anti mc1r antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs anti mc1r antibody
    <t>MC1R</t> Agonists Reduced Proteinuria in Nephrotic Rats
    Anti Mc1r Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mc1r antibody/product/Alomone Labs
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    anti mc1r antibody - by Bioz Stars, 2022-08
    93/100 stars

    Images

    1) Product Images from "Melanocortin 1 Receptor Agonists Reduce Proteinuria"

    Article Title: Melanocortin 1 Receptor Agonists Reduce Proteinuria

    Journal: Journal of the American Society of Nephrology : JASN

    doi: 10.1681/ASN.2009101025

    MC1R Agonists Reduced Proteinuria in Nephrotic Rats
    Figure Legend Snippet: MC1R Agonists Reduced Proteinuria in Nephrotic Rats

    Techniques Used:

    MC1R Is Expressed in the Kidney and in Most Renal Cells
    Figure Legend Snippet: MC1R Is Expressed in the Kidney and in Most Renal Cells

    Techniques Used:

    MC1R is expressed in normal human kidney tissue. MC1R colocalized with synaptopodin in a podocyte-specific manner. (A) Confocal microscopic analysis of cryosections from human kidney tissue confirmed expression of MC1R (green) and synaptopodin (red).
    Figure Legend Snippet: MC1R is expressed in normal human kidney tissue. MC1R colocalized with synaptopodin in a podocyte-specific manner. (A) Confocal microscopic analysis of cryosections from human kidney tissue confirmed expression of MC1R (green) and synaptopodin (red).

    Techniques Used: Expressing

    MC1R is expressed in kidney tissue from a patient with MN. (A) Staining with MC1R (green), synaptopodin (red), and merge (yellow) also confirm MC1R expression in MN patients. (B) Enlarged images of one glomerular capillary loop. Scale bar = 50 μm.
    Figure Legend Snippet: MC1R is expressed in kidney tissue from a patient with MN. (A) Staining with MC1R (green), synaptopodin (red), and merge (yellow) also confirm MC1R expression in MN patients. (B) Enlarged images of one glomerular capillary loop. Scale bar = 50 μm.

    Techniques Used: Staining, Expressing

    MC1R agonist treatment reduces oxidative stress in PHN rats. Urine samples were collected after 4 weeks of treatment. TBARS were measured and related to the creatinine concentration. TBARS were significantly decreased in MS05-treated PHN rats (T, n =
    Figure Legend Snippet: MC1R agonist treatment reduces oxidative stress in PHN rats. Urine samples were collected after 4 weeks of treatment. TBARS were measured and related to the creatinine concentration. TBARS were significantly decreased in MS05-treated PHN rats (T, n =

    Techniques Used: Concentration Assay

    2) Product Images from "Melanocortin 1 Receptor Deficiency in Hematopoietic Cells Promotes the Expansion of Inflammatory Leukocytes in Atherosclerotic Mice"

    Article Title: Melanocortin 1 Receptor Deficiency in Hematopoietic Cells Promotes the Expansion of Inflammatory Leukocytes in Atherosclerotic Mice

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.774013

    Increased leukocyte and lymphocyte counts in the spleen of Apoe -/- mice reconstituted with MC1-R deficient BM. (A–D) Quantification of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), Ly6C high monocytes (CD45 + , CD11b + , CD115 + and Ly6C high ) and neutrophils (CD45 + , CD11b + , Ly6G + ) in the spleen of Apoe -/- recipient mice. (E) Representative dot plots for the gating of NK T cells (CD45 + , TCRβ + , NK1.1 + ), NK cells (CD45 + , TCRβ - , NK1.1 + ), CD4 + T cells (CD45 + , TCRβ + , CD4 + ), CD8 + T cells (CD45 + , TCRβ + , CD8 + ), B cells (CD45 + , TCRβ - , CD19 + , CD11b - ) and CD11b + B cells (CD45 + , TCRβ - , CD19 + , CD11b + ) in the spleen of Apoe -/- BM transplanted mouse. (F) Quantification of splenic lymphocyte subsets in HFD-fed recipient mice. (G) Quantitative real-time PCR (qPCR) analysis of effector CD4 + T cell markers in the spleen of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice. n=8-9 mice per genotype. (H, I) Representative flow cytometry results for the gating and quantification of IFN-γ + and Foxp3 + CD4 + T cells (CD45 + , TCRβ + , CD4 + ) in the spleen of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice. n=4 mice per genotype. (J) Pro-inflammatory cytokine concentrations in the plasma of chow- and HFD-fed Apoe-/ - mice. *P
    Figure Legend Snippet: Increased leukocyte and lymphocyte counts in the spleen of Apoe -/- mice reconstituted with MC1-R deficient BM. (A–D) Quantification of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), Ly6C high monocytes (CD45 + , CD11b + , CD115 + and Ly6C high ) and neutrophils (CD45 + , CD11b + , Ly6G + ) in the spleen of Apoe -/- recipient mice. (E) Representative dot plots for the gating of NK T cells (CD45 + , TCRβ + , NK1.1 + ), NK cells (CD45 + , TCRβ - , NK1.1 + ), CD4 + T cells (CD45 + , TCRβ + , CD4 + ), CD8 + T cells (CD45 + , TCRβ + , CD8 + ), B cells (CD45 + , TCRβ - , CD19 + , CD11b - ) and CD11b + B cells (CD45 + , TCRβ - , CD19 + , CD11b + ) in the spleen of Apoe -/- BM transplanted mouse. (F) Quantification of splenic lymphocyte subsets in HFD-fed recipient mice. (G) Quantitative real-time PCR (qPCR) analysis of effector CD4 + T cell markers in the spleen of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice. n=8-9 mice per genotype. (H, I) Representative flow cytometry results for the gating and quantification of IFN-γ + and Foxp3 + CD4 + T cells (CD45 + , TCRβ + , CD4 + ) in the spleen of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice. n=4 mice per genotype. (J) Pro-inflammatory cytokine concentrations in the plasma of chow- and HFD-fed Apoe-/ - mice. *P

    Techniques Used: Mouse Assay, Real-time Polymerase Chain Reaction, Flow Cytometry

    Hematopoietic MC1-R deficiency increased leukocyte and hematopoietic stem cell counts in the bone marrow of Apoe -/- recipient mice. (A–D) Quantification of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), neutrophils (CD45 + , CD11b + , Ly6G + ) and Ly6C high monocytes (CD45 + , CD11b + , CD115 + and Ly6C high ) in the bone marrow of Apoe -/- and Apoe -/- Mc1r e/e chimeric mice. (E) Representative dot plots for the gating of LSK + (Lin - , Sca-1 + , c-Kit + ), MPP (CD48 + , CD150 - ), HPC (CD48 - , CD150 - ), HSC (CD150 + , CD48 - ) cells in the bone marrow of Apoe-/- recipient mice. (F, G) Quantification of LSK + and HCS (Lin1 - , Sca-1 + , Kit + ; and CD150 + , CD48 - ) cells in the bone marrow. Data are mean ± SEM, *P ≤ 0.05, **P
    Figure Legend Snippet: Hematopoietic MC1-R deficiency increased leukocyte and hematopoietic stem cell counts in the bone marrow of Apoe -/- recipient mice. (A–D) Quantification of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), neutrophils (CD45 + , CD11b + , Ly6G + ) and Ly6C high monocytes (CD45 + , CD11b + , CD115 + and Ly6C high ) in the bone marrow of Apoe -/- and Apoe -/- Mc1r e/e chimeric mice. (E) Representative dot plots for the gating of LSK + (Lin - , Sca-1 + , c-Kit + ), MPP (CD48 + , CD150 - ), HPC (CD48 - , CD150 - ), HSC (CD150 + , CD48 - ) cells in the bone marrow of Apoe-/- recipient mice. (F, G) Quantification of LSK + and HCS (Lin1 - , Sca-1 + , Kit + ; and CD150 + , CD48 - ) cells in the bone marrow. Data are mean ± SEM, *P ≤ 0.05, **P

    Techniques Used: Mouse Assay

    | MC1-R is expressed on splenic CD4 + T cells and its deficiency selectively modulates CCR5 expression. (A) Immunofluorescence staining of MC1-R and CD4 in the mouse spleen. White arrows indicate co-localization of MC1-R and CD4. RP indicates red pulp; WP, white pulp. (B) Western blot analysis of MC1-R protein expression in isolated CD4 + T cell samples from the spleen. The expression of vinculin is shown as loading control. (C) Quantitative real-time PCR (qPCR) analysis of chemokine receptor and adhesion molecule expression in isolated CD4 + T cells from Apoe -/- or Apoe -/- Mc1r e/e mice. Lanes on the right were incubated in anti-Mc1r antibody solution that was premixed with a molar excess of a blocking Mc1r peptide (D, E) Representative Western blots and quantification of CCR5 and β-actin (loading control) in isolated CD4 + T cells lysates from Apoe -/- or Apoe -/- Mc1r e/e mice. (F) Quantification of CCR5 surface expression by flow cytometry in CD4 + and CD8 + T cells from the spleen of Apoe -/- or Apoe -/- Mc1r e/e mice. Data are mean ± SEM, *P
    Figure Legend Snippet: | MC1-R is expressed on splenic CD4 + T cells and its deficiency selectively modulates CCR5 expression. (A) Immunofluorescence staining of MC1-R and CD4 in the mouse spleen. White arrows indicate co-localization of MC1-R and CD4. RP indicates red pulp; WP, white pulp. (B) Western blot analysis of MC1-R protein expression in isolated CD4 + T cell samples from the spleen. The expression of vinculin is shown as loading control. (C) Quantitative real-time PCR (qPCR) analysis of chemokine receptor and adhesion molecule expression in isolated CD4 + T cells from Apoe -/- or Apoe -/- Mc1r e/e mice. Lanes on the right were incubated in anti-Mc1r antibody solution that was premixed with a molar excess of a blocking Mc1r peptide (D, E) Representative Western blots and quantification of CCR5 and β-actin (loading control) in isolated CD4 + T cells lysates from Apoe -/- or Apoe -/- Mc1r e/e mice. (F) Quantification of CCR5 surface expression by flow cytometry in CD4 + and CD8 + T cells from the spleen of Apoe -/- or Apoe -/- Mc1r e/e mice. Data are mean ± SEM, *P

    Techniques Used: Expressing, Immunofluorescence, Staining, Western Blot, Isolation, Real-time Polymerase Chain Reaction, Mouse Assay, Incubation, Blocking Assay, Flow Cytometry

    Hematopoietic MC1-R deficiency does not affect plaque phenotype but significantly alters homing behavior of T cells. (A) Representative images of hematoxylin and eosin (H E) staining of the aortic sinus of Apoe -/- and Apoe -/- Mc1r e/e BM engrafted mice on chow or HFD for 10 weeks. (B) Representative images of Mac-2 (galectin-3), α-SMA (α-smooth muscle actin), Masson trichrome staining and necrotic core areas in the aortic sinus. Necrotic core areas are indicated with dashed lines in H E-stained images. (C) Quantification of plaque area in aortic sinuses. (D–G) Quantification of Mac-2- and α-SMA – positive areas, plaque collagen content and acellular necrotic core areas as percentage of total plaque area. (H) Quantification of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), Ly6C high monocytes (CD45 + , CD11b + , CD115 + , Ly6C high ) and neutrophils (CD45 + , CD11b + , Ly6G + ) by flow cytometry in the aorta of Apoe -/- recipient mice. (I) Experimental design for analyzing homing of different lymphocyte subsets to the para-aortic lymph nodes (paLN) and spleen. Cells were isolated from the spleen of Apoe -/- and Apoe -/- Mc1r e/e mice and injected into recipient Apoe -/- mice. (J–L) Quantification of CD4 + T cells (CD45 + , TCRβ + , CD4 + ), CD8 + T cells (CD45 + , TCRβ + , CD8 + ) and double positive (DP) T cells (CD45 + , TCRβ + , CD4 + , CD8 + ) by flow cytometry in the blood, paLNs and spleen as percentage of injected CD45 + cells. Data are mean ± SEM, **P
    Figure Legend Snippet: Hematopoietic MC1-R deficiency does not affect plaque phenotype but significantly alters homing behavior of T cells. (A) Representative images of hematoxylin and eosin (H E) staining of the aortic sinus of Apoe -/- and Apoe -/- Mc1r e/e BM engrafted mice on chow or HFD for 10 weeks. (B) Representative images of Mac-2 (galectin-3), α-SMA (α-smooth muscle actin), Masson trichrome staining and necrotic core areas in the aortic sinus. Necrotic core areas are indicated with dashed lines in H E-stained images. (C) Quantification of plaque area in aortic sinuses. (D–G) Quantification of Mac-2- and α-SMA – positive areas, plaque collagen content and acellular necrotic core areas as percentage of total plaque area. (H) Quantification of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), Ly6C high monocytes (CD45 + , CD11b + , CD115 + , Ly6C high ) and neutrophils (CD45 + , CD11b + , Ly6G + ) by flow cytometry in the aorta of Apoe -/- recipient mice. (I) Experimental design for analyzing homing of different lymphocyte subsets to the para-aortic lymph nodes (paLN) and spleen. Cells were isolated from the spleen of Apoe -/- and Apoe -/- Mc1r e/e mice and injected into recipient Apoe -/- mice. (J–L) Quantification of CD4 + T cells (CD45 + , TCRβ + , CD4 + ), CD8 + T cells (CD45 + , TCRβ + , CD8 + ) and double positive (DP) T cells (CD45 + , TCRβ + , CD4 + , CD8 + ) by flow cytometry in the blood, paLNs and spleen as percentage of injected CD45 + cells. Data are mean ± SEM, **P

    Techniques Used: Staining, Mouse Assay, Flow Cytometry, Isolation, Injection

    MC1-R deficient CD4 + T cells show impaired CCR5-dependent migration and recycling of the CCR5 receptor. (A–C) Transwell migration assay using splenocytes from Apoe -/- and Apoe -/- Mc1r e/e mice. Cell were allowed to migrate for 3 hours towards the indicated chemokines and migrated CD4 + T cells, CD8 + T cells and Ly6C high monocytes were quantified by flow cytometry as percentage of input CD45 + cells. n=4 mice per genotype (D, E) Quantification of CCR5 surface expression on CD4 + cells and CD8 + T cells that were isolated from Apoe -/- or Apoe -/- Mc1r e/e mice. CCR5 surface expression was quantified during baseline, after CCL5-induced internalization and after withdrawal of CCL5. n=4 mice per genotype. Data are mean ± SEM., *P
    Figure Legend Snippet: MC1-R deficient CD4 + T cells show impaired CCR5-dependent migration and recycling of the CCR5 receptor. (A–C) Transwell migration assay using splenocytes from Apoe -/- and Apoe -/- Mc1r e/e mice. Cell were allowed to migrate for 3 hours towards the indicated chemokines and migrated CD4 + T cells, CD8 + T cells and Ly6C high monocytes were quantified by flow cytometry as percentage of input CD45 + cells. n=4 mice per genotype (D, E) Quantification of CCR5 surface expression on CD4 + cells and CD8 + T cells that were isolated from Apoe -/- or Apoe -/- Mc1r e/e mice. CCR5 surface expression was quantified during baseline, after CCL5-induced internalization and after withdrawal of CCL5. n=4 mice per genotype. Data are mean ± SEM., *P

    Techniques Used: Migration, Transwell Migration Assay, Mouse Assay, Flow Cytometry, Expressing, Isolation

    Hematopoietic MC1-R deficiency induced leukocytosis in Apoe -/- mice. (A) Representative dot plots for the gating of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), neutrophils (CD45 + , CD11b + , CD115 - Ly6G + ) and Ly6C high monocytes (CD45 + , CD11b + , CD115 + , Ly6C high ) in the peripheral blood of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice. (B–E) Quantification of total leukocyte, lymphocyte, Ly6C high monocyte and neutrophil counts in the blood of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice fed a chow or high-fat diet (HFD) for 10 weeks. Data are mean ± SEM, *P
    Figure Legend Snippet: Hematopoietic MC1-R deficiency induced leukocytosis in Apoe -/- mice. (A) Representative dot plots for the gating of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), neutrophils (CD45 + , CD11b + , CD115 - Ly6G + ) and Ly6C high monocytes (CD45 + , CD11b + , CD115 + , Ly6C high ) in the peripheral blood of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice. (B–E) Quantification of total leukocyte, lymphocyte, Ly6C high monocyte and neutrophil counts in the blood of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice fed a chow or high-fat diet (HFD) for 10 weeks. Data are mean ± SEM, *P

    Techniques Used: Mouse Assay

    3) Product Images from "Melanocortin 1 Receptor Agonists Reduce Proteinuria"

    Article Title: Melanocortin 1 Receptor Agonists Reduce Proteinuria

    Journal: Journal of the American Society of Nephrology : JASN

    doi: 10.1681/ASN.2009101025

    MC1R Agonists Reduced Proteinuria in Nephrotic Rats
    Figure Legend Snippet: MC1R Agonists Reduced Proteinuria in Nephrotic Rats

    Techniques Used:

    MC1R Is Expressed in the Kidney and in Most Renal Cells
    Figure Legend Snippet: MC1R Is Expressed in the Kidney and in Most Renal Cells

    Techniques Used:

    MC1R is expressed in normal human kidney tissue. MC1R colocalized with synaptopodin in a podocyte-specific manner. (A) Confocal microscopic analysis of cryosections from human kidney tissue confirmed expression of MC1R (green) and synaptopodin (red).
    Figure Legend Snippet: MC1R is expressed in normal human kidney tissue. MC1R colocalized with synaptopodin in a podocyte-specific manner. (A) Confocal microscopic analysis of cryosections from human kidney tissue confirmed expression of MC1R (green) and synaptopodin (red).

    Techniques Used: Expressing

    MC1R is expressed in kidney tissue from a patient with MN. (A) Staining with MC1R (green), synaptopodin (red), and merge (yellow) also confirm MC1R expression in MN patients. (B) Enlarged images of one glomerular capillary loop. Scale bar = 50 μm.
    Figure Legend Snippet: MC1R is expressed in kidney tissue from a patient with MN. (A) Staining with MC1R (green), synaptopodin (red), and merge (yellow) also confirm MC1R expression in MN patients. (B) Enlarged images of one glomerular capillary loop. Scale bar = 50 μm.

    Techniques Used: Staining, Expressing

    MC1R agonist treatment reduces oxidative stress in PHN rats. Urine samples were collected after 4 weeks of treatment. TBARS were measured and related to the creatinine concentration. TBARS were significantly decreased in MS05-treated PHN rats (T, n =
    Figure Legend Snippet: MC1R agonist treatment reduces oxidative stress in PHN rats. Urine samples were collected after 4 weeks of treatment. TBARS were measured and related to the creatinine concentration. TBARS were significantly decreased in MS05-treated PHN rats (T, n =

    Techniques Used: Concentration Assay

    4) Product Images from "Effects of Melanocortin 1 Receptor Agonists in Experimental Nephropathies"

    Article Title: Effects of Melanocortin 1 Receptor Agonists in Experimental Nephropathies

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0087816

    Blood urea nitrogen (BUN) does not differ in untreated adriamycin mice compared with MC1R agonist treated mice. BUN was measured in plasma samples taken from mice 8–10 days after injection of adriamycin. There was no difference between the groups (n = 9–10, n.s.). Results are presented as mean ± SEM.
    Figure Legend Snippet: Blood urea nitrogen (BUN) does not differ in untreated adriamycin mice compared with MC1R agonist treated mice. BUN was measured in plasma samples taken from mice 8–10 days after injection of adriamycin. There was no difference between the groups (n = 9–10, n.s.). Results are presented as mean ± SEM.

    Techniques Used: Mouse Assay, Injection

    Albuminuria was reduced in MC1R agonist treated PHN rats. After four weeks of MS05 treatment (n = 17), the urinary albumin- to-creatinine ratio (UACR) was significantly reduced compared to untreated PHN (n = 14; p
    Figure Legend Snippet: Albuminuria was reduced in MC1R agonist treated PHN rats. After four weeks of MS05 treatment (n = 17), the urinary albumin- to-creatinine ratio (UACR) was significantly reduced compared to untreated PHN (n = 14; p

    Techniques Used:

    MC1R protein expression. Western blotting was performed in order to detect MC1R protein expression in mouse and human tissue. Left panel: incubation with a rabbit polyclonal MC1R antibody (Alomone Labs). Right panel: incubation with MC1R antibody and control blocking peptide antigen (BP) in order to show antibody specificity. A) mouse whole glomerular lysate, B) cultured wild type mouse podocytes and C) a human malignant melanoma cell line A375. MC1R protein expression was detected at the expected size of 37 kDa (left panel).
    Figure Legend Snippet: MC1R protein expression. Western blotting was performed in order to detect MC1R protein expression in mouse and human tissue. Left panel: incubation with a rabbit polyclonal MC1R antibody (Alomone Labs). Right panel: incubation with MC1R antibody and control blocking peptide antigen (BP) in order to show antibody specificity. A) mouse whole glomerular lysate, B) cultured wild type mouse podocytes and C) a human malignant melanoma cell line A375. MC1R protein expression was detected at the expected size of 37 kDa (left panel).

    Techniques Used: Expressing, Western Blot, Incubation, Blocking Assay, Cell Culture

    MC1R agonists did not reduce albuminuria in adriamycin-treated mice. (A) Five different doses of adriamycin, in the range of 5–10 mg/kg, were intravenously injected and the level of albuminuria was measured after 7 days. The urinary albumin-to-creatinine ratio (UACR) increased with increasing levels of adriamycin. (B) Albuminuria at day 7. Treatment with the MC1R agonist BMS-470539 did not reduce the level of albuminuria for the 8 mg/kg adriamycin dose, on the contrary it slightly increased albuminuria for the 10 mg/kg dose (n = 17–19, p
    Figure Legend Snippet: MC1R agonists did not reduce albuminuria in adriamycin-treated mice. (A) Five different doses of adriamycin, in the range of 5–10 mg/kg, were intravenously injected and the level of albuminuria was measured after 7 days. The urinary albumin-to-creatinine ratio (UACR) increased with increasing levels of adriamycin. (B) Albuminuria at day 7. Treatment with the MC1R agonist BMS-470539 did not reduce the level of albuminuria for the 8 mg/kg adriamycin dose, on the contrary it slightly increased albuminuria for the 10 mg/kg dose (n = 17–19, p

    Techniques Used: Mouse Assay, Injection

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    Alomone Labs anti mc1r antibody
    <t>MC1R</t> Agonists Reduced Proteinuria in Nephrotic Rats
    Anti Mc1r Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mc1r antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mc1r antibody - by Bioz Stars, 2022-08
    93/100 stars
      Buy from Supplier

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    MC1R Agonists Reduced Proteinuria in Nephrotic Rats

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Melanocortin 1 Receptor Agonists Reduce Proteinuria

    doi: 10.1681/ASN.2009101025

    Figure Lengend Snippet: MC1R Agonists Reduced Proteinuria in Nephrotic Rats

    Article Snippet: Membranes were blocked with 5% nonfat dry milk in Tris-buffered saline-Tween at 4°C overnight, followed by incubation with an anti-MC1R antibody (Alomone Labs, Ltd., Israel) at a dilution of 1:500 at 4°C overnight.

    Techniques:

    MC1R Is Expressed in the Kidney and in Most Renal Cells

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Melanocortin 1 Receptor Agonists Reduce Proteinuria

    doi: 10.1681/ASN.2009101025

    Figure Lengend Snippet: MC1R Is Expressed in the Kidney and in Most Renal Cells

    Article Snippet: Membranes were blocked with 5% nonfat dry milk in Tris-buffered saline-Tween at 4°C overnight, followed by incubation with an anti-MC1R antibody (Alomone Labs, Ltd., Israel) at a dilution of 1:500 at 4°C overnight.

    Techniques:

    MC1R is expressed in normal human kidney tissue. MC1R colocalized with synaptopodin in a podocyte-specific manner. (A) Confocal microscopic analysis of cryosections from human kidney tissue confirmed expression of MC1R (green) and synaptopodin (red).

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Melanocortin 1 Receptor Agonists Reduce Proteinuria

    doi: 10.1681/ASN.2009101025

    Figure Lengend Snippet: MC1R is expressed in normal human kidney tissue. MC1R colocalized with synaptopodin in a podocyte-specific manner. (A) Confocal microscopic analysis of cryosections from human kidney tissue confirmed expression of MC1R (green) and synaptopodin (red).

    Article Snippet: Membranes were blocked with 5% nonfat dry milk in Tris-buffered saline-Tween at 4°C overnight, followed by incubation with an anti-MC1R antibody (Alomone Labs, Ltd., Israel) at a dilution of 1:500 at 4°C overnight.

    Techniques: Expressing

    MC1R is expressed in kidney tissue from a patient with MN. (A) Staining with MC1R (green), synaptopodin (red), and merge (yellow) also confirm MC1R expression in MN patients. (B) Enlarged images of one glomerular capillary loop. Scale bar = 50 μm.

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Melanocortin 1 Receptor Agonists Reduce Proteinuria

    doi: 10.1681/ASN.2009101025

    Figure Lengend Snippet: MC1R is expressed in kidney tissue from a patient with MN. (A) Staining with MC1R (green), synaptopodin (red), and merge (yellow) also confirm MC1R expression in MN patients. (B) Enlarged images of one glomerular capillary loop. Scale bar = 50 μm.

    Article Snippet: Membranes were blocked with 5% nonfat dry milk in Tris-buffered saline-Tween at 4°C overnight, followed by incubation with an anti-MC1R antibody (Alomone Labs, Ltd., Israel) at a dilution of 1:500 at 4°C overnight.

    Techniques: Staining, Expressing

    MC1R agonist treatment reduces oxidative stress in PHN rats. Urine samples were collected after 4 weeks of treatment. TBARS were measured and related to the creatinine concentration. TBARS were significantly decreased in MS05-treated PHN rats (T, n =

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Melanocortin 1 Receptor Agonists Reduce Proteinuria

    doi: 10.1681/ASN.2009101025

    Figure Lengend Snippet: MC1R agonist treatment reduces oxidative stress in PHN rats. Urine samples were collected after 4 weeks of treatment. TBARS were measured and related to the creatinine concentration. TBARS were significantly decreased in MS05-treated PHN rats (T, n =

    Article Snippet: Membranes were blocked with 5% nonfat dry milk in Tris-buffered saline-Tween at 4°C overnight, followed by incubation with an anti-MC1R antibody (Alomone Labs, Ltd., Israel) at a dilution of 1:500 at 4°C overnight.

    Techniques: Concentration Assay

    Increased leukocyte and lymphocyte counts in the spleen of Apoe -/- mice reconstituted with MC1-R deficient BM. (A–D) Quantification of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), Ly6C high monocytes (CD45 + , CD11b + , CD115 + and Ly6C high ) and neutrophils (CD45 + , CD11b + , Ly6G + ) in the spleen of Apoe -/- recipient mice. (E) Representative dot plots for the gating of NK T cells (CD45 + , TCRβ + , NK1.1 + ), NK cells (CD45 + , TCRβ - , NK1.1 + ), CD4 + T cells (CD45 + , TCRβ + , CD4 + ), CD8 + T cells (CD45 + , TCRβ + , CD8 + ), B cells (CD45 + , TCRβ - , CD19 + , CD11b - ) and CD11b + B cells (CD45 + , TCRβ - , CD19 + , CD11b + ) in the spleen of Apoe -/- BM transplanted mouse. (F) Quantification of splenic lymphocyte subsets in HFD-fed recipient mice. (G) Quantitative real-time PCR (qPCR) analysis of effector CD4 + T cell markers in the spleen of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice. n=8-9 mice per genotype. (H, I) Representative flow cytometry results for the gating and quantification of IFN-γ + and Foxp3 + CD4 + T cells (CD45 + , TCRβ + , CD4 + ) in the spleen of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice. n=4 mice per genotype. (J) Pro-inflammatory cytokine concentrations in the plasma of chow- and HFD-fed Apoe-/ - mice. *P

    Journal: Frontiers in Immunology

    Article Title: Melanocortin 1 Receptor Deficiency in Hematopoietic Cells Promotes the Expansion of Inflammatory Leukocytes in Atherosclerotic Mice

    doi: 10.3389/fimmu.2021.774013

    Figure Lengend Snippet: Increased leukocyte and lymphocyte counts in the spleen of Apoe -/- mice reconstituted with MC1-R deficient BM. (A–D) Quantification of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), Ly6C high monocytes (CD45 + , CD11b + , CD115 + and Ly6C high ) and neutrophils (CD45 + , CD11b + , Ly6G + ) in the spleen of Apoe -/- recipient mice. (E) Representative dot plots for the gating of NK T cells (CD45 + , TCRβ + , NK1.1 + ), NK cells (CD45 + , TCRβ - , NK1.1 + ), CD4 + T cells (CD45 + , TCRβ + , CD4 + ), CD8 + T cells (CD45 + , TCRβ + , CD8 + ), B cells (CD45 + , TCRβ - , CD19 + , CD11b - ) and CD11b + B cells (CD45 + , TCRβ - , CD19 + , CD11b + ) in the spleen of Apoe -/- BM transplanted mouse. (F) Quantification of splenic lymphocyte subsets in HFD-fed recipient mice. (G) Quantitative real-time PCR (qPCR) analysis of effector CD4 + T cell markers in the spleen of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice. n=8-9 mice per genotype. (H, I) Representative flow cytometry results for the gating and quantification of IFN-γ + and Foxp3 + CD4 + T cells (CD45 + , TCRβ + , CD4 + ) in the spleen of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice. n=4 mice per genotype. (J) Pro-inflammatory cytokine concentrations in the plasma of chow- and HFD-fed Apoe-/ - mice. *P

    Article Snippet: Blots were probed with antibodies against CCR5 (Novus Biologicals, Bio-techne Ltd, UK) and MC1-R (Alomone Labs, Jerusalem, Israel).

    Techniques: Mouse Assay, Real-time Polymerase Chain Reaction, Flow Cytometry

    Hematopoietic MC1-R deficiency increased leukocyte and hematopoietic stem cell counts in the bone marrow of Apoe -/- recipient mice. (A–D) Quantification of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), neutrophils (CD45 + , CD11b + , Ly6G + ) and Ly6C high monocytes (CD45 + , CD11b + , CD115 + and Ly6C high ) in the bone marrow of Apoe -/- and Apoe -/- Mc1r e/e chimeric mice. (E) Representative dot plots for the gating of LSK + (Lin - , Sca-1 + , c-Kit + ), MPP (CD48 + , CD150 - ), HPC (CD48 - , CD150 - ), HSC (CD150 + , CD48 - ) cells in the bone marrow of Apoe-/- recipient mice. (F, G) Quantification of LSK + and HCS (Lin1 - , Sca-1 + , Kit + ; and CD150 + , CD48 - ) cells in the bone marrow. Data are mean ± SEM, *P ≤ 0.05, **P

    Journal: Frontiers in Immunology

    Article Title: Melanocortin 1 Receptor Deficiency in Hematopoietic Cells Promotes the Expansion of Inflammatory Leukocytes in Atherosclerotic Mice

    doi: 10.3389/fimmu.2021.774013

    Figure Lengend Snippet: Hematopoietic MC1-R deficiency increased leukocyte and hematopoietic stem cell counts in the bone marrow of Apoe -/- recipient mice. (A–D) Quantification of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), neutrophils (CD45 + , CD11b + , Ly6G + ) and Ly6C high monocytes (CD45 + , CD11b + , CD115 + and Ly6C high ) in the bone marrow of Apoe -/- and Apoe -/- Mc1r e/e chimeric mice. (E) Representative dot plots for the gating of LSK + (Lin - , Sca-1 + , c-Kit + ), MPP (CD48 + , CD150 - ), HPC (CD48 - , CD150 - ), HSC (CD150 + , CD48 - ) cells in the bone marrow of Apoe-/- recipient mice. (F, G) Quantification of LSK + and HCS (Lin1 - , Sca-1 + , Kit + ; and CD150 + , CD48 - ) cells in the bone marrow. Data are mean ± SEM, *P ≤ 0.05, **P

    Article Snippet: Blots were probed with antibodies against CCR5 (Novus Biologicals, Bio-techne Ltd, UK) and MC1-R (Alomone Labs, Jerusalem, Israel).

    Techniques: Mouse Assay

    | MC1-R is expressed on splenic CD4 + T cells and its deficiency selectively modulates CCR5 expression. (A) Immunofluorescence staining of MC1-R and CD4 in the mouse spleen. White arrows indicate co-localization of MC1-R and CD4. RP indicates red pulp; WP, white pulp. (B) Western blot analysis of MC1-R protein expression in isolated CD4 + T cell samples from the spleen. The expression of vinculin is shown as loading control. (C) Quantitative real-time PCR (qPCR) analysis of chemokine receptor and adhesion molecule expression in isolated CD4 + T cells from Apoe -/- or Apoe -/- Mc1r e/e mice. Lanes on the right were incubated in anti-Mc1r antibody solution that was premixed with a molar excess of a blocking Mc1r peptide (D, E) Representative Western blots and quantification of CCR5 and β-actin (loading control) in isolated CD4 + T cells lysates from Apoe -/- or Apoe -/- Mc1r e/e mice. (F) Quantification of CCR5 surface expression by flow cytometry in CD4 + and CD8 + T cells from the spleen of Apoe -/- or Apoe -/- Mc1r e/e mice. Data are mean ± SEM, *P

    Journal: Frontiers in Immunology

    Article Title: Melanocortin 1 Receptor Deficiency in Hematopoietic Cells Promotes the Expansion of Inflammatory Leukocytes in Atherosclerotic Mice

    doi: 10.3389/fimmu.2021.774013

    Figure Lengend Snippet: | MC1-R is expressed on splenic CD4 + T cells and its deficiency selectively modulates CCR5 expression. (A) Immunofluorescence staining of MC1-R and CD4 in the mouse spleen. White arrows indicate co-localization of MC1-R and CD4. RP indicates red pulp; WP, white pulp. (B) Western blot analysis of MC1-R protein expression in isolated CD4 + T cell samples from the spleen. The expression of vinculin is shown as loading control. (C) Quantitative real-time PCR (qPCR) analysis of chemokine receptor and adhesion molecule expression in isolated CD4 + T cells from Apoe -/- or Apoe -/- Mc1r e/e mice. Lanes on the right were incubated in anti-Mc1r antibody solution that was premixed with a molar excess of a blocking Mc1r peptide (D, E) Representative Western blots and quantification of CCR5 and β-actin (loading control) in isolated CD4 + T cells lysates from Apoe -/- or Apoe -/- Mc1r e/e mice. (F) Quantification of CCR5 surface expression by flow cytometry in CD4 + and CD8 + T cells from the spleen of Apoe -/- or Apoe -/- Mc1r e/e mice. Data are mean ± SEM, *P

    Article Snippet: Blots were probed with antibodies against CCR5 (Novus Biologicals, Bio-techne Ltd, UK) and MC1-R (Alomone Labs, Jerusalem, Israel).

    Techniques: Expressing, Immunofluorescence, Staining, Western Blot, Isolation, Real-time Polymerase Chain Reaction, Mouse Assay, Incubation, Blocking Assay, Flow Cytometry

    Hematopoietic MC1-R deficiency does not affect plaque phenotype but significantly alters homing behavior of T cells. (A) Representative images of hematoxylin and eosin (H E) staining of the aortic sinus of Apoe -/- and Apoe -/- Mc1r e/e BM engrafted mice on chow or HFD for 10 weeks. (B) Representative images of Mac-2 (galectin-3), α-SMA (α-smooth muscle actin), Masson trichrome staining and necrotic core areas in the aortic sinus. Necrotic core areas are indicated with dashed lines in H E-stained images. (C) Quantification of plaque area in aortic sinuses. (D–G) Quantification of Mac-2- and α-SMA – positive areas, plaque collagen content and acellular necrotic core areas as percentage of total plaque area. (H) Quantification of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), Ly6C high monocytes (CD45 + , CD11b + , CD115 + , Ly6C high ) and neutrophils (CD45 + , CD11b + , Ly6G + ) by flow cytometry in the aorta of Apoe -/- recipient mice. (I) Experimental design for analyzing homing of different lymphocyte subsets to the para-aortic lymph nodes (paLN) and spleen. Cells were isolated from the spleen of Apoe -/- and Apoe -/- Mc1r e/e mice and injected into recipient Apoe -/- mice. (J–L) Quantification of CD4 + T cells (CD45 + , TCRβ + , CD4 + ), CD8 + T cells (CD45 + , TCRβ + , CD8 + ) and double positive (DP) T cells (CD45 + , TCRβ + , CD4 + , CD8 + ) by flow cytometry in the blood, paLNs and spleen as percentage of injected CD45 + cells. Data are mean ± SEM, **P

    Journal: Frontiers in Immunology

    Article Title: Melanocortin 1 Receptor Deficiency in Hematopoietic Cells Promotes the Expansion of Inflammatory Leukocytes in Atherosclerotic Mice

    doi: 10.3389/fimmu.2021.774013

    Figure Lengend Snippet: Hematopoietic MC1-R deficiency does not affect plaque phenotype but significantly alters homing behavior of T cells. (A) Representative images of hematoxylin and eosin (H E) staining of the aortic sinus of Apoe -/- and Apoe -/- Mc1r e/e BM engrafted mice on chow or HFD for 10 weeks. (B) Representative images of Mac-2 (galectin-3), α-SMA (α-smooth muscle actin), Masson trichrome staining and necrotic core areas in the aortic sinus. Necrotic core areas are indicated with dashed lines in H E-stained images. (C) Quantification of plaque area in aortic sinuses. (D–G) Quantification of Mac-2- and α-SMA – positive areas, plaque collagen content and acellular necrotic core areas as percentage of total plaque area. (H) Quantification of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), Ly6C high monocytes (CD45 + , CD11b + , CD115 + , Ly6C high ) and neutrophils (CD45 + , CD11b + , Ly6G + ) by flow cytometry in the aorta of Apoe -/- recipient mice. (I) Experimental design for analyzing homing of different lymphocyte subsets to the para-aortic lymph nodes (paLN) and spleen. Cells were isolated from the spleen of Apoe -/- and Apoe -/- Mc1r e/e mice and injected into recipient Apoe -/- mice. (J–L) Quantification of CD4 + T cells (CD45 + , TCRβ + , CD4 + ), CD8 + T cells (CD45 + , TCRβ + , CD8 + ) and double positive (DP) T cells (CD45 + , TCRβ + , CD4 + , CD8 + ) by flow cytometry in the blood, paLNs and spleen as percentage of injected CD45 + cells. Data are mean ± SEM, **P

    Article Snippet: Blots were probed with antibodies against CCR5 (Novus Biologicals, Bio-techne Ltd, UK) and MC1-R (Alomone Labs, Jerusalem, Israel).

    Techniques: Staining, Mouse Assay, Flow Cytometry, Isolation, Injection

    MC1-R deficient CD4 + T cells show impaired CCR5-dependent migration and recycling of the CCR5 receptor. (A–C) Transwell migration assay using splenocytes from Apoe -/- and Apoe -/- Mc1r e/e mice. Cell were allowed to migrate for 3 hours towards the indicated chemokines and migrated CD4 + T cells, CD8 + T cells and Ly6C high monocytes were quantified by flow cytometry as percentage of input CD45 + cells. n=4 mice per genotype (D, E) Quantification of CCR5 surface expression on CD4 + cells and CD8 + T cells that were isolated from Apoe -/- or Apoe -/- Mc1r e/e mice. CCR5 surface expression was quantified during baseline, after CCL5-induced internalization and after withdrawal of CCL5. n=4 mice per genotype. Data are mean ± SEM., *P

    Journal: Frontiers in Immunology

    Article Title: Melanocortin 1 Receptor Deficiency in Hematopoietic Cells Promotes the Expansion of Inflammatory Leukocytes in Atherosclerotic Mice

    doi: 10.3389/fimmu.2021.774013

    Figure Lengend Snippet: MC1-R deficient CD4 + T cells show impaired CCR5-dependent migration and recycling of the CCR5 receptor. (A–C) Transwell migration assay using splenocytes from Apoe -/- and Apoe -/- Mc1r e/e mice. Cell were allowed to migrate for 3 hours towards the indicated chemokines and migrated CD4 + T cells, CD8 + T cells and Ly6C high monocytes were quantified by flow cytometry as percentage of input CD45 + cells. n=4 mice per genotype (D, E) Quantification of CCR5 surface expression on CD4 + cells and CD8 + T cells that were isolated from Apoe -/- or Apoe -/- Mc1r e/e mice. CCR5 surface expression was quantified during baseline, after CCL5-induced internalization and after withdrawal of CCL5. n=4 mice per genotype. Data are mean ± SEM., *P

    Article Snippet: Blots were probed with antibodies against CCR5 (Novus Biologicals, Bio-techne Ltd, UK) and MC1-R (Alomone Labs, Jerusalem, Israel).

    Techniques: Migration, Transwell Migration Assay, Mouse Assay, Flow Cytometry, Expressing, Isolation

    Hematopoietic MC1-R deficiency induced leukocytosis in Apoe -/- mice. (A) Representative dot plots for the gating of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), neutrophils (CD45 + , CD11b + , CD115 - Ly6G + ) and Ly6C high monocytes (CD45 + , CD11b + , CD115 + , Ly6C high ) in the peripheral blood of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice. (B–E) Quantification of total leukocyte, lymphocyte, Ly6C high monocyte and neutrophil counts in the blood of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice fed a chow or high-fat diet (HFD) for 10 weeks. Data are mean ± SEM, *P

    Journal: Frontiers in Immunology

    Article Title: Melanocortin 1 Receptor Deficiency in Hematopoietic Cells Promotes the Expansion of Inflammatory Leukocytes in Atherosclerotic Mice

    doi: 10.3389/fimmu.2021.774013

    Figure Lengend Snippet: Hematopoietic MC1-R deficiency induced leukocytosis in Apoe -/- mice. (A) Representative dot plots for the gating of total leukocytes (CD45 + ), lymphocytes (CD45 + , CD11b - ), neutrophils (CD45 + , CD11b + , CD115 - Ly6G + ) and Ly6C high monocytes (CD45 + , CD11b + , CD115 + , Ly6C high ) in the peripheral blood of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice. (B–E) Quantification of total leukocyte, lymphocyte, Ly6C high monocyte and neutrophil counts in the blood of Apoe -/- and Apoe -/- Mc1r e/e BM transplanted mice fed a chow or high-fat diet (HFD) for 10 weeks. Data are mean ± SEM, *P

    Article Snippet: Blots were probed with antibodies against CCR5 (Novus Biologicals, Bio-techne Ltd, UK) and MC1-R (Alomone Labs, Jerusalem, Israel).

    Techniques: Mouse Assay

    Blood urea nitrogen (BUN) does not differ in untreated adriamycin mice compared with MC1R agonist treated mice. BUN was measured in plasma samples taken from mice 8–10 days after injection of adriamycin. There was no difference between the groups (n = 9–10, n.s.). Results are presented as mean ± SEM.

    Journal: PLoS ONE

    Article Title: Effects of Melanocortin 1 Receptor Agonists in Experimental Nephropathies

    doi: 10.1371/journal.pone.0087816

    Figure Lengend Snippet: Blood urea nitrogen (BUN) does not differ in untreated adriamycin mice compared with MC1R agonist treated mice. BUN was measured in plasma samples taken from mice 8–10 days after injection of adriamycin. There was no difference between the groups (n = 9–10, n.s.). Results are presented as mean ± SEM.

    Article Snippet: The following antibodies and dilutions were used for detection of specific proteins: anti-MC1R antibody 1∶500 (AMR-020, Alomone Labs, Ltd., Israel) and anti-actin goat polyclonal antibody 1∶1000 (sc-1615, Santa Cruz Biotechnology, Inc., Dallas, TX).

    Techniques: Mouse Assay, Injection

    Albuminuria was reduced in MC1R agonist treated PHN rats. After four weeks of MS05 treatment (n = 17), the urinary albumin- to-creatinine ratio (UACR) was significantly reduced compared to untreated PHN (n = 14; p

    Journal: PLoS ONE

    Article Title: Effects of Melanocortin 1 Receptor Agonists in Experimental Nephropathies

    doi: 10.1371/journal.pone.0087816

    Figure Lengend Snippet: Albuminuria was reduced in MC1R agonist treated PHN rats. After four weeks of MS05 treatment (n = 17), the urinary albumin- to-creatinine ratio (UACR) was significantly reduced compared to untreated PHN (n = 14; p

    Article Snippet: The following antibodies and dilutions were used for detection of specific proteins: anti-MC1R antibody 1∶500 (AMR-020, Alomone Labs, Ltd., Israel) and anti-actin goat polyclonal antibody 1∶1000 (sc-1615, Santa Cruz Biotechnology, Inc., Dallas, TX).

    Techniques:

    MC1R protein expression. Western blotting was performed in order to detect MC1R protein expression in mouse and human tissue. Left panel: incubation with a rabbit polyclonal MC1R antibody (Alomone Labs). Right panel: incubation with MC1R antibody and control blocking peptide antigen (BP) in order to show antibody specificity. A) mouse whole glomerular lysate, B) cultured wild type mouse podocytes and C) a human malignant melanoma cell line A375. MC1R protein expression was detected at the expected size of 37 kDa (left panel).

    Journal: PLoS ONE

    Article Title: Effects of Melanocortin 1 Receptor Agonists in Experimental Nephropathies

    doi: 10.1371/journal.pone.0087816

    Figure Lengend Snippet: MC1R protein expression. Western blotting was performed in order to detect MC1R protein expression in mouse and human tissue. Left panel: incubation with a rabbit polyclonal MC1R antibody (Alomone Labs). Right panel: incubation with MC1R antibody and control blocking peptide antigen (BP) in order to show antibody specificity. A) mouse whole glomerular lysate, B) cultured wild type mouse podocytes and C) a human malignant melanoma cell line A375. MC1R protein expression was detected at the expected size of 37 kDa (left panel).

    Article Snippet: The following antibodies and dilutions were used for detection of specific proteins: anti-MC1R antibody 1∶500 (AMR-020, Alomone Labs, Ltd., Israel) and anti-actin goat polyclonal antibody 1∶1000 (sc-1615, Santa Cruz Biotechnology, Inc., Dallas, TX).

    Techniques: Expressing, Western Blot, Incubation, Blocking Assay, Cell Culture

    MC1R agonists did not reduce albuminuria in adriamycin-treated mice. (A) Five different doses of adriamycin, in the range of 5–10 mg/kg, were intravenously injected and the level of albuminuria was measured after 7 days. The urinary albumin-to-creatinine ratio (UACR) increased with increasing levels of adriamycin. (B) Albuminuria at day 7. Treatment with the MC1R agonist BMS-470539 did not reduce the level of albuminuria for the 8 mg/kg adriamycin dose, on the contrary it slightly increased albuminuria for the 10 mg/kg dose (n = 17–19, p

    Journal: PLoS ONE

    Article Title: Effects of Melanocortin 1 Receptor Agonists in Experimental Nephropathies

    doi: 10.1371/journal.pone.0087816

    Figure Lengend Snippet: MC1R agonists did not reduce albuminuria in adriamycin-treated mice. (A) Five different doses of adriamycin, in the range of 5–10 mg/kg, were intravenously injected and the level of albuminuria was measured after 7 days. The urinary albumin-to-creatinine ratio (UACR) increased with increasing levels of adriamycin. (B) Albuminuria at day 7. Treatment with the MC1R agonist BMS-470539 did not reduce the level of albuminuria for the 8 mg/kg adriamycin dose, on the contrary it slightly increased albuminuria for the 10 mg/kg dose (n = 17–19, p

    Article Snippet: The following antibodies and dilutions were used for detection of specific proteins: anti-MC1R antibody 1∶500 (AMR-020, Alomone Labs, Ltd., Israel) and anti-actin goat polyclonal antibody 1∶1000 (sc-1615, Santa Cruz Biotechnology, Inc., Dallas, TX).

    Techniques: Mouse Assay, Injection