rabbit anti net  (Alomone Labs)


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    Name:
    Anti Noradrenaline Transporter NET extracellular Antibody
    Description:
    Anti Noradrenaline Transporter NET extracellular Antibody is directed against an epitope of the mouse noradrenaline norepinephrine transporter Anti Noradrenaline Transporter NET extracellular Antibody AMT 002 can be used in western blot immunohistochemistry and immunocytochemistry applications It has been designed to recognize NET from human mouse and rat samples
    Catalog Number:
    AMT-002
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs rabbit anti net
    Anti Noradrenaline Transporter NET extracellular Antibody
    Anti Noradrenaline Transporter NET extracellular Antibody is directed against an epitope of the mouse noradrenaline norepinephrine transporter Anti Noradrenaline Transporter NET extracellular Antibody AMT 002 can be used in western blot immunohistochemistry and immunocytochemistry applications It has been designed to recognize NET from human mouse and rat samples
    https://www.bioz.com/result/rabbit anti net/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti net - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway"

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

    Journal: ASN NEURO

    doi: 10.1177/1759091418775562

    Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.
    Figure Legend Snippet: Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Techniques Used: Expressing, Double Immunostaining, Staining

    2) Product Images from "18F-meta-fluorobenzylguanidine (18F-mFBG) to monitor changes in norepinephrine transporter expression in response to therapeutic intervention in neuroblastoma models"

    Article Title: 18F-meta-fluorobenzylguanidine (18F-mFBG) to monitor changes in norepinephrine transporter expression in response to therapeutic intervention in neuroblastoma models

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-77788-3

    PET analysis of 18 F-mFBG distribution and NET-1 expression profile. ( a ) Representative coronal slice of Kelly (left) and SK-N-BE(2)C (right) xenograft PET images with corresponding maximum intensity projection CT overlay, 4 h p.i. of 18 F-mFBG. Dotted circles represent tumour region. ( b ) PET quantification of Kelly and SK-N-BE(2)C xenografts 1 and 4 h p.i. of 18 F-mFBG. Data are presented as mean ± SD, n ≥ 5 per group, each dot represents one tumour. *** p
    Figure Legend Snippet: PET analysis of 18 F-mFBG distribution and NET-1 expression profile. ( a ) Representative coronal slice of Kelly (left) and SK-N-BE(2)C (right) xenograft PET images with corresponding maximum intensity projection CT overlay, 4 h p.i. of 18 F-mFBG. Dotted circles represent tumour region. ( b ) PET quantification of Kelly and SK-N-BE(2)C xenografts 1 and 4 h p.i. of 18 F-mFBG. Data are presented as mean ± SD, n ≥ 5 per group, each dot represents one tumour. *** p

    Techniques Used: Positron Emission Tomography, Expressing

    ( a ) Simplified radiosynthesis scheme of 18 F-mFBG by copper-mediated deborylation. ( b ) Total NET-1 protein expression in selected NB cell lines determined by Western blot. Full length blots are presented in Supplementary Figure WB1 . ( c ) 18 F-mFBG (top bar chart) and 123 I-mIBG (bottom bar chart) radiotracer uptake in NB cell lines for 1 h at 37 °C, inhibited with and without 50 µM desipramine (DMI; 15 min pre-incubation). Data are presented as mean ± SEM, n ≥ 2 per group, performed in triplicate. Graphs are generated using GraphPad Prism (v 8.4.1), https://www.graphpad.com .
    Figure Legend Snippet: ( a ) Simplified radiosynthesis scheme of 18 F-mFBG by copper-mediated deborylation. ( b ) Total NET-1 protein expression in selected NB cell lines determined by Western blot. Full length blots are presented in Supplementary Figure WB1 . ( c ) 18 F-mFBG (top bar chart) and 123 I-mIBG (bottom bar chart) radiotracer uptake in NB cell lines for 1 h at 37 °C, inhibited with and without 50 µM desipramine (DMI; 15 min pre-incubation). Data are presented as mean ± SEM, n ≥ 2 per group, performed in triplicate. Graphs are generated using GraphPad Prism (v 8.4.1), https://www.graphpad.com .

    Techniques Used: Expressing, Western Blot, Incubation, Generated

    In vivo assessment of AZD2014 and 18 F-mFBG uptake. ( a ) PET/CT of SK-N-BE(2)C xenografts. PET image and corresponding maximum intensity projection CT overlay, 4 h p.i. of 18 F-mFBG in vehicle (left) and AZD2014 (20 mg/kg/day)(right) treated mice, 3 days. Tumours highlighted with dotted white circle. ( b ) Western blot (n = 4) and ( c ) IHC analysis of SK-N-BE(2)C tumour lysates of animals treated for 3 days with vehicle (V3) or AZD2014 20 mg/kg/day (T3). ( d ) Western blot (n = 2) and ( e ) IHC analysis of Kelly tumour lysates treated for 3 days with vehicle (V3) or AZD2014 25 mg/kg/day (T3). ( f ) Linear regression fit of NET-1 band density vs PET mean quantification in vehicle (r 2 = 0.98) and AZD2014 (r 2 = 0.53) treated tumours (n = 5–6 per group). For Western blots, full-length blots are presented in Supplementary Figures WB3 and WB4 . Graph is generated using GraphPad Prism (v 8.4.1), https://www.graphpad.com .
    Figure Legend Snippet: In vivo assessment of AZD2014 and 18 F-mFBG uptake. ( a ) PET/CT of SK-N-BE(2)C xenografts. PET image and corresponding maximum intensity projection CT overlay, 4 h p.i. of 18 F-mFBG in vehicle (left) and AZD2014 (20 mg/kg/day)(right) treated mice, 3 days. Tumours highlighted with dotted white circle. ( b ) Western blot (n = 4) and ( c ) IHC analysis of SK-N-BE(2)C tumour lysates of animals treated for 3 days with vehicle (V3) or AZD2014 20 mg/kg/day (T3). ( d ) Western blot (n = 2) and ( e ) IHC analysis of Kelly tumour lysates treated for 3 days with vehicle (V3) or AZD2014 25 mg/kg/day (T3). ( f ) Linear regression fit of NET-1 band density vs PET mean quantification in vehicle (r 2 = 0.98) and AZD2014 (r 2 = 0.53) treated tumours (n = 5–6 per group). For Western blots, full-length blots are presented in Supplementary Figures WB3 and WB4 . Graph is generated using GraphPad Prism (v 8.4.1), https://www.graphpad.com .

    Techniques Used: In Vivo, Positron Emission Tomography, Mouse Assay, Western Blot, Immunohistochemistry, Generated

    3) Product Images from "The Role of the Second Extracellular Loop of Norepinephrine Transporter, Neurotrophin-3 and Tropomyosin Receptor Kinase C in T Cells: A Peripheral Biomarker in the Etiology of Schizophrenia"

    Article Title: The Role of the Second Extracellular Loop of Norepinephrine Transporter, Neurotrophin-3 and Tropomyosin Receptor Kinase C in T Cells: A Peripheral Biomarker in the Etiology of Schizophrenia

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22168499

    Predictive results of the CABS-dock server. Legend: computational modelling of protein–peptide docking of NT-3 (1nt3:A) or TrkC (1wwc:A) and the peptide sequence (part of the second extracellular loop of NET: LLNGSVLGNHTKYSK). The docking prediction figures of proteins–peptide correspond to model 1. Clustering details of the ten models are shown in the respective tables.
    Figure Legend Snippet: Predictive results of the CABS-dock server. Legend: computational modelling of protein–peptide docking of NT-3 (1nt3:A) or TrkC (1wwc:A) and the peptide sequence (part of the second extracellular loop of NET: LLNGSVLGNHTKYSK). The docking prediction figures of proteins–peptide correspond to model 1. Clustering details of the ten models are shown in the respective tables.

    Techniques Used: Sequencing

    Levels of NET and TrkC in T cells, and NT-3 in plasma. Legend: quantification of NET and TrkC levels in T cells and NT-3 in plasma, and their respective scatter plots and bar graphs (mean ± SD). ( a ) NEText (45 patients with schizophrenia vs. 31 controls) and TrkC (31 patients with schizophrenia vs. 22 controls) levels were measured by Western blot and normalized with the housekeeping protein β-actin. Relative units refer to the mean of control values after normalization. The results showed a reduction in NET levels ( p
    Figure Legend Snippet: Levels of NET and TrkC in T cells, and NT-3 in plasma. Legend: quantification of NET and TrkC levels in T cells and NT-3 in plasma, and their respective scatter plots and bar graphs (mean ± SD). ( a ) NEText (45 patients with schizophrenia vs. 31 controls) and TrkC (31 patients with schizophrenia vs. 22 controls) levels were measured by Western blot and normalized with the housekeeping protein β-actin. Relative units refer to the mean of control values after normalization. The results showed a reduction in NET levels ( p

    Techniques Used: Western Blot

    4) Product Images from "Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway"

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

    Journal: ASN NEURO

    doi: 10.1177/1759091418775562

    Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.
    Figure Legend Snippet: Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Techniques Used: Expressing, Double Immunostaining, Staining

    5) Product Images from "The Role of the Second Extracellular Loop of Norepinephrine Transporter, Neurotrophin-3 and Tropomyosin Receptor Kinase C in T Cells: A Peripheral Biomarker in the Etiology of Schizophrenia"

    Article Title: The Role of the Second Extracellular Loop of Norepinephrine Transporter, Neurotrophin-3 and Tropomyosin Receptor Kinase C in T Cells: A Peripheral Biomarker in the Etiology of Schizophrenia

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22168499

    Predictive results of the CABS-dock server. Legend: computational modelling of protein–peptide docking of NT-3 (1nt3:A) or TrkC (1wwc:A) and the peptide sequence (part of the second extracellular loop of NET: LLNGSVLGNHTKYSK). The docking prediction figures of proteins–peptide correspond to model 1. Clustering details of the ten models are shown in the respective tables.
    Figure Legend Snippet: Predictive results of the CABS-dock server. Legend: computational modelling of protein–peptide docking of NT-3 (1nt3:A) or TrkC (1wwc:A) and the peptide sequence (part of the second extracellular loop of NET: LLNGSVLGNHTKYSK). The docking prediction figures of proteins–peptide correspond to model 1. Clustering details of the ten models are shown in the respective tables.

    Techniques Used: Sequencing

    Levels of NET and TrkC in T cells, and NT-3 in plasma. Legend: quantification of NET and TrkC levels in T cells and NT-3 in plasma, and their respective scatter plots and bar graphs (mean ± SD). ( a ) NEText (45 patients with schizophrenia vs. 31 controls) and TrkC (31 patients with schizophrenia vs. 22 controls) levels were measured by Western blot and normalized with the housekeeping protein β-actin. Relative units refer to the mean of control values after normalization. The results showed a reduction in NET levels ( p
    Figure Legend Snippet: Levels of NET and TrkC in T cells, and NT-3 in plasma. Legend: quantification of NET and TrkC levels in T cells and NT-3 in plasma, and their respective scatter plots and bar graphs (mean ± SD). ( a ) NEText (45 patients with schizophrenia vs. 31 controls) and TrkC (31 patients with schizophrenia vs. 22 controls) levels were measured by Western blot and normalized with the housekeeping protein β-actin. Relative units refer to the mean of control values after normalization. The results showed a reduction in NET levels ( p

    Techniques Used: Western Blot

    Related Articles

    Incubation:

    Article Title: 18F-meta-fluorobenzylguanidine (18F-mFBG) to monitor changes in norepinephrine transporter expression in response to therapeutic intervention in neuroblastoma models
    Article Snippet: .. The primary NET-1 antibody (AMT-002, Alomone Labs, Jerusalem, Israel), p-S6S240/244 and p-4EBP1T37/46 were added and incubated overnight at 4 °C. ..

    Article Title: The Role of the Second Extracellular Loop of Norepinephrine Transporter, Neurotrophin-3 and Tropomyosin Receptor Kinase C in T Cells: A Peripheral Biomarker in the Etiology of Schizophrenia
    Article Snippet: .. Cell samples (100 µL) were prepared using a lysis buffer (Tris-HCL 50 mM; NaCL 120 mM; IGEPAL 0.5%; NaF 100 mM; NaVO3 2mM) and incubated with the primary antibody overnight at 4 °C in a rotating mixer: anti-NET extracellular rabbit polyclonal antibody 1:500 (AMT-002, Alomone Labs, Jerusalem, Israel), TrkC rabbit polyclonal antibody 1:500 (ab227289, Abcam, Cambridge, UK), or anti-NT3 rabbit polyclonal antibody 1:500 (ANT-003, Alomone Labs, Jerusalem, Israel). ..

    Article Title: Immunoreactivity of Muscarinic Acetylcholine M2 and Serotonin 5-HT2B Receptors, Norepinephrine Transporter and Kir Channels in a Model of Epilepsy
    Article Snippet: .. Primary antibodies against Kir3.1 (mouse monoclonal, 1:200, Alomone Labs, Jerusalem, Israel (APC-005)), Kir6.2 (rabbit polyclonal, 1:200, Alomone Labs, Jerusalem, Israel (APC-020)), muscarinic acetylcholine receptor M2, mAChR (rabbit polyclonal, 1:200, Alomone Labs, Jerusalem, Israel (APC-002)), norepinephrine transporter (rabbit polyclonal, 1:200, Alomone Labs, Jerusalem, Israel (AMT-002)) and serotonin receptor 2B (rabbit polyclonal, 1:200, Alomone Labs, Jerusalem, Israel (ASR-035)) were diluted in PBS and incubated with the slides at 4 °C. ..

    Lysis:

    Article Title: The Role of the Second Extracellular Loop of Norepinephrine Transporter, Neurotrophin-3 and Tropomyosin Receptor Kinase C in T Cells: A Peripheral Biomarker in the Etiology of Schizophrenia
    Article Snippet: .. Cell samples (100 µL) were prepared using a lysis buffer (Tris-HCL 50 mM; NaCL 120 mM; IGEPAL 0.5%; NaF 100 mM; NaVO3 2mM) and incubated with the primary antibody overnight at 4 °C in a rotating mixer: anti-NET extracellular rabbit polyclonal antibody 1:500 (AMT-002, Alomone Labs, Jerusalem, Israel), TrkC rabbit polyclonal antibody 1:500 (ab227289, Abcam, Cambridge, UK), or anti-NT3 rabbit polyclonal antibody 1:500 (ANT-003, Alomone Labs, Jerusalem, Israel). ..

    Activity Assay:

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway
    Article Snippet: .. Chemicals Chemicals and materials were obtained from the following sources: 2-deoxy-D-[1-14 C]glucose ([14 C]deoxyglucose; specific activity, 2.13 GBq/mmol), Insta-Fluor Plus, and hyamine hydroxide 10-X were obtained from Perkin-Elmer Life Sciences (Boston, MA, USA); D-[1-14 C]glucose (specific activity, 2.035 GBq/mmol) and D-[6-14 C]glucose (specific activity, 2.035 GBq/mmol) were obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA); normal goat serum was obtained from Jackson ImmunoResearch (West Grove, PA, USA); rabbit anti-DAT antibody and rabbit anti-NET were obtained from Alomone Labs (Jerusalem, Israel); mouse anti-SERT antibody was obtained from Chemicon International (Temecula, CA, USA); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) was obtained from Sigma (St. Louis, MO, USA); rabbit anti-GFAP antibody was obtained from DAKO (Glostrup, Denmark); rhodamine-conjugated goat anti-mouse (for GFAP), anti-rabbit (for GFAP) IgG antibody, and fluorescein-isothiocyanate-conjugated goat anti-rabbit (for DAT and NET) and anti-mouse (for SERT) antibody were obtained from Santa Cruz Biochemistry (Delaware, CA, USA); Dulbecco’s modified Eagle media (DMEM) with or without glucose, penicillin, and streptomycin were obtained from Life Technologies (Grand Island, NY, USA); defined fetal bovine serum was obtained from HyClone Laboratories (Logan, UT, USA); and 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) and monochlorobimane (MCB) were obtained from Molecular Probe Inc. (Eugene, OR, USA); and alamarBlue was obtained from Thermo Fisher Scientific (Waltham, MA, USA). ..

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway
    Article Snippet: .. Chemicals and materials were obtained from the following sources: 2-deoxy-D-[1-14 C]glucose ([14 C]deoxyglucose; specific activity, 2.13 GBq/mmol), Insta-Fluor Plus, and hyamine hydroxide 10-X were obtained from Perkin-Elmer Life Sciences (Boston, MA, USA); D-[1-14 C]glucose (specific activity, 2.035 GBq/mmol) and D-[6-14 C]glucose (specific activity, 2.035 GBq/mmol) were obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA); normal goat serum was obtained from Jackson ImmunoResearch (West Grove, PA, USA); rabbit anti-DAT antibody and rabbit anti-NET were obtained from Alomone Labs (Jerusalem, Israel); mouse anti-SERT antibody was obtained from Chemicon International (Temecula, CA, USA); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) was obtained from Sigma (St. Louis, MO, USA); rabbit anti-GFAP antibody was obtained from DAKO (Glostrup, Denmark); rhodamine-conjugated goat anti-mouse (for GFAP), anti-rabbit (for GFAP) IgG antibody, and fluorescein-isothiocyanate-conjugated goat anti-rabbit (for DAT and NET) and anti-mouse (for SERT) antibody were obtained from Santa Cruz Biochemistry (Delaware, CA, USA); Dulbecco’s modified Eagle media (DMEM) with or without glucose, penicillin, and streptomycin were obtained from Life Technologies (Grand Island, NY, USA); defined fetal bovine serum was obtained from HyClone Laboratories (Logan, UT, USA); and 2′,7′-dichlorodihydrofluorescein diacetate (H2 DCFDA) and monochlorobimane (MCB) were obtained from Molecular Probe Inc. (Eugene, OR, USA); and alamarBlue was obtained from Thermo Fisher Scientific (Waltham, MA, USA). ..

    Modification:

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway
    Article Snippet: .. Chemicals Chemicals and materials were obtained from the following sources: 2-deoxy-D-[1-14 C]glucose ([14 C]deoxyglucose; specific activity, 2.13 GBq/mmol), Insta-Fluor Plus, and hyamine hydroxide 10-X were obtained from Perkin-Elmer Life Sciences (Boston, MA, USA); D-[1-14 C]glucose (specific activity, 2.035 GBq/mmol) and D-[6-14 C]glucose (specific activity, 2.035 GBq/mmol) were obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA); normal goat serum was obtained from Jackson ImmunoResearch (West Grove, PA, USA); rabbit anti-DAT antibody and rabbit anti-NET were obtained from Alomone Labs (Jerusalem, Israel); mouse anti-SERT antibody was obtained from Chemicon International (Temecula, CA, USA); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) was obtained from Sigma (St. Louis, MO, USA); rabbit anti-GFAP antibody was obtained from DAKO (Glostrup, Denmark); rhodamine-conjugated goat anti-mouse (for GFAP), anti-rabbit (for GFAP) IgG antibody, and fluorescein-isothiocyanate-conjugated goat anti-rabbit (for DAT and NET) and anti-mouse (for SERT) antibody were obtained from Santa Cruz Biochemistry (Delaware, CA, USA); Dulbecco’s modified Eagle media (DMEM) with or without glucose, penicillin, and streptomycin were obtained from Life Technologies (Grand Island, NY, USA); defined fetal bovine serum was obtained from HyClone Laboratories (Logan, UT, USA); and 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) and monochlorobimane (MCB) were obtained from Molecular Probe Inc. (Eugene, OR, USA); and alamarBlue was obtained from Thermo Fisher Scientific (Waltham, MA, USA). ..

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway
    Article Snippet: .. Chemicals and materials were obtained from the following sources: 2-deoxy-D-[1-14 C]glucose ([14 C]deoxyglucose; specific activity, 2.13 GBq/mmol), Insta-Fluor Plus, and hyamine hydroxide 10-X were obtained from Perkin-Elmer Life Sciences (Boston, MA, USA); D-[1-14 C]glucose (specific activity, 2.035 GBq/mmol) and D-[6-14 C]glucose (specific activity, 2.035 GBq/mmol) were obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA); normal goat serum was obtained from Jackson ImmunoResearch (West Grove, PA, USA); rabbit anti-DAT antibody and rabbit anti-NET were obtained from Alomone Labs (Jerusalem, Israel); mouse anti-SERT antibody was obtained from Chemicon International (Temecula, CA, USA); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) was obtained from Sigma (St. Louis, MO, USA); rabbit anti-GFAP antibody was obtained from DAKO (Glostrup, Denmark); rhodamine-conjugated goat anti-mouse (for GFAP), anti-rabbit (for GFAP) IgG antibody, and fluorescein-isothiocyanate-conjugated goat anti-rabbit (for DAT and NET) and anti-mouse (for SERT) antibody were obtained from Santa Cruz Biochemistry (Delaware, CA, USA); Dulbecco’s modified Eagle media (DMEM) with or without glucose, penicillin, and streptomycin were obtained from Life Technologies (Grand Island, NY, USA); defined fetal bovine serum was obtained from HyClone Laboratories (Logan, UT, USA); and 2′,7′-dichlorodihydrofluorescein diacetate (H2 DCFDA) and monochlorobimane (MCB) were obtained from Molecular Probe Inc. (Eugene, OR, USA); and alamarBlue was obtained from Thermo Fisher Scientific (Waltham, MA, USA). ..

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  • 86
    Alomone Labs anti noradrenaline transporter net extracellular antibody
    Predictive results of the CABS-dock server. Legend: computational modelling of protein–peptide docking of NT-3 (1nt3:A) or <t>TrkC</t> (1wwc:A) and the peptide sequence (part of the second extracellular loop of <t>NET:</t> LLNGSVLGNHTKYSK). The docking prediction figures of proteins–peptide correspond to model 1. Clustering details of the ten models are shown in the respective tables.
    Anti Noradrenaline Transporter Net Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti noradrenaline transporter net extracellular antibody/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti noradrenaline transporter net extracellular antibody - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    94
    Alomone Labs rabbit anti net
    Expression of dopamine transporter <t>(DAT),</t> norepinephrine transporter <t>(NET),</t> and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.
    Rabbit Anti Net, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti net/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti net - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Predictive results of the CABS-dock server. Legend: computational modelling of protein–peptide docking of NT-3 (1nt3:A) or TrkC (1wwc:A) and the peptide sequence (part of the second extracellular loop of NET: LLNGSVLGNHTKYSK). The docking prediction figures of proteins–peptide correspond to model 1. Clustering details of the ten models are shown in the respective tables.

    Journal: International Journal of Molecular Sciences

    Article Title: The Role of the Second Extracellular Loop of Norepinephrine Transporter, Neurotrophin-3 and Tropomyosin Receptor Kinase C in T Cells: A Peripheral Biomarker in the Etiology of Schizophrenia

    doi: 10.3390/ijms22168499

    Figure Lengend Snippet: Predictive results of the CABS-dock server. Legend: computational modelling of protein–peptide docking of NT-3 (1nt3:A) or TrkC (1wwc:A) and the peptide sequence (part of the second extracellular loop of NET: LLNGSVLGNHTKYSK). The docking prediction figures of proteins–peptide correspond to model 1. Clustering details of the ten models are shown in the respective tables.

    Article Snippet: We performed five analyses: (1) 1:100 anti-CD3 mouse monoclonal antibody FITC conjugated (MA1-10178, Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1:250 anti-NET extracellular rabbit polyclonal antibody (AMT-002, Alomone Labs, Jerusalem, Israel); (2) 1:100 anti-CD3 mouse monoclonal antibody FITC conjugated (MA1-10178, Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1:500 anti-NT3 rabbit polyclonal antibody (ANT-003, Alomone Labs, Jerusalem, Israel); (3) 1:100 anti-CD3 mouse monoclonal antibody FITC conjugated (MA1-10178, Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1:250 anti-TrkC rabbit polyclonal antibody (sab1306628, Sigma-Aldrich, St. Louis, MO, USA); (4) 1:250 anti-NET extracellular rabbit polyclonal antibody (AMT-002, Alomone Labs, Jerusalem, Israel) and 1:100 anti-NT3 mouse monoclonal antibody (TA500030, Thermo Fisher Scientific, Inc., Waltham, MA, USA); and (5) 1:250 anti-NET extracellular rabbit polyclonal antibody (AMT-002, Alomone Labs, Jerusalem, Israel) and 1:100 anti-TrkC (CP10188, Cell Applications, Inc., San Diego, CA, USA).

    Techniques: Sequencing

    Levels of NET and TrkC in T cells, and NT-3 in plasma. Legend: quantification of NET and TrkC levels in T cells and NT-3 in plasma, and their respective scatter plots and bar graphs (mean ± SD). ( a ) NEText (45 patients with schizophrenia vs. 31 controls) and TrkC (31 patients with schizophrenia vs. 22 controls) levels were measured by Western blot and normalized with the housekeeping protein β-actin. Relative units refer to the mean of control values after normalization. The results showed a reduction in NET levels ( p

    Journal: International Journal of Molecular Sciences

    Article Title: The Role of the Second Extracellular Loop of Norepinephrine Transporter, Neurotrophin-3 and Tropomyosin Receptor Kinase C in T Cells: A Peripheral Biomarker in the Etiology of Schizophrenia

    doi: 10.3390/ijms22168499

    Figure Lengend Snippet: Levels of NET and TrkC in T cells, and NT-3 in plasma. Legend: quantification of NET and TrkC levels in T cells and NT-3 in plasma, and their respective scatter plots and bar graphs (mean ± SD). ( a ) NEText (45 patients with schizophrenia vs. 31 controls) and TrkC (31 patients with schizophrenia vs. 22 controls) levels were measured by Western blot and normalized with the housekeeping protein β-actin. Relative units refer to the mean of control values after normalization. The results showed a reduction in NET levels ( p

    Article Snippet: We performed five analyses: (1) 1:100 anti-CD3 mouse monoclonal antibody FITC conjugated (MA1-10178, Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1:250 anti-NET extracellular rabbit polyclonal antibody (AMT-002, Alomone Labs, Jerusalem, Israel); (2) 1:100 anti-CD3 mouse monoclonal antibody FITC conjugated (MA1-10178, Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1:500 anti-NT3 rabbit polyclonal antibody (ANT-003, Alomone Labs, Jerusalem, Israel); (3) 1:100 anti-CD3 mouse monoclonal antibody FITC conjugated (MA1-10178, Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1:250 anti-TrkC rabbit polyclonal antibody (sab1306628, Sigma-Aldrich, St. Louis, MO, USA); (4) 1:250 anti-NET extracellular rabbit polyclonal antibody (AMT-002, Alomone Labs, Jerusalem, Israel) and 1:100 anti-NT3 mouse monoclonal antibody (TA500030, Thermo Fisher Scientific, Inc., Waltham, MA, USA); and (5) 1:250 anti-NET extracellular rabbit polyclonal antibody (AMT-002, Alomone Labs, Jerusalem, Israel) and 1:100 anti-TrkC (CP10188, Cell Applications, Inc., San Diego, CA, USA).

    Techniques: Western Blot

    Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Journal: ASN NEURO

    Article Title: Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway

    doi: 10.1177/1759091418775562

    Figure Lengend Snippet: Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

    Article Snippet: Chemicals Chemicals and materials were obtained from the following sources: 2-deoxy-D-[1-14 C]glucose ([14 C]deoxyglucose; specific activity, 2.13 GBq/mmol), Insta-Fluor Plus, and hyamine hydroxide 10-X were obtained from Perkin-Elmer Life Sciences (Boston, MA, USA); D-[1-14 C]glucose (specific activity, 2.035 GBq/mmol) and D-[6-14 C]glucose (specific activity, 2.035 GBq/mmol) were obtained from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA); normal goat serum was obtained from Jackson ImmunoResearch (West Grove, PA, USA); rabbit anti-DAT antibody and rabbit anti-NET were obtained from Alomone Labs (Jerusalem, Israel); mouse anti-SERT antibody was obtained from Chemicon International (Temecula, CA, USA); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) was obtained from Sigma (St. Louis, MO, USA); rabbit anti-GFAP antibody was obtained from DAKO (Glostrup, Denmark); rhodamine-conjugated goat anti-mouse (for GFAP), anti-rabbit (for GFAP) IgG antibody, and fluorescein-isothiocyanate-conjugated goat anti-rabbit (for DAT and NET) and anti-mouse (for SERT) antibody were obtained from Santa Cruz Biochemistry (Delaware, CA, USA); Dulbecco’s modified Eagle media (DMEM) with or without glucose, penicillin, and streptomycin were obtained from Life Technologies (Grand Island, NY, USA); defined fetal bovine serum was obtained from HyClone Laboratories (Logan, UT, USA); and 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) and monochlorobimane (MCB) were obtained from Molecular Probe Inc. (Eugene, OR, USA); and alamarBlue was obtained from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Expressing, Double Immunostaining, Staining

    PET analysis of 18 F-mFBG distribution and NET-1 expression profile. ( a ) Representative coronal slice of Kelly (left) and SK-N-BE(2)C (right) xenograft PET images with corresponding maximum intensity projection CT overlay, 4 h p.i. of 18 F-mFBG. Dotted circles represent tumour region. ( b ) PET quantification of Kelly and SK-N-BE(2)C xenografts 1 and 4 h p.i. of 18 F-mFBG. Data are presented as mean ± SD, n ≥ 5 per group, each dot represents one tumour. *** p

    Journal: Scientific Reports

    Article Title: 18F-meta-fluorobenzylguanidine (18F-mFBG) to monitor changes in norepinephrine transporter expression in response to therapeutic intervention in neuroblastoma models

    doi: 10.1038/s41598-020-77788-3

    Figure Lengend Snippet: PET analysis of 18 F-mFBG distribution and NET-1 expression profile. ( a ) Representative coronal slice of Kelly (left) and SK-N-BE(2)C (right) xenograft PET images with corresponding maximum intensity projection CT overlay, 4 h p.i. of 18 F-mFBG. Dotted circles represent tumour region. ( b ) PET quantification of Kelly and SK-N-BE(2)C xenografts 1 and 4 h p.i. of 18 F-mFBG. Data are presented as mean ± SD, n ≥ 5 per group, each dot represents one tumour. *** p

    Article Snippet: The primary NET-1 antibody (AMT-002, Alomone Labs, Jerusalem, Israel), p-S6S240/244 and p-4EBP1T37/46 were added and incubated overnight at 4 °C.

    Techniques: Positron Emission Tomography, Expressing

    ( a ) Simplified radiosynthesis scheme of 18 F-mFBG by copper-mediated deborylation. ( b ) Total NET-1 protein expression in selected NB cell lines determined by Western blot. Full length blots are presented in Supplementary Figure WB1 . ( c ) 18 F-mFBG (top bar chart) and 123 I-mIBG (bottom bar chart) radiotracer uptake in NB cell lines for 1 h at 37 °C, inhibited with and without 50 µM desipramine (DMI; 15 min pre-incubation). Data are presented as mean ± SEM, n ≥ 2 per group, performed in triplicate. Graphs are generated using GraphPad Prism (v 8.4.1), https://www.graphpad.com .

    Journal: Scientific Reports

    Article Title: 18F-meta-fluorobenzylguanidine (18F-mFBG) to monitor changes in norepinephrine transporter expression in response to therapeutic intervention in neuroblastoma models

    doi: 10.1038/s41598-020-77788-3

    Figure Lengend Snippet: ( a ) Simplified radiosynthesis scheme of 18 F-mFBG by copper-mediated deborylation. ( b ) Total NET-1 protein expression in selected NB cell lines determined by Western blot. Full length blots are presented in Supplementary Figure WB1 . ( c ) 18 F-mFBG (top bar chart) and 123 I-mIBG (bottom bar chart) radiotracer uptake in NB cell lines for 1 h at 37 °C, inhibited with and without 50 µM desipramine (DMI; 15 min pre-incubation). Data are presented as mean ± SEM, n ≥ 2 per group, performed in triplicate. Graphs are generated using GraphPad Prism (v 8.4.1), https://www.graphpad.com .

    Article Snippet: The primary NET-1 antibody (AMT-002, Alomone Labs, Jerusalem, Israel), p-S6S240/244 and p-4EBP1T37/46 were added and incubated overnight at 4 °C.

    Techniques: Expressing, Western Blot, Incubation, Generated

    In vivo assessment of AZD2014 and 18 F-mFBG uptake. ( a ) PET/CT of SK-N-BE(2)C xenografts. PET image and corresponding maximum intensity projection CT overlay, 4 h p.i. of 18 F-mFBG in vehicle (left) and AZD2014 (20 mg/kg/day)(right) treated mice, 3 days. Tumours highlighted with dotted white circle. ( b ) Western blot (n = 4) and ( c ) IHC analysis of SK-N-BE(2)C tumour lysates of animals treated for 3 days with vehicle (V3) or AZD2014 20 mg/kg/day (T3). ( d ) Western blot (n = 2) and ( e ) IHC analysis of Kelly tumour lysates treated for 3 days with vehicle (V3) or AZD2014 25 mg/kg/day (T3). ( f ) Linear regression fit of NET-1 band density vs PET mean quantification in vehicle (r 2 = 0.98) and AZD2014 (r 2 = 0.53) treated tumours (n = 5–6 per group). For Western blots, full-length blots are presented in Supplementary Figures WB3 and WB4 . Graph is generated using GraphPad Prism (v 8.4.1), https://www.graphpad.com .

    Journal: Scientific Reports

    Article Title: 18F-meta-fluorobenzylguanidine (18F-mFBG) to monitor changes in norepinephrine transporter expression in response to therapeutic intervention in neuroblastoma models

    doi: 10.1038/s41598-020-77788-3

    Figure Lengend Snippet: In vivo assessment of AZD2014 and 18 F-mFBG uptake. ( a ) PET/CT of SK-N-BE(2)C xenografts. PET image and corresponding maximum intensity projection CT overlay, 4 h p.i. of 18 F-mFBG in vehicle (left) and AZD2014 (20 mg/kg/day)(right) treated mice, 3 days. Tumours highlighted with dotted white circle. ( b ) Western blot (n = 4) and ( c ) IHC analysis of SK-N-BE(2)C tumour lysates of animals treated for 3 days with vehicle (V3) or AZD2014 20 mg/kg/day (T3). ( d ) Western blot (n = 2) and ( e ) IHC analysis of Kelly tumour lysates treated for 3 days with vehicle (V3) or AZD2014 25 mg/kg/day (T3). ( f ) Linear regression fit of NET-1 band density vs PET mean quantification in vehicle (r 2 = 0.98) and AZD2014 (r 2 = 0.53) treated tumours (n = 5–6 per group). For Western blots, full-length blots are presented in Supplementary Figures WB3 and WB4 . Graph is generated using GraphPad Prism (v 8.4.1), https://www.graphpad.com .

    Article Snippet: The primary NET-1 antibody (AMT-002, Alomone Labs, Jerusalem, Israel), p-S6S240/244 and p-4EBP1T37/46 were added and incubated overnight at 4 °C.

    Techniques: In Vivo, Positron Emission Tomography, Mouse Assay, Western Blot, Immunohistochemistry, Generated