each dot represents the average of two striatal samples from one mouse (n = 4 mice per genotype, 2 males and 2 females, P100–120). DAT, *p = 0.0259; TH, p = 0.9639; VMAT2, p = 0.0930; unpaired t tests. (C) Extracellular DA ([DA] o ) transients evoked by single electrical pulses and recorded with fast-scan cyclic voltammetry (FCV) in different striatal sub-regions. Traces are mean ± SEM [DA] o versus time (average of 32 transients per recording site from 4 pairs of DAT-P2A-Flpo WT and Het mice, 2 male pairs and 2 female pairs, P100–120). 1, ventromedial striatum; 2, dorsomedial striatum; 3, dorsolateral striatum; 4, central striatum; 5, ventrolateral striatum; 6+7, nucleus accumbens (NAc). (D) Mean ± SEM [DA] o versus time (average of 80 transients per genotype) from the dorsal striatum (dStr) sites 1–5. (E) Mean ± SEM [DA] o versus time (average of 32 transients per genotype) from the NAc sites 6+7. (F) Mean ± SEM [DA] o of selected region- and peak-matched transients from the dStr of DAT-P2A-Flpo WT and Het mice (average of 22 transients per genotype). (G) Single exponential curve fit of the decay phase of the transients in (F). DAT-P2A-Flpo Het mice have significantly slower [DA] o re-uptake than WT mice (***p
    Anti Vmat2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti vmat2 antibody/product/Alomone Labs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti vmat2 antibody - by Bioz Stars, 2022-11
    94/100 stars

    Images

    1) Product Images from "Generation of a DAT-P2A-Flpo mouse line for intersectional genetic targeting of dopamine neuron subpopulations"

    Article Title: Generation of a DAT-P2A-Flpo mouse line for intersectional genetic targeting of dopamine neuron subpopulations

    Journal: Cell reports

    doi: 10.1016/j.celrep.2021.109123

    DAT expression and function in DAT-P2A-Flpo mice (A) Representative western blot images of DAT, TH, vesicular monoamine transporter 2 (VMAT2), and histone H3 (H3) loading control from striatal lysates from DAT-P2A-Flpo WT and heterozygous (Het) mice. Two independent samples per genotype are shown. Blots were cropped to show the relevant bands. Molecular weight (MW) in kDa is indicated on the right. Representative of 4 mice.(B) Quantification of protein levels relative to H3, normalized to WT. Bars represent mean ± SEM. Each dot represents the average of two striatal samples from one mouse (n = 4 mice per genotype, 2 males and 2 females, P100–120). DAT, *p = 0.0259; TH, p = 0.9639; VMAT2, p = 0.0930; unpaired t tests. (C) Extracellular DA ([DA] o ) transients evoked by single electrical pulses and recorded with fast-scan cyclic voltammetry (FCV) in different striatal sub-regions. Traces are mean ± SEM [DA] o versus time (average of 32 transients per recording site from 4 pairs of DAT-P2A-Flpo WT and Het mice, 2 male pairs and 2 female pairs, P100–120). 1, ventromedial striatum; 2, dorsomedial striatum; 3, dorsolateral striatum; 4, central striatum; 5, ventrolateral striatum; 6+7, nucleus accumbens (NAc). (D) Mean ± SEM [DA] o versus time (average of 80 transients per genotype) from the dorsal striatum (dStr) sites 1–5. (E) Mean ± SEM [DA] o versus time (average of 32 transients per genotype) from the NAc sites 6+7. (F) Mean ± SEM [DA] o of selected region- and peak-matched transients from the dStr of DAT-P2A-Flpo WT and Het mice (average of 22 transients per genotype). (G) Single exponential curve fit of the decay phase of the transients in (F). DAT-P2A-Flpo Het mice have significantly slower [DA] o re-uptake than WT mice (***p
    Figure Legend Snippet: DAT expression and function in DAT-P2A-Flpo mice (A) Representative western blot images of DAT, TH, vesicular monoamine transporter 2 (VMAT2), and histone H3 (H3) loading control from striatal lysates from DAT-P2A-Flpo WT and heterozygous (Het) mice. Two independent samples per genotype are shown. Blots were cropped to show the relevant bands. Molecular weight (MW) in kDa is indicated on the right. Representative of 4 mice.(B) Quantification of protein levels relative to H3, normalized to WT. Bars represent mean ± SEM. Each dot represents the average of two striatal samples from one mouse (n = 4 mice per genotype, 2 males and 2 females, P100–120). DAT, *p = 0.0259; TH, p = 0.9639; VMAT2, p = 0.0930; unpaired t tests. (C) Extracellular DA ([DA] o ) transients evoked by single electrical pulses and recorded with fast-scan cyclic voltammetry (FCV) in different striatal sub-regions. Traces are mean ± SEM [DA] o versus time (average of 32 transients per recording site from 4 pairs of DAT-P2A-Flpo WT and Het mice, 2 male pairs and 2 female pairs, P100–120). 1, ventromedial striatum; 2, dorsomedial striatum; 3, dorsolateral striatum; 4, central striatum; 5, ventrolateral striatum; 6+7, nucleus accumbens (NAc). (D) Mean ± SEM [DA] o versus time (average of 80 transients per genotype) from the dorsal striatum (dStr) sites 1–5. (E) Mean ± SEM [DA] o versus time (average of 32 transients per genotype) from the NAc sites 6+7. (F) Mean ± SEM [DA] o of selected region- and peak-matched transients from the dStr of DAT-P2A-Flpo WT and Het mice (average of 22 transients per genotype). (G) Single exponential curve fit of the decay phase of the transients in (F). DAT-P2A-Flpo Het mice have significantly slower [DA] o re-uptake than WT mice (***p

    Techniques Used: Expressing, Mouse Assay, Western Blot, Molecular Weight

    2) Product Images from "Generation of a DAT-P2A-Flpo mouse line for intersectional genetic targeting of dopamine neuron subpopulations"

    Article Title: Generation of a DAT-P2A-Flpo mouse line for intersectional genetic targeting of dopamine neuron subpopulations

    Journal: Cell reports

    doi: 10.1016/j.celrep.2021.109123

    DAT expression and function in DAT-P2A-Flpo mice (A) Representative western blot images of DAT, TH, vesicular monoamine transporter 2 (VMAT2), and histone H3 (H3) loading control from striatal lysates from DAT-P2A-Flpo WT and heterozygous (Het) mice. Two independent samples per genotype are shown. Blots were cropped to show the relevant bands. Molecular weight (MW) in kDa is indicated on the right. Representative of 4 mice.(B) Quantification of protein levels relative to H3, normalized to WT. Bars represent mean ± SEM. Each dot represents the average of two striatal samples from one mouse (n = 4 mice per genotype, 2 males and 2 females, P100–120). DAT, *p = 0.0259; TH, p = 0.9639; VMAT2, p = 0.0930; unpaired t tests. (C) Extracellular DA ([DA] o ) transients evoked by single electrical pulses and recorded with fast-scan cyclic voltammetry (FCV) in different striatal sub-regions. Traces are mean ± SEM [DA] o versus time (average of 32 transients per recording site from 4 pairs of DAT-P2A-Flpo WT and Het mice, 2 male pairs and 2 female pairs, P100–120). 1, ventromedial striatum; 2, dorsomedial striatum; 3, dorsolateral striatum; 4, central striatum; 5, ventrolateral striatum; 6+7, nucleus accumbens (NAc). (D) Mean ± SEM [DA] o versus time (average of 80 transients per genotype) from the dorsal striatum (dStr) sites 1–5. (E) Mean ± SEM [DA] o versus time (average of 32 transients per genotype) from the NAc sites 6+7. (F) Mean ± SEM [DA] o of selected region- and peak-matched transients from the dStr of DAT-P2A-Flpo WT and Het mice (average of 22 transients per genotype). (G) Single exponential curve fit of the decay phase of the transients in (F). DAT-P2A-Flpo Het mice have significantly slower [DA] o re-uptake than WT mice (***p
    Figure Legend Snippet: DAT expression and function in DAT-P2A-Flpo mice (A) Representative western blot images of DAT, TH, vesicular monoamine transporter 2 (VMAT2), and histone H3 (H3) loading control from striatal lysates from DAT-P2A-Flpo WT and heterozygous (Het) mice. Two independent samples per genotype are shown. Blots were cropped to show the relevant bands. Molecular weight (MW) in kDa is indicated on the right. Representative of 4 mice.(B) Quantification of protein levels relative to H3, normalized to WT. Bars represent mean ± SEM. Each dot represents the average of two striatal samples from one mouse (n = 4 mice per genotype, 2 males and 2 females, P100–120). DAT, *p = 0.0259; TH, p = 0.9639; VMAT2, p = 0.0930; unpaired t tests. (C) Extracellular DA ([DA] o ) transients evoked by single electrical pulses and recorded with fast-scan cyclic voltammetry (FCV) in different striatal sub-regions. Traces are mean ± SEM [DA] o versus time (average of 32 transients per recording site from 4 pairs of DAT-P2A-Flpo WT and Het mice, 2 male pairs and 2 female pairs, P100–120). 1, ventromedial striatum; 2, dorsomedial striatum; 3, dorsolateral striatum; 4, central striatum; 5, ventrolateral striatum; 6+7, nucleus accumbens (NAc). (D) Mean ± SEM [DA] o versus time (average of 80 transients per genotype) from the dorsal striatum (dStr) sites 1–5. (E) Mean ± SEM [DA] o versus time (average of 32 transients per genotype) from the NAc sites 6+7. (F) Mean ± SEM [DA] o of selected region- and peak-matched transients from the dStr of DAT-P2A-Flpo WT and Het mice (average of 22 transients per genotype). (G) Single exponential curve fit of the decay phase of the transients in (F). DAT-P2A-Flpo Het mice have significantly slower [DA] o re-uptake than WT mice (***p

    Techniques Used: Expressing, Mouse Assay, Western Blot, Molecular Weight

    3) Product Images from "Generation of a DAT-Flp mouse line for intersectional genetic targeting of dopamine neuron subpopulations"

    Article Title: Generation of a DAT-Flp mouse line for intersectional genetic targeting of dopamine neuron subpopulations

    Journal: bioRxiv

    doi: 10.1101/2020.06.24.167908

    DAT expression and function in DAT-IRES-Cre mice (related to Figure 2). A) Representative western blot images of dopamine active transporter (DAT), tyrosine hydroxylase (TH), vesicular monoamine transporter 2 (VMAT2), and histone H3 (H3) loading control from striatal lysates from DAT-IRES-Cre wild-type (WT) and heterozygous (Het) mice. Two samples per genotype are shown. Blots were cropped to show the relevant bands. Molecular weight (MW) in KD is indicated on the right. B) Quantification of protein levels relative to histone H3, normalized to WT. Bars represent mean +/- SEM. Each dot represents the average of two striatal samples from one mouse (n=4 mice per genotype, 1 male and 3 females P100-120). DAT: p=0.0009, TH: p=0.0121, VMAT2: p=0.5655; unpaired t-tests. C) Fast-scan cyclic voltammetry (FCV) traces of extracellular DA ([DA] o ) evoked by single electrical pulses in different striatal sub-regions in DAT-IRES-Cre mice. Traces are mean +/- SEM [DA] o versus time (average of 27-28 transients per site from 4 pairs of DAT-IRES-Cre WT and Het mice, 1 male pair and 3 female pairs; P100-120). 1: ventromedial striatum, 2: dorsomedial striatum, 3: dorsolateral striatum, 4: central striatum, 5: ventrolateral striatum, 6-7: nucleus accumbens. D) Mean +/- SEM [DA] o versus time (average of 80 transients per genotype) from the dorsal striatum sites #1-5. E) Mean +/- SEM [DA] o versus time (average of 27-28 transients per genotype) from the nucleus accumbens sites #6-7. F) Region- and peak-matched mean +/- SEM [DA] o versus time from the dorsal striatum of DAT-IRES-Cre WT and Het mice (average of 34-36 transients per genotype). p=0.072, single-phase exponential decay curve fit; WT tau=0.346, Het tau=0.355. G-J) Behavioral performance of DAT-IRES-Cre mice in a 60-minute open-field test. For all graphs, bars represent mean +/- SEM and dots represent values for individual mice. n= 15 WT mice; n=12 Heterozygous mice, all females; age P50-90. G) Total distance traveled in 60 minutes; p=0.0099, unpaired t-test. H) Total number of rears in 60 minutes; p=0.0668, unpaired t-test, I) Total time spent in the center of open field in 60 minutes; p=0.6976, unpaired t-test. J) Total number of grooming bouts in the first 20 minutes of open field test; p=0.0258, unpaired t-test.
    Figure Legend Snippet: DAT expression and function in DAT-IRES-Cre mice (related to Figure 2). A) Representative western blot images of dopamine active transporter (DAT), tyrosine hydroxylase (TH), vesicular monoamine transporter 2 (VMAT2), and histone H3 (H3) loading control from striatal lysates from DAT-IRES-Cre wild-type (WT) and heterozygous (Het) mice. Two samples per genotype are shown. Blots were cropped to show the relevant bands. Molecular weight (MW) in KD is indicated on the right. B) Quantification of protein levels relative to histone H3, normalized to WT. Bars represent mean +/- SEM. Each dot represents the average of two striatal samples from one mouse (n=4 mice per genotype, 1 male and 3 females P100-120). DAT: p=0.0009, TH: p=0.0121, VMAT2: p=0.5655; unpaired t-tests. C) Fast-scan cyclic voltammetry (FCV) traces of extracellular DA ([DA] o ) evoked by single electrical pulses in different striatal sub-regions in DAT-IRES-Cre mice. Traces are mean +/- SEM [DA] o versus time (average of 27-28 transients per site from 4 pairs of DAT-IRES-Cre WT and Het mice, 1 male pair and 3 female pairs; P100-120). 1: ventromedial striatum, 2: dorsomedial striatum, 3: dorsolateral striatum, 4: central striatum, 5: ventrolateral striatum, 6-7: nucleus accumbens. D) Mean +/- SEM [DA] o versus time (average of 80 transients per genotype) from the dorsal striatum sites #1-5. E) Mean +/- SEM [DA] o versus time (average of 27-28 transients per genotype) from the nucleus accumbens sites #6-7. F) Region- and peak-matched mean +/- SEM [DA] o versus time from the dorsal striatum of DAT-IRES-Cre WT and Het mice (average of 34-36 transients per genotype). p=0.072, single-phase exponential decay curve fit; WT tau=0.346, Het tau=0.355. G-J) Behavioral performance of DAT-IRES-Cre mice in a 60-minute open-field test. For all graphs, bars represent mean +/- SEM and dots represent values for individual mice. n= 15 WT mice; n=12 Heterozygous mice, all females; age P50-90. G) Total distance traveled in 60 minutes; p=0.0099, unpaired t-test. H) Total number of rears in 60 minutes; p=0.0668, unpaired t-test, I) Total time spent in the center of open field in 60 minutes; p=0.6976, unpaired t-test. J) Total number of grooming bouts in the first 20 minutes of open field test; p=0.0258, unpaired t-test.

    Techniques Used: Expressing, Mouse Assay, Western Blot, Molecular Weight

    DAT expression and function in DAT-Flp mice. A) Representative western blot images of dopamine active transporter (DAT), tyrosine hydroxylase (TH), vesicular monoamine transporter 2 (VMAT2), and histone H3 (H3) loading control from striatal lysates from DAT-Flp wild-type (WT) and heterozygous (Het) mice. Two independent samples per genotype are shown. Blots were cropped to show the relevant bands. Molecular weight (MW) in KD is indicated on the right. B) Quantification of protein levels relative to histone H3, normalized to WT. Bars represent mean +/- SEM. Each dot represents the average of two striatal samples from one mouse (n=4 mice per genotype, 2 males and 2 females, P100-120). DAT: p=0.0259, TH: p=0.9639, VMAT2: p=0.0930; unpaired t-tests. C) Extracellular DA ([DA] o ) transients evoked by single electrical pulses and recorded with fast-scan cyclic voltammetry (FCV) in different striatal sub-regions. Traces are mean +/- SEM [DA] o versus time (average of 32 transients per recording site from 4 pairs of DAT-Flp WT and Het mice, 2 male pairs and 2 female pairs, P100-120). 1: ventromedial striatum, 2: dorsomedial striatum, 3: dorsolateral striatum, 4: central striatum, 5: ventrolateral striatum, 6-7: nucleus accumbens. D) Mean +/- SEM [DA] o versus time (average of 80 transients per genotype) from the dorsal striatum sites #1-5. E) Mean +/- SEM [DA] o versus time (average of 32 transients per genotype) from the nucleus accumbens sites #6-7. F) Region- and peak-matched mean +/- SEM [DA] o versus time from the dorsal striatum of DAT-Flp WT and Het mice (average of 22 transients per genotype). DAT-Flp heterozygous mice have significantly slower [DA] o re-uptake than WT mice (p
    Figure Legend Snippet: DAT expression and function in DAT-Flp mice. A) Representative western blot images of dopamine active transporter (DAT), tyrosine hydroxylase (TH), vesicular monoamine transporter 2 (VMAT2), and histone H3 (H3) loading control from striatal lysates from DAT-Flp wild-type (WT) and heterozygous (Het) mice. Two independent samples per genotype are shown. Blots were cropped to show the relevant bands. Molecular weight (MW) in KD is indicated on the right. B) Quantification of protein levels relative to histone H3, normalized to WT. Bars represent mean +/- SEM. Each dot represents the average of two striatal samples from one mouse (n=4 mice per genotype, 2 males and 2 females, P100-120). DAT: p=0.0259, TH: p=0.9639, VMAT2: p=0.0930; unpaired t-tests. C) Extracellular DA ([DA] o ) transients evoked by single electrical pulses and recorded with fast-scan cyclic voltammetry (FCV) in different striatal sub-regions. Traces are mean +/- SEM [DA] o versus time (average of 32 transients per recording site from 4 pairs of DAT-Flp WT and Het mice, 2 male pairs and 2 female pairs, P100-120). 1: ventromedial striatum, 2: dorsomedial striatum, 3: dorsolateral striatum, 4: central striatum, 5: ventrolateral striatum, 6-7: nucleus accumbens. D) Mean +/- SEM [DA] o versus time (average of 80 transients per genotype) from the dorsal striatum sites #1-5. E) Mean +/- SEM [DA] o versus time (average of 32 transients per genotype) from the nucleus accumbens sites #6-7. F) Region- and peak-matched mean +/- SEM [DA] o versus time from the dorsal striatum of DAT-Flp WT and Het mice (average of 22 transients per genotype). DAT-Flp heterozygous mice have significantly slower [DA] o re-uptake than WT mice (p

    Techniques Used: Expressing, Mouse Assay, Western Blot, Molecular Weight

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    Alomone Labs anti vmat2 antibody
    DAT expression and function in DAT-P2A-Flpo mice (A) Representative western blot images of DAT, TH, vesicular monoamine transporter 2 <t>(VMAT2),</t> and histone H3 (H3) loading control from striatal lysates from DAT-P2A-Flpo WT and heterozygous (Het) mice. Two independent samples per genotype are shown. Blots were cropped to show the relevant bands. Molecular weight (MW) in kDa is indicated on the right. Representative of 4 mice.(B) Quantification of protein levels relative to H3, normalized to WT. Bars represent mean ± SEM. Each dot represents the average of two striatal samples from one mouse (n = 4 mice per genotype, 2 males and 2 females, P100–120). DAT, *p = 0.0259; TH, p = 0.9639; VMAT2, p = 0.0930; unpaired t tests. (C) Extracellular DA ([DA] o ) transients evoked by single electrical pulses and recorded with fast-scan cyclic voltammetry (FCV) in different striatal sub-regions. Traces are mean ± SEM [DA] o versus time (average of 32 transients per recording site from 4 pairs of DAT-P2A-Flpo WT and Het mice, 2 male pairs and 2 female pairs, P100–120). 1, ventromedial striatum; 2, dorsomedial striatum; 3, dorsolateral striatum; 4, central striatum; 5, ventrolateral striatum; 6+7, nucleus accumbens (NAc). (D) Mean ± SEM [DA] o versus time (average of 80 transients per genotype) from the dorsal striatum (dStr) sites 1–5. (E) Mean ± SEM [DA] o versus time (average of 32 transients per genotype) from the NAc sites 6+7. (F) Mean ± SEM [DA] o of selected region- and peak-matched transients from the dStr of DAT-P2A-Flpo WT and Het mice (average of 22 transients per genotype). (G) Single exponential curve fit of the decay phase of the transients in (F). DAT-P2A-Flpo Het mice have significantly slower [DA] o re-uptake than WT mice (***p
    Anti Vmat2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti vmat2 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti vmat2 antibody - by Bioz Stars, 2022-11
    94/100 stars
      Buy from Supplier

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    DAT expression and function in DAT-P2A-Flpo mice (A) Representative western blot images of DAT, TH, vesicular monoamine transporter 2 (VMAT2), and histone H3 (H3) loading control from striatal lysates from DAT-P2A-Flpo WT and heterozygous (Het) mice. Two independent samples per genotype are shown. Blots were cropped to show the relevant bands. Molecular weight (MW) in kDa is indicated on the right. Representative of 4 mice.(B) Quantification of protein levels relative to H3, normalized to WT. Bars represent mean ± SEM. Each dot represents the average of two striatal samples from one mouse (n = 4 mice per genotype, 2 males and 2 females, P100–120). DAT, *p = 0.0259; TH, p = 0.9639; VMAT2, p = 0.0930; unpaired t tests. (C) Extracellular DA ([DA] o ) transients evoked by single electrical pulses and recorded with fast-scan cyclic voltammetry (FCV) in different striatal sub-regions. Traces are mean ± SEM [DA] o versus time (average of 32 transients per recording site from 4 pairs of DAT-P2A-Flpo WT and Het mice, 2 male pairs and 2 female pairs, P100–120). 1, ventromedial striatum; 2, dorsomedial striatum; 3, dorsolateral striatum; 4, central striatum; 5, ventrolateral striatum; 6+7, nucleus accumbens (NAc). (D) Mean ± SEM [DA] o versus time (average of 80 transients per genotype) from the dorsal striatum (dStr) sites 1–5. (E) Mean ± SEM [DA] o versus time (average of 32 transients per genotype) from the NAc sites 6+7. (F) Mean ± SEM [DA] o of selected region- and peak-matched transients from the dStr of DAT-P2A-Flpo WT and Het mice (average of 22 transients per genotype). (G) Single exponential curve fit of the decay phase of the transients in (F). DAT-P2A-Flpo Het mice have significantly slower [DA] o re-uptake than WT mice (***p

    Journal: Cell reports

    Article Title: Generation of a DAT-P2A-Flpo mouse line for intersectional genetic targeting of dopamine neuron subpopulations

    doi: 10.1016/j.celrep.2021.109123

    Figure Lengend Snippet: DAT expression and function in DAT-P2A-Flpo mice (A) Representative western blot images of DAT, TH, vesicular monoamine transporter 2 (VMAT2), and histone H3 (H3) loading control from striatal lysates from DAT-P2A-Flpo WT and heterozygous (Het) mice. Two independent samples per genotype are shown. Blots were cropped to show the relevant bands. Molecular weight (MW) in kDa is indicated on the right. Representative of 4 mice.(B) Quantification of protein levels relative to H3, normalized to WT. Bars represent mean ± SEM. Each dot represents the average of two striatal samples from one mouse (n = 4 mice per genotype, 2 males and 2 females, P100–120). DAT, *p = 0.0259; TH, p = 0.9639; VMAT2, p = 0.0930; unpaired t tests. (C) Extracellular DA ([DA] o ) transients evoked by single electrical pulses and recorded with fast-scan cyclic voltammetry (FCV) in different striatal sub-regions. Traces are mean ± SEM [DA] o versus time (average of 32 transients per recording site from 4 pairs of DAT-P2A-Flpo WT and Het mice, 2 male pairs and 2 female pairs, P100–120). 1, ventromedial striatum; 2, dorsomedial striatum; 3, dorsolateral striatum; 4, central striatum; 5, ventrolateral striatum; 6+7, nucleus accumbens (NAc). (D) Mean ± SEM [DA] o versus time (average of 80 transients per genotype) from the dorsal striatum (dStr) sites 1–5. (E) Mean ± SEM [DA] o versus time (average of 32 transients per genotype) from the NAc sites 6+7. (F) Mean ± SEM [DA] o of selected region- and peak-matched transients from the dStr of DAT-P2A-Flpo WT and Het mice (average of 22 transients per genotype). (G) Single exponential curve fit of the decay phase of the transients in (F). DAT-P2A-Flpo Het mice have significantly slower [DA] o re-uptake than WT mice (***p

    Article Snippet: The following primary antibodies were used for western blotting: mouse anti-tyrosine hydroxylase (TH, 1:3000, Immunostar - 22941); mouse anti-Histone-3 (1:1500, Cell Signaling - 96C10); rabbit anti-VMAT2 (1:1400, Alomone Labs - AMT-006), mouse anti-Dopamine Transporter [6V-23–23] (DAT, 1:1,400, Abcam – 128848).

    Techniques: Expressing, Mouse Assay, Western Blot, Molecular Weight