collagenase 3  (Worthington Biochemical)


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  • 99
    Name:
    Collagenase Type 3
    Description:
    Lower in secondary proteolytic contaminant activities but with typical collagenase activity Suggested for mammary primary cell isolation A dialyzed lyophilized powder
    Catalog Number:
    ls004180
    Price:
    35
    Size:
    100 mg
    Source:
    Clostridium histolyticum
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical collagenase 3
    Lower in secondary proteolytic contaminant activities but with typical collagenase activity Suggested for mammary primary cell isolation A dialyzed lyophilized powder
    https://www.bioz.com/result/collagenase 3/product/Worthington Biochemical
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    collagenase 3 - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Targeting VEGF-A in myeloid cells enhances natural killer cell responses to chemotherapy and ameliorates cachexia
    Article Snippet: .. Flow cytometry analysis Tumours were digested with 0.1% collagenase type III (Worthington) and 1 U ml−1 DNAse I (Promega) and incubated at 37 °C for 1 h. One hundred and six tumour cells were incubated with Fc-Block (BD Biosciences, #553141 at 1/100) before labelling with fluorochrome-conjugated antibodies. .. The following fluorochrome-conjugated antibodies were used: anti-NK1.1 (BD Biosciences, #557391 at 1/50); anti-CD335 (NKp46, eBioscience, #25–3351 at 1/50), anti-CD45 (eBioscience, #45–0451 at 1/50), anti-CD4 (Biolegend, #100408, at 1/50), anti-CD8 (Biolegend, #100723 at 1/50) and anti-F4/80 (Biolegend, #123118 at 1/50).

    Isolation:

    Article Title: Ly6C- Monocytes Regulate Parasite-Induced Liver Inflammation by Inducing the Differentiation of Pathogenic Ly6C+ Monocytes into Macrophages
    Article Snippet: .. Isolation of liver non-parenchymal immune cells and adoptive transfer of monocytes Animals were euthanized (CO2 ) and livers were perfused through the portal vein with 10 ml of 120 U/ml collagenase type III (Worthington Biochemical Corporation) in HBSS (Gibco). .. Then, liver was disrupted mechanically and incubated in 10 ml of a 100 U/ml collagenase III solution in HBSS (25 min, 37°C).

    Article Title: Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis
    Article Snippet: .. The intestinal pieces were washed with RPMI and 10% FCS, and LPLs were isolated by digestion with 1 μg/mL DNase (Sigma-Aldrich) and 200 μg/ml Collagenase III (Worthington) for 40 min at 37 °C. .. Lamina propria lymphocytes were purified by 40–80% Percoll (GE Healthcare) gradient.

    Cytometry:

    Article Title: Targeting VEGF-A in myeloid cells enhances natural killer cell responses to chemotherapy and ameliorates cachexia
    Article Snippet: .. Flow cytometry analysis Tumours were digested with 0.1% collagenase type III (Worthington) and 1 U ml−1 DNAse I (Promega) and incubated at 37 °C for 1 h. One hundred and six tumour cells were incubated with Fc-Block (BD Biosciences, #553141 at 1/100) before labelling with fluorochrome-conjugated antibodies. .. The following fluorochrome-conjugated antibodies were used: anti-NK1.1 (BD Biosciences, #557391 at 1/50); anti-CD335 (NKp46, eBioscience, #25–3351 at 1/50), anti-CD45 (eBioscience, #45–0451 at 1/50), anti-CD4 (Biolegend, #100408, at 1/50), anti-CD8 (Biolegend, #100723 at 1/50) and anti-F4/80 (Biolegend, #123118 at 1/50).

    Incubation:

    Article Title: Targeting VEGF-A in myeloid cells enhances natural killer cell responses to chemotherapy and ameliorates cachexia
    Article Snippet: .. Flow cytometry analysis Tumours were digested with 0.1% collagenase type III (Worthington) and 1 U ml−1 DNAse I (Promega) and incubated at 37 °C for 1 h. One hundred and six tumour cells were incubated with Fc-Block (BD Biosciences, #553141 at 1/100) before labelling with fluorochrome-conjugated antibodies. .. The following fluorochrome-conjugated antibodies were used: anti-NK1.1 (BD Biosciences, #557391 at 1/50); anti-CD335 (NKp46, eBioscience, #25–3351 at 1/50), anti-CD45 (eBioscience, #45–0451 at 1/50), anti-CD4 (Biolegend, #100408, at 1/50), anti-CD8 (Biolegend, #100723 at 1/50) and anti-F4/80 (Biolegend, #123118 at 1/50).

    Adoptive Transfer Assay:

    Article Title: Ly6C- Monocytes Regulate Parasite-Induced Liver Inflammation by Inducing the Differentiation of Pathogenic Ly6C+ Monocytes into Macrophages
    Article Snippet: .. Isolation of liver non-parenchymal immune cells and adoptive transfer of monocytes Animals were euthanized (CO2 ) and livers were perfused through the portal vein with 10 ml of 120 U/ml collagenase type III (Worthington Biochemical Corporation) in HBSS (Gibco). .. Then, liver was disrupted mechanically and incubated in 10 ml of a 100 U/ml collagenase III solution in HBSS (25 min, 37°C).

    Protease Inhibitor:

    Article Title: A rigorous method to enrich for exosomes from brain tissue
    Article Snippet: .. Reagents and chemicals Hibernate®-E Medium and Dulbecco’s phosphate-buffered saline (PBS) were from ThermoFisher, PhosSTOP (PS) & Complete Protease Inhibitor (including EDTA) (PI) and D2 O were from SigmaAldrich, collagenase type 3 (Worthington #CLS-3) and RNase A from Qiagen. .. Chemicals for proteomics were from SigmaAldrich.