chrm3  (Alomone Labs)


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    Alomone Labs chrm3
    Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( <t>Chrm3</t> ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p
    Chrm3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chrm3/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chrm3 - by Bioz Stars, 2022-07
    94/100 stars

    Images

    1) Product Images from "Induction of detrusor underactivity by extensive vascular endothelial damages of iliac arteries in a rat model and its pathophysiology in the genetic levels"

    Article Title: Induction of detrusor underactivity by extensive vascular endothelial damages of iliac arteries in a rat model and its pathophysiology in the genetic levels

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-52811-4

    Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( Chrm3 ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p
    Figure Legend Snippet: Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( Chrm3 ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p

    Techniques Used: Polymerase Chain Reaction, Western Blot, Expressing, Immunohistochemistry, Staining

    2) Product Images from "Inducible Deletion of YAP and TAZ in Adult Mouse Smooth Muscle Causes Rapid and Lethal Colonic Pseudo-Obstruction"

    Article Title: Inducible Deletion of YAP and TAZ in Adult Mouse Smooth Muscle Causes Rapid and Lethal Colonic Pseudo-Obstruction

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    doi: 10.1016/j.jcmgh.2020.09.014

    Correlation between YAP/TAZ, MRTF/SRF, and muscarinic receptors in the human GI tract and urinary bladder . ( A ) For the table, RNA sequencing data from GTExPortal.org was used to correlate mRNA levels of YAP1 , TEAD3 , and WWTR1 vs MYOCD , MRTFA , SRF , CHRM2 , and CHRM3 . Spearman Rho values for the indicated correlations are given for 5 sections of the GI tract and the urinary bladder. All Spearman Rho values in bold have a P value less than .0001. Examples of correlations in the transverse colon are highlighted in gray. ( B ) Examples of correlations in the transverse colon are plotted. White lines are best-fit linear regression lines. eso GEJ, esophagus gastroesophageal junction; colon s., colon sigmoid; colon t., colon transverse; ter. ileum, terminal ileum; TPM, transcripts per million.
    Figure Legend Snippet: Correlation between YAP/TAZ, MRTF/SRF, and muscarinic receptors in the human GI tract and urinary bladder . ( A ) For the table, RNA sequencing data from GTExPortal.org was used to correlate mRNA levels of YAP1 , TEAD3 , and WWTR1 vs MYOCD , MRTFA , SRF , CHRM2 , and CHRM3 . Spearman Rho values for the indicated correlations are given for 5 sections of the GI tract and the urinary bladder. All Spearman Rho values in bold have a P value less than .0001. Examples of correlations in the transverse colon are highlighted in gray. ( B ) Examples of correlations in the transverse colon are plotted. White lines are best-fit linear regression lines. eso GEJ, esophagus gastroesophageal junction; colon s., colon sigmoid; colon t., colon transverse; ter. ileum, terminal ileum; TPM, transcripts per million.

    Techniques Used: RNA Sequencing Assay

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    Alomone Labs chrm3
    Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( <t>Chrm3</t> ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p
    Chrm3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chrm3/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chrm3 - by Bioz Stars, 2022-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( Chrm3 ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p

    Journal: Scientific Reports

    Article Title: Induction of detrusor underactivity by extensive vascular endothelial damages of iliac arteries in a rat model and its pathophysiology in the genetic levels

    doi: 10.1038/s41598-019-52811-4

    Figure Lengend Snippet: Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( Chrm3 ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p

    Article Snippet: Protein levels were assessed from 50 μg of extracts separated using 10% SDS-PAGE gels by probing with antibodies specific to P2rx1 (APR-001, Alomone Labs), Chrm2 (AMR-002, Alomone Labs,), Chrm3 (AMR-006, Alomone Labs, Israel), α-SMA (ab5694, Rabbit, Abcam), vimentin (sc-6260, Santa Cruz Biotechnology Inc., CA), and β-actin (Sigma-Aldrich).

    Techniques: Polymerase Chain Reaction, Western Blot, Expressing, Immunohistochemistry, Staining

    Correlation between YAP/TAZ, MRTF/SRF, and muscarinic receptors in the human GI tract and urinary bladder . ( A ) For the table, RNA sequencing data from GTExPortal.org was used to correlate mRNA levels of YAP1 , TEAD3 , and WWTR1 vs MYOCD , MRTFA , SRF , CHRM2 , and CHRM3 . Spearman Rho values for the indicated correlations are given for 5 sections of the GI tract and the urinary bladder. All Spearman Rho values in bold have a P value less than .0001. Examples of correlations in the transverse colon are highlighted in gray. ( B ) Examples of correlations in the transverse colon are plotted. White lines are best-fit linear regression lines. eso GEJ, esophagus gastroesophageal junction; colon s., colon sigmoid; colon t., colon transverse; ter. ileum, terminal ileum; TPM, transcripts per million.

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Inducible Deletion of YAP and TAZ in Adult Mouse Smooth Muscle Causes Rapid and Lethal Colonic Pseudo-Obstruction

    doi: 10.1016/j.jcmgh.2020.09.014

    Figure Lengend Snippet: Correlation between YAP/TAZ, MRTF/SRF, and muscarinic receptors in the human GI tract and urinary bladder . ( A ) For the table, RNA sequencing data from GTExPortal.org was used to correlate mRNA levels of YAP1 , TEAD3 , and WWTR1 vs MYOCD , MRTFA , SRF , CHRM2 , and CHRM3 . Spearman Rho values for the indicated correlations are given for 5 sections of the GI tract and the urinary bladder. All Spearman Rho values in bold have a P value less than .0001. Examples of correlations in the transverse colon are highlighted in gray. ( B ) Examples of correlations in the transverse colon are plotted. White lines are best-fit linear regression lines. eso GEJ, esophagus gastroesophageal junction; colon s., colon sigmoid; colon t., colon transverse; ter. ileum, terminal ileum; TPM, transcripts per million.

    Article Snippet: Specific proteins were detected using commercially available primary antibodies: YAP/TAZ (cat. 8418, 1:1000; Cell Signaling Technology), α-actin (cat. A5228, 1:1000; Sigma-Aldrich), smooth muscle myosin heavy chain (cat. ab53219, 1:1000; abcam), Chrm2 (cat. AMR-002, 1:1000; alomone labs, Jerusalem), Chrm3 (cat. AMR-006, 1:200; alomone labs), SRF (cat. 5147S, 1:1000; Cell Signaling Technology), and myosin light chain (3672S, 1:1000; Cell Signaling Technology).

    Techniques: RNA Sequencing Assay

    Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P

    Journal: International Journal of Molecular Medicine

    Article Title: Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling

    doi: 10.3892/ijmm.2018.3960

    Figure Lengend Snippet: Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P

    Article Snippet: The pretreated sections were then incubated with anti-mAChR M2 (1:200, cat. no. AMR-002; Alomone Labs) or anti-mAChR M3 (1:200, cat. no. AMR-006; Alomone Labs) antibodies diluted 1:1,000 in blocking buffer.

    Techniques: Expressing, Western Blot, Immunofluorescence, Standard Deviation

    Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group).  * P

    Journal: International Journal of Molecular Medicine

    Article Title: Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling

    doi: 10.3892/ijmm.2018.3960

    Figure Lengend Snippet: Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P

    Article Snippet: The pretreated sections were then incubated with anti-mAChR M2 (1:200, cat. no. AMR-002; Alomone Labs) or anti-mAChR M3 (1:200, cat. no. AMR-006; Alomone Labs) antibodies diluted 1:1,000 in blocking buffer.

    Techniques: Expressing, Western Blot, Immunofluorescence, Standard Deviation