chloride channel clc k  (Alomone Labs)


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    Alomone Labs chloride channel clc k
    CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and <t>CLC-K–positive</t> distal tubules, but absent in <t>LTL-positive</t> proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P
    Chloride Channel Clc K, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chloride channel clc k/product/Alomone Labs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    chloride channel clc k - by Bioz Stars, 2022-08
    93/100 stars

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    1) Product Images from "Centrosome amplification disrupts renal development and causes cystogenesis"

    Article Title: Centrosome amplification disrupts renal development and causes cystogenesis

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.201710019

    CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and CLC-K–positive distal tubules, but absent in LTL-positive proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P
    Figure Legend Snippet: CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and CLC-K–positive distal tubules, but absent in LTL-positive proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P

    Techniques Used: Mouse Assay, Staining, Isolation, Expressing, Immunostaining, Two Tailed Test

    2) Product Images from "Centrosome amplification disrupts renal development and causes cystogenesis"

    Article Title: Centrosome amplification disrupts renal development and causes cystogenesis

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.201710019

    CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and CLC-K–positive distal tubules, but absent in LTL-positive proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P
    Figure Legend Snippet: CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and CLC-K–positive distal tubules, but absent in LTL-positive proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P

    Techniques Used: Mouse Assay, Staining, Isolation, Expressing, Immunostaining, Two Tailed Test

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    • chloride channel clc k  (Alomone Labs)
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    Alomone Labs chloride channel clc k
    CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and <t>CLC-K–positive</t> distal tubules, but absent in <t>LTL-positive</t> proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P
    Chloride Channel Clc K, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chloride channel clc k/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chloride channel clc k - by Bioz Stars, 2022-08
    93/100 stars
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    p2x7r intracellular epitope  (Alomone Labs)
    94
    Alomone Labs p2x7r intracellular epitope
    CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and <t>CLC-K–positive</t> distal tubules, but absent in <t>LTL-positive</t> proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P
    P2x7r Intracellular Epitope, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2x7r intracellular epitope/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2x7r intracellular epitope - by Bioz Stars, 2022-08
    94/100 stars
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    p2x7 r antibodies  (Alomone Labs)
    94
    Alomone Labs p2x7 r antibodies
    CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and <t>CLC-K–positive</t> distal tubules, but absent in <t>LTL-positive</t> proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P
    P2x7 R Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2x7 r antibodies/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p2x7 r antibodies - by Bioz Stars, 2022-08
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    CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and CLC-K–positive distal tubules, but absent in LTL-positive proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P

    Journal: The Journal of Cell Biology

    Article Title: Centrosome amplification disrupts renal development and causes cystogenesis

    doi: 10.1083/jcb.201710019

    Figure Lengend Snippet: CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and CLC-K–positive distal tubules, but absent in LTL-positive proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P

    Article Snippet: For postnatal kidneys, samples were costained with nephron segment markers including LTL (marker of proximal tubules; Vector Laboratories), antibodies against the chloride channel CLC-K (marker of distal tubules; Alomone Labs; gift of F. Chen, Washington University, St. Louis, MO), and DBA (marker of collecting ducts; Vector Laboratories).

    Techniques: Mouse Assay, Staining, Isolation, Expressing, Immunostaining, Two Tailed Test

    CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and CLC-K–positive distal tubules, but absent in LTL-positive proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P

    Journal: The Journal of Cell Biology

    Article Title: Centrosome amplification disrupts renal development and causes cystogenesis

    doi: 10.1083/jcb.201710019

    Figure Lengend Snippet: CA in adult mice sensitizes kidneys to renal injury. (A) H E staining of control and Ksp-Plk4 kidneys isolated at P7 and P30. No prominent cysts were evident at these stages. (B) Kidney samples from P30 Ksp-Plk4 mice immunostained with anti-mCherry to identify cells expressing mChPlk4. There was robust expression of Plk4 in DBA-positive collecting ducts and CLC-K–positive distal tubules, but absent in LTL-positive proximal tubules, consistent with Ksp-Cre–mediated expression. Nuclei were stained with DAPI. (C) Immunostaining of P30 control and Ksp-Plk4 kidneys to mark centrosomes (γ-tubulin), cilia (acetylated α-tubulin), collecting ducts (DBA), and DNA (DAPI). In contrast to the normal centrosome number in control kidneys, CA is evident in Ksp-Plk4. White box denotes regions shown in insets. Arrowheads point to cells with multiple centrosomes and cilia. Bars (insets), 2 µm. (D) Quantification of CA in P30 WT and Ksp-Plk4 mice. n = 747 cells (WT) and 562 cells (Ksp-Plk4). (E) Quantification of the percentage of cells expressing zero, one, or more than one primary cilium in P30 Ksp-Plk4 mice containing excess centrosomes compared with cells with normal centrosome number. CA caused an increase in the fraction of superciliated cells. n = 274 cells (WT) and 172 cells (Ksp-Plk4). Statistical significance was determined using a two-tailed unpaired t test (*, P

    Article Snippet: For postnatal kidneys, samples were costained with nephron segment markers including LTL (marker of proximal tubules; Vector Laboratories), antibodies against the chloride channel CLC-K (marker of distal tubules; Alomone Labs; gift of F. Chen, Washington University, St. Louis, MO), and DBA (marker of collecting ducts; Vector Laboratories).

    Techniques: Mouse Assay, Staining, Isolation, Expressing, Immunostaining, Two Tailed Test