chrm2  (Alomone Labs)


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    Structured Review

    Alomone Labs chrm2
    Correlation between YAP/TAZ, MRTF/SRF, and muscarinic receptors in the human GI tract and urinary bladder . ( A ) For the table, RNA sequencing data from GTExPortal.org was used to correlate mRNA levels of YAP1 , TEAD3 , and WWTR1 vs MYOCD , MRTFA , SRF , <t>CHRM2</t> , and CHRM3 . Spearman Rho values for the indicated correlations are given for 5 sections of the GI tract and the urinary bladder. All Spearman Rho values in bold have a P value less than .0001. Examples of correlations in the transverse colon are highlighted in gray. ( B ) Examples of correlations in the transverse colon are plotted. White lines are best-fit linear regression lines. eso GEJ, esophagus gastroesophageal junction; colon s., colon sigmoid; colon t., colon transverse; ter. ileum, terminal ileum; TPM, transcripts per million.
    Chrm2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chrm2/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chrm2 - by Bioz Stars, 2022-07
    93/100 stars

    Images

    1) Product Images from "Inducible Deletion of YAP and TAZ in Adult Mouse Smooth Muscle Causes Rapid and Lethal Colonic Pseudo-Obstruction"

    Article Title: Inducible Deletion of YAP and TAZ in Adult Mouse Smooth Muscle Causes Rapid and Lethal Colonic Pseudo-Obstruction

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    doi: 10.1016/j.jcmgh.2020.09.014

    Correlation between YAP/TAZ, MRTF/SRF, and muscarinic receptors in the human GI tract and urinary bladder . ( A ) For the table, RNA sequencing data from GTExPortal.org was used to correlate mRNA levels of YAP1 , TEAD3 , and WWTR1 vs MYOCD , MRTFA , SRF , CHRM2 , and CHRM3 . Spearman Rho values for the indicated correlations are given for 5 sections of the GI tract and the urinary bladder. All Spearman Rho values in bold have a P value less than .0001. Examples of correlations in the transverse colon are highlighted in gray. ( B ) Examples of correlations in the transverse colon are plotted. White lines are best-fit linear regression lines. eso GEJ, esophagus gastroesophageal junction; colon s., colon sigmoid; colon t., colon transverse; ter. ileum, terminal ileum; TPM, transcripts per million.
    Figure Legend Snippet: Correlation between YAP/TAZ, MRTF/SRF, and muscarinic receptors in the human GI tract and urinary bladder . ( A ) For the table, RNA sequencing data from GTExPortal.org was used to correlate mRNA levels of YAP1 , TEAD3 , and WWTR1 vs MYOCD , MRTFA , SRF , CHRM2 , and CHRM3 . Spearman Rho values for the indicated correlations are given for 5 sections of the GI tract and the urinary bladder. All Spearman Rho values in bold have a P value less than .0001. Examples of correlations in the transverse colon are highlighted in gray. ( B ) Examples of correlations in the transverse colon are plotted. White lines are best-fit linear regression lines. eso GEJ, esophagus gastroesophageal junction; colon s., colon sigmoid; colon t., colon transverse; ter. ileum, terminal ileum; TPM, transcripts per million.

    Techniques Used: RNA Sequencing Assay

    2) Product Images from "Induction of detrusor underactivity by extensive vascular endothelial damages of iliac arteries in a rat model and its pathophysiology in the genetic levels"

    Article Title: Induction of detrusor underactivity by extensive vascular endothelial damages of iliac arteries in a rat model and its pathophysiology in the genetic levels

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-52811-4

    Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( Chrm3 ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p
    Figure Legend Snippet: Repression of P2rx1 by progressive vascular endothelial damage. ( a – c ) RQ-PCR ( a ) and western blot ( b , c ) analysis of the expressions of the cholinergic receptor muscarinic 2 ( Chrm2 ), cholinergic receptor muscarinic 3 ( Chrm3 ), and purinergic receptor P2X 1 ( P2rx1 ) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh . For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. ( d , e ) Representative images ( d ) and quantification analysis ( e ) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p

    Techniques Used: Polymerase Chain Reaction, Western Blot, Expressing, Immunohistochemistry, Staining

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    Alomone Labs anti machr m2 antibody
    Expression of mAChRs transcript in the transverse colon. The levels of <t>mAChR</t> M2 and M3 transcripts in the total mRNA of transverse colons were measured by RT-PCR using specific primers. After the intensity of each band was determined using an imaging densitometer, the relative levels of mAChR M2 and M3 transcripts were calculated based on the intensity of actin transcripts. Five to six rats per group were assayed in triplicate by RT-PCR assays. Data represent the means±SD of three replicates. a, p
    Anti Machr M2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti machr m2 antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti machr m2 antibody - by Bioz Stars, 2022-07
    93/100 stars
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    Expression of mAChRs transcript in the transverse colon. The levels of mAChR M2 and M3 transcripts in the total mRNA of transverse colons were measured by RT-PCR using specific primers. After the intensity of each band was determined using an imaging densitometer, the relative levels of mAChR M2 and M3 transcripts were calculated based on the intensity of actin transcripts. Five to six rats per group were assayed in triplicate by RT-PCR assays. Data represent the means±SD of three replicates. a, p

    Journal: PLoS ONE

    Article Title: Gallotannin-Enriched Extract Isolated from Galla Rhois May Be a Functional Candidate with Laxative Effects for Treatment of Loperamide-Induced Constipation of SD Rats

    doi: 10.1371/journal.pone.0161144

    Figure Lengend Snippet: Expression of mAChRs transcript in the transverse colon. The levels of mAChR M2 and M3 transcripts in the total mRNA of transverse colons were measured by RT-PCR using specific primers. After the intensity of each band was determined using an imaging densitometer, the relative levels of mAChR M2 and M3 transcripts were calculated based on the intensity of actin transcripts. Five to six rats per group were assayed in triplicate by RT-PCR assays. Data represent the means±SD of three replicates. a, p

    Article Snippet: Western blotting Total proteins collected from the transverse colons of subset groups (No, GEGR, Lop+vehicle, Lop+LoGEGR, Lop+MeGEGR, Lop+HiGEGR and Lop+BS treated SD rats) were separated by 4%–20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for 3 h, after which the resolved proteins were transferred to nitrocellulose membranes for 2 h at 40 V. Each membrane was then incubated separately with primary antibody, anti-mAChR M2 antibody (Alomone Labs, Jerusalem, Israel), anti-PI-3K (Cell Signaling Technology Inc., Cambridge, MA, USA), anti-p-PI3K (Cell Signaling Technology Inc., Cambridge, MA, USA), anti-mAChR M3 antibody (Alomone Labs, Jerusalem, Israel), anti-PKC (Cell Signaling Technology Inc.), anti-p-PKC (Cell Signaling Technology Inc.), anti-Gα (Abcame, Cambridge, UK) or anti-actin (Sigma-Aldrich Co.) overnight at 4°C.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Imaging

    Expression of key proteins in the mAChR M2 and M3 signaling pathway. The expression of several related proteins in the mAChR M2 and M3 signaling pathway was measured by Western blot analysis using HRP-labeled anti-rabbit IgG antibody. After the intensity of each band was determined using an imaging densitometer, the relative levels of six proteins were calculated based on the intensity of actin protein. Five to six rats per group were assayed in triplicate by Western blotting. Data represent the means ± SD of three replicates. a, p

    Journal: PLoS ONE

    Article Title: Gallotannin-Enriched Extract Isolated from Galla Rhois May Be a Functional Candidate with Laxative Effects for Treatment of Loperamide-Induced Constipation of SD Rats

    doi: 10.1371/journal.pone.0161144

    Figure Lengend Snippet: Expression of key proteins in the mAChR M2 and M3 signaling pathway. The expression of several related proteins in the mAChR M2 and M3 signaling pathway was measured by Western blot analysis using HRP-labeled anti-rabbit IgG antibody. After the intensity of each band was determined using an imaging densitometer, the relative levels of six proteins were calculated based on the intensity of actin protein. Five to six rats per group were assayed in triplicate by Western blotting. Data represent the means ± SD of three replicates. a, p

    Article Snippet: Western blotting Total proteins collected from the transverse colons of subset groups (No, GEGR, Lop+vehicle, Lop+LoGEGR, Lop+MeGEGR, Lop+HiGEGR and Lop+BS treated SD rats) were separated by 4%–20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for 3 h, after which the resolved proteins were transferred to nitrocellulose membranes for 2 h at 40 V. Each membrane was then incubated separately with primary antibody, anti-mAChR M2 antibody (Alomone Labs, Jerusalem, Israel), anti-PI-3K (Cell Signaling Technology Inc., Cambridge, MA, USA), anti-p-PI3K (Cell Signaling Technology Inc., Cambridge, MA, USA), anti-mAChR M3 antibody (Alomone Labs, Jerusalem, Israel), anti-PKC (Cell Signaling Technology Inc.), anti-p-PKC (Cell Signaling Technology Inc.), anti-Gα (Abcame, Cambridge, UK) or anti-actin (Sigma-Aldrich Co.) overnight at 4°C.

    Techniques: Expressing, Western Blot, Labeling, Imaging

    Immunostaining of HL-1 cells. Immunofluorescent staining of HL-1 cells and the M2 GIRK1/4 cell line stained for GIRK4 and the M2 receptor, respectively.  Arrows  show potential membrane localisation. In the  lower panels  of the figure, two controls are shown. In parental HEK293 cells without GIRK4 expression, the GIRK4 antibody does not stain the cells. Incubation of the M2 GIRK1/4 cell line with only the secondary antibody and not the primary does not lead to staining

    Journal: Pflugers Archiv

    Article Title: HL-1 cells express an inwardly rectifying K+ current activated via muscarinic receptors comparable to that in mouse atrial myocytes

    doi: 10.1007/s00424-010-0799-z

    Figure Lengend Snippet: Immunostaining of HL-1 cells. Immunofluorescent staining of HL-1 cells and the M2 GIRK1/4 cell line stained for GIRK4 and the M2 receptor, respectively. Arrows show potential membrane localisation. In the lower panels of the figure, two controls are shown. In parental HEK293 cells without GIRK4 expression, the GIRK4 antibody does not stain the cells. Incubation of the M2 GIRK1/4 cell line with only the secondary antibody and not the primary does not lead to staining

    Article Snippet: GIRK4 (APC-027, Alomone Laboratories) and M2 receptor (AMR-002, Alomone Laboratories) antibodies were used at a final dilution of 1/500.

    Techniques: Immunostaining, Staining, Expressing, Incubation

    K + current activated by carbachol in HEK293 stable cell line. Carbachol activates an inwardly rectifying K + current in a stable HEK293 cell line expressing the M2 receptor and the GIRK1 and GIRK4 channel subunits. Sample voltage-clamp recordings and current voltage relationships are shown

    Journal: Pflugers Archiv

    Article Title: HL-1 cells express an inwardly rectifying K+ current activated via muscarinic receptors comparable to that in mouse atrial myocytes

    doi: 10.1007/s00424-010-0799-z

    Figure Lengend Snippet: K + current activated by carbachol in HEK293 stable cell line. Carbachol activates an inwardly rectifying K + current in a stable HEK293 cell line expressing the M2 receptor and the GIRK1 and GIRK4 channel subunits. Sample voltage-clamp recordings and current voltage relationships are shown

    Article Snippet: GIRK4 (APC-027, Alomone Laboratories) and M2 receptor (AMR-002, Alomone Laboratories) antibodies were used at a final dilution of 1/500.

    Techniques: Stable Transfection, Expressing

    Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P

    Journal: International Journal of Molecular Medicine

    Article Title: Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling

    doi: 10.3892/ijmm.2018.3960

    Figure Lengend Snippet: Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P

    Article Snippet: Following the separation of total proteins (30 μ g) by 4-20% SDS-PAGE, these proteins were then transferred to nitrocellulose membranes for 2-3 h at 40 V. Subsequently, the membranes were incubated with primary antibodies targeting mAChR M2 (cat. no. AMR-002; 1:1,000; Alomone Labs, Jerusalem, Israel), mAChR M3 (1:1,000, cat. no. AMR-006; Alomone Labs), phosphoinositide 3-kinase (PI3K, 1:1,000, cat. no. 4292S; Cell Signaling Technology, Inc., Danvers, MA, USA), phosphorylated (p-) PI3K (1:1,000, cat. no. 4228S; Cell Signaling Technology, Inc.), protein kinase C (PKC, 1:1,000, cat. no. 2058S; Cell Signaling Technology, Inc.), p-PKC (1:1,000, cat. no. 9376S; Cell Signaling Technology, Inc.), inositol-requiring enzyme (IRE) 1α (1:1,000, cat. no. ab37073; Abcam, Cambridge, UK), IRE1β (1:1,000, cat. no. SC-10511; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), p-IRE1 (1:1,000, cat. no. 48187; Abcam), c-Jun N-terminal kinase (JNK, 1:1,000, cat. no. 9252; Cell Signaling Technology, Inc.), p-JNK (1:1,000, cat. no. 9251; Cell Signaling Technology, Inc.), guanine nucleotide binding protein (G) α (1:1,000, cat. no. ab128900; Abcam), eukaryotic initiation factor (eIF) 2α (1:1,000, cat. no. 9722; Cell Signaling Technology, Inc.), p-eIF2α (1:1,000, cat. no. 9721; Cell Signaling Technology, Inc.) or β-actin (1:3,000, cat. no. A5316; Sigma-Aldrich Co.; Merck KGaA, Darmstadt, Germany) overnight at 4°C.

    Techniques: Expressing, Western Blot, Immunofluorescence, Standard Deviation

    Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P

    Journal: International Journal of Molecular Medicine

    Article Title: Spicatoside A in red Liriope platyphylla displays a laxative effect in a constipation rat model via regulating mAChRs and ER stress signaling

    doi: 10.3892/ijmm.2018.3960

    Figure Lengend Snippet: Expression of key components in the mAChR signaling pathway. The expression levels of (A) mAChR M2, and (B) mAChR M3 and their respective downstream proteins were determined by (a-c) western blot and (d) immunofluorescence assays. Band intensities were evaluated relative to the intensity of the actin bands. Data are presented as mean ± standard deviation of three replicates (n=5-6 rats per treatment group). * P

    Article Snippet: The pretreated sections were then incubated with anti-mAChR M2 (1:200, cat. no. AMR-002; Alomone Labs) or anti-mAChR M3 (1:200, cat. no. AMR-006; Alomone Labs) antibodies diluted 1:1,000 in blocking buffer.

    Techniques: Expressing, Western Blot, Immunofluorescence, Standard Deviation