orai1  (Alomone Labs)


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    Name:
    Mouse Anti Human Orai1 extracellular Antibody
    Description:
    Mouse Anti Human Orai1 extracellular Antibody ALM 025 is a highly specific monoclonal antibody directed against an extracellular epitope of the human Orai1 channel The antibody can be used in western blot immunocytochemical and indirect flow cytometry applications and was designed to recognize Orai1 from human samples only
    Catalog Number:
    ALM-025
    Price:
    360.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Indirect Flow Cytometry, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified from cultured hybridoma medium.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcg
    Antibody Type:
    Monoclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Mouse
    Isotype:
    IgM
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    Structured Review

    Alomone Labs orai1
    Mouse Anti Human Orai1 extracellular Antibody
    Mouse Anti Human Orai1 extracellular Antibody ALM 025 is a highly specific monoclonal antibody directed against an extracellular epitope of the human Orai1 channel The antibody can be used in western blot immunocytochemical and indirect flow cytometry applications and was designed to recognize Orai1 from human samples only
    https://www.bioz.com/result/orai1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    orai1 - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Chronic Hypoxia Increases TRPC6 Expression and Basal Intracellular Ca2+ Concentration in Rat Distal Pulmonary Venous Smooth Muscle"

    Article Title: Chronic Hypoxia Increases TRPC6 Expression and Basal Intracellular Ca2+ Concentration in Rat Distal Pulmonary Venous Smooth Muscle

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0112007

    Effects of siOrai1 transfection on basal [Ca 2+ ] i and SOCE in rat PVSMCs. A, B : Western blot showing expression of Orai1 and α-actin protein in rat PVSMC treated with siNT, siOrai1 and transfection vehicle alone (control). E, F : The changes of SOCE in siOrai1, NTsiRNA transfected PVSMCs and transfection vehicle alone treated control. *P
    Figure Legend Snippet: Effects of siOrai1 transfection on basal [Ca 2+ ] i and SOCE in rat PVSMCs. A, B : Western blot showing expression of Orai1 and α-actin protein in rat PVSMC treated with siNT, siOrai1 and transfection vehicle alone (control). E, F : The changes of SOCE in siOrai1, NTsiRNA transfected PVSMCs and transfection vehicle alone treated control. *P

    Techniques Used: Transfection, Western Blot, Expressing

    Related Articles

    Expressing:

    Article Title: Pyridostigmine improves cardiac function and rhythmicity through RyR2 stabilization and inhibition of STIM1‐mediated calcium entry in heart failure, et al. Pyridostigmine improves cardiac function and rhythmicity through RyR2 stabilization and inhibition of STIM1‐mediated calcium entry in heart failure
    Article Snippet: .. For STIM1 (Sigma, #S6072) and Orai1 (Alomone Labs, #ALM‐025), expression was normalized to GAPDH levels. ..

    Incubation:

    Article Title: Hypoxia selectively upregulates cation channels and increases cytosolic [Ca2+] in pulmonary, but not coronary, arterial smooth muscle cells
    Article Snippet: .. Cells were incubated overnight at 4°C with a mouse monoclonal anti-Orai1 antibody (1:100, catalog no. ALM-025, Alomone) ( ) and rabbit polyclonal anti-STIM2 antibody (1:200; catalog no. ab59342, Abcam, Cambridge, MA) ( ). ..

    Concentration Assay:

    Article Title: Generation and characterization of fully human monoclonal antibodies against human Orai1 for autoimmune disease.
    Article Snippet: .. Calcium entry into T cells following antigen stimulation is crucial for nuclear factor of activated T cells (NFAT)-mediated T cell activation.. The movement of calcium is mediated by calcium release-activated calcium (CRAC) channels. ..

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  • News
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  • Bioz Stars
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  • 94
    Alomone Labs orai1
    Effects of siOrai1 transfection on basal [Ca 2+ ] i and SOCE in rat PVSMCs. A, B : Western blot showing expression of <t>Orai1</t> and α-actin protein in rat PVSMC treated with siNT, siOrai1 and transfection vehicle alone (control). E, F : The changes of SOCE in siOrai1, NTsiRNA transfected PVSMCs and transfection vehicle alone treated control. *P
    Orai1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orai1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    orai1 - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Effects of siOrai1 transfection on basal [Ca 2+ ] i and SOCE in rat PVSMCs. A, B : Western blot showing expression of Orai1 and α-actin protein in rat PVSMC treated with siNT, siOrai1 and transfection vehicle alone (control). E, F : The changes of SOCE in siOrai1, NTsiRNA transfected PVSMCs and transfection vehicle alone treated control. *P

    Journal: PLoS ONE

    Article Title: Chronic Hypoxia Increases TRPC6 Expression and Basal Intracellular Ca2+ Concentration in Rat Distal Pulmonary Venous Smooth Muscle

    doi: 10.1371/journal.pone.0112007

    Figure Lengend Snippet: Effects of siOrai1 transfection on basal [Ca 2+ ] i and SOCE in rat PVSMCs. A, B : Western blot showing expression of Orai1 and α-actin protein in rat PVSMC treated with siNT, siOrai1 and transfection vehicle alone (control). E, F : The changes of SOCE in siOrai1, NTsiRNA transfected PVSMCs and transfection vehicle alone treated control. *P

    Article Snippet: These separated proteins were then transferred to polyvinylidene difluoride membranes (pore size 0.45 µm, BioRad) and incubated with rabbit polyclonal antibodies against TRPC1 (Sigma), TRPC6 (Sigma), STIM1 (Cell Signaling) or Orai1 (Alomone labs) for overnight or mouse monoclonal antibody that was specific for α-actin (Sigma) for 1 hour.

    Techniques: Transfection, Western Blot, Expressing

    Hypoxia increases protein level (determined by immunofluorescence intensity) of STIM2 and Orai1 only in PASMC, but not in CASMC. A and B : representative images ( A ) and summarized data ( B , means ± SE) showing STIM2 (green) and Orai1 (red) level in CASMC ( top ) and PASMC ( bottom ) under normoxic (Nor) and hypoxic (Hyp) conditions. The cells were dual-labeled with the anti-STIM2 antibody (green) and Orai1 antibody (red). Nuclei were stained with Hoechst (blue). Experiments were performed in 4 separate immunostaining procedure. ** P

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Hypoxia selectively upregulates cation channels and increases cytosolic [Ca2+] in pulmonary, but not coronary, arterial smooth muscle cells

    doi: 10.1152/ajpcell.00272.2017

    Figure Lengend Snippet: Hypoxia increases protein level (determined by immunofluorescence intensity) of STIM2 and Orai1 only in PASMC, but not in CASMC. A and B : representative images ( A ) and summarized data ( B , means ± SE) showing STIM2 (green) and Orai1 (red) level in CASMC ( top ) and PASMC ( bottom ) under normoxic (Nor) and hypoxic (Hyp) conditions. The cells were dual-labeled with the anti-STIM2 antibody (green) and Orai1 antibody (red). Nuclei were stained with Hoechst (blue). Experiments were performed in 4 separate immunostaining procedure. ** P

    Article Snippet: Cells were incubated overnight at 4°C with a mouse monoclonal anti-Orai1 antibody (1:100, catalog no. ALM-025, Alomone) ( ) and rabbit polyclonal anti-STIM2 antibody (1:200; catalog no. ab59342, Abcam, Cambridge, MA) ( ).

    Techniques: Immunofluorescence, Labeling, Staining, Immunostaining

    Comparison of basal protein expression levels of store-operated Ca 2+ channels (SOCC) (STIM1/2, Orai1/2) and ROCC (TRPC6) and hypoxia-induced changes of protein expression of SOCC (STIM1/2, Orai1/2) and ROCC (TRPC6) in CASMC and PASMC. A and B : representative images ( A ) and summarized data ( B , means ± SE) showing Western blot analysis of STIM1, STIM2, Orai1, Orai2, and TRPC6 in CASMC and PASMC ( n = 5 separate experiments). β-Actin was used as a control. * P

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Hypoxia selectively upregulates cation channels and increases cytosolic [Ca2+] in pulmonary, but not coronary, arterial smooth muscle cells

    doi: 10.1152/ajpcell.00272.2017

    Figure Lengend Snippet: Comparison of basal protein expression levels of store-operated Ca 2+ channels (SOCC) (STIM1/2, Orai1/2) and ROCC (TRPC6) and hypoxia-induced changes of protein expression of SOCC (STIM1/2, Orai1/2) and ROCC (TRPC6) in CASMC and PASMC. A and B : representative images ( A ) and summarized data ( B , means ± SE) showing Western blot analysis of STIM1, STIM2, Orai1, Orai2, and TRPC6 in CASMC and PASMC ( n = 5 separate experiments). β-Actin was used as a control. * P

    Article Snippet: Cells were incubated overnight at 4°C with a mouse monoclonal anti-Orai1 antibody (1:100, catalog no. ALM-025, Alomone) ( ) and rabbit polyclonal anti-STIM2 antibody (1:200; catalog no. ab59342, Abcam, Cambridge, MA) ( ).

    Techniques: Expressing, Western Blot

    Orai1 is ubiquitinated and degraded in a Crbn-dependent manner. a – d The lysates of 293T cells transfected with the indicated plasmids were incubated with anti-FLAG antibody-conjugated agarose beads. Bead-bound proteins were detected with the indicated antibodies. Data are representative of three a , c , d or four b independent experiments. e BMDMs derived from the indicated mice were treated with MG132 (5 µM) for 6 h and then lysed. Proteins in the lysates were detected with the indicated antibodies (top) and the levels of Orai1 were quantified (bottom). n = 3 experiments, mean ± SEM. The numbers in the graph indicate p values. NS not significant (two-way ANOVA).

    Journal: Nature Communications

    Article Title: Crbn modulates calcium influx by regulating Orai1 during efferocytosis

    doi: 10.1038/s41467-020-19272-0

    Figure Lengend Snippet: Orai1 is ubiquitinated and degraded in a Crbn-dependent manner. a – d The lysates of 293T cells transfected with the indicated plasmids were incubated with anti-FLAG antibody-conjugated agarose beads. Bead-bound proteins were detected with the indicated antibodies. Data are representative of three a , c , d or four b independent experiments. e BMDMs derived from the indicated mice were treated with MG132 (5 µM) for 6 h and then lysed. Proteins in the lysates were detected with the indicated antibodies (top) and the levels of Orai1 were quantified (bottom). n = 3 experiments, mean ± SEM. The numbers in the graph indicate p values. NS not significant (two-way ANOVA).

    Article Snippet: Anti-Flag (Sigma, F1804), anti-HA (Santa Cruz, sc-7392), anti-GST (Santa Cruz, sc-138), anti-Crbn (Sigma, HPA045910), anti-Orai1 (Santa Cruz, sc-68895), anti-Orai1 (Alomone labs, ACC-062), anti-Orai2 (Abcam, ab180146), anti-Ampkα (Cell signaling, #2532), anti-phospho-AMPKα (cell signaling, #2535), anti-Stim1 (Abcam, ab108994), anti-Cul4A (Abcam, ab92554), anti-DDB1 (Bethyl Laboratories, A300-462A), anti-phospho-raptor (Cell signaling, #2083), anti-raptor (Cell signaling, #2280), anti-phospho-S6K (Cell signaling, #9206), anti-S6K (Cell signaling, #9202), anti-Lamin B (Santa Cruz, sc-374015), anti-Erk2 (Santa Cruz, sc-154), anti-CD16/32 (BioLegend, #101302), anti-CD11b (BioLegend, #1010207), and anti-β-Actin (Santa Cruz, sc-1616) were purchased.

    Techniques: Transfection, Incubation, Derivative Assay, Mouse Assay

    The interaction of Orai1 with Crbn is attenuated during efferocytosis. a LR73 cells transfected with the indicated plasmids were incubated with TAMRA-stained apoptotic cells for 2 h and analyzed by flow cytometry. n = 3 experiments, mean ± SEM. The numbers in the graph indicate p values. NS not significant (one-way ANOVA). b The experiment was performed as in a . n = 3 experiments, mean ± SEM. NS not significant (two-tailed unpaired Student t test). c LR73 cells transfected with the indicated plasmids were lysed, and the proteins in the lysates were detected by immunoblotting. d BMDMs were incubated with apoptotic cells for the indicated durations were lysed, and the levels of Orai1 were detected with an anti-Orai1 antibody. e LR73 cells transfected with the indicated plasmids were incubated with apoptotic thymocytes for 60 min, and Orai1 ubiquitination were measured. f LR73 cells were incubated with apoptotic thymocytes for the indicated durations and the levels of Crbn were measured. g LR73 cells transfected with the indicated plasmids were incubated with apoptotic thymocytes for the indicated durations, and association between Crbn and Orai1 was measured. h LR73 cells stimulated with TAMRA-stained apoptotic cells were stained with an anti-Crbn antibody and observed using confocal microscopy (left) and Crbn localization was quantified (right). Scale bar, 20 µm. n = 8 cells for control and n = 25 cells for adding apoptotic cells, mean ± SEM. NS not significant (two-tailed unpaired Student t test). i LR73 cells incubated with apoptotic cells were lysed and fractionized. Proteins in the fractions were detected with the indicated antibodies. j LR73 cells transfected with the indicated plasmids were incubated with apoptotic cells, and association between Orai1 and Stim1 was measured. k 293T cells were transfected with the indicated plasmids, and association between Crbn and Oria1 was measured in the presence or absence of Stim1. Data are representative of three c – g , I , k or five j independent experiments. .

    Journal: Nature Communications

    Article Title: Crbn modulates calcium influx by regulating Orai1 during efferocytosis

    doi: 10.1038/s41467-020-19272-0

    Figure Lengend Snippet: The interaction of Orai1 with Crbn is attenuated during efferocytosis. a LR73 cells transfected with the indicated plasmids were incubated with TAMRA-stained apoptotic cells for 2 h and analyzed by flow cytometry. n = 3 experiments, mean ± SEM. The numbers in the graph indicate p values. NS not significant (one-way ANOVA). b The experiment was performed as in a . n = 3 experiments, mean ± SEM. NS not significant (two-tailed unpaired Student t test). c LR73 cells transfected with the indicated plasmids were lysed, and the proteins in the lysates were detected by immunoblotting. d BMDMs were incubated with apoptotic cells for the indicated durations were lysed, and the levels of Orai1 were detected with an anti-Orai1 antibody. e LR73 cells transfected with the indicated plasmids were incubated with apoptotic thymocytes for 60 min, and Orai1 ubiquitination were measured. f LR73 cells were incubated with apoptotic thymocytes for the indicated durations and the levels of Crbn were measured. g LR73 cells transfected with the indicated plasmids were incubated with apoptotic thymocytes for the indicated durations, and association between Crbn and Orai1 was measured. h LR73 cells stimulated with TAMRA-stained apoptotic cells were stained with an anti-Crbn antibody and observed using confocal microscopy (left) and Crbn localization was quantified (right). Scale bar, 20 µm. n = 8 cells for control and n = 25 cells for adding apoptotic cells, mean ± SEM. NS not significant (two-tailed unpaired Student t test). i LR73 cells incubated with apoptotic cells were lysed and fractionized. Proteins in the fractions were detected with the indicated antibodies. j LR73 cells transfected with the indicated plasmids were incubated with apoptotic cells, and association between Orai1 and Stim1 was measured. k 293T cells were transfected with the indicated plasmids, and association between Crbn and Oria1 was measured in the presence or absence of Stim1. Data are representative of three c – g , I , k or five j independent experiments. .

    Article Snippet: Anti-Flag (Sigma, F1804), anti-HA (Santa Cruz, sc-7392), anti-GST (Santa Cruz, sc-138), anti-Crbn (Sigma, HPA045910), anti-Orai1 (Santa Cruz, sc-68895), anti-Orai1 (Alomone labs, ACC-062), anti-Orai2 (Abcam, ab180146), anti-Ampkα (Cell signaling, #2532), anti-phospho-AMPKα (cell signaling, #2535), anti-Stim1 (Abcam, ab108994), anti-Cul4A (Abcam, ab92554), anti-DDB1 (Bethyl Laboratories, A300-462A), anti-phospho-raptor (Cell signaling, #2083), anti-raptor (Cell signaling, #2280), anti-phospho-S6K (Cell signaling, #9206), anti-S6K (Cell signaling, #9202), anti-Lamin B (Santa Cruz, sc-374015), anti-Erk2 (Santa Cruz, sc-154), anti-CD16/32 (BioLegend, #101302), anti-CD11b (BioLegend, #1010207), and anti-β-Actin (Santa Cruz, sc-1616) were purchased.

    Techniques: Transfection, Incubation, Staining, Flow Cytometry, Two Tailed Test, Confocal Microscopy

    Crbn interacts with Orai1. a , b Phagocytes from the indicated mice a or Crbn deleted 293T cells by CRISPR/Cas9 b were lysed and proteins in the lysates were detected using immunoblotting. Data are representative of three independent experiments. c The transcript levels of Orai1 were analyzed by conventional PCR (bottom) or qRT-PCR (top) using cDNA synthesized from total mRNA extracted from the indicated BMDMs. n = 3 experiments, mean ± SEM. NS not significant (two-tailed unpaired Student t test). d LR73 cells were transfected with the indicated plasmid. At 1 day after transfection, the cells were lysed and proteins in the lysates were detected with the indicated antibodies. Data are representative of three independent experiments. e , f 293T cells transfected with the indicated plasmids e or BMDMs f were lysed and then the lysates were incubated with anti-FLAG antibody-conjugated agarose beads e or an anti-Orai1 antibody and protein A/G agarose beads f . Bead-bound proteins were detected with the indicated antibodies. Data are representative of five e or three f independent experiments. IP immunoprecipitation, TCL total cell lysates. g – i 293T cells were transfected with the indicated plasmids. At 2 days after transfection, the cells were lysed and the lysates were incubated with agarose beads conjugated with glutathione g , i or an anti-FLAG antibody h . Bead-bound proteins were detected with the indicated antibodies. Data are representative of four g or three h , i independent experiments. j Yeast cells transformed with the indicated plasmids were plated on selective or non-selective media. Cells on the non-selective media indicate the number of cells plated. BD binding domain, AD activation domain.

    Journal: Nature Communications

    Article Title: Crbn modulates calcium influx by regulating Orai1 during efferocytosis

    doi: 10.1038/s41467-020-19272-0

    Figure Lengend Snippet: Crbn interacts with Orai1. a , b Phagocytes from the indicated mice a or Crbn deleted 293T cells by CRISPR/Cas9 b were lysed and proteins in the lysates were detected using immunoblotting. Data are representative of three independent experiments. c The transcript levels of Orai1 were analyzed by conventional PCR (bottom) or qRT-PCR (top) using cDNA synthesized from total mRNA extracted from the indicated BMDMs. n = 3 experiments, mean ± SEM. NS not significant (two-tailed unpaired Student t test). d LR73 cells were transfected with the indicated plasmid. At 1 day after transfection, the cells were lysed and proteins in the lysates were detected with the indicated antibodies. Data are representative of three independent experiments. e , f 293T cells transfected with the indicated plasmids e or BMDMs f were lysed and then the lysates were incubated with anti-FLAG antibody-conjugated agarose beads e or an anti-Orai1 antibody and protein A/G agarose beads f . Bead-bound proteins were detected with the indicated antibodies. Data are representative of five e or three f independent experiments. IP immunoprecipitation, TCL total cell lysates. g – i 293T cells were transfected with the indicated plasmids. At 2 days after transfection, the cells were lysed and the lysates were incubated with agarose beads conjugated with glutathione g , i or an anti-FLAG antibody h . Bead-bound proteins were detected with the indicated antibodies. Data are representative of four g or three h , i independent experiments. j Yeast cells transformed with the indicated plasmids were plated on selective or non-selective media. Cells on the non-selective media indicate the number of cells plated. BD binding domain, AD activation domain.

    Article Snippet: Anti-Flag (Sigma, F1804), anti-HA (Santa Cruz, sc-7392), anti-GST (Santa Cruz, sc-138), anti-Crbn (Sigma, HPA045910), anti-Orai1 (Santa Cruz, sc-68895), anti-Orai1 (Alomone labs, ACC-062), anti-Orai2 (Abcam, ab180146), anti-Ampkα (Cell signaling, #2532), anti-phospho-AMPKα (cell signaling, #2535), anti-Stim1 (Abcam, ab108994), anti-Cul4A (Abcam, ab92554), anti-DDB1 (Bethyl Laboratories, A300-462A), anti-phospho-raptor (Cell signaling, #2083), anti-raptor (Cell signaling, #2280), anti-phospho-S6K (Cell signaling, #9206), anti-S6K (Cell signaling, #9202), anti-Lamin B (Santa Cruz, sc-374015), anti-Erk2 (Santa Cruz, sc-154), anti-CD16/32 (BioLegend, #101302), anti-CD11b (BioLegend, #1010207), and anti-β-Actin (Santa Cruz, sc-1616) were purchased.

    Techniques: Mouse Assay, CRISPR, Polymerase Chain Reaction, Quantitative RT-PCR, Synthesized, Two Tailed Test, Transfection, Plasmid Preparation, Incubation, Immunoprecipitation, Transformation Assay, Binding Assay, Activation Assay