polyclonal antibody  (Alomone Labs)


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  • 94
    Name:
    Anti Serotonin Transporter SERT extracellular Antibody
    Description:
    Anti Serotonin Transporter SERT extracellular Antibody AMT 004 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot immunohistochemistry immunocytochemistry indirect flow cytometry and live cell imaging applications It has been designed to recognize SERT from human mouse and rat samples
    Catalog Number:
    AMT-004
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Indirect Flow Cytometry, Immunohistochemistry, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs polyclonal antibody
    Anti Serotonin Transporter SERT extracellular Antibody
    Anti Serotonin Transporter SERT extracellular Antibody AMT 004 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot immunohistochemistry immunocytochemistry indirect flow cytometry and live cell imaging applications It has been designed to recognize SERT from human mouse and rat samples
    https://www.bioz.com/result/polyclonal antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Serotonin transporter antagonists target tumor-initiating cells in a transgenic mouse model of breast cancer"

    Article Title: Serotonin transporter antagonists target tumor-initiating cells in a transgenic mouse model of breast cancer

    Journal: Oncotarget

    doi: 10.18632/oncotarget.10614

    Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a polyclonal antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).
    Figure Legend Snippet: Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a polyclonal antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).

    Techniques Used: Expressing, Transgenic Assay, Incubation, Blocking Assay, Staining, Labeling

    2) Product Images from "Regulatory mechanism of CCN2 production by serotonin (5-HT) via 5-HT2A and 5-HT2B receptors in chondrocytes"

    Article Title: Regulatory mechanism of CCN2 production by serotonin (5-HT) via 5-HT2A and 5-HT2B receptors in chondrocytes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0188014

    Effect of 5-HT on the gene expression and protein production of 5-HT 2 receptor in HCS-2/8 cells. (A) After HCS-2/8 cells had reached confluence, they were treated with 5-HT at a concentration of 10 μM for 24 h. Then, total RNA was isolated and real-time RT-PCR analysis was performed by using specific primers for GAPDH, TpH-1, 5-HT 2A R, 5-HT 2B R and 5-HT 2C R. The ordinate indicates the relative ratio with respect to GAPDH expression, and data represents mean and standard deviation of culture with (n = 4) or without 5-HT (n = 4). The gene expressions levels of 5-HT 2A R , 5-HT 2B R , and TpH-1 were confirmed in HCS-2/8 cells, whereas 5-HT 2C R was not detected (ND). In addition, these gene expressions were not affected by the treatment with 5-HT. (B) After HCS-2/8 cells had reached confluence, they were treated with 5-HT for 24 h. Then, Western blot analysis was performed by using antibodies recognizing the indicated proteins. The apparent molecular weights of 5-HT 2A R were 75 kDa (arrow) and 50 kDa (arrowhead). The major band of 5-HT 2B R indicated a molecular weight 55 kDa (arrow), and minor bands were found at 30 kDa (arrowheads). The major band of 5-HTT was at 53 kDa (arrow), and a minor band at 75 kDa (arrowhead). Exogenous 5-HT added had no effect on the production of these proteins. Histone H3 was used as a loading control.
    Figure Legend Snippet: Effect of 5-HT on the gene expression and protein production of 5-HT 2 receptor in HCS-2/8 cells. (A) After HCS-2/8 cells had reached confluence, they were treated with 5-HT at a concentration of 10 μM for 24 h. Then, total RNA was isolated and real-time RT-PCR analysis was performed by using specific primers for GAPDH, TpH-1, 5-HT 2A R, 5-HT 2B R and 5-HT 2C R. The ordinate indicates the relative ratio with respect to GAPDH expression, and data represents mean and standard deviation of culture with (n = 4) or without 5-HT (n = 4). The gene expressions levels of 5-HT 2A R , 5-HT 2B R , and TpH-1 were confirmed in HCS-2/8 cells, whereas 5-HT 2C R was not detected (ND). In addition, these gene expressions were not affected by the treatment with 5-HT. (B) After HCS-2/8 cells had reached confluence, they were treated with 5-HT for 24 h. Then, Western blot analysis was performed by using antibodies recognizing the indicated proteins. The apparent molecular weights of 5-HT 2A R were 75 kDa (arrow) and 50 kDa (arrowhead). The major band of 5-HT 2B R indicated a molecular weight 55 kDa (arrow), and minor bands were found at 30 kDa (arrowheads). The major band of 5-HTT was at 53 kDa (arrow), and a minor band at 75 kDa (arrowhead). Exogenous 5-HT added had no effect on the production of these proteins. Histone H3 was used as a loading control.

    Techniques Used: Expressing, Concentration Assay, Isolation, Quantitative RT-PCR, Standard Deviation, Western Blot, Molecular Weight

    Related Articles

    Affinity Purification:

    Article Title: Extracellular loops of the serotonin transporter act as a selectivity filter for drug binding
    Article Snippet: .. The anti-SERT antibody (AMT-004, raised against residues 388–400 of rat SERT, affinity-purified) was from Alomone Labs. ..

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  • 94
    Alomone Labs polyclonal antibody
    Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a <t>polyclonal</t> antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).
    Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a polyclonal antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).

    Journal: Oncotarget

    Article Title: Serotonin transporter antagonists target tumor-initiating cells in a transgenic mouse model of breast cancer

    doi: 10.18632/oncotarget.10614

    Figure Lengend Snippet: Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a polyclonal antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).

    Article Snippet: A polyclonal antibody to SERT (Alomone Labs; AMT-004), elicited by immunization of rabbits with a peptide corresponding to amino acids 388-400 in the fourth extracellular loop, was used to detect the protein in mouse mammary tumor sections.

    Techniques: Expressing, Transgenic Assay, Incubation, Blocking Assay, Staining, Labeling

    Effect of 5-HT on the gene expression and protein production of 5-HT 2 receptor in HCS-2/8 cells. (A) After HCS-2/8 cells had reached confluence, they were treated with 5-HT at a concentration of 10 μM for 24 h. Then, total RNA was isolated and real-time RT-PCR analysis was performed by using specific primers for GAPDH, TpH-1, 5-HT 2A R, 5-HT 2B R and 5-HT 2C R. The ordinate indicates the relative ratio with respect to GAPDH expression, and data represents mean and standard deviation of culture with (n = 4) or without 5-HT (n = 4). The gene expressions levels of 5-HT 2A R , 5-HT 2B R , and TpH-1 were confirmed in HCS-2/8 cells, whereas 5-HT 2C R was not detected (ND). In addition, these gene expressions were not affected by the treatment with 5-HT. (B) After HCS-2/8 cells had reached confluence, they were treated with 5-HT for 24 h. Then, Western blot analysis was performed by using antibodies recognizing the indicated proteins. The apparent molecular weights of 5-HT 2A R were 75 kDa (arrow) and 50 kDa (arrowhead). The major band of 5-HT 2B R indicated a molecular weight 55 kDa (arrow), and minor bands were found at 30 kDa (arrowheads). The major band of 5-HTT was at 53 kDa (arrow), and a minor band at 75 kDa (arrowhead). Exogenous 5-HT added had no effect on the production of these proteins. Histone H3 was used as a loading control.

    Journal: PLoS ONE

    Article Title: Regulatory mechanism of CCN2 production by serotonin (5-HT) via 5-HT2A and 5-HT2B receptors in chondrocytes

    doi: 10.1371/journal.pone.0188014

    Figure Lengend Snippet: Effect of 5-HT on the gene expression and protein production of 5-HT 2 receptor in HCS-2/8 cells. (A) After HCS-2/8 cells had reached confluence, they were treated with 5-HT at a concentration of 10 μM for 24 h. Then, total RNA was isolated and real-time RT-PCR analysis was performed by using specific primers for GAPDH, TpH-1, 5-HT 2A R, 5-HT 2B R and 5-HT 2C R. The ordinate indicates the relative ratio with respect to GAPDH expression, and data represents mean and standard deviation of culture with (n = 4) or without 5-HT (n = 4). The gene expressions levels of 5-HT 2A R , 5-HT 2B R , and TpH-1 were confirmed in HCS-2/8 cells, whereas 5-HT 2C R was not detected (ND). In addition, these gene expressions were not affected by the treatment with 5-HT. (B) After HCS-2/8 cells had reached confluence, they were treated with 5-HT for 24 h. Then, Western blot analysis was performed by using antibodies recognizing the indicated proteins. The apparent molecular weights of 5-HT 2A R were 75 kDa (arrow) and 50 kDa (arrowhead). The major band of 5-HT 2B R indicated a molecular weight 55 kDa (arrow), and minor bands were found at 30 kDa (arrowheads). The major band of 5-HTT was at 53 kDa (arrow), and a minor band at 75 kDa (arrowhead). Exogenous 5-HT added had no effect on the production of these proteins. Histone H3 was used as a loading control.

    Article Snippet: Anti-5-HT2A R and anti-CCN2 antibodies were purchased from Abcam (Cambridge, UK), and anti-5-HT transporter (5-HTT) antibodies were from Alomone Labs (Jerusalem, Israel).

    Techniques: Expressing, Concentration Assay, Isolation, Quantitative RT-PCR, Standard Deviation, Western Blot, Molecular Weight