chloride channel clc k  (Alomone Labs)


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    • 94
    Name:
    Anti CLC K Antibody
    Description:
    Anti CLC K Antibody ACL 004 is an antibody directed against an epitope of rat CLC K2 The antibody can be used in western blot and immunohistochemistry applications It has been designed to recognize CLC K channel from rat mouse and human samples The antibody recognizes both CLC K1 and CLC K2 isoforms
    Catalog Number:
    ACL-004
    Price:
    495.0
    Category:
    Primary Antibody
    Applications:
    Immunofluorescence, Immunohistochemistry, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    50 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs chloride channel clc k
    Anti CLC K Antibody
    Anti CLC K Antibody ACL 004 is an antibody directed against an epitope of rat CLC K2 The antibody can be used in western blot and immunohistochemistry applications It has been designed to recognize CLC K channel from rat mouse and human samples The antibody recognizes both CLC K1 and CLC K2 isoforms
    https://www.bioz.com/result/chloride channel clc k/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chloride channel clc k - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Centrosome amplification disrupts renal development and causes cystogenesis"

    Article Title: Centrosome amplification disrupts renal development and causes cystogenesis

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.201710019

    CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and CLC-K–positive distal tubules, but absent in LTL-positive proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P
    Figure Legend Snippet: CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and CLC-K–positive distal tubules, but absent in LTL-positive proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P

    Techniques Used: Mouse Assay, Staining, Isolation, Expressing, Immunostaining, Two Tailed Test

    Related Articles

    Incubation:

    Article Title: Angiotensin II increases activity of the ClC-K2 Cl− channel in collecting duct intercalated cells by stimulating production of reactive oxygen species
    Article Snippet: .. Nitrocellulose membranes were incubated with primary anti-ClC-K antibodies (rabbit polyclonal, 1:1000 Alomone Labs, Cat. # ACL-004) overnight at +4 °C. ..

    Article Title: Angiotensin II increases activity of the ClC-K2 Cl− channel in collecting duct intercalated cells by stimulating production of reactive oxygen species
    Article Snippet: .. Subsequently, nonspecific staining was blocked with 10% rabbit serum for 30 min and samples were incubated with anti-CLC-K antibody (1:500 dilution; Alomone, Cat # ACL-004) conjugated with goat anti-rabbit IgG labeled with Alexa Fluor 594 for 2 h at 37 °C in the dark. ..

    SDS Page:

    Article Title: FKBP8 Enhances Protein Stability of the CLC-1 Chloride Channel at the Plasma Membrane
    Article Snippet: .. Samples were then separated by 7.5–10% SDS-PAGE, electrophoretically transferred to nitrocellulose membranes, and detected using rabbit anti-Aha1 (1:2500; Thermo Scientific, Waltham, MA, USA), mouse anti-cadherin (1:1000; Abcam, Cambridge, MA, USA), mouse anti-calnexin (1:1000; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-CLC-1 (1:1000; Alomone, Jerusalem, Israel), rabbit anti-Flag (1:5000; Sigma-Aldrich), rabbit anti-FKBP8 (1:5000; GeneTex, Hsinchu, Taiwan), rabbit anti-GAPDH (1:8000; GeneTex), rat anti-HA (1:5000; Roche Applied Science), rabbit anti-GFP (1:5000; Abcam), mouse anti-GM130 (1:1000; BD Biosciences, San Jose, CA, USA), rabbit anti-HOP (1:10,000; Abcam), rabbit anti-Hsc70 (1:750; Abcam), rabbit anti- Hsp90β (1:500; Abcam), mouse anti-Myc (clone 9E10), or mouse anti-Na+ -K+-ATPase (1:1000; Thermo Scientific) antibodies. ..

    other:

    Article Title: Gi Protein Modulation of the Potassium Channel TASK-2 Mediates Vesicle Osmotic Swelling to Facilitate the Fusion of Aquaporin-2 Water Channel Containing Vesicles
    Article Snippet: Antibody anti-ClC-K (#ACL004) was from Alomone Labs (Jerusalem, Israel).

    In Vitro:

    Article Title: DHHC7-mediated palmitoylation of the accessory protein barttin critically regulates the functions of ClC-K chloride channels
    Article Snippet: .. For detection of the ClC-K channels, we applied rabbit polyclonal antibody from Alomone Labs, which has long been accepted as a highly specific and fairly unambiguous tool for visualization of the ClC-K channel both in vitro and in vivo ( , ). ..

    In Vivo:

    Article Title: DHHC7-mediated palmitoylation of the accessory protein barttin critically regulates the functions of ClC-K chloride channels
    Article Snippet: .. For detection of the ClC-K channels, we applied rabbit polyclonal antibody from Alomone Labs, which has long been accepted as a highly specific and fairly unambiguous tool for visualization of the ClC-K channel both in vitro and in vivo ( , ). ..

    Marker:

    Article Title: Centrosome amplification disrupts renal development and causes cystogenesis
    Article Snippet: .. For postnatal kidneys, samples were costained with nephron segment markers including LTL (marker of proximal tubules; Vector Laboratories), antibodies against the chloride channel CLC-K (marker of distal tubules; Alomone Labs; gift of F. Chen, Washington University, St. Louis, MO), and DBA (marker of collecting ducts; Vector Laboratories). ..

    Staining:

    Article Title: Angiotensin II increases activity of the ClC-K2 Cl− channel in collecting duct intercalated cells by stimulating production of reactive oxygen species
    Article Snippet: .. Subsequently, nonspecific staining was blocked with 10% rabbit serum for 30 min and samples were incubated with anti-CLC-K antibody (1:500 dilution; Alomone, Cat # ACL-004) conjugated with goat anti-rabbit IgG labeled with Alexa Fluor 594 for 2 h at 37 °C in the dark. ..

    Labeling:

    Article Title: Angiotensin II increases activity of the ClC-K2 Cl− channel in collecting duct intercalated cells by stimulating production of reactive oxygen species
    Article Snippet: .. Subsequently, nonspecific staining was blocked with 10% rabbit serum for 30 min and samples were incubated with anti-CLC-K antibody (1:500 dilution; Alomone, Cat # ACL-004) conjugated with goat anti-rabbit IgG labeled with Alexa Fluor 594 for 2 h at 37 °C in the dark. ..

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    • chloride channel clc k  (Alomone Labs)
    94
    Alomone Labs chloride channel clc k
    CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and <t>CLC-K–positive</t> distal tubules, but absent in <t>LTL-positive</t> proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P
    Chloride Channel Clc K, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chloride channel clc k/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chloride channel clc k - by Bioz Stars, 2021-09
    94/100 stars
    		  Buy from Supplier
    p2x7 r antibodies  (Alomone Labs)
    94
    Alomone Labs p2x7 r antibodies
    CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and <t>CLC-K–positive</t> distal tubules, but absent in <t>LTL-positive</t> proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P
    P2x7 R Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2x7 r antibodies/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2x7 r antibodies - by Bioz Stars, 2021-09
    94/100 stars
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    Image Search Results


    CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and CLC-K–positive distal tubules, but absent in LTL-positive proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P

    Journal: The Journal of Cell Biology

    Article Title: Centrosome amplification disrupts renal development and causes cystogenesis

    doi: 10.1083/jcb.201710019

    Figure Lengend Snippet: CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and CLC-K–positive distal tubules, but absent in LTL-positive proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P

    Article Snippet: For postnatal kidneys, samples were costained with nephron segment markers including LTL (marker of proximal tubules; Vector Laboratories), antibodies against the chloride channel CLC-K (marker of distal tubules; Alomone Labs; gift of F. Chen, Washington University, St. Louis, MO), and DBA (marker of collecting ducts; Vector Laboratories).

    Techniques: Mouse Assay, Staining, Isolation, Expressing, Immunostaining, Two Tailed Test