polyclonal antibody  (Alomone Labs)


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    Alomone Labs polyclonal antibody
    Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a <t>polyclonal</t> antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).
    Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody/product/Alomone Labs
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody - by Bioz Stars, 2022-08
    93/100 stars

    Images

    1) Product Images from "Serotonin transporter antagonists target tumor-initiating cells in a transgenic mouse model of breast cancer"

    Article Title: Serotonin transporter antagonists target tumor-initiating cells in a transgenic mouse model of breast cancer

    Journal: Oncotarget

    doi: 10.18632/oncotarget.10614

    Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a polyclonal antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).
    Figure Legend Snippet: Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a polyclonal antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).

    Techniques Used: Expressing, Transgenic Assay, Incubation, Blocking Assay, Staining, Labeling

    2) Product Images from "Serotonergic system antagonists target breast tumor initiating cells and synergize with chemotherapy to shrink human breast tumor xenografts"

    Article Title: Serotonergic system antagonists target breast tumor initiating cells and synergize with chemotherapy to shrink human breast tumor xenografts

    Journal: Oncotarget

    doi: 10.18632/oncotarget.16646

    SERT is expressed in breast tumor cell lines representative of all the molecular subtypes of breast cancer Western blot performed with breast tumor cell line lysates (35 μg) and mouse brain tissue after electrophoresis in 5-15% gradient polyacrylamide gels reveal that the rabbit polyclonal antibody identifies SERT species that migrate between molecular mass markers of 63 kDa and 75 kDa, and between 48 kDa and 63 kDa. The arrowheads identify the glycosylated and non-glycosylated SERT species. Non-glycosylated SERT co-migrated with α-tubulin, the loading control, which is shown in the bottom-most panel.
    Figure Legend Snippet: SERT is expressed in breast tumor cell lines representative of all the molecular subtypes of breast cancer Western blot performed with breast tumor cell line lysates (35 μg) and mouse brain tissue after electrophoresis in 5-15% gradient polyacrylamide gels reveal that the rabbit polyclonal antibody identifies SERT species that migrate between molecular mass markers of 63 kDa and 75 kDa, and between 48 kDa and 63 kDa. The arrowheads identify the glycosylated and non-glycosylated SERT species. Non-glycosylated SERT co-migrated with α-tubulin, the loading control, which is shown in the bottom-most panel.

    Techniques Used: Western Blot, Electrophoresis

    Patient-derived breast tumor xenografts recapitulate the phenotypic heterogeneity of the primary tumor from which they were derived and express SERT (a) Six primary human breast tumor samples and companion xenografts (b) were stained with H E to reveal their histology. (c) SERT expression was detected by IHC in the PDX sections with the SERT-selective rabbit polyclonal antibody. The arrows identify tumor cells that express higher levels of SERT than the majority of the SERT-positive tumor cells in the same field. The scale bars represent 50 μm.
    Figure Legend Snippet: Patient-derived breast tumor xenografts recapitulate the phenotypic heterogeneity of the primary tumor from which they were derived and express SERT (a) Six primary human breast tumor samples and companion xenografts (b) were stained with H E to reveal their histology. (c) SERT expression was detected by IHC in the PDX sections with the SERT-selective rabbit polyclonal antibody. The arrows identify tumor cells that express higher levels of SERT than the majority of the SERT-positive tumor cells in the same field. The scale bars represent 50 μm.

    Techniques Used: Derivative Assay, Staining, Expressing, Immunohistochemistry

    3) Product Images from "Regulatory mechanism of CCN2 production by serotonin (5-HT) via 5-HT2A and 5-HT2B receptors in chondrocytes"

    Article Title: Regulatory mechanism of CCN2 production by serotonin (5-HT) via 5-HT2A and 5-HT2B receptors in chondrocytes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0188014

    Effect of 5-HT on the gene expression and protein production of 5-HT 2 receptor in HCS-2/8 cells. (A) After HCS-2/8 cells had reached confluence, they were treated with 5-HT at a concentration of 10 μM for 24 h. Then, total RNA was isolated and real-time RT-PCR analysis was performed by using specific primers for GAPDH, TpH-1, 5-HT 2A R, 5-HT 2B R and 5-HT 2C R. The ordinate indicates the relative ratio with respect to GAPDH expression, and data represents mean and standard deviation of culture with (n = 4) or without 5-HT (n = 4). The gene expressions levels of 5-HT 2A R , 5-HT 2B R , and TpH-1 were confirmed in HCS-2/8 cells, whereas 5-HT 2C R was not detected (ND). In addition, these gene expressions were not affected by the treatment with 5-HT. (B) After HCS-2/8 cells had reached confluence, they were treated with 5-HT for 24 h. Then, Western blot analysis was performed by using antibodies recognizing the indicated proteins. The apparent molecular weights of 5-HT 2A R were 75 kDa (arrow) and 50 kDa (arrowhead). The major band of 5-HT 2B R indicated a molecular weight 55 kDa (arrow), and minor bands were found at 30 kDa (arrowheads). The major band of 5-HTT was at 53 kDa (arrow), and a minor band at 75 kDa (arrowhead). Exogenous 5-HT added had no effect on the production of these proteins. Histone H3 was used as a loading control.
    Figure Legend Snippet: Effect of 5-HT on the gene expression and protein production of 5-HT 2 receptor in HCS-2/8 cells. (A) After HCS-2/8 cells had reached confluence, they were treated with 5-HT at a concentration of 10 μM for 24 h. Then, total RNA was isolated and real-time RT-PCR analysis was performed by using specific primers for GAPDH, TpH-1, 5-HT 2A R, 5-HT 2B R and 5-HT 2C R. The ordinate indicates the relative ratio with respect to GAPDH expression, and data represents mean and standard deviation of culture with (n = 4) or without 5-HT (n = 4). The gene expressions levels of 5-HT 2A R , 5-HT 2B R , and TpH-1 were confirmed in HCS-2/8 cells, whereas 5-HT 2C R was not detected (ND). In addition, these gene expressions were not affected by the treatment with 5-HT. (B) After HCS-2/8 cells had reached confluence, they were treated with 5-HT for 24 h. Then, Western blot analysis was performed by using antibodies recognizing the indicated proteins. The apparent molecular weights of 5-HT 2A R were 75 kDa (arrow) and 50 kDa (arrowhead). The major band of 5-HT 2B R indicated a molecular weight 55 kDa (arrow), and minor bands were found at 30 kDa (arrowheads). The major band of 5-HTT was at 53 kDa (arrow), and a minor band at 75 kDa (arrowhead). Exogenous 5-HT added had no effect on the production of these proteins. Histone H3 was used as a loading control.

    Techniques Used: Expressing, Concentration Assay, Isolation, Quantitative RT-PCR, Standard Deviation, Western Blot, Molecular Weight

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    Alomone Labs polyclonal antibody
    Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a <t>polyclonal</t> antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).
    Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody - by Bioz Stars, 2022-08
    93/100 stars
      Buy from Supplier

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    Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a polyclonal antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).

    Journal: Oncotarget

    Article Title: Serotonin transporter antagonists target tumor-initiating cells in a transgenic mouse model of breast cancer

    doi: 10.18632/oncotarget.10614

    Figure Lengend Snippet: Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a polyclonal antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).

    Article Snippet: A polyclonal antibody to SERT (Alomone Labs; AMT-004), elicited by immunization of rabbits with a peptide corresponding to amino acids 388-400 in the fourth extracellular loop, was used to detect the protein in mouse mammary tumor sections.

    Techniques: Expressing, Transgenic Assay, Incubation, Blocking Assay, Staining, Labeling

    SERT is expressed in breast tumor cell lines representative of all the molecular subtypes of breast cancer Western blot performed with breast tumor cell line lysates (35 μg) and mouse brain tissue after electrophoresis in 5-15% gradient polyacrylamide gels reveal that the rabbit polyclonal antibody identifies SERT species that migrate between molecular mass markers of 63 kDa and 75 kDa, and between 48 kDa and 63 kDa. The arrowheads identify the glycosylated and non-glycosylated SERT species. Non-glycosylated SERT co-migrated with α-tubulin, the loading control, which is shown in the bottom-most panel.

    Journal: Oncotarget

    Article Title: Serotonergic system antagonists target breast tumor initiating cells and synergize with chemotherapy to shrink human breast tumor xenografts

    doi: 10.18632/oncotarget.16646

    Figure Lengend Snippet: SERT is expressed in breast tumor cell lines representative of all the molecular subtypes of breast cancer Western blot performed with breast tumor cell line lysates (35 μg) and mouse brain tissue after electrophoresis in 5-15% gradient polyacrylamide gels reveal that the rabbit polyclonal antibody identifies SERT species that migrate between molecular mass markers of 63 kDa and 75 kDa, and between 48 kDa and 63 kDa. The arrowheads identify the glycosylated and non-glycosylated SERT species. Non-glycosylated SERT co-migrated with α-tubulin, the loading control, which is shown in the bottom-most panel.

    Article Snippet: To identify human SERT in tumor cell lysates we used a polyclonal antibody to SERT (Alomone Labs; Jerusalem, Israel), elicited by immunization of rabbits with a peptide corresponding to amino acids 388-400.

    Techniques: Western Blot, Electrophoresis

    Patient-derived breast tumor xenografts recapitulate the phenotypic heterogeneity of the primary tumor from which they were derived and express SERT (a) Six primary human breast tumor samples and companion xenografts (b) were stained with H E to reveal their histology. (c) SERT expression was detected by IHC in the PDX sections with the SERT-selective rabbit polyclonal antibody. The arrows identify tumor cells that express higher levels of SERT than the majority of the SERT-positive tumor cells in the same field. The scale bars represent 50 μm.

    Journal: Oncotarget

    Article Title: Serotonergic system antagonists target breast tumor initiating cells and synergize with chemotherapy to shrink human breast tumor xenografts

    doi: 10.18632/oncotarget.16646

    Figure Lengend Snippet: Patient-derived breast tumor xenografts recapitulate the phenotypic heterogeneity of the primary tumor from which they were derived and express SERT (a) Six primary human breast tumor samples and companion xenografts (b) were stained with H E to reveal their histology. (c) SERT expression was detected by IHC in the PDX sections with the SERT-selective rabbit polyclonal antibody. The arrows identify tumor cells that express higher levels of SERT than the majority of the SERT-positive tumor cells in the same field. The scale bars represent 50 μm.

    Article Snippet: To identify human SERT in tumor cell lysates we used a polyclonal antibody to SERT (Alomone Labs; Jerusalem, Israel), elicited by immunization of rabbits with a peptide corresponding to amino acids 388-400.

    Techniques: Derivative Assay, Staining, Expressing, Immunohistochemistry

    Effect of 5-HT on the gene expression and protein production of 5-HT 2 receptor in HCS-2/8 cells. (A) After HCS-2/8 cells had reached confluence, they were treated with 5-HT at a concentration of 10 μM for 24 h. Then, total RNA was isolated and real-time RT-PCR analysis was performed by using specific primers for GAPDH, TpH-1, 5-HT 2A R, 5-HT 2B R and 5-HT 2C R. The ordinate indicates the relative ratio with respect to GAPDH expression, and data represents mean and standard deviation of culture with (n = 4) or without 5-HT (n = 4). The gene expressions levels of 5-HT 2A R , 5-HT 2B R , and TpH-1 were confirmed in HCS-2/8 cells, whereas 5-HT 2C R was not detected (ND). In addition, these gene expressions were not affected by the treatment with 5-HT. (B) After HCS-2/8 cells had reached confluence, they were treated with 5-HT for 24 h. Then, Western blot analysis was performed by using antibodies recognizing the indicated proteins. The apparent molecular weights of 5-HT 2A R were 75 kDa (arrow) and 50 kDa (arrowhead). The major band of 5-HT 2B R indicated a molecular weight 55 kDa (arrow), and minor bands were found at 30 kDa (arrowheads). The major band of 5-HTT was at 53 kDa (arrow), and a minor band at 75 kDa (arrowhead). Exogenous 5-HT added had no effect on the production of these proteins. Histone H3 was used as a loading control.

    Journal: PLoS ONE

    Article Title: Regulatory mechanism of CCN2 production by serotonin (5-HT) via 5-HT2A and 5-HT2B receptors in chondrocytes

    doi: 10.1371/journal.pone.0188014

    Figure Lengend Snippet: Effect of 5-HT on the gene expression and protein production of 5-HT 2 receptor in HCS-2/8 cells. (A) After HCS-2/8 cells had reached confluence, they were treated with 5-HT at a concentration of 10 μM for 24 h. Then, total RNA was isolated and real-time RT-PCR analysis was performed by using specific primers for GAPDH, TpH-1, 5-HT 2A R, 5-HT 2B R and 5-HT 2C R. The ordinate indicates the relative ratio with respect to GAPDH expression, and data represents mean and standard deviation of culture with (n = 4) or without 5-HT (n = 4). The gene expressions levels of 5-HT 2A R , 5-HT 2B R , and TpH-1 were confirmed in HCS-2/8 cells, whereas 5-HT 2C R was not detected (ND). In addition, these gene expressions were not affected by the treatment with 5-HT. (B) After HCS-2/8 cells had reached confluence, they were treated with 5-HT for 24 h. Then, Western blot analysis was performed by using antibodies recognizing the indicated proteins. The apparent molecular weights of 5-HT 2A R were 75 kDa (arrow) and 50 kDa (arrowhead). The major band of 5-HT 2B R indicated a molecular weight 55 kDa (arrow), and minor bands were found at 30 kDa (arrowheads). The major band of 5-HTT was at 53 kDa (arrow), and a minor band at 75 kDa (arrowhead). Exogenous 5-HT added had no effect on the production of these proteins. Histone H3 was used as a loading control.

    Article Snippet: Anti-5-HT2A R and anti-CCN2 antibodies were purchased from Abcam (Cambridge, UK), and anti-5-HT transporter (5-HTT) antibodies were from Alomone Labs (Jerusalem, Israel).

    Techniques: Expressing, Concentration Assay, Isolation, Quantitative RT-PCR, Standard Deviation, Western Blot, Molecular Weight