ferroportin  (Alomone Labs)


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    Alomone Labs ferroportin
    Iron release from human endothelial cells. Human endothelial cells were loaded with 10 µCi 59 Fe-NTA overnight. (a) Release of protein-bound 59 Fe significantly increased after incubation with 1 mg/mL apo-Tf and significantly decreased after incubation with 1 mg/mL holo-Tf. (b) Release of free 59 Fe by huECs was significantly increased after incubation with apo-Tf and significantly decreased after incubation with holo-Tf. (c) 500 nM hepcidin treatment for 24 h significantly decreases free 59 Fe release. Release of protein-bound 59 Fe is unaffected by hepcidin addition. (d) Representative Western blot demonstrating incubation of huECs with 500 nM hepcidin for 24 h results in a 64% decrease in <t>ferroportin</t> expression. Means of three biological replicates ± SD from the treatment groups were evaluated against the “Protein-bound” or “Free Iron” conditions using two-way ANOVA with Bonferroni's posttest for significance. * p
    Ferroportin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ferroportin/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ferroportin - by Bioz Stars, 2022-05
    90/100 stars

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    1) Product Images from "Endothelial cells are critical regulators of iron transport in a model of the human blood–brain barrier"

    Article Title: Endothelial cells are critical regulators of iron transport in a model of the human blood–brain barrier

    Journal: Journal of Cerebral Blood Flow & Metabolism

    doi: 10.1177/0271678X18783372

    Iron release from human endothelial cells. Human endothelial cells were loaded with 10 µCi 59 Fe-NTA overnight. (a) Release of protein-bound 59 Fe significantly increased after incubation with 1 mg/mL apo-Tf and significantly decreased after incubation with 1 mg/mL holo-Tf. (b) Release of free 59 Fe by huECs was significantly increased after incubation with apo-Tf and significantly decreased after incubation with holo-Tf. (c) 500 nM hepcidin treatment for 24 h significantly decreases free 59 Fe release. Release of protein-bound 59 Fe is unaffected by hepcidin addition. (d) Representative Western blot demonstrating incubation of huECs with 500 nM hepcidin for 24 h results in a 64% decrease in ferroportin expression. Means of three biological replicates ± SD from the treatment groups were evaluated against the “Protein-bound” or “Free Iron” conditions using two-way ANOVA with Bonferroni's posttest for significance. * p
    Figure Legend Snippet: Iron release from human endothelial cells. Human endothelial cells were loaded with 10 µCi 59 Fe-NTA overnight. (a) Release of protein-bound 59 Fe significantly increased after incubation with 1 mg/mL apo-Tf and significantly decreased after incubation with 1 mg/mL holo-Tf. (b) Release of free 59 Fe by huECs was significantly increased after incubation with apo-Tf and significantly decreased after incubation with holo-Tf. (c) 500 nM hepcidin treatment for 24 h significantly decreases free 59 Fe release. Release of protein-bound 59 Fe is unaffected by hepcidin addition. (d) Representative Western blot demonstrating incubation of huECs with 500 nM hepcidin for 24 h results in a 64% decrease in ferroportin expression. Means of three biological replicates ± SD from the treatment groups were evaluated against the “Protein-bound” or “Free Iron” conditions using two-way ANOVA with Bonferroni's posttest for significance. * p

    Techniques Used: Incubation, Western Blot, Expressing

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    Alomone Labs ferroportin
    Iron release from human endothelial cells. Human endothelial cells were loaded with 10 µCi 59 Fe-NTA overnight. (a) Release of protein-bound 59 Fe significantly increased after incubation with 1 mg/mL apo-Tf and significantly decreased after incubation with 1 mg/mL holo-Tf. (b) Release of free 59 Fe by huECs was significantly increased after incubation with apo-Tf and significantly decreased after incubation with holo-Tf. (c) 500 nM hepcidin treatment for 24 h significantly decreases free 59 Fe release. Release of protein-bound 59 Fe is unaffected by hepcidin addition. (d) Representative Western blot demonstrating incubation of huECs with 500 nM hepcidin for 24 h results in a 64% decrease in <t>ferroportin</t> expression. Means of three biological replicates ± SD from the treatment groups were evaluated against the “Protein-bound” or “Free Iron” conditions using two-way ANOVA with Bonferroni's posttest for significance. * p
    Ferroportin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ferroportin/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ferroportin - by Bioz Stars, 2022-05
    90/100 stars
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    Iron release from human endothelial cells. Human endothelial cells were loaded with 10 µCi 59 Fe-NTA overnight. (a) Release of protein-bound 59 Fe significantly increased after incubation with 1 mg/mL apo-Tf and significantly decreased after incubation with 1 mg/mL holo-Tf. (b) Release of free 59 Fe by huECs was significantly increased after incubation with apo-Tf and significantly decreased after incubation with holo-Tf. (c) 500 nM hepcidin treatment for 24 h significantly decreases free 59 Fe release. Release of protein-bound 59 Fe is unaffected by hepcidin addition. (d) Representative Western blot demonstrating incubation of huECs with 500 nM hepcidin for 24 h results in a 64% decrease in ferroportin expression. Means of three biological replicates ± SD from the treatment groups were evaluated against the “Protein-bound” or “Free Iron” conditions using two-way ANOVA with Bonferroni's posttest for significance. * p

    Journal: Journal of Cerebral Blood Flow & Metabolism

    Article Title: Endothelial cells are critical regulators of iron transport in a model of the human blood–brain barrier

    doi: 10.1177/0271678X18783372

    Figure Lengend Snippet: Iron release from human endothelial cells. Human endothelial cells were loaded with 10 µCi 59 Fe-NTA overnight. (a) Release of protein-bound 59 Fe significantly increased after incubation with 1 mg/mL apo-Tf and significantly decreased after incubation with 1 mg/mL holo-Tf. (b) Release of free 59 Fe by huECs was significantly increased after incubation with apo-Tf and significantly decreased after incubation with holo-Tf. (c) 500 nM hepcidin treatment for 24 h significantly decreases free 59 Fe release. Release of protein-bound 59 Fe is unaffected by hepcidin addition. (d) Representative Western blot demonstrating incubation of huECs with 500 nM hepcidin for 24 h results in a 64% decrease in ferroportin expression. Means of three biological replicates ± SD from the treatment groups were evaluated against the “Protein-bound” or “Free Iron” conditions using two-way ANOVA with Bonferroni's posttest for significance. * p

    Article Snippet: Protein was transferred onto a nitrocellulose membrane and probed for ferroportin (Alomone Labs, 1:500, AIT-001), Hft (Cell Signaling, 1:500, 4393S), Tf (Abcam, 1:5000, ab82411) or beta-actin (Sigma, 1:3000, A5441).

    Techniques: Incubation, Western Blot, Expressing