orai1  (Alomone Labs)


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  • 94
    Name:
    Mouse Anti Human Orai1 extracellular Antibody
    Description:
    Mouse Anti Human Orai1 extracellular Antibody ALM 025 is a highly specific monoclonal antibody directed against an extracellular epitope of the human Orai1 channel The antibody can be used in western blot immunocytochemical and indirect flow cytometry applications and was designed to recognize Orai1 from human samples only
    Catalog Number:
    ALM-025
    Price:
    360.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Indirect Flow Cytometry, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified from cultured hybridoma medium.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcg
    Antibody Type:
    Monoclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Mouse
    Isotype:
    IgM
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    Structured Review

    Alomone Labs orai1
    Mouse Anti Human Orai1 extracellular Antibody
    Mouse Anti Human Orai1 extracellular Antibody ALM 025 is a highly specific monoclonal antibody directed against an extracellular epitope of the human Orai1 channel The antibody can be used in western blot immunocytochemical and indirect flow cytometry applications and was designed to recognize Orai1 from human samples only
    https://www.bioz.com/result/orai1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    orai1 - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Chronic Hypoxia Increases TRPC6 Expression and Basal Intracellular Ca2+ Concentration in Rat Distal Pulmonary Venous Smooth Muscle"

    Article Title: Chronic Hypoxia Increases TRPC6 Expression and Basal Intracellular Ca2+ Concentration in Rat Distal Pulmonary Venous Smooth Muscle

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0112007

    Effects of siOrai1 transfection on basal [Ca 2+ ] i and SOCE in rat PVSMCs. A, B : Western blot showing expression of Orai1 and α-actin protein in rat PVSMC treated with siNT, siOrai1 and transfection vehicle alone (control). E, F : The changes of SOCE in siOrai1, NTsiRNA transfected PVSMCs and transfection vehicle alone treated control. *P
    Figure Legend Snippet: Effects of siOrai1 transfection on basal [Ca 2+ ] i and SOCE in rat PVSMCs. A, B : Western blot showing expression of Orai1 and α-actin protein in rat PVSMC treated with siNT, siOrai1 and transfection vehicle alone (control). E, F : The changes of SOCE in siOrai1, NTsiRNA transfected PVSMCs and transfection vehicle alone treated control. *P

    Techniques Used: Transfection, Western Blot, Expressing

    Related Articles

    Expressing:

    Article Title: Pyridostigmine improves cardiac function and rhythmicity through RyR2 stabilization and inhibition of STIM1‐mediated calcium entry in heart failure, et al. Pyridostigmine improves cardiac function and rhythmicity through RyR2 stabilization and inhibition of STIM1‐mediated calcium entry in heart failure
    Article Snippet: .. For STIM1 (Sigma, #S6072) and Orai1 (Alomone Labs, #ALM‐025), expression was normalized to GAPDH levels. ..

    Incubation:

    Article Title: Hypoxia selectively upregulates cation channels and increases cytosolic [Ca2+] in pulmonary, but not coronary, arterial smooth muscle cells
    Article Snippet: .. Cells were incubated overnight at 4°C with a mouse monoclonal anti-Orai1 antibody (1:100, catalog no. ALM-025, Alomone) ( ) and rabbit polyclonal anti-STIM2 antibody (1:200; catalog no. ab59342, Abcam, Cambridge, MA) ( ). ..

    Concentration Assay:

    Article Title: Generation and characterization of fully human monoclonal antibodies against human Orai1 for autoimmune disease.
    Article Snippet: .. Calcium entry into T cells following antigen stimulation is crucial for nuclear factor of activated T cells (NFAT)-mediated T cell activation.. The movement of calcium is mediated by calcium release-activated calcium (CRAC) channels. ..

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  • About
  • News
  • Press Release
  • Team
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  • Bioz Stars
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  • 94
    Alomone Labs orai1
    Effects of siOrai1 transfection on basal [Ca 2+ ] i and SOCE in rat PVSMCs. A, B : Western blot showing expression of <t>Orai1</t> and α-actin protein in rat PVSMC treated with siNT, siOrai1 and transfection vehicle alone (control). E, F : The changes of SOCE in siOrai1, NTsiRNA transfected PVSMCs and transfection vehicle alone treated control. *P
    Orai1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orai1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    orai1 - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Effects of siOrai1 transfection on basal [Ca 2+ ] i and SOCE in rat PVSMCs. A, B : Western blot showing expression of Orai1 and α-actin protein in rat PVSMC treated with siNT, siOrai1 and transfection vehicle alone (control). E, F : The changes of SOCE in siOrai1, NTsiRNA transfected PVSMCs and transfection vehicle alone treated control. *P

    Journal: PLoS ONE

    Article Title: Chronic Hypoxia Increases TRPC6 Expression and Basal Intracellular Ca2+ Concentration in Rat Distal Pulmonary Venous Smooth Muscle

    doi: 10.1371/journal.pone.0112007

    Figure Lengend Snippet: Effects of siOrai1 transfection on basal [Ca 2+ ] i and SOCE in rat PVSMCs. A, B : Western blot showing expression of Orai1 and α-actin protein in rat PVSMC treated with siNT, siOrai1 and transfection vehicle alone (control). E, F : The changes of SOCE in siOrai1, NTsiRNA transfected PVSMCs and transfection vehicle alone treated control. *P

    Article Snippet: These separated proteins were then transferred to polyvinylidene difluoride membranes (pore size 0.45 µm, BioRad) and incubated with rabbit polyclonal antibodies against TRPC1 (Sigma), TRPC6 (Sigma), STIM1 (Cell Signaling) or Orai1 (Alomone labs) for overnight or mouse monoclonal antibody that was specific for α-actin (Sigma) for 1 hour.

    Techniques: Transfection, Western Blot, Expressing