rabbit anti dat antibody  (Alomone Labs)


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    Name:
    Anti Dopamine Transporter DAT extracellular Antibody
    Description:
    Anti Dopamine Transporter DAT extracellular Antibody AMT 003 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot immunohistochemistry and live cell imaging applications It has been designed to recognize DAT from mouse and rat samples The antibody is unlikely to recognize DAT from human samples
    Catalog Number:
    AMT-003
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    Buy from Supplier


    Structured Review

    Alomone Labs rabbit anti dat antibody
    Anti Dopamine Transporter DAT extracellular Antibody
    Anti Dopamine Transporter DAT extracellular Antibody AMT 003 is a highly specific antibody directed against an epitope of the rat protein The antibody can be used in western blot immunohistochemistry and live cell imaging applications It has been designed to recognize DAT from mouse and rat samples The antibody is unlikely to recognize DAT from human samples
    https://www.bioz.com/result/rabbit anti dat antibody/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti dat antibody - by Bioz Stars, 2021-09
    91/100 stars

    Images

    1) Product Images from "Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway"

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

    Journal: ASN NEURO

    doi: 10.1177/1759091418775562

    Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.
    Figure Legend Snippet: Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Techniques Used: Expressing, Double Immunostaining, Staining

    2) Product Images from "Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway"

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

    Journal: ASN NEURO

    doi: 10.1177/1759091418775562

    Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.
    Figure Legend Snippet: Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Techniques Used: Expressing, Double Immunostaining, Staining

    3) Product Images from "Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway"

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

    Journal: ASN NEURO

    doi: 10.1177/1759091418775562

    Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.
    Figure Legend Snippet: Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Techniques Used: Expressing, Double Immunostaining, Staining

    Effects of the simultaneous inhibition of three Na + -dependent monoamine transporters (DAT, NET, and SERT) on dopamine (DA)-induced increases in pentose-phosphate pathway (PPP) flux in cortical (a) and striatal (b) astroglia. Values are the mean ± SD of four flasks. * p
    Figure Legend Snippet: Effects of the simultaneous inhibition of three Na + -dependent monoamine transporters (DAT, NET, and SERT) on dopamine (DA)-induced increases in pentose-phosphate pathway (PPP) flux in cortical (a) and striatal (b) astroglia. Values are the mean ± SD of four flasks. * p

    Techniques Used: Inhibition

    Related Articles

    Incubation:

    Article Title: Sigma-1 receptor deficiency reduces MPTP-induced parkinsonism and death of dopaminergic neurons
    Article Snippet: .. Coronal paraffin sections (5 μ m) were incubated in rabbit anti-DAT antibody (1 : 200; Alomone Labs, Jerusalem, Israel) at 4 °C overnight. ..

    Article Title: Sigma-1 receptor deficiency reduces MPTP-induced parkinsonism and death of dopaminergic neurons
    Article Snippet: .. The membranes were incubated with the antibody of rabbit anti-NR2B phosphorylation (1 : 1000; Abcam), rabbit anti-DAT (1 : 200; Alomone Labs), rabbit anti-VMAT2 (1 : 500; Millipore, Billerican, MA, USA) and rabbit anti-σ 1 R (1 : 500; Santa Cruz) at 4 °C overnight. ..

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway
    Article Snippet: .. Cells were incubated with the primary antibodies (mouse anti-DAT [1:100; Alomone Labs, #AMT-003], anti-NET [1:100; Alomone Labs, #AMT-002], or rabbit ant-SERT [1:200; Chemicon International, MAB1564] antibody, mouse anti-GFAP [1:200; Sigma-Aldrich, #G3893], or rabbit anti-GFAP [1:200; DAKO, #Z0334]) for 2 hr at room temperature. ..

    Activity Assay:

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway
    Article Snippet: .. Chemicals Chemicals and materials were obtained from the following sources: 2-deoxy-D-[1-14 C]glucose ([14 C]deoxyglucose; specific activity, 2.13 GBq/mmol), Insta-Fluor Plus, and hyamine hydroxide 10-X were obtained from Perkin-Elmer Life Sciences (Boston, MA, USA); D-[1-14 C]glucose (specific activity, 2.035 GBq/mmol) and D-[6-14 C]glucose (specific activity, 2.035 GBq/mmol) were obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA); normal goat serum was obtained from Jackson ImmunoResearch (West Grove, PA, USA); rabbit anti-DAT antibody and rabbit anti-NET were obtained from Alomone Labs (Jerusalem, Israel); mouse anti-SERT antibody was obtained from Chemicon International (Temecula, CA, USA); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) was obtained from Sigma (St. Louis, MO, USA); rabbit anti-GFAP antibody was obtained from DAKO (Glostrup, Denmark); rhodamine-conjugated goat anti-mouse (for GFAP), anti-rabbit (for GFAP) IgG antibody, and fluorescein-isothiocyanate-conjugated goat anti-rabbit (for DAT and NET) and anti-mouse (for SERT) antibody were obtained from Santa Cruz Biochemistry (Delaware, CA, USA); Dulbecco’s modified Eagle media (DMEM) with or without glucose, penicillin, and streptomycin were obtained from Life Technologies (Grand Island, NY, USA); defined fetal bovine serum was obtained from HyClone Laboratories (Logan, UT, USA); and 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) and monochlorobimane (MCB) were obtained from Molecular Probe Inc. (Eugene, OR, USA); and alamarBlue was obtained from Thermo Fisher Scientific (Waltham, MA, USA). ..

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway
    Article Snippet: .. Chemicals and materials were obtained from the following sources: 2-deoxy-D-[1-14 C]glucose ([14 C]deoxyglucose; specific activity, 2.13 GBq/mmol), Insta-Fluor Plus, and hyamine hydroxide 10-X were obtained from Perkin-Elmer Life Sciences (Boston, MA, USA); D-[1-14 C]glucose (specific activity, 2.035 GBq/mmol) and D-[6-14 C]glucose (specific activity, 2.035 GBq/mmol) were obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA); normal goat serum was obtained from Jackson ImmunoResearch (West Grove, PA, USA); rabbit anti-DAT antibody and rabbit anti-NET were obtained from Alomone Labs (Jerusalem, Israel); mouse anti-SERT antibody was obtained from Chemicon International (Temecula, CA, USA); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) was obtained from Sigma (St. Louis, MO, USA); rabbit anti-GFAP antibody was obtained from DAKO (Glostrup, Denmark); rhodamine-conjugated goat anti-mouse (for GFAP), anti-rabbit (for GFAP) IgG antibody, and fluorescein-isothiocyanate-conjugated goat anti-rabbit (for DAT and NET) and anti-mouse (for SERT) antibody were obtained from Santa Cruz Biochemistry (Delaware, CA, USA); Dulbecco’s modified Eagle media (DMEM) with or without glucose, penicillin, and streptomycin were obtained from Life Technologies (Grand Island, NY, USA); defined fetal bovine serum was obtained from HyClone Laboratories (Logan, UT, USA); and 2′,7′-dichlorodihydrofluorescein diacetate (H2 DCFDA) and monochlorobimane (MCB) were obtained from Molecular Probe Inc. (Eugene, OR, USA); and alamarBlue was obtained from Thermo Fisher Scientific (Waltham, MA, USA). ..

    Modification:

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway
    Article Snippet: .. Chemicals Chemicals and materials were obtained from the following sources: 2-deoxy-D-[1-14 C]glucose ([14 C]deoxyglucose; specific activity, 2.13 GBq/mmol), Insta-Fluor Plus, and hyamine hydroxide 10-X were obtained from Perkin-Elmer Life Sciences (Boston, MA, USA); D-[1-14 C]glucose (specific activity, 2.035 GBq/mmol) and D-[6-14 C]glucose (specific activity, 2.035 GBq/mmol) were obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA); normal goat serum was obtained from Jackson ImmunoResearch (West Grove, PA, USA); rabbit anti-DAT antibody and rabbit anti-NET were obtained from Alomone Labs (Jerusalem, Israel); mouse anti-SERT antibody was obtained from Chemicon International (Temecula, CA, USA); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) was obtained from Sigma (St. Louis, MO, USA); rabbit anti-GFAP antibody was obtained from DAKO (Glostrup, Denmark); rhodamine-conjugated goat anti-mouse (for GFAP), anti-rabbit (for GFAP) IgG antibody, and fluorescein-isothiocyanate-conjugated goat anti-rabbit (for DAT and NET) and anti-mouse (for SERT) antibody were obtained from Santa Cruz Biochemistry (Delaware, CA, USA); Dulbecco’s modified Eagle media (DMEM) with or without glucose, penicillin, and streptomycin were obtained from Life Technologies (Grand Island, NY, USA); defined fetal bovine serum was obtained from HyClone Laboratories (Logan, UT, USA); and 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) and monochlorobimane (MCB) were obtained from Molecular Probe Inc. (Eugene, OR, USA); and alamarBlue was obtained from Thermo Fisher Scientific (Waltham, MA, USA). ..

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway
    Article Snippet: .. Chemicals and materials were obtained from the following sources: 2-deoxy-D-[1-14 C]glucose ([14 C]deoxyglucose; specific activity, 2.13 GBq/mmol), Insta-Fluor Plus, and hyamine hydroxide 10-X were obtained from Perkin-Elmer Life Sciences (Boston, MA, USA); D-[1-14 C]glucose (specific activity, 2.035 GBq/mmol) and D-[6-14 C]glucose (specific activity, 2.035 GBq/mmol) were obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA); normal goat serum was obtained from Jackson ImmunoResearch (West Grove, PA, USA); rabbit anti-DAT antibody and rabbit anti-NET were obtained from Alomone Labs (Jerusalem, Israel); mouse anti-SERT antibody was obtained from Chemicon International (Temecula, CA, USA); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) was obtained from Sigma (St. Louis, MO, USA); rabbit anti-GFAP antibody was obtained from DAKO (Glostrup, Denmark); rhodamine-conjugated goat anti-mouse (for GFAP), anti-rabbit (for GFAP) IgG antibody, and fluorescein-isothiocyanate-conjugated goat anti-rabbit (for DAT and NET) and anti-mouse (for SERT) antibody were obtained from Santa Cruz Biochemistry (Delaware, CA, USA); Dulbecco’s modified Eagle media (DMEM) with or without glucose, penicillin, and streptomycin were obtained from Life Technologies (Grand Island, NY, USA); defined fetal bovine serum was obtained from HyClone Laboratories (Logan, UT, USA); and 2′,7′-dichlorodihydrofluorescein diacetate (H2 DCFDA) and monochlorobimane (MCB) were obtained from Molecular Probe Inc. (Eugene, OR, USA); and alamarBlue was obtained from Thermo Fisher Scientific (Waltham, MA, USA). ..

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  • 91
    Alomone Labs rabbit anti dat antibody
    Expression of dopamine transporter <t>(DAT),</t> norepinephrine transporter <t>(NET),</t> and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.
    Rabbit Anti Dat Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti dat antibody/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti dat antibody - by Bioz Stars, 2021-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Journal: ASN NEURO

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

    doi: 10.1177/1759091418775562

    Figure Lengend Snippet: Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Article Snippet: Chemicals Chemicals and materials were obtained from the following sources: 2-deoxy-D-[1-14 C]glucose ([14 C]deoxyglucose; specific activity, 2.13 GBq/mmol), Insta-Fluor Plus, and hyamine hydroxide 10-X were obtained from Perkin-Elmer Life Sciences (Boston, MA, USA); D-[1-14 C]glucose (specific activity, 2.035 GBq/mmol) and D-[6-14 C]glucose (specific activity, 2.035 GBq/mmol) were obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA); normal goat serum was obtained from Jackson ImmunoResearch (West Grove, PA, USA); rabbit anti-DAT antibody and rabbit anti-NET were obtained from Alomone Labs (Jerusalem, Israel); mouse anti-SERT antibody was obtained from Chemicon International (Temecula, CA, USA); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) was obtained from Sigma (St. Louis, MO, USA); rabbit anti-GFAP antibody was obtained from DAKO (Glostrup, Denmark); rhodamine-conjugated goat anti-mouse (for GFAP), anti-rabbit (for GFAP) IgG antibody, and fluorescein-isothiocyanate-conjugated goat anti-rabbit (for DAT and NET) and anti-mouse (for SERT) antibody were obtained from Santa Cruz Biochemistry (Delaware, CA, USA); Dulbecco’s modified Eagle media (DMEM) with or without glucose, penicillin, and streptomycin were obtained from Life Technologies (Grand Island, NY, USA); defined fetal bovine serum was obtained from HyClone Laboratories (Logan, UT, USA); and 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) and monochlorobimane (MCB) were obtained from Molecular Probe Inc. (Eugene, OR, USA); and alamarBlue was obtained from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Expressing, Double Immunostaining, Staining

    Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Journal: ASN NEURO

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

    doi: 10.1177/1759091418775562

    Figure Lengend Snippet: Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Article Snippet: Chemicals and materials were obtained from the following sources: 2-deoxy-D-[1-14 C]glucose ([14 C]deoxyglucose; specific activity, 2.13 GBq/mmol), Insta-Fluor Plus, and hyamine hydroxide 10-X were obtained from Perkin-Elmer Life Sciences (Boston, MA, USA); D-[1-14 C]glucose (specific activity, 2.035 GBq/mmol) and D-[6-14 C]glucose (specific activity, 2.035 GBq/mmol) were obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA); normal goat serum was obtained from Jackson ImmunoResearch (West Grove, PA, USA); rabbit anti-DAT antibody and rabbit anti-NET were obtained from Alomone Labs (Jerusalem, Israel); mouse anti-SERT antibody was obtained from Chemicon International (Temecula, CA, USA); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) was obtained from Sigma (St. Louis, MO, USA); rabbit anti-GFAP antibody was obtained from DAKO (Glostrup, Denmark); rhodamine-conjugated goat anti-mouse (for GFAP), anti-rabbit (for GFAP) IgG antibody, and fluorescein-isothiocyanate-conjugated goat anti-rabbit (for DAT and NET) and anti-mouse (for SERT) antibody were obtained from Santa Cruz Biochemistry (Delaware, CA, USA); Dulbecco’s modified Eagle media (DMEM) with or without glucose, penicillin, and streptomycin were obtained from Life Technologies (Grand Island, NY, USA); defined fetal bovine serum was obtained from HyClone Laboratories (Logan, UT, USA); and 2′,7′-dichlorodihydrofluorescein diacetate (H2 DCFDA) and monochlorobimane (MCB) were obtained from Molecular Probe Inc. (Eugene, OR, USA); and alamarBlue was obtained from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Expressing, Double Immunostaining, Staining

    Effects of the simultaneous inhibition of three Na + -dependent monoamine transporters (DAT, NET, and SERT) on dopamine (DA)-induced increases in pentose-phosphate pathway (PPP) flux in cortical (a) and striatal (b) astroglia. Values are the mean ± SD of four flasks. * p

    Journal: ASN NEURO

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

    doi: 10.1177/1759091418775562

    Figure Lengend Snippet: Effects of the simultaneous inhibition of three Na + -dependent monoamine transporters (DAT, NET, and SERT) on dopamine (DA)-induced increases in pentose-phosphate pathway (PPP) flux in cortical (a) and striatal (b) astroglia. Values are the mean ± SD of four flasks. * p

    Article Snippet: Cells were incubated with the primary antibodies (mouse anti-DAT [1:100; Alomone Labs, #AMT-003], anti-NET [1:100; Alomone Labs, #AMT-002], or rabbit ant-SERT [1:200; Chemicon International, MAB1564] antibody, mouse anti-GFAP [1:200; Sigma-Aldrich, #G3893], or rabbit anti-GFAP [1:200; DAKO, #Z0334]) for 2 hr at room temperature.

    Techniques: Inhibition