m1 muscarinic receptor  (Alomone Labs)


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    Alomone Labs m1 muscarinic receptor
    M1 Muscarinic Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    m1 muscarinic receptor  (Alomone Labs)


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    Alomone Labs m1 muscarinic receptor
    M1 Muscarinic Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m1r antibodies  (Alomone Labs)


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    Alomone Labs m1r antibodies
    <t>M1R</t> immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.
    M1r Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    Images

    1) Product Images from "Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice"

    Article Title: Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice

    Journal: IBRO Neuroscience Reports

    doi: 10.1016/j.ibneur.2021.09.005

    M1R immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.
    Figure Legend Snippet: M1R immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.

    Techniques Used: Labeling, Blocking Assay

    Bar graphs showing a significant reduction in M1R immunogold in PL-PFC dendrites of adult mice receiving Δ9-THC (THC) as adolescents. As compared to vehicle (VEH) mice, animals receiving Δ9-THC had a lower mean density of M1R immunogold particles on the plasma membrane (A) and in the cytoplasm (B) of small, but not medium or large sized dendrites of adult mice chronically exposed to Δ9-THC during adolescence. In (C) the reduction in M1R density in small dendrites of Δ9-THC pretreated mice is statistically significant only when including the total of both plasmalemmal and cytoplasmic labeling ( p < 0.05; Welch’s correction for unequal variance). Values on top of the bars depict absolute number of dendrites in each category.
    Figure Legend Snippet: Bar graphs showing a significant reduction in M1R immunogold in PL-PFC dendrites of adult mice receiving Δ9-THC (THC) as adolescents. As compared to vehicle (VEH) mice, animals receiving Δ9-THC had a lower mean density of M1R immunogold particles on the plasma membrane (A) and in the cytoplasm (B) of small, but not medium or large sized dendrites of adult mice chronically exposed to Δ9-THC during adolescence. In (C) the reduction in M1R density in small dendrites of Δ9-THC pretreated mice is statistically significant only when including the total of both plasmalemmal and cytoplasmic labeling ( p < 0.05; Welch’s correction for unequal variance). Values on top of the bars depict absolute number of dendrites in each category.

    Techniques Used: Labeling

    Separable locations of M1R immunogold and CB1R-immunoperoxidase labeling at axo-spinous synapses in the PL-PFC of adult male mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. In A, M1R immunogold is located on (arrowhead) and near the extrasynaptic plasmalemma of a small dendritic spine (M1-Sp) that receives excitatory-type input from an unlabeled axon terminal (U-Te). Panel B shows M1R immunogold in the cytoplasm near endomembranes (em) beneath an asymmetric synapse formed by an unlabeled axon terminal. M1R gold is seen in axon terminals forming asymmetric synapses with large dendritic spine heads containing M1R immunogold particles (M1-Sp in C) or without immunoreactivity (U-Sp in D). In the upper right corner of panel C, a CB1R peroxidase labeled terminal apposes an axospinous synapse between unlabeled profiles. Small arrows = cytoplasmic M1R immunogold; curved arrows = asymmetric synapses; U-Te = unlabeled axon terminal (U-Te). Scale bar = 500 nm.
    Figure Legend Snippet: Separable locations of M1R immunogold and CB1R-immunoperoxidase labeling at axo-spinous synapses in the PL-PFC of adult male mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. In A, M1R immunogold is located on (arrowhead) and near the extrasynaptic plasmalemma of a small dendritic spine (M1-Sp) that receives excitatory-type input from an unlabeled axon terminal (U-Te). Panel B shows M1R immunogold in the cytoplasm near endomembranes (em) beneath an asymmetric synapse formed by an unlabeled axon terminal. M1R gold is seen in axon terminals forming asymmetric synapses with large dendritic spine heads containing M1R immunogold particles (M1-Sp in C) or without immunoreactivity (U-Sp in D). In the upper right corner of panel C, a CB1R peroxidase labeled terminal apposes an axospinous synapse between unlabeled profiles. Small arrows = cytoplasmic M1R immunogold; curved arrows = asymmetric synapses; U-Te = unlabeled axon terminal (U-Te). Scale bar = 500 nm.

    Techniques Used: Labeling

     M1R-labeled  axon terminals forming either symmetric or asymmetric synapses with dendritic profiles defined by their expression of M1R in the PL-PFC of adult male mice pre-exposed to vehicle or Δ9-THC during adolescence.
    Figure Legend Snippet: M1R-labeled axon terminals forming either symmetric or asymmetric synapses with dendritic profiles defined by their expression of M1R in the PL-PFC of adult male mice pre-exposed to vehicle or Δ9-THC during adolescence.

    Techniques Used: Expressing

    Subcellular localization of M1R immunogold and CB1R-immunoperoxidase labeling at axo-dendritic synapses in the PL-PFC of adult mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. Panel A shows a dually labeled axon terminal (Du-Te) with one M1R gold particle on the presynaptic membrane of an axon terminal containing peroxidase immunoreactivity for CB1R and one large mitochondrion (mt). The symmetry (block arrow) of this inhibitory-type junction contrasts with the thickened asymmetric synapse (curved arrow) formed by an unlabeled terminal (U-Te) convergent on the same unlabeled dendritic profile (UDe). Panel B shows a similar convergent labeling of a single CB1R-immunoreactive terminal and an excitatory-type synapse on a dendrite (M1R-De) in which the M1R gold is aligned on outer membranes contacted by an unlabeled terminal. Cytoplasmic M1R immunogold is associated with a mitochondrion (mt) and on a multivesicular body (mvb) in B and C, respectively. In D, M1R immunogold is localized to endomembranes (em) near the plasma membrane in a dendritic profile (M1-De 1 ) and to the perisynaptic plasma membrane in a second dendrite (M1-De 2 ). One gold particle (arrowhead) opposes the membrane specialization of an asymmetric synapse formed by an unlabeled axon terminal. This dendrite also receives convergent input from an axon terminal containing CB1R peroxidase reaction product. Cytoplasmic M1R gold is shown by small black arrows in all images. Curved arrows = asymmetric synapses; white block arrow = symmetric synapses. Scale bar = 500 nm.
    Figure Legend Snippet: Subcellular localization of M1R immunogold and CB1R-immunoperoxidase labeling at axo-dendritic synapses in the PL-PFC of adult mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. Panel A shows a dually labeled axon terminal (Du-Te) with one M1R gold particle on the presynaptic membrane of an axon terminal containing peroxidase immunoreactivity for CB1R and one large mitochondrion (mt). The symmetry (block arrow) of this inhibitory-type junction contrasts with the thickened asymmetric synapse (curved arrow) formed by an unlabeled terminal (U-Te) convergent on the same unlabeled dendritic profile (UDe). Panel B shows a similar convergent labeling of a single CB1R-immunoreactive terminal and an excitatory-type synapse on a dendrite (M1R-De) in which the M1R gold is aligned on outer membranes contacted by an unlabeled terminal. Cytoplasmic M1R immunogold is associated with a mitochondrion (mt) and on a multivesicular body (mvb) in B and C, respectively. In D, M1R immunogold is localized to endomembranes (em) near the plasma membrane in a dendritic profile (M1-De 1 ) and to the perisynaptic plasma membrane in a second dendrite (M1-De 2 ). One gold particle (arrowhead) opposes the membrane specialization of an asymmetric synapse formed by an unlabeled axon terminal. This dendrite also receives convergent input from an axon terminal containing CB1R peroxidase reaction product. Cytoplasmic M1R gold is shown by small black arrows in all images. Curved arrows = asymmetric synapses; white block arrow = symmetric synapses. Scale bar = 500 nm.

    Techniques Used: Labeling, Blocking Assay

    CB1R-terminals forming symmetric or asymmetric synapses with  M1R-labeled  or unlabeled dendritic profiles in the PL-PFC of adult mice that received vehicle or Δ9-THC during adolescence.
    Figure Legend Snippet: CB1R-terminals forming symmetric or asymmetric synapses with M1R-labeled or unlabeled dendritic profiles in the PL-PFC of adult mice that received vehicle or Δ9-THC during adolescence.

    Techniques Used:

    anti chrm1 antibody  (Alomone Labs)


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    Alomone Labs anti chrm1 antibody
    Anti Chrm1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mab alomone  (Alomone Labs)


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    Alomone Labs mab alomone
    Mab Alomone, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human m1achr  (Alomone Labs)


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    Alomone Labs human m1achr
    Human M1achr, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human m1achr  (Alomone Labs)


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    Alomone Labs human m1achr
    (a) shows the percentage of the calbindin-D28k (CB) and calretinin (CR) immunoreactive populations that are also immunoreactive (ir) for the m1 acetylcholine receptor <t>(m1AChR),</t> collapsed across all cortical layers. 55% of CB-ir neurons express m1AChRs, while 10% of CR-ir neurons express m1AChRs.(b)shows the same data (52% of CB-ir neurons and 11% of CR-ir neurons), but for layers II and III only.(c)shows the percentage of cells immunoreactive for m1AChRs that express CB or CR collapsed across all cortical layers. 2% of m1-ir neurons express CB, while 0.5% of m1-ir cells express CR. (d) shows the samedata (4% of m1AChR-ir neurons express CB, while 1% express CR), but for layers II and III only. Bars indicate standard error and asterisks indicate statistical significance (p<0.01).
    Human M1achr, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Most calbindin-immunoreactive neurons, but few calretinin-immunoreative neurons, express the m1 acetylcholine receptor in the middle temporal visual area of the macaque monkey"

    Article Title: Most calbindin-immunoreactive neurons, but few calretinin-immunoreative neurons, express the m1 acetylcholine receptor in the middle temporal visual area of the macaque monkey

    Journal: bioRxiv

    doi: 10.1101/271924

    (a) shows the percentage of the calbindin-D28k (CB) and calretinin (CR) immunoreactive populations that are also immunoreactive (ir) for the m1 acetylcholine receptor (m1AChR), collapsed across all cortical layers. 55% of CB-ir neurons express m1AChRs, while 10% of CR-ir neurons express m1AChRs.(b)shows the same data (52% of CB-ir neurons and 11% of CR-ir neurons), but for layers II and III only.(c)shows the percentage of cells immunoreactive for m1AChRs that express CB or CR collapsed across all cortical layers. 2% of m1-ir neurons express CB, while 0.5% of m1-ir cells express CR. (d) shows the samedata (4% of m1AChR-ir neurons express CB, while 1% express CR), but for layers II and III only. Bars indicate standard error and asterisks indicate statistical significance (p<0.01).
    Figure Legend Snippet: (a) shows the percentage of the calbindin-D28k (CB) and calretinin (CR) immunoreactive populations that are also immunoreactive (ir) for the m1 acetylcholine receptor (m1AChR), collapsed across all cortical layers. 55% of CB-ir neurons express m1AChRs, while 10% of CR-ir neurons express m1AChRs.(b)shows the same data (52% of CB-ir neurons and 11% of CR-ir neurons), but for layers II and III only.(c)shows the percentage of cells immunoreactive for m1AChRs that express CB or CR collapsed across all cortical layers. 2% of m1-ir neurons express CB, while 0.5% of m1-ir cells express CR. (d) shows the samedata (4% of m1AChR-ir neurons express CB, while 1% express CR), but for layers II and III only. Bars indicate standard error and asterisks indicate statistical significance (p<0.01).

    Techniques Used:

    Comparison of the m1 acetylcholine receptor (m1AChR) expression by populations immunoreactive for calretinin (CR), calbindin-D28k (CB), and parvalbumin (PV) in primary visual area V1 (dark gray) and middle temporal area MT (light gray). In V1, 40% of CR-immunoreactive neurons, 60% of CB-immunoreactive neurons, and 80% of PV-immunoreactive neurons express m1AChR. Those numbers in MT are 10%, 55%, and 75%, respectively.
    Figure Legend Snippet: Comparison of the m1 acetylcholine receptor (m1AChR) expression by populations immunoreactive for calretinin (CR), calbindin-D28k (CB), and parvalbumin (PV) in primary visual area V1 (dark gray) and middle temporal area MT (light gray). In V1, 40% of CR-immunoreactive neurons, 60% of CB-immunoreactive neurons, and 80% of PV-immunoreactive neurons express m1AChR. Those numbers in MT are 10%, 55%, and 75%, respectively.

    Techniques Used: Expressing

    muscarinic m1 receptor  (Alomone Labs)


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    Alomone Labs muscarinic m1 receptor
    Muscarinic M1 Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti m 1  (Alomone Labs)


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    Alomone Labs anti m 1
    Detection of M 1 -M 5 receptors by means of immunofluorescence in BMVECs and bEnd.3 cells. The labeling obtained with anti-M 1–5 antibodies (green) is here merged with TO-PRO ® -3 nuclear staining (blue); scale bar: 10 µm. The table shows qualitative estimates of protein abundance in the plasma membrane (PM), cytoplasm (Cyt), and nucleus (N). The evaluation was based on visual assessment of green fluorescence intensity on 40 cells per antibody, scored from not observable (−) to very intense (+++).
    Anti M 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "All muscarinic acetylcholine receptors (M 1 -M 5 ) are expressed in murine brain microvascular endothelium"

    Article Title: All muscarinic acetylcholine receptors (M 1 -M 5 ) are expressed in murine brain microvascular endothelium

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-05384-z

    Detection of M 1 -M 5 receptors by means of immunofluorescence in BMVECs and bEnd.3 cells. The labeling obtained with anti-M 1–5 antibodies (green) is here merged with TO-PRO ® -3 nuclear staining (blue); scale bar: 10 µm. The table shows qualitative estimates of protein abundance in the plasma membrane (PM), cytoplasm (Cyt), and nucleus (N). The evaluation was based on visual assessment of green fluorescence intensity on 40 cells per antibody, scored from not observable (−) to very intense (+++).
    Figure Legend Snippet: Detection of M 1 -M 5 receptors by means of immunofluorescence in BMVECs and bEnd.3 cells. The labeling obtained with anti-M 1–5 antibodies (green) is here merged with TO-PRO ® -3 nuclear staining (blue); scale bar: 10 µm. The table shows qualitative estimates of protein abundance in the plasma membrane (PM), cytoplasm (Cyt), and nucleus (N). The evaluation was based on visual assessment of green fluorescence intensity on 40 cells per antibody, scored from not observable (−) to very intense (+++).

    Techniques Used: Immunofluorescence, Labeling, Staining, Fluorescence

    m 1 r gfp fusion protein  (Alomone Labs)


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    Alomone Labs m 1 r gfp fusion protein
    M 1 R Gfp Fusion Protein, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti m1 antibody  (Alomone Labs)


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    Alomone Labs rabbit anti m1 antibody
    Rabbit Anti M1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs m1 muscarinic receptor
    M1 Muscarinic Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs m1r antibodies
    <t>M1R</t> immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.
    M1r Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs anti chrm1 antibody
    <t>M1R</t> immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.
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    Alomone Labs mab alomone
    <t>M1R</t> immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.
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    <t>M1R</t> immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.
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    Alomone Labs muscarinic m1 receptor
    <t>M1R</t> immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.
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    Alomone Labs anti m 1
    Detection of M 1 -M 5 receptors by means of immunofluorescence in BMVECs and bEnd.3 cells. The labeling obtained with anti-M 1–5 antibodies (green) is here merged with TO-PRO ® -3 nuclear staining (blue); scale bar: 10 µm. The table shows qualitative estimates of protein abundance in the plasma membrane (PM), cytoplasm (Cyt), and nucleus (N). The evaluation was based on visual assessment of green fluorescence intensity on 40 cells per antibody, scored from not observable (−) to very intense (+++).
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    Alomone Labs m 1 r gfp fusion protein
    Detection of M 1 -M 5 receptors by means of immunofluorescence in BMVECs and bEnd.3 cells. The labeling obtained with anti-M 1–5 antibodies (green) is here merged with TO-PRO ® -3 nuclear staining (blue); scale bar: 10 µm. The table shows qualitative estimates of protein abundance in the plasma membrane (PM), cytoplasm (Cyt), and nucleus (N). The evaluation was based on visual assessment of green fluorescence intensity on 40 cells per antibody, scored from not observable (−) to very intense (+++).
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    Alomone Labs rabbit anti m1 antibody
    Detection of M 1 -M 5 receptors by means of immunofluorescence in BMVECs and bEnd.3 cells. The labeling obtained with anti-M 1–5 antibodies (green) is here merged with TO-PRO ® -3 nuclear staining (blue); scale bar: 10 µm. The table shows qualitative estimates of protein abundance in the plasma membrane (PM), cytoplasm (Cyt), and nucleus (N). The evaluation was based on visual assessment of green fluorescence intensity on 40 cells per antibody, scored from not observable (−) to very intense (+++).
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    Image Search Results


    M1R immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.

    Journal: IBRO Neuroscience Reports

    Article Title: Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice

    doi: 10.1016/j.ibneur.2021.09.005

    Figure Lengend Snippet: M1R immunogold in the cytoplasm of small dendritic shafts receiving synaptic input from unlabeled (A–E) and CB1R immunoperoxidase labeled (B–F) axon terminals within the PL-PFC of adult male mice. These mice received 14 consecutive daily injections of vehicle (A,B black border) or ∆9-THC (C,E no border) through adolescence. Panel A shows, M1R immunogold (small arrows) in the cytoplasm and in contact with the perisynaptic plasma membrane contacted by an unlabeled terminal forming a symmetric synapse (block arrows) like the junction formed by a CB1-labeled terminal in B. Panels C-D and E-F respectively show unlabeled and CB1R-labeled terminals presynaptic to dendritic profiles with a mainly cytoplasmic distribution of M1R gold particles in the PL-PFC of mice that received ∆9-THC during adolescence. M1R-De = Immunogold-labeled dendrite; CB1R-Te = immunoperoxidase CB1R-labeled axon terminal; white block arrows = symmetric synapses; U-Ax = unlabeled axon; CB1-Ax = CB1R-labeled axon; curved black arrows = asymmetric excitatory-type synapses; small black arrows = cytoplasmic M1R gold particles; white arrowheads = perisynaptic plasmalemmal M1R immunogold; U-Te = unlabeled terminal; U-mvb = unlabeled multivesicular body; mt = mitochondrion = Scale bar = 500 nm.

    Article Snippet: These sections were incubated for 24 h at room temperature in a solution containing both CB1R and M1R antibodies (Alomone Labs, Jerusalem, Israel Cat # AMR-001).

    Techniques: Labeling, Blocking Assay

    Bar graphs showing a significant reduction in M1R immunogold in PL-PFC dendrites of adult mice receiving Δ9-THC (THC) as adolescents. As compared to vehicle (VEH) mice, animals receiving Δ9-THC had a lower mean density of M1R immunogold particles on the plasma membrane (A) and in the cytoplasm (B) of small, but not medium or large sized dendrites of adult mice chronically exposed to Δ9-THC during adolescence. In (C) the reduction in M1R density in small dendrites of Δ9-THC pretreated mice is statistically significant only when including the total of both plasmalemmal and cytoplasmic labeling ( p < 0.05; Welch’s correction for unequal variance). Values on top of the bars depict absolute number of dendrites in each category.

    Journal: IBRO Neuroscience Reports

    Article Title: Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice

    doi: 10.1016/j.ibneur.2021.09.005

    Figure Lengend Snippet: Bar graphs showing a significant reduction in M1R immunogold in PL-PFC dendrites of adult mice receiving Δ9-THC (THC) as adolescents. As compared to vehicle (VEH) mice, animals receiving Δ9-THC had a lower mean density of M1R immunogold particles on the plasma membrane (A) and in the cytoplasm (B) of small, but not medium or large sized dendrites of adult mice chronically exposed to Δ9-THC during adolescence. In (C) the reduction in M1R density in small dendrites of Δ9-THC pretreated mice is statistically significant only when including the total of both plasmalemmal and cytoplasmic labeling ( p < 0.05; Welch’s correction for unequal variance). Values on top of the bars depict absolute number of dendrites in each category.

    Article Snippet: These sections were incubated for 24 h at room temperature in a solution containing both CB1R and M1R antibodies (Alomone Labs, Jerusalem, Israel Cat # AMR-001).

    Techniques: Labeling

    Separable locations of M1R immunogold and CB1R-immunoperoxidase labeling at axo-spinous synapses in the PL-PFC of adult male mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. In A, M1R immunogold is located on (arrowhead) and near the extrasynaptic plasmalemma of a small dendritic spine (M1-Sp) that receives excitatory-type input from an unlabeled axon terminal (U-Te). Panel B shows M1R immunogold in the cytoplasm near endomembranes (em) beneath an asymmetric synapse formed by an unlabeled axon terminal. M1R gold is seen in axon terminals forming asymmetric synapses with large dendritic spine heads containing M1R immunogold particles (M1-Sp in C) or without immunoreactivity (U-Sp in D). In the upper right corner of panel C, a CB1R peroxidase labeled terminal apposes an axospinous synapse between unlabeled profiles. Small arrows = cytoplasmic M1R immunogold; curved arrows = asymmetric synapses; U-Te = unlabeled axon terminal (U-Te). Scale bar = 500 nm.

    Journal: IBRO Neuroscience Reports

    Article Title: Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice

    doi: 10.1016/j.ibneur.2021.09.005

    Figure Lengend Snippet: Separable locations of M1R immunogold and CB1R-immunoperoxidase labeling at axo-spinous synapses in the PL-PFC of adult male mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. In A, M1R immunogold is located on (arrowhead) and near the extrasynaptic plasmalemma of a small dendritic spine (M1-Sp) that receives excitatory-type input from an unlabeled axon terminal (U-Te). Panel B shows M1R immunogold in the cytoplasm near endomembranes (em) beneath an asymmetric synapse formed by an unlabeled axon terminal. M1R gold is seen in axon terminals forming asymmetric synapses with large dendritic spine heads containing M1R immunogold particles (M1-Sp in C) or without immunoreactivity (U-Sp in D). In the upper right corner of panel C, a CB1R peroxidase labeled terminal apposes an axospinous synapse between unlabeled profiles. Small arrows = cytoplasmic M1R immunogold; curved arrows = asymmetric synapses; U-Te = unlabeled axon terminal (U-Te). Scale bar = 500 nm.

    Article Snippet: These sections were incubated for 24 h at room temperature in a solution containing both CB1R and M1R antibodies (Alomone Labs, Jerusalem, Israel Cat # AMR-001).

    Techniques: Labeling

     M1R-labeled  axon terminals forming either symmetric or asymmetric synapses with dendritic profiles defined by their expression of M1R in the PL-PFC of adult male mice pre-exposed to vehicle or Δ9-THC during adolescence.

    Journal: IBRO Neuroscience Reports

    Article Title: Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice

    doi: 10.1016/j.ibneur.2021.09.005

    Figure Lengend Snippet: M1R-labeled axon terminals forming either symmetric or asymmetric synapses with dendritic profiles defined by their expression of M1R in the PL-PFC of adult male mice pre-exposed to vehicle or Δ9-THC during adolescence.

    Article Snippet: These sections were incubated for 24 h at room temperature in a solution containing both CB1R and M1R antibodies (Alomone Labs, Jerusalem, Israel Cat # AMR-001).

    Techniques: Expressing

    Subcellular localization of M1R immunogold and CB1R-immunoperoxidase labeling at axo-dendritic synapses in the PL-PFC of adult mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. Panel A shows a dually labeled axon terminal (Du-Te) with one M1R gold particle on the presynaptic membrane of an axon terminal containing peroxidase immunoreactivity for CB1R and one large mitochondrion (mt). The symmetry (block arrow) of this inhibitory-type junction contrasts with the thickened asymmetric synapse (curved arrow) formed by an unlabeled terminal (U-Te) convergent on the same unlabeled dendritic profile (UDe). Panel B shows a similar convergent labeling of a single CB1R-immunoreactive terminal and an excitatory-type synapse on a dendrite (M1R-De) in which the M1R gold is aligned on outer membranes contacted by an unlabeled terminal. Cytoplasmic M1R immunogold is associated with a mitochondrion (mt) and on a multivesicular body (mvb) in B and C, respectively. In D, M1R immunogold is localized to endomembranes (em) near the plasma membrane in a dendritic profile (M1-De 1 ) and to the perisynaptic plasma membrane in a second dendrite (M1-De 2 ). One gold particle (arrowhead) opposes the membrane specialization of an asymmetric synapse formed by an unlabeled axon terminal. This dendrite also receives convergent input from an axon terminal containing CB1R peroxidase reaction product. Cytoplasmic M1R gold is shown by small black arrows in all images. Curved arrows = asymmetric synapses; white block arrow = symmetric synapses. Scale bar = 500 nm.

    Journal: IBRO Neuroscience Reports

    Article Title: Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice

    doi: 10.1016/j.ibneur.2021.09.005

    Figure Lengend Snippet: Subcellular localization of M1R immunogold and CB1R-immunoperoxidase labeling at axo-dendritic synapses in the PL-PFC of adult mice receiving vehicle (A) or Δ9-THC (B–D) as adolescents. Panel A shows a dually labeled axon terminal (Du-Te) with one M1R gold particle on the presynaptic membrane of an axon terminal containing peroxidase immunoreactivity for CB1R and one large mitochondrion (mt). The symmetry (block arrow) of this inhibitory-type junction contrasts with the thickened asymmetric synapse (curved arrow) formed by an unlabeled terminal (U-Te) convergent on the same unlabeled dendritic profile (UDe). Panel B shows a similar convergent labeling of a single CB1R-immunoreactive terminal and an excitatory-type synapse on a dendrite (M1R-De) in which the M1R gold is aligned on outer membranes contacted by an unlabeled terminal. Cytoplasmic M1R immunogold is associated with a mitochondrion (mt) and on a multivesicular body (mvb) in B and C, respectively. In D, M1R immunogold is localized to endomembranes (em) near the plasma membrane in a dendritic profile (M1-De 1 ) and to the perisynaptic plasma membrane in a second dendrite (M1-De 2 ). One gold particle (arrowhead) opposes the membrane specialization of an asymmetric synapse formed by an unlabeled axon terminal. This dendrite also receives convergent input from an axon terminal containing CB1R peroxidase reaction product. Cytoplasmic M1R gold is shown by small black arrows in all images. Curved arrows = asymmetric synapses; white block arrow = symmetric synapses. Scale bar = 500 nm.

    Article Snippet: These sections were incubated for 24 h at room temperature in a solution containing both CB1R and M1R antibodies (Alomone Labs, Jerusalem, Israel Cat # AMR-001).

    Techniques: Labeling, Blocking Assay

    CB1R-terminals forming symmetric or asymmetric synapses with  M1R-labeled  or unlabeled dendritic profiles in the PL-PFC of adult mice that received vehicle or Δ9-THC during adolescence.

    Journal: IBRO Neuroscience Reports

    Article Title: Adolescent administration of Δ 9 -THC decreases the expression and function of muscarinic-1 receptors in prelimbic prefrontal cortical neurons of adult male mice

    doi: 10.1016/j.ibneur.2021.09.005

    Figure Lengend Snippet: CB1R-terminals forming symmetric or asymmetric synapses with M1R-labeled or unlabeled dendritic profiles in the PL-PFC of adult mice that received vehicle or Δ9-THC during adolescence.

    Article Snippet: These sections were incubated for 24 h at room temperature in a solution containing both CB1R and M1R antibodies (Alomone Labs, Jerusalem, Israel Cat # AMR-001).

    Techniques:

    Detection of M 1 -M 5 receptors by means of immunofluorescence in BMVECs and bEnd.3 cells. The labeling obtained with anti-M 1–5 antibodies (green) is here merged with TO-PRO ® -3 nuclear staining (blue); scale bar: 10 µm. The table shows qualitative estimates of protein abundance in the plasma membrane (PM), cytoplasm (Cyt), and nucleus (N). The evaluation was based on visual assessment of green fluorescence intensity on 40 cells per antibody, scored from not observable (−) to very intense (+++).

    Journal: Scientific Reports

    Article Title: All muscarinic acetylcholine receptors (M 1 -M 5 ) are expressed in murine brain microvascular endothelium

    doi: 10.1038/s41598-017-05384-z

    Figure Lengend Snippet: Detection of M 1 -M 5 receptors by means of immunofluorescence in BMVECs and bEnd.3 cells. The labeling obtained with anti-M 1–5 antibodies (green) is here merged with TO-PRO ® -3 nuclear staining (blue); scale bar: 10 µm. The table shows qualitative estimates of protein abundance in the plasma membrane (PM), cytoplasm (Cyt), and nucleus (N). The evaluation was based on visual assessment of green fluorescence intensity on 40 cells per antibody, scored from not observable (−) to very intense (+++).

    Article Snippet: The following polyclonal rabbit primary antibodies from Alomone, Israel were used: anti-M 1 (1:100, #AMR-001), anti-M 2 (1:100, #AMR-002), anti-M 3 (1:100, #AMR-006), anti-M 4 (1:100, #AMR-004), and anti-M 5 (1:100, #AMR-005).

    Techniques: Immunofluorescence, Labeling, Staining, Fluorescence