rabbit anti dat antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti dat antibody
    Expression of dopamine transporter <t>(DAT),</t> norepinephrine transporter <t>(NET),</t> and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.
    Rabbit Anti Dat Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti dat antibody/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti dat antibody - by Bioz Stars, 2022-01
    91/100 stars

    Images

    1) Product Images from "Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway"

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

    Journal: ASN NEURO

    doi: 10.1177/1759091418775562

    Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.
    Figure Legend Snippet: Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Techniques Used: Expressing, Double Immunostaining, Staining

    2) Product Images from "Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway"

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

    Journal: ASN NEURO

    doi: 10.1177/1759091418775562

    Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.
    Figure Legend Snippet: Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Techniques Used: Expressing, Double Immunostaining, Staining

    3) Product Images from "Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway"

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

    Journal: ASN NEURO

    doi: 10.1177/1759091418775562

    Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.
    Figure Legend Snippet: Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Techniques Used: Expressing, Double Immunostaining, Staining

    Effects of the simultaneous inhibition of three Na + -dependent monoamine transporters (DAT, NET, and SERT) on dopamine (DA)-induced increases in pentose-phosphate pathway (PPP) flux in cortical (a) and striatal (b) astroglia. Values are the mean ± SD of four flasks. * p
    Figure Legend Snippet: Effects of the simultaneous inhibition of three Na + -dependent monoamine transporters (DAT, NET, and SERT) on dopamine (DA)-induced increases in pentose-phosphate pathway (PPP) flux in cortical (a) and striatal (b) astroglia. Values are the mean ± SD of four flasks. * p

    Techniques Used: Inhibition

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    Alomone Labs rabbit anti dat antibody
    Expression of dopamine transporter <t>(DAT),</t> norepinephrine transporter <t>(NET),</t> and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.
    Rabbit Anti Dat Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti dat antibody/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti dat antibody - by Bioz Stars, 2022-01
    91/100 stars
      Buy from Supplier

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    Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Journal: ASN NEURO

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

    doi: 10.1177/1759091418775562

    Figure Lengend Snippet: Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Article Snippet: Chemicals Chemicals and materials were obtained from the following sources: 2-deoxy-D-[1-14 C]glucose ([14 C]deoxyglucose; specific activity, 2.13 GBq/mmol), Insta-Fluor Plus, and hyamine hydroxide 10-X were obtained from Perkin-Elmer Life Sciences (Boston, MA, USA); D-[1-14 C]glucose (specific activity, 2.035 GBq/mmol) and D-[6-14 C]glucose (specific activity, 2.035 GBq/mmol) were obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA); normal goat serum was obtained from Jackson ImmunoResearch (West Grove, PA, USA); rabbit anti-DAT antibody and rabbit anti-NET were obtained from Alomone Labs (Jerusalem, Israel); mouse anti-SERT antibody was obtained from Chemicon International (Temecula, CA, USA); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) was obtained from Sigma (St. Louis, MO, USA); rabbit anti-GFAP antibody was obtained from DAKO (Glostrup, Denmark); rhodamine-conjugated goat anti-mouse (for GFAP), anti-rabbit (for GFAP) IgG antibody, and fluorescein-isothiocyanate-conjugated goat anti-rabbit (for DAT and NET) and anti-mouse (for SERT) antibody were obtained from Santa Cruz Biochemistry (Delaware, CA, USA); Dulbecco’s modified Eagle media (DMEM) with or without glucose, penicillin, and streptomycin were obtained from Life Technologies (Grand Island, NY, USA); defined fetal bovine serum was obtained from HyClone Laboratories (Logan, UT, USA); and 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) and monochlorobimane (MCB) were obtained from Molecular Probe Inc. (Eugene, OR, USA); and alamarBlue was obtained from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Expressing, Double Immunostaining, Staining

    Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Journal: ASN NEURO

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

    doi: 10.1177/1759091418775562

    Figure Lengend Snippet: Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Article Snippet: Chemicals and materials were obtained from the following sources: 2-deoxy-D-[1-14 C]glucose ([14 C]deoxyglucose; specific activity, 2.13 GBq/mmol), Insta-Fluor Plus, and hyamine hydroxide 10-X were obtained from Perkin-Elmer Life Sciences (Boston, MA, USA); D-[1-14 C]glucose (specific activity, 2.035 GBq/mmol) and D-[6-14 C]glucose (specific activity, 2.035 GBq/mmol) were obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA); normal goat serum was obtained from Jackson ImmunoResearch (West Grove, PA, USA); rabbit anti-DAT antibody and rabbit anti-NET were obtained from Alomone Labs (Jerusalem, Israel); mouse anti-SERT antibody was obtained from Chemicon International (Temecula, CA, USA); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) was obtained from Sigma (St. Louis, MO, USA); rabbit anti-GFAP antibody was obtained from DAKO (Glostrup, Denmark); rhodamine-conjugated goat anti-mouse (for GFAP), anti-rabbit (for GFAP) IgG antibody, and fluorescein-isothiocyanate-conjugated goat anti-rabbit (for DAT and NET) and anti-mouse (for SERT) antibody were obtained from Santa Cruz Biochemistry (Delaware, CA, USA); Dulbecco’s modified Eagle media (DMEM) with or without glucose, penicillin, and streptomycin were obtained from Life Technologies (Grand Island, NY, USA); defined fetal bovine serum was obtained from HyClone Laboratories (Logan, UT, USA); and 2′,7′-dichlorodihydrofluorescein diacetate (H2 DCFDA) and monochlorobimane (MCB) were obtained from Molecular Probe Inc. (Eugene, OR, USA); and alamarBlue was obtained from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Expressing, Double Immunostaining, Staining

    Effects of the simultaneous inhibition of three Na + -dependent monoamine transporters (DAT, NET, and SERT) on dopamine (DA)-induced increases in pentose-phosphate pathway (PPP) flux in cortical (a) and striatal (b) astroglia. Values are the mean ± SD of four flasks. * p

    Journal: ASN NEURO

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

    doi: 10.1177/1759091418775562

    Figure Lengend Snippet: Effects of the simultaneous inhibition of three Na + -dependent monoamine transporters (DAT, NET, and SERT) on dopamine (DA)-induced increases in pentose-phosphate pathway (PPP) flux in cortical (a) and striatal (b) astroglia. Values are the mean ± SD of four flasks. * p

    Article Snippet: Cells were incubated with the primary antibodies (mouse anti-DAT [1:100; Alomone Labs, #AMT-003], anti-NET [1:100; Alomone Labs, #AMT-002], or rabbit ant-SERT [1:200; Chemicon International, MAB1564] antibody, mouse anti-GFAP [1:200; Sigma-Aldrich, #G3893], or rabbit anti-GFAP [1:200; DAKO, #Z0334]) for 2 hr at room temperature.

    Techniques: Inhibition