rabbit anti gephyrin polyclonal antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti gephyrin polyclonal antibody
    a Western blot and quantitative analysis of b <t>gephyrin,</t> c GlyRα3 expressions in L5 spinal cord dorsal horn at 1 h and 5 h after PGE2 intrathecal injection. The GlyRα3 protein expressions were significantly decreased in the PGE2 group at 1 h and lasted up to 5 h. The gephyrin expression decreased significantly at 5 h after PGE2 injection. d Confocal microscopic imaging showed expression of gephyrin throughout the gray matter of spinal cord. e Expression of GlyRα3 was mainly in the superficial layer of dorsal horn and in the ventral horn. Each group had n = 3–6 rats. Scale Bars: 200 μm; ** p
    Rabbit Anti Gephyrin Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gephyrin polyclonal antibody/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gephyrin polyclonal antibody - by Bioz Stars, 2022-07
    90/100 stars

    Images

    1) Product Images from "Glycine receptors expression in rat spinal cord and dorsal root ganglion in prostaglandin E2 intrathecal injection models"

    Article Title: Glycine receptors expression in rat spinal cord and dorsal root ganglion in prostaglandin E2 intrathecal injection models

    Journal: BMC Neuroscience

    doi: 10.1186/s12868-018-0470-8

    a Western blot and quantitative analysis of b gephyrin, c GlyRα3 expressions in L5 spinal cord dorsal horn at 1 h and 5 h after PGE2 intrathecal injection. The GlyRα3 protein expressions were significantly decreased in the PGE2 group at 1 h and lasted up to 5 h. The gephyrin expression decreased significantly at 5 h after PGE2 injection. d Confocal microscopic imaging showed expression of gephyrin throughout the gray matter of spinal cord. e Expression of GlyRα3 was mainly in the superficial layer of dorsal horn and in the ventral horn. Each group had n = 3–6 rats. Scale Bars: 200 μm; ** p
    Figure Legend Snippet: a Western blot and quantitative analysis of b gephyrin, c GlyRα3 expressions in L5 spinal cord dorsal horn at 1 h and 5 h after PGE2 intrathecal injection. The GlyRα3 protein expressions were significantly decreased in the PGE2 group at 1 h and lasted up to 5 h. The gephyrin expression decreased significantly at 5 h after PGE2 injection. d Confocal microscopic imaging showed expression of gephyrin throughout the gray matter of spinal cord. e Expression of GlyRα3 was mainly in the superficial layer of dorsal horn and in the ventral horn. Each group had n = 3–6 rats. Scale Bars: 200 μm; ** p

    Techniques Used: Western Blot, Injection, Expressing, Imaging

    a Western blot and quantitative analysis of b gephyrin, c GlyRα1, and d GlyRα3 protein expressions in L5 DRG at 1 h and 5 h after PGE2 intrathecal injection. The gephyrin, GlyRα1 and GlyRα3 protein expressions were significantly decreased in the PGE2 group at 5 h. Confocal microscopic imaging showed expression of e gephyrin, f GlyRα1 and g GlyRα3 localized mainly in the neuron cells. Each group had n = 3–6 rats. Scale Bars: 20 μm; ** p
    Figure Legend Snippet: a Western blot and quantitative analysis of b gephyrin, c GlyRα1, and d GlyRα3 protein expressions in L5 DRG at 1 h and 5 h after PGE2 intrathecal injection. The gephyrin, GlyRα1 and GlyRα3 protein expressions were significantly decreased in the PGE2 group at 5 h. Confocal microscopic imaging showed expression of e gephyrin, f GlyRα1 and g GlyRα3 localized mainly in the neuron cells. Each group had n = 3–6 rats. Scale Bars: 20 μm; ** p

    Techniques Used: Western Blot, Injection, Imaging, Expressing

    Triple immunofluorescence staining showing GlyRα3, Gephyrin and NeuN co-localization in the L5 spinal cord. Positive for GlyRα3 are shown in green ( a ), positive for Gephyrin are shown in red ( b ) and positive for NeuN are shown in blue ( c ). Double-labelled images of GlyRα3 and Gephyrin ( d ), GlyRα3 and NeuN ( e ) are indicated. f The merged image demonstrates co-localization of GlyRα3, Gephyrin in Neurons. Scale bars = 200 µm, 20 µm
    Figure Legend Snippet: Triple immunofluorescence staining showing GlyRα3, Gephyrin and NeuN co-localization in the L5 spinal cord. Positive for GlyRα3 are shown in green ( a ), positive for Gephyrin are shown in red ( b ) and positive for NeuN are shown in blue ( c ). Double-labelled images of GlyRα3 and Gephyrin ( d ), GlyRα3 and NeuN ( e ) are indicated. f The merged image demonstrates co-localization of GlyRα3, Gephyrin in Neurons. Scale bars = 200 µm, 20 µm

    Techniques Used: Immunofluorescence, Staining

    Triple immunofluorescence staining showing GlyRα3, Gephyrin and NeuN co-localization in the L5 DRG. Positive for GlyRα3 are shown in green ( a ), positive for Gephyrin are shown in red ( b ) and positive for NeuN are shown in blue ( c ). Double-labelled images of GlyRα3 and Gephyrin ( d ), GlyRα3 and NeuN ( e ) are indicated. f The merged image demonstrates co-localization of GlyRα3, Gephyrin in Neurons of DRG. IF experiments from three different sham controls were performed. Secondary Abs only were included as negative controls. Scale bars = 20 µm
    Figure Legend Snippet: Triple immunofluorescence staining showing GlyRα3, Gephyrin and NeuN co-localization in the L5 DRG. Positive for GlyRα3 are shown in green ( a ), positive for Gephyrin are shown in red ( b ) and positive for NeuN are shown in blue ( c ). Double-labelled images of GlyRα3 and Gephyrin ( d ), GlyRα3 and NeuN ( e ) are indicated. f The merged image demonstrates co-localization of GlyRα3, Gephyrin in Neurons of DRG. IF experiments from three different sham controls were performed. Secondary Abs only were included as negative controls. Scale bars = 20 µm

    Techniques Used: Immunofluorescence, Staining

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    Alomone Labs rabbit anti gephyrin polyclonal antibody
    a Western blot and quantitative analysis of b <t>gephyrin,</t> c GlyRα3 expressions in L5 spinal cord dorsal horn at 1 h and 5 h after PGE2 intrathecal injection. The GlyRα3 protein expressions were significantly decreased in the PGE2 group at 1 h and lasted up to 5 h. The gephyrin expression decreased significantly at 5 h after PGE2 injection. d Confocal microscopic imaging showed expression of gephyrin throughout the gray matter of spinal cord. e Expression of GlyRα3 was mainly in the superficial layer of dorsal horn and in the ventral horn. Each group had n = 3–6 rats. Scale Bars: 200 μm; ** p
    Rabbit Anti Gephyrin Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gephyrin polyclonal antibody/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gephyrin polyclonal antibody - by Bioz Stars, 2022-07
    90/100 stars
      Buy from Supplier

    Image Search Results


    a Western blot and quantitative analysis of b gephyrin, c GlyRα3 expressions in L5 spinal cord dorsal horn at 1 h and 5 h after PGE2 intrathecal injection. The GlyRα3 protein expressions were significantly decreased in the PGE2 group at 1 h and lasted up to 5 h. The gephyrin expression decreased significantly at 5 h after PGE2 injection. d Confocal microscopic imaging showed expression of gephyrin throughout the gray matter of spinal cord. e Expression of GlyRα3 was mainly in the superficial layer of dorsal horn and in the ventral horn. Each group had n = 3–6 rats. Scale Bars: 200 μm; ** p

    Journal: BMC Neuroscience

    Article Title: Glycine receptors expression in rat spinal cord and dorsal root ganglion in prostaglandin E2 intrathecal injection models

    doi: 10.1186/s12868-018-0470-8

    Figure Lengend Snippet: a Western blot and quantitative analysis of b gephyrin, c GlyRα3 expressions in L5 spinal cord dorsal horn at 1 h and 5 h after PGE2 intrathecal injection. The GlyRα3 protein expressions were significantly decreased in the PGE2 group at 1 h and lasted up to 5 h. The gephyrin expression decreased significantly at 5 h after PGE2 injection. d Confocal microscopic imaging showed expression of gephyrin throughout the gray matter of spinal cord. e Expression of GlyRα3 was mainly in the superficial layer of dorsal horn and in the ventral horn. Each group had n = 3–6 rats. Scale Bars: 200 μm; ** p

    Article Snippet: The membranes were firstly blocked with 5% milk in phosphate-buffered saline (PBS) with 0.1% Tween-20 for 1 h at room temperature, and then probed overnight at 4 °C with Rabbit anti-GlyRα1 Polyclonal Antibody (1:1000, AGR-001, Alomone Labs, Israel), Rabbit anti-gephyrin Polyclonal Antibody (1:2000, AIP-005, Alomone Labs, Israel), goat Anti-GlyRα3 polyclonal Antibody (1:500, SC-17282, Santa Cruz Biotechnology, Santa Cruz, CA, USA), or mouse anti-actin monoclonal antibody (1:10,000, MAB1501, Indianapolis, IN, USA) primary antibody to detect the expression of GlyRα1, GlyRα3 and gephyrin in the DRG and spinal cord.

    Techniques: Western Blot, Injection, Expressing, Imaging

    a Western blot and quantitative analysis of b gephyrin, c GlyRα1, and d GlyRα3 protein expressions in L5 DRG at 1 h and 5 h after PGE2 intrathecal injection. The gephyrin, GlyRα1 and GlyRα3 protein expressions were significantly decreased in the PGE2 group at 5 h. Confocal microscopic imaging showed expression of e gephyrin, f GlyRα1 and g GlyRα3 localized mainly in the neuron cells. Each group had n = 3–6 rats. Scale Bars: 20 μm; ** p

    Journal: BMC Neuroscience

    Article Title: Glycine receptors expression in rat spinal cord and dorsal root ganglion in prostaglandin E2 intrathecal injection models

    doi: 10.1186/s12868-018-0470-8

    Figure Lengend Snippet: a Western blot and quantitative analysis of b gephyrin, c GlyRα1, and d GlyRα3 protein expressions in L5 DRG at 1 h and 5 h after PGE2 intrathecal injection. The gephyrin, GlyRα1 and GlyRα3 protein expressions were significantly decreased in the PGE2 group at 5 h. Confocal microscopic imaging showed expression of e gephyrin, f GlyRα1 and g GlyRα3 localized mainly in the neuron cells. Each group had n = 3–6 rats. Scale Bars: 20 μm; ** p

    Article Snippet: The membranes were firstly blocked with 5% milk in phosphate-buffered saline (PBS) with 0.1% Tween-20 for 1 h at room temperature, and then probed overnight at 4 °C with Rabbit anti-GlyRα1 Polyclonal Antibody (1:1000, AGR-001, Alomone Labs, Israel), Rabbit anti-gephyrin Polyclonal Antibody (1:2000, AIP-005, Alomone Labs, Israel), goat Anti-GlyRα3 polyclonal Antibody (1:500, SC-17282, Santa Cruz Biotechnology, Santa Cruz, CA, USA), or mouse anti-actin monoclonal antibody (1:10,000, MAB1501, Indianapolis, IN, USA) primary antibody to detect the expression of GlyRα1, GlyRα3 and gephyrin in the DRG and spinal cord.

    Techniques: Western Blot, Injection, Imaging, Expressing

    Triple immunofluorescence staining showing GlyRα3, Gephyrin and NeuN co-localization in the L5 spinal cord. Positive for GlyRα3 are shown in green ( a ), positive for Gephyrin are shown in red ( b ) and positive for NeuN are shown in blue ( c ). Double-labelled images of GlyRα3 and Gephyrin ( d ), GlyRα3 and NeuN ( e ) are indicated. f The merged image demonstrates co-localization of GlyRα3, Gephyrin in Neurons. Scale bars = 200 µm, 20 µm

    Journal: BMC Neuroscience

    Article Title: Glycine receptors expression in rat spinal cord and dorsal root ganglion in prostaglandin E2 intrathecal injection models

    doi: 10.1186/s12868-018-0470-8

    Figure Lengend Snippet: Triple immunofluorescence staining showing GlyRα3, Gephyrin and NeuN co-localization in the L5 spinal cord. Positive for GlyRα3 are shown in green ( a ), positive for Gephyrin are shown in red ( b ) and positive for NeuN are shown in blue ( c ). Double-labelled images of GlyRα3 and Gephyrin ( d ), GlyRα3 and NeuN ( e ) are indicated. f The merged image demonstrates co-localization of GlyRα3, Gephyrin in Neurons. Scale bars = 200 µm, 20 µm

    Article Snippet: The membranes were firstly blocked with 5% milk in phosphate-buffered saline (PBS) with 0.1% Tween-20 for 1 h at room temperature, and then probed overnight at 4 °C with Rabbit anti-GlyRα1 Polyclonal Antibody (1:1000, AGR-001, Alomone Labs, Israel), Rabbit anti-gephyrin Polyclonal Antibody (1:2000, AIP-005, Alomone Labs, Israel), goat Anti-GlyRα3 polyclonal Antibody (1:500, SC-17282, Santa Cruz Biotechnology, Santa Cruz, CA, USA), or mouse anti-actin monoclonal antibody (1:10,000, MAB1501, Indianapolis, IN, USA) primary antibody to detect the expression of GlyRα1, GlyRα3 and gephyrin in the DRG and spinal cord.

    Techniques: Immunofluorescence, Staining

    Triple immunofluorescence staining showing GlyRα3, Gephyrin and NeuN co-localization in the L5 DRG. Positive for GlyRα3 are shown in green ( a ), positive for Gephyrin are shown in red ( b ) and positive for NeuN are shown in blue ( c ). Double-labelled images of GlyRα3 and Gephyrin ( d ), GlyRα3 and NeuN ( e ) are indicated. f The merged image demonstrates co-localization of GlyRα3, Gephyrin in Neurons of DRG. IF experiments from three different sham controls were performed. Secondary Abs only were included as negative controls. Scale bars = 20 µm

    Journal: BMC Neuroscience

    Article Title: Glycine receptors expression in rat spinal cord and dorsal root ganglion in prostaglandin E2 intrathecal injection models

    doi: 10.1186/s12868-018-0470-8

    Figure Lengend Snippet: Triple immunofluorescence staining showing GlyRα3, Gephyrin and NeuN co-localization in the L5 DRG. Positive for GlyRα3 are shown in green ( a ), positive for Gephyrin are shown in red ( b ) and positive for NeuN are shown in blue ( c ). Double-labelled images of GlyRα3 and Gephyrin ( d ), GlyRα3 and NeuN ( e ) are indicated. f The merged image demonstrates co-localization of GlyRα3, Gephyrin in Neurons of DRG. IF experiments from three different sham controls were performed. Secondary Abs only were included as negative controls. Scale bars = 20 µm

    Article Snippet: The membranes were firstly blocked with 5% milk in phosphate-buffered saline (PBS) with 0.1% Tween-20 for 1 h at room temperature, and then probed overnight at 4 °C with Rabbit anti-GlyRα1 Polyclonal Antibody (1:1000, AGR-001, Alomone Labs, Israel), Rabbit anti-gephyrin Polyclonal Antibody (1:2000, AIP-005, Alomone Labs, Israel), goat Anti-GlyRα3 polyclonal Antibody (1:500, SC-17282, Santa Cruz Biotechnology, Santa Cruz, CA, USA), or mouse anti-actin monoclonal antibody (1:10,000, MAB1501, Indianapolis, IN, USA) primary antibody to detect the expression of GlyRα1, GlyRα3 and gephyrin in the DRG and spinal cord.

    Techniques: Immunofluorescence, Staining