anti kir3 1  (Alomone Labs)


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    Name:
    Mouse Anti GIRK1 Kir3 1 extracellular Antibody
    Description:
    Mouse Anti GIRK1 Kir3 1 extracellular Antibody ALM 031 is a highly specific mouse monoclonal antibody directed against an epitope of the human protein The antibody can be used in western blot and immunocytochemistry applications It has been designed to recognize Kir3 1 from human rat and mouse samples
    Catalog Number:
    ALM-031
    Price:
    360.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified from cultured hybridoma medium.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcg
    Antibody Type:
    Monoclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Mouse
    Isotype:
    IgG1
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    Structured Review

    Alomone Labs anti kir3 1
    Mouse Anti GIRK1 Kir3 1 extracellular Antibody
    Mouse Anti GIRK1 Kir3 1 extracellular Antibody ALM 031 is a highly specific mouse monoclonal antibody directed against an epitope of the human protein The antibody can be used in western blot and immunocytochemistry applications It has been designed to recognize Kir3 1 from human rat and mouse samples
    https://www.bioz.com/result/anti kir3 1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kir3 1 - by Bioz Stars, 2021-09
    93/100 stars

    Images

    1) Product Images from "G-Protein-Gated Potassium Channels Containing Kir3.2 and Kir3.3 Subunits Mediate the Acute Inhibitory Effects of Opioids on Locus Ceruleus Neurons"

    Article Title: G-Protein-Gated Potassium Channels Containing Kir3.2 and Kir3.3 Subunits Mediate the Acute Inhibitory Effects of Opioids on Locus Ceruleus Neurons

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.22-11-04328.2002

    Kir3 protein levels in wild-type and knock-out mouse brains. Membrane proteins from wild-type ( WT ), Kir3.2 knock-out ( 2 ko ), Kir3.3 knock-out ( 3 ko ), and Kir3.2/3.3 double knock-out ( 2/3 ko ) mouse brains were electrophoresed and probed using anti-Kir3 antibodies. Loading consistency was evaluated using an anti-NMDA receptor subunit ( NR1 ). Note that the heavily glycosylated form of Kir3.1 is most affected by the ablation of Kir3.2 and/or Kir3.3. Ab , Primary antibody.
    Figure Legend Snippet: Kir3 protein levels in wild-type and knock-out mouse brains. Membrane proteins from wild-type ( WT ), Kir3.2 knock-out ( 2 ko ), Kir3.3 knock-out ( 3 ko ), and Kir3.2/3.3 double knock-out ( 2/3 ko ) mouse brains were electrophoresed and probed using anti-Kir3 antibodies. Loading consistency was evaluated using an anti-NMDA receptor subunit ( NR1 ). Note that the heavily glycosylated form of Kir3.1 is most affected by the ablation of Kir3.2 and/or Kir3.3. Ab , Primary antibody.

    Techniques Used: Knock-Out

    2) Product Images from "Identification of a Kir3.4 Mutation in Congenital Long QT Syndrome"

    Article Title: Identification of a Kir3.4 Mutation in Congenital Long QT Syndrome

    Journal: American Journal of Human Genetics

    doi: 10.1016/j.ajhg.2010.04.017

    Cell Surface Expression of Kir3.4-Gly387Arg and Kir3.1 Western blotting of HEK293 cells transiently cotransfected with hKir3.1 and hKir3.4 or hKir3.4-Gly387Arg. Note: G387R in this figure is equivalent to Gly387Arg. (A) Representative exposures of biotinylated proteins and unbound protein fractions with the indicated antibodies (AB). For mock-transfected HEK293 cells, whole-cell lysate was loaded. Na + /K+-ATPase α1 served as a loading control to make sure that equal amounts of cell lysates were loaded onto the gel. Calnexin, which is an endoplasmic-reticulum-resident protein, served as control for selective biotinylation of plasma membrane proteins. (B) Average ± SEM values from quantification of six independent experiments. ∗ p
    Figure Legend Snippet: Cell Surface Expression of Kir3.4-Gly387Arg and Kir3.1 Western blotting of HEK293 cells transiently cotransfected with hKir3.1 and hKir3.4 or hKir3.4-Gly387Arg. Note: G387R in this figure is equivalent to Gly387Arg. (A) Representative exposures of biotinylated proteins and unbound protein fractions with the indicated antibodies (AB). For mock-transfected HEK293 cells, whole-cell lysate was loaded. Na + /K+-ATPase α1 served as a loading control to make sure that equal amounts of cell lysates were loaded onto the gel. Calnexin, which is an endoplasmic-reticulum-resident protein, served as control for selective biotinylation of plasma membrane proteins. (B) Average ± SEM values from quantification of six independent experiments. ∗ p

    Techniques Used: Expressing, Western Blot, Transfection

    Human Atrial and Ventricular Expression of Kir3.1 and Kir3.4 (A) Western blotting of proteins extracted from tissue from the left ventricular endocardial free wall (LV) and the lateral wall of the right atrium (RA) from four patients (e.g., patient 1: LV1 and RA1). (B and C) Quantification of expression levels of Kir3.1 (B) and Kir3.4 (C) in the atria and ventricles. Average ± SEM arbitrary quantification values for atrial versus ventricular expression were as follows: Kir3.1, 237 ± 54 versus 62 ± 7; Kir3.4, 182 ± 5 versus 82 ± 4. ∗ p
    Figure Legend Snippet: Human Atrial and Ventricular Expression of Kir3.1 and Kir3.4 (A) Western blotting of proteins extracted from tissue from the left ventricular endocardial free wall (LV) and the lateral wall of the right atrium (RA) from four patients (e.g., patient 1: LV1 and RA1). (B and C) Quantification of expression levels of Kir3.1 (B) and Kir3.4 (C) in the atria and ventricles. Average ± SEM arbitrary quantification values for atrial versus ventricular expression were as follows: Kir3.1, 237 ± 54 versus 62 ± 7; Kir3.4, 182 ± 5 versus 82 ± 4. ∗ p

    Techniques Used: Expressing, Western Blot

    Related Articles

    Incubation:

    Article Title: Identification of a Kir3.4 Mutation in Congenital Long QT Syndrome
    Article Snippet: .. Proteins were transferred onto Hybond-P polyvinylidene difluoride transfer membranes (Amersham Biosciences, 0.45 μm), and membranes were blocked in 5% nonfat milk in TBST (0.1% Tween 20 in Tris-buffered saline solution) for 2 hr at room temperature and then incubated with primary antibody against Kir3.1 (1:1,000; Alomone Labs), Kir3.4 (1:1,000; Alomone Labs), calnexin (1:2,000; Nordic Biosite), or Na+ /K+-ATPase α1 (1:500; Santa Cruz Biotechnology) overnight. ..

    Generated:

    Article Title: Critical evaluation of KCNJ3 gene product detection in human breast cancer: mRNA in situ hybridisation is superior to immunohistochemistry
    Article Snippet: .. Anti-GIRK1 antibodiesAb#1: mouse monoclonal antibody from Abcam (#Ab11924); Ab#2: rabbit polyclonal antibody directed against the C-terminus of GIRK1 (generated by Kurt Schmidt, Institute of Pharmaceutical Sciences, University of Graz, Austria; described in ref. ); Ab#3: rabbit polyclonal antibody directed against the N-terminus (N-T) of GIRK1 (generated by Kurt Schmidt); Ab#4: polyclonal goat antibody from Santa Cruz (#Sc-16131); Ab#5: rabbit polyclonal antibody from Alomone (#APC-005) and Ab#6: mouse monoclonal antibody from Alomone (#ALM-031). ..

    Expressing:

    Article Title: Atrial Fibrillation-Mediated Upregulation of miR-30d Regulates Myocardial Electrical Remodeling of the G-Protein-Gated K(+) Channel, IK.ACh.
    Article Snippet: .. Immunoblot AnalysisTo measure Kir3.1 and Cav1.2 protein expression, an immunoblot analysis in human tissue or cardiomyocytes with miR-30d mimic was performed by using an anti-Kir3.1 or anti-Cav1.2 antibody (1:1,000; Alomone Labs, Jerusalem, Israel). ..

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  • News
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  • 93
    Alomone Labs anti kir3 1
    Kir3 protein levels in wild-type and knock-out mouse brains. Membrane proteins from wild-type ( WT ), Kir3.2 knock-out ( 2 ko ), Kir3.3 knock-out ( 3 ko ), and Kir3.2/3.3 double knock-out ( 2/3 ko ) mouse brains were electrophoresed and probed using anti-Kir3 antibodies. Loading consistency was evaluated using an anti-NMDA receptor subunit ( NR1 ). Note that the heavily glycosylated form of <t>Kir3.1</t> is most affected by the ablation of Kir3.2 and/or Kir3.3. Ab , Primary antibody.
    Anti Kir3 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kir3 1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kir3 1 - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Kir3 protein levels in wild-type and knock-out mouse brains. Membrane proteins from wild-type ( WT ), Kir3.2 knock-out ( 2 ko ), Kir3.3 knock-out ( 3 ko ), and Kir3.2/3.3 double knock-out ( 2/3 ko ) mouse brains were electrophoresed and probed using anti-Kir3 antibodies. Loading consistency was evaluated using an anti-NMDA receptor subunit ( NR1 ). Note that the heavily glycosylated form of Kir3.1 is most affected by the ablation of Kir3.2 and/or Kir3.3. Ab , Primary antibody.

    Journal: The Journal of Neuroscience

    Article Title: G-Protein-Gated Potassium Channels Containing Kir3.2 and Kir3.3 Subunits Mediate the Acute Inhibitory Effects of Opioids on Locus Ceruleus Neurons

    doi: 10.1523/JNEUROSCI.22-11-04328.2002

    Figure Lengend Snippet: Kir3 protein levels in wild-type and knock-out mouse brains. Membrane proteins from wild-type ( WT ), Kir3.2 knock-out ( 2 ko ), Kir3.3 knock-out ( 3 ko ), and Kir3.2/3.3 double knock-out ( 2/3 ko ) mouse brains were electrophoresed and probed using anti-Kir3 antibodies. Loading consistency was evaluated using an anti-NMDA receptor subunit ( NR1 ). Note that the heavily glycosylated form of Kir3.1 is most affected by the ablation of Kir3.2 and/or Kir3.3. Ab , Primary antibody.

    Article Snippet: Anti-Kir3.1 and Kir3.2 antibodies were purchased (Alomone Labs, Jerusalem, Israel) and used at 1:200 dilutions as specified.

    Techniques: Knock-Out

    Cell Surface Expression of Kir3.4-Gly387Arg and Kir3.1 Western blotting of HEK293 cells transiently cotransfected with hKir3.1 and hKir3.4 or hKir3.4-Gly387Arg. Note: G387R in this figure is equivalent to Gly387Arg. (A) Representative exposures of biotinylated proteins and unbound protein fractions with the indicated antibodies (AB). For mock-transfected HEK293 cells, whole-cell lysate was loaded. Na + /K+-ATPase α1 served as a loading control to make sure that equal amounts of cell lysates were loaded onto the gel. Calnexin, which is an endoplasmic-reticulum-resident protein, served as control for selective biotinylation of plasma membrane proteins. (B) Average ± SEM values from quantification of six independent experiments. ∗ p

    Journal: American Journal of Human Genetics

    Article Title: Identification of a Kir3.4 Mutation in Congenital Long QT Syndrome

    doi: 10.1016/j.ajhg.2010.04.017

    Figure Lengend Snippet: Cell Surface Expression of Kir3.4-Gly387Arg and Kir3.1 Western blotting of HEK293 cells transiently cotransfected with hKir3.1 and hKir3.4 or hKir3.4-Gly387Arg. Note: G387R in this figure is equivalent to Gly387Arg. (A) Representative exposures of biotinylated proteins and unbound protein fractions with the indicated antibodies (AB). For mock-transfected HEK293 cells, whole-cell lysate was loaded. Na + /K+-ATPase α1 served as a loading control to make sure that equal amounts of cell lysates were loaded onto the gel. Calnexin, which is an endoplasmic-reticulum-resident protein, served as control for selective biotinylation of plasma membrane proteins. (B) Average ± SEM values from quantification of six independent experiments. ∗ p

    Article Snippet: Proteins were transferred onto Hybond-P polyvinylidene difluoride transfer membranes (Amersham Biosciences, 0.45 μm), and membranes were blocked in 5% nonfat milk in TBST (0.1% Tween 20 in Tris-buffered saline solution) for 2 hr at room temperature and then incubated with primary antibody against Kir3.1 (1:1,000; Alomone Labs), Kir3.4 (1:1,000; Alomone Labs), calnexin (1:2,000; Nordic Biosite), or Na+ /K+-ATPase α1 (1:500; Santa Cruz Biotechnology) overnight.

    Techniques: Expressing, Western Blot, Transfection

    Human Atrial and Ventricular Expression of Kir3.1 and Kir3.4 (A) Western blotting of proteins extracted from tissue from the left ventricular endocardial free wall (LV) and the lateral wall of the right atrium (RA) from four patients (e.g., patient 1: LV1 and RA1). (B and C) Quantification of expression levels of Kir3.1 (B) and Kir3.4 (C) in the atria and ventricles. Average ± SEM arbitrary quantification values for atrial versus ventricular expression were as follows: Kir3.1, 237 ± 54 versus 62 ± 7; Kir3.4, 182 ± 5 versus 82 ± 4. ∗ p

    Journal: American Journal of Human Genetics

    Article Title: Identification of a Kir3.4 Mutation in Congenital Long QT Syndrome

    doi: 10.1016/j.ajhg.2010.04.017

    Figure Lengend Snippet: Human Atrial and Ventricular Expression of Kir3.1 and Kir3.4 (A) Western blotting of proteins extracted from tissue from the left ventricular endocardial free wall (LV) and the lateral wall of the right atrium (RA) from four patients (e.g., patient 1: LV1 and RA1). (B and C) Quantification of expression levels of Kir3.1 (B) and Kir3.4 (C) in the atria and ventricles. Average ± SEM arbitrary quantification values for atrial versus ventricular expression were as follows: Kir3.1, 237 ± 54 versus 62 ± 7; Kir3.4, 182 ± 5 versus 82 ± 4. ∗ p

    Article Snippet: Proteins were transferred onto Hybond-P polyvinylidene difluoride transfer membranes (Amersham Biosciences, 0.45 μm), and membranes were blocked in 5% nonfat milk in TBST (0.1% Tween 20 in Tris-buffered saline solution) for 2 hr at room temperature and then incubated with primary antibody against Kir3.1 (1:1,000; Alomone Labs), Kir3.4 (1:1,000; Alomone Labs), calnexin (1:2,000; Nordic Biosite), or Na+ /K+-ATPase α1 (1:500; Santa Cruz Biotechnology) overnight.

    Techniques: Expressing, Western Blot