Ferroportin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Average 94 stars, based on 3 article reviews
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1) Product Images from "Endothelial cells are critical regulators of iron transport in a model of the human blood–brain barrier"
Article Title: Endothelial cells are critical regulators of iron transport in a model of the human blood–brain barrier
Journal: Journal of Cerebral Blood Flow & Metabolism
Figure Legend Snippet: Iron release from human endothelial cells. Human endothelial cells were loaded with 10 µCi 59 Fe-NTA overnight. (a) Release of protein-bound 59 Fe significantly increased after incubation with 1 mg/mL apo-Tf and significantly decreased after incubation with 1 mg/mL holo-Tf. (b) Release of free 59 Fe by huECs was significantly increased after incubation with apo-Tf and significantly decreased after incubation with holo-Tf. (c) 500 nM hepcidin treatment for 24 h significantly decreases free 59 Fe release. Release of protein-bound 59 Fe is unaffected by hepcidin addition. (d) Representative Western blot demonstrating incubation of huECs with 500 nM hepcidin for 24 h results in a 64% decrease in ferroportin expression. Means of three biological replicates ± SD from the treatment groups were evaluated against the “Protein-bound” or “Free Iron” conditions using two-way ANOVA with Bonferroni's posttest for significance. * p
Techniques Used: Incubation, Western Blot, Expressing