polyclonal antibody  (Alomone Labs)


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    Alomone Labs polyclonal antibody
    Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a <t>polyclonal</t> antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).
    Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody - by Bioz Stars, 2022-01
    94/100 stars

    Images

    1) Product Images from "Serotonin transporter antagonists target tumor-initiating cells in a transgenic mouse model of breast cancer"

    Article Title: Serotonin transporter antagonists target tumor-initiating cells in a transgenic mouse model of breast cancer

    Journal: Oncotarget

    doi: 10.18632/oncotarget.10614

    Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a polyclonal antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).
    Figure Legend Snippet: Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a polyclonal antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).

    Techniques Used: Expressing, Transgenic Assay, Incubation, Blocking Assay, Staining, Labeling

    2) Product Images from "Regulatory mechanism of CCN2 production by serotonin (5-HT) via 5-HT2A and 5-HT2B receptors in chondrocytes"

    Article Title: Regulatory mechanism of CCN2 production by serotonin (5-HT) via 5-HT2A and 5-HT2B receptors in chondrocytes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0188014

    Effect of 5-HT on the gene expression and protein production of 5-HT 2 receptor in HCS-2/8 cells. (A) After HCS-2/8 cells had reached confluence, they were treated with 5-HT at a concentration of 10 μM for 24 h. Then, total RNA was isolated and real-time RT-PCR analysis was performed by using specific primers for GAPDH, TpH-1, 5-HT 2A R, 5-HT 2B R and 5-HT 2C R. The ordinate indicates the relative ratio with respect to GAPDH expression, and data represents mean and standard deviation of culture with (n = 4) or without 5-HT (n = 4). The gene expressions levels of 5-HT 2A R , 5-HT 2B R , and TpH-1 were confirmed in HCS-2/8 cells, whereas 5-HT 2C R was not detected (ND). In addition, these gene expressions were not affected by the treatment with 5-HT. (B) After HCS-2/8 cells had reached confluence, they were treated with 5-HT for 24 h. Then, Western blot analysis was performed by using antibodies recognizing the indicated proteins. The apparent molecular weights of 5-HT 2A R were 75 kDa (arrow) and 50 kDa (arrowhead). The major band of 5-HT 2B R indicated a molecular weight 55 kDa (arrow), and minor bands were found at 30 kDa (arrowheads). The major band of 5-HTT was at 53 kDa (arrow), and a minor band at 75 kDa (arrowhead). Exogenous 5-HT added had no effect on the production of these proteins. Histone H3 was used as a loading control.
    Figure Legend Snippet: Effect of 5-HT on the gene expression and protein production of 5-HT 2 receptor in HCS-2/8 cells. (A) After HCS-2/8 cells had reached confluence, they were treated with 5-HT at a concentration of 10 μM for 24 h. Then, total RNA was isolated and real-time RT-PCR analysis was performed by using specific primers for GAPDH, TpH-1, 5-HT 2A R, 5-HT 2B R and 5-HT 2C R. The ordinate indicates the relative ratio with respect to GAPDH expression, and data represents mean and standard deviation of culture with (n = 4) or without 5-HT (n = 4). The gene expressions levels of 5-HT 2A R , 5-HT 2B R , and TpH-1 were confirmed in HCS-2/8 cells, whereas 5-HT 2C R was not detected (ND). In addition, these gene expressions were not affected by the treatment with 5-HT. (B) After HCS-2/8 cells had reached confluence, they were treated with 5-HT for 24 h. Then, Western blot analysis was performed by using antibodies recognizing the indicated proteins. The apparent molecular weights of 5-HT 2A R were 75 kDa (arrow) and 50 kDa (arrowhead). The major band of 5-HT 2B R indicated a molecular weight 55 kDa (arrow), and minor bands were found at 30 kDa (arrowheads). The major band of 5-HTT was at 53 kDa (arrow), and a minor band at 75 kDa (arrowhead). Exogenous 5-HT added had no effect on the production of these proteins. Histone H3 was used as a loading control.

    Techniques Used: Expressing, Concentration Assay, Isolation, Quantitative RT-PCR, Standard Deviation, Western Blot, Molecular Weight

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    Alomone Labs polyclonal antibody
    Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a <t>polyclonal</t> antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).
    Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal antibody - by Bioz Stars, 2022-01
    94/100 stars
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    Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a polyclonal antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).

    Journal: Oncotarget

    Article Title: Serotonin transporter antagonists target tumor-initiating cells in a transgenic mouse model of breast cancer

    doi: 10.18632/oncotarget.10614

    Figure Lengend Snippet: Expression of SERT, TPH1 and 5-HT in 3 independent tumors from the MMTV-Neu transgenic strain ( A ) Independent tumor sections were incubated with a polyclonal antibody to SERT without or with a blocking peptide, the antigen used to elicit antibody production in rabbits. ( B ) Independent tumor sections were incubated with an antibody to TPH1. ( C ) Independent tumor sections stained with an antibody that specifically binds to 5-HT. Primary antibodies to SERT (red), TPH1 (red) and 5-HT (green) were used in combination with fluor-labeled secondary antibodies as described in Materials and Methods. The scale bar represents 50 micrometers (μm).

    Article Snippet: A polyclonal antibody to SERT (Alomone Labs; AMT-004), elicited by immunization of rabbits with a peptide corresponding to amino acids 388-400 in the fourth extracellular loop, was used to detect the protein in mouse mammary tumor sections.

    Techniques: Expressing, Transgenic Assay, Incubation, Blocking Assay, Staining, Labeling

    Effect of 5-HT on the gene expression and protein production of 5-HT 2 receptor in HCS-2/8 cells. (A) After HCS-2/8 cells had reached confluence, they were treated with 5-HT at a concentration of 10 μM for 24 h. Then, total RNA was isolated and real-time RT-PCR analysis was performed by using specific primers for GAPDH, TpH-1, 5-HT 2A R, 5-HT 2B R and 5-HT 2C R. The ordinate indicates the relative ratio with respect to GAPDH expression, and data represents mean and standard deviation of culture with (n = 4) or without 5-HT (n = 4). The gene expressions levels of 5-HT 2A R , 5-HT 2B R , and TpH-1 were confirmed in HCS-2/8 cells, whereas 5-HT 2C R was not detected (ND). In addition, these gene expressions were not affected by the treatment with 5-HT. (B) After HCS-2/8 cells had reached confluence, they were treated with 5-HT for 24 h. Then, Western blot analysis was performed by using antibodies recognizing the indicated proteins. The apparent molecular weights of 5-HT 2A R were 75 kDa (arrow) and 50 kDa (arrowhead). The major band of 5-HT 2B R indicated a molecular weight 55 kDa (arrow), and minor bands were found at 30 kDa (arrowheads). The major band of 5-HTT was at 53 kDa (arrow), and a minor band at 75 kDa (arrowhead). Exogenous 5-HT added had no effect on the production of these proteins. Histone H3 was used as a loading control.

    Journal: PLoS ONE

    Article Title: Regulatory mechanism of CCN2 production by serotonin (5-HT) via 5-HT2A and 5-HT2B receptors in chondrocytes

    doi: 10.1371/journal.pone.0188014

    Figure Lengend Snippet: Effect of 5-HT on the gene expression and protein production of 5-HT 2 receptor in HCS-2/8 cells. (A) After HCS-2/8 cells had reached confluence, they were treated with 5-HT at a concentration of 10 μM for 24 h. Then, total RNA was isolated and real-time RT-PCR analysis was performed by using specific primers for GAPDH, TpH-1, 5-HT 2A R, 5-HT 2B R and 5-HT 2C R. The ordinate indicates the relative ratio with respect to GAPDH expression, and data represents mean and standard deviation of culture with (n = 4) or without 5-HT (n = 4). The gene expressions levels of 5-HT 2A R , 5-HT 2B R , and TpH-1 were confirmed in HCS-2/8 cells, whereas 5-HT 2C R was not detected (ND). In addition, these gene expressions were not affected by the treatment with 5-HT. (B) After HCS-2/8 cells had reached confluence, they were treated with 5-HT for 24 h. Then, Western blot analysis was performed by using antibodies recognizing the indicated proteins. The apparent molecular weights of 5-HT 2A R were 75 kDa (arrow) and 50 kDa (arrowhead). The major band of 5-HT 2B R indicated a molecular weight 55 kDa (arrow), and minor bands were found at 30 kDa (arrowheads). The major band of 5-HTT was at 53 kDa (arrow), and a minor band at 75 kDa (arrowhead). Exogenous 5-HT added had no effect on the production of these proteins. Histone H3 was used as a loading control.

    Article Snippet: Anti-5-HT2A R and anti-CCN2 antibodies were purchased from Abcam (Cambridge, UK), and anti-5-HT transporter (5-HTT) antibodies were from Alomone Labs (Jerusalem, Israel).

    Techniques: Expressing, Concentration Assay, Isolation, Quantitative RT-PCR, Standard Deviation, Western Blot, Molecular Weight