rabbit anti glut4 antibody  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Name:
    Anti GLUT4 Antibody
    Description:
    Anti GLUT4 Antibody AGT 024 is a highly specific antibody directed against an epitope of the human Glucose Transporter 4 protein The antibody can be used in western blot and immunohistochemistry applications It has been designed to recognize GLUT4 from human rat and mouse samples
    Catalog Number:
    AGT-024
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunofluorescence, Immunohistochemistry, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    Buy from Supplier


    Structured Review

    Alomone Labs rabbit anti glut4 antibody
    Anti GLUT4 Antibody
    Anti GLUT4 Antibody AGT 024 is a highly specific antibody directed against an epitope of the human Glucose Transporter 4 protein The antibody can be used in western blot and immunohistochemistry applications It has been designed to recognize GLUT4 from human rat and mouse samples
    https://www.bioz.com/result/rabbit anti glut4 antibody/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti glut4 antibody - by Bioz Stars, 2021-09
    90/100 stars

    Images

    1) Product Images from "Glut4 mobilization supports energetic demands of active synapses"

    Article Title: Glut4 mobilization supports energetic demands of active synapses

    Journal: Neuron

    doi: 10.1016/j.neuron.2016.12.020

    Neuronal firing drives Glut4 vesicles to the presynaptic plasma membrane (A-D) Neurons expressing Glut4-pH were electrically stimulated with 600 AP. (A) Glut4-pH (pseudocolor) and the synaptic vesicle marker vGlut-mO (red) before and after stimulation. Neutralization of Glut4-pH vesicles with NH 4 Cl (white) reveals total axonal pool. (B) Average trace of Glut4-pH (n = 12 cells) with 600 AP-stimulation with the inset showing response after the first 100 AP. ΔF values were normalized to maximal ΔF from NH 4 Cl treatment. Error bars are shown in gray and are SEM. (C) a sample trace where stimulation was followed by quenching of extracellular pHluorin with MES acid and neutralization of Glut4-pH vesicles with NH 4 .
    Figure Legend Snippet: Neuronal firing drives Glut4 vesicles to the presynaptic plasma membrane (A-D) Neurons expressing Glut4-pH were electrically stimulated with 600 AP. (A) Glut4-pH (pseudocolor) and the synaptic vesicle marker vGlut-mO (red) before and after stimulation. Neutralization of Glut4-pH vesicles with NH 4 Cl (white) reveals total axonal pool. (B) Average trace of Glut4-pH (n = 12 cells) with 600 AP-stimulation with the inset showing response after the first 100 AP. ΔF values were normalized to maximal ΔF from NH 4 Cl treatment. Error bars are shown in gray and are SEM. (C) a sample trace where stimulation was followed by quenching of extracellular pHluorin with MES acid and neutralization of Glut4-pH vesicles with NH 4 .

    Techniques Used: Expressing, Marker, Neutralization

    Glut4 vesicles are distinct from synaptic vesicles (A) The pH of axonal Glut4 vesicle measured from responses to acid quenching and neutralization with NH 4 Cl. The box and whisker plot represents median (line), 25 th -75 th percentile (box), and min-max (whisker). Mean pH: 6.1 ± 0.1; n = 9 cells. (B) Sample traces from boutons co-expressing Glut4-pH and vGlut-mO stimulated with 600 AP (20 Hz). (C) Decay half-time (sec) of Glut4-pH and vGlut-pH (in separate cells) after stimulation with 600 AP (10 Hz): Glut4-pH: 101 ± 17, vGlutpH: 6 ± 1 ; n = 7-10 cells per condition. *** P
    Figure Legend Snippet: Glut4 vesicles are distinct from synaptic vesicles (A) The pH of axonal Glut4 vesicle measured from responses to acid quenching and neutralization with NH 4 Cl. The box and whisker plot represents median (line), 25 th -75 th percentile (box), and min-max (whisker). Mean pH: 6.1 ± 0.1; n = 9 cells. (B) Sample traces from boutons co-expressing Glut4-pH and vGlut-mO stimulated with 600 AP (20 Hz). (C) Decay half-time (sec) of Glut4-pH and vGlut-pH (in separate cells) after stimulation with 600 AP (10 Hz): Glut4-pH: 101 ± 17, vGlutpH: 6 ± 1 ; n = 7-10 cells per condition. *** P

    Techniques Used: Neutralization, Whisker Assay, Expressing, Size-exclusion Chromatography

    Glut4 is required for synaptic vesicle recycling following bursts of AP firing (A-D) Sample vGlut-pH traces in response to 100 AP (10 Hz) in (A) control, Glut4 KD, and Glut4 KD neurons expressing shRNA-resistant Glut4-RFP, or (B) in Glut4 KD neurons where stimulation was followed by quenching of extracellular pHluorin with MES acid. (C) Average endocytic block measured as the fraction of vGlut-pH signal remaining at two endocytic time constants (2⊺) of the control at the end of stimulation. Control: 0.15 ± 0.03, Glut4 KD: 0.7 ± 0.1, rescue: 0.26 ± 0.07; n = 13-17 cells. (D) Average exocytosis of vGlut-pH measured as ΔF at the end of 100 AP normalized to ΔF NH4Cl .
    Figure Legend Snippet: Glut4 is required for synaptic vesicle recycling following bursts of AP firing (A-D) Sample vGlut-pH traces in response to 100 AP (10 Hz) in (A) control, Glut4 KD, and Glut4 KD neurons expressing shRNA-resistant Glut4-RFP, or (B) in Glut4 KD neurons where stimulation was followed by quenching of extracellular pHluorin with MES acid. (C) Average endocytic block measured as the fraction of vGlut-pH signal remaining at two endocytic time constants (2⊺) of the control at the end of stimulation. Control: 0.15 ± 0.03, Glut4 KD: 0.7 ± 0.1, rescue: 0.26 ± 0.07; n = 13-17 cells. (D) Average exocytosis of vGlut-pH measured as ΔF at the end of 100 AP normalized to ΔF NH4Cl .

    Techniques Used: Expressing, shRNA, Blocking Assay

    Energetic requirement for glycolysis and glucose uptake increases with duration of activity (A) Normalized vGlut-pH traces in response to 10, 50 and 100 AP (10Hz) before and 5 min after incubation with dGlu. (B) Normalized vGlut-pH traces of Glut4 KD neurons stimulated with 10 or 100 AP (10 Hz). (C) Average endocytic block for varying AP trains measured as the fraction of the maximal fluorescence remaining after 2 endocytic time constants in treated (dGlu or Glut4 KD) compared to control conditions, n = 4-21 cells per condition. All error bars are SEM.
    Figure Legend Snippet: Energetic requirement for glycolysis and glucose uptake increases with duration of activity (A) Normalized vGlut-pH traces in response to 10, 50 and 100 AP (10Hz) before and 5 min after incubation with dGlu. (B) Normalized vGlut-pH traces of Glut4 KD neurons stimulated with 10 or 100 AP (10 Hz). (C) Average endocytic block for varying AP trains measured as the fraction of the maximal fluorescence remaining after 2 endocytic time constants in treated (dGlu or Glut4 KD) compared to control conditions, n = 4-21 cells per condition. All error bars are SEM.

    Techniques Used: Activity Assay, Incubation, Blocking Assay, Fluorescence

    The glucose transporter Glut4 is expressed in the brain and is present at nerve terminals .
    Figure Legend Snippet: The glucose transporter Glut4 is expressed in the brain and is present at nerve terminals .

    Techniques Used:

    2) Product Images from "Glut4 mobilization supports energetic demands of active synapses"

    Article Title: Glut4 mobilization supports energetic demands of active synapses

    Journal: Neuron

    doi: 10.1016/j.neuron.2016.12.020

    Neuronal firing drives Glut4 vesicles to the presynaptic plasma membrane (A-D) Neurons expressing Glut4-pH were electrically stimulated with 600 AP. (A) Glut4-pH (pseudocolor) and the synaptic vesicle marker vGlut-mO (red) before and after stimulation. Neutralization of Glut4-pH vesicles with NH 4 Cl (white) reveals total axonal pool. (B) Average trace of Glut4-pH (n = 12 cells) with 600 AP-stimulation with the inset showing response after the first 100 AP. ΔF values were normalized to maximal ΔF from NH 4 Cl treatment. Error bars are shown in gray and are SEM. (C) a sample trace where stimulation was followed by quenching of extracellular pHluorin with MES acid and neutralization of Glut4-pH vesicles with NH 4 .
    Figure Legend Snippet: Neuronal firing drives Glut4 vesicles to the presynaptic plasma membrane (A-D) Neurons expressing Glut4-pH were electrically stimulated with 600 AP. (A) Glut4-pH (pseudocolor) and the synaptic vesicle marker vGlut-mO (red) before and after stimulation. Neutralization of Glut4-pH vesicles with NH 4 Cl (white) reveals total axonal pool. (B) Average trace of Glut4-pH (n = 12 cells) with 600 AP-stimulation with the inset showing response after the first 100 AP. ΔF values were normalized to maximal ΔF from NH 4 Cl treatment. Error bars are shown in gray and are SEM. (C) a sample trace where stimulation was followed by quenching of extracellular pHluorin with MES acid and neutralization of Glut4-pH vesicles with NH 4 .

    Techniques Used: Expressing, Marker, Neutralization

    Glut4 vesicles are distinct from synaptic vesicles (A) The pH of axonal Glut4 vesicle measured from responses to acid quenching and neutralization with NH 4 Cl. The box and whisker plot represents median (line), 25 th -75 th percentile (box), and min-max (whisker). Mean pH: 6.1 ± 0.1; n = 9 cells. (B) Sample traces from boutons co-expressing Glut4-pH and vGlut-mO stimulated with 600 AP (20 Hz). (C) Decay half-time (sec) of Glut4-pH and vGlut-pH (in separate cells) after stimulation with 600 AP (10 Hz): Glut4-pH: 101 ± 17, vGlutpH: 6 ± 1 ; n = 7-10 cells per condition. *** P
    Figure Legend Snippet: Glut4 vesicles are distinct from synaptic vesicles (A) The pH of axonal Glut4 vesicle measured from responses to acid quenching and neutralization with NH 4 Cl. The box and whisker plot represents median (line), 25 th -75 th percentile (box), and min-max (whisker). Mean pH: 6.1 ± 0.1; n = 9 cells. (B) Sample traces from boutons co-expressing Glut4-pH and vGlut-mO stimulated with 600 AP (20 Hz). (C) Decay half-time (sec) of Glut4-pH and vGlut-pH (in separate cells) after stimulation with 600 AP (10 Hz): Glut4-pH: 101 ± 17, vGlutpH: 6 ± 1 ; n = 7-10 cells per condition. *** P

    Techniques Used: Neutralization, Whisker Assay, Expressing

    Glut4 is required for synaptic vesicle recycling following bursts of AP firing (A-D) Sample vGlut-pH traces in response to 100 AP (10 Hz) in (A) control, Glut4 KD, and Glut4 KD neurons expressing shRNA-resistant Glut4-RFP, or (B) in Glut4 KD neurons where stimulation was followed by quenching of extracellular pHluorin with MES acid. (C) Average endocytic block measured as the fraction of vGlut-pH signal remaining at two endocytic time constants (2⊺) of the control at the end of stimulation. Control: 0.15 ± 0.03, Glut4 KD: 0.7 ± 0.1, rescue: 0.26 ± 0.07; n = 13-17 cells. (D) Average exocytosis of vGlut-pH measured as ΔF at the end of 100 AP normalized to ΔF NH4Cl .
    Figure Legend Snippet: Glut4 is required for synaptic vesicle recycling following bursts of AP firing (A-D) Sample vGlut-pH traces in response to 100 AP (10 Hz) in (A) control, Glut4 KD, and Glut4 KD neurons expressing shRNA-resistant Glut4-RFP, or (B) in Glut4 KD neurons where stimulation was followed by quenching of extracellular pHluorin with MES acid. (C) Average endocytic block measured as the fraction of vGlut-pH signal remaining at two endocytic time constants (2⊺) of the control at the end of stimulation. Control: 0.15 ± 0.03, Glut4 KD: 0.7 ± 0.1, rescue: 0.26 ± 0.07; n = 13-17 cells. (D) Average exocytosis of vGlut-pH measured as ΔF at the end of 100 AP normalized to ΔF NH4Cl .

    Techniques Used: Expressing, shRNA, Blocking Assay

    Energetic requirement for glycolysis and glucose uptake increases with duration of activity (A) Normalized vGlut-pH traces in response to 10, 50 and 100 AP (10Hz) before and 5 min after incubation with dGlu. (B) Normalized vGlut-pH traces of Glut4 KD neurons stimulated with 10 or 100 AP (10 Hz). (C) Average endocytic block for varying AP trains measured as the fraction of the maximal fluorescence remaining after 2 endocytic time constants in treated (dGlu or Glut4 KD) compared to control conditions, n = 4-21 cells per condition. All error bars are SEM.
    Figure Legend Snippet: Energetic requirement for glycolysis and glucose uptake increases with duration of activity (A) Normalized vGlut-pH traces in response to 10, 50 and 100 AP (10Hz) before and 5 min after incubation with dGlu. (B) Normalized vGlut-pH traces of Glut4 KD neurons stimulated with 10 or 100 AP (10 Hz). (C) Average endocytic block for varying AP trains measured as the fraction of the maximal fluorescence remaining after 2 endocytic time constants in treated (dGlu or Glut4 KD) compared to control conditions, n = 4-21 cells per condition. All error bars are SEM.

    Techniques Used: Activity Assay, Incubation, Blocking Assay, Fluorescence

    The glucose transporter Glut4 is expressed in the brain and is present at nerve terminals .
    Figure Legend Snippet: The glucose transporter Glut4 is expressed in the brain and is present at nerve terminals .

    Techniques Used:

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Alomone Labs rabbit anti glut4 antibody
    Neuronal firing drives <t>Glut4</t> vesicles to the presynaptic plasma membrane (A-D) Neurons expressing Glut4-pH were electrically stimulated with 600 AP. (A) Glut4-pH (pseudocolor) and the synaptic vesicle marker vGlut-mO (red) before and after stimulation. Neutralization of Glut4-pH vesicles with NH 4 Cl (white) reveals total axonal pool. (B) Average trace of Glut4-pH (n = 12 cells) with 600 AP-stimulation with the inset showing response after the first 100 AP. ΔF values were normalized to maximal ΔF from NH 4 Cl treatment. Error bars are shown in gray and are SEM. (C) a sample trace where stimulation was followed by quenching of extracellular pHluorin with MES acid and neutralization of Glut4-pH vesicles with NH 4 .
    Rabbit Anti Glut4 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti glut4 antibody/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti glut4 antibody - by Bioz Stars, 2021-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Neuronal firing drives Glut4 vesicles to the presynaptic plasma membrane (A-D) Neurons expressing Glut4-pH were electrically stimulated with 600 AP. (A) Glut4-pH (pseudocolor) and the synaptic vesicle marker vGlut-mO (red) before and after stimulation. Neutralization of Glut4-pH vesicles with NH 4 Cl (white) reveals total axonal pool. (B) Average trace of Glut4-pH (n = 12 cells) with 600 AP-stimulation with the inset showing response after the first 100 AP. ΔF values were normalized to maximal ΔF from NH 4 Cl treatment. Error bars are shown in gray and are SEM. (C) a sample trace where stimulation was followed by quenching of extracellular pHluorin with MES acid and neutralization of Glut4-pH vesicles with NH 4 .

    Journal: Neuron

    Article Title: Glut4 mobilization supports energetic demands of active synapses

    doi: 10.1016/j.neuron.2016.12.020

    Figure Lengend Snippet: Neuronal firing drives Glut4 vesicles to the presynaptic plasma membrane (A-D) Neurons expressing Glut4-pH were electrically stimulated with 600 AP. (A) Glut4-pH (pseudocolor) and the synaptic vesicle marker vGlut-mO (red) before and after stimulation. Neutralization of Glut4-pH vesicles with NH 4 Cl (white) reveals total axonal pool. (B) Average trace of Glut4-pH (n = 12 cells) with 600 AP-stimulation with the inset showing response after the first 100 AP. ΔF values were normalized to maximal ΔF from NH 4 Cl treatment. Error bars are shown in gray and are SEM. (C) a sample trace where stimulation was followed by quenching of extracellular pHluorin with MES acid and neutralization of Glut4-pH vesicles with NH 4 .

    Article Snippet: The following primary antibodies were used: rabbit anti-Glut4 antibody (Alomone Labs AGT-024, RRID: AB_2631197), guinea pig anti-vGlut1 (Millipore AB5905, RRID: AB_2301751), mouse anti-Calbindin (SWANT C9638, RRID: AB_2314070).

    Techniques: Expressing, Marker, Neutralization

    Glut4 vesicles are distinct from synaptic vesicles (A) The pH of axonal Glut4 vesicle measured from responses to acid quenching and neutralization with NH 4 Cl. The box and whisker plot represents median (line), 25 th -75 th percentile (box), and min-max (whisker). Mean pH: 6.1 ± 0.1; n = 9 cells. (B) Sample traces from boutons co-expressing Glut4-pH and vGlut-mO stimulated with 600 AP (20 Hz). (C) Decay half-time (sec) of Glut4-pH and vGlut-pH (in separate cells) after stimulation with 600 AP (10 Hz): Glut4-pH: 101 ± 17, vGlutpH: 6 ± 1 ; n = 7-10 cells per condition. *** P

    Journal: Neuron

    Article Title: Glut4 mobilization supports energetic demands of active synapses

    doi: 10.1016/j.neuron.2016.12.020

    Figure Lengend Snippet: Glut4 vesicles are distinct from synaptic vesicles (A) The pH of axonal Glut4 vesicle measured from responses to acid quenching and neutralization with NH 4 Cl. The box and whisker plot represents median (line), 25 th -75 th percentile (box), and min-max (whisker). Mean pH: 6.1 ± 0.1; n = 9 cells. (B) Sample traces from boutons co-expressing Glut4-pH and vGlut-mO stimulated with 600 AP (20 Hz). (C) Decay half-time (sec) of Glut4-pH and vGlut-pH (in separate cells) after stimulation with 600 AP (10 Hz): Glut4-pH: 101 ± 17, vGlutpH: 6 ± 1 ; n = 7-10 cells per condition. *** P

    Article Snippet: The following primary antibodies were used: rabbit anti-Glut4 antibody (Alomone Labs AGT-024, RRID: AB_2631197), guinea pig anti-vGlut1 (Millipore AB5905, RRID: AB_2301751), mouse anti-Calbindin (SWANT C9638, RRID: AB_2314070).

    Techniques: Neutralization, Whisker Assay, Expressing, Size-exclusion Chromatography

    Glut4 is required for synaptic vesicle recycling following bursts of AP firing (A-D) Sample vGlut-pH traces in response to 100 AP (10 Hz) in (A) control, Glut4 KD, and Glut4 KD neurons expressing shRNA-resistant Glut4-RFP, or (B) in Glut4 KD neurons where stimulation was followed by quenching of extracellular pHluorin with MES acid. (C) Average endocytic block measured as the fraction of vGlut-pH signal remaining at two endocytic time constants (2⊺) of the control at the end of stimulation. Control: 0.15 ± 0.03, Glut4 KD: 0.7 ± 0.1, rescue: 0.26 ± 0.07; n = 13-17 cells. (D) Average exocytosis of vGlut-pH measured as ΔF at the end of 100 AP normalized to ΔF NH4Cl .

    Journal: Neuron

    Article Title: Glut4 mobilization supports energetic demands of active synapses

    doi: 10.1016/j.neuron.2016.12.020

    Figure Lengend Snippet: Glut4 is required for synaptic vesicle recycling following bursts of AP firing (A-D) Sample vGlut-pH traces in response to 100 AP (10 Hz) in (A) control, Glut4 KD, and Glut4 KD neurons expressing shRNA-resistant Glut4-RFP, or (B) in Glut4 KD neurons where stimulation was followed by quenching of extracellular pHluorin with MES acid. (C) Average endocytic block measured as the fraction of vGlut-pH signal remaining at two endocytic time constants (2⊺) of the control at the end of stimulation. Control: 0.15 ± 0.03, Glut4 KD: 0.7 ± 0.1, rescue: 0.26 ± 0.07; n = 13-17 cells. (D) Average exocytosis of vGlut-pH measured as ΔF at the end of 100 AP normalized to ΔF NH4Cl .

    Article Snippet: The following primary antibodies were used: rabbit anti-Glut4 antibody (Alomone Labs AGT-024, RRID: AB_2631197), guinea pig anti-vGlut1 (Millipore AB5905, RRID: AB_2301751), mouse anti-Calbindin (SWANT C9638, RRID: AB_2314070).

    Techniques: Expressing, shRNA, Blocking Assay

    Energetic requirement for glycolysis and glucose uptake increases with duration of activity (A) Normalized vGlut-pH traces in response to 10, 50 and 100 AP (10Hz) before and 5 min after incubation with dGlu. (B) Normalized vGlut-pH traces of Glut4 KD neurons stimulated with 10 or 100 AP (10 Hz). (C) Average endocytic block for varying AP trains measured as the fraction of the maximal fluorescence remaining after 2 endocytic time constants in treated (dGlu or Glut4 KD) compared to control conditions, n = 4-21 cells per condition. All error bars are SEM.

    Journal: Neuron

    Article Title: Glut4 mobilization supports energetic demands of active synapses

    doi: 10.1016/j.neuron.2016.12.020

    Figure Lengend Snippet: Energetic requirement for glycolysis and glucose uptake increases with duration of activity (A) Normalized vGlut-pH traces in response to 10, 50 and 100 AP (10Hz) before and 5 min after incubation with dGlu. (B) Normalized vGlut-pH traces of Glut4 KD neurons stimulated with 10 or 100 AP (10 Hz). (C) Average endocytic block for varying AP trains measured as the fraction of the maximal fluorescence remaining after 2 endocytic time constants in treated (dGlu or Glut4 KD) compared to control conditions, n = 4-21 cells per condition. All error bars are SEM.

    Article Snippet: The following primary antibodies were used: rabbit anti-Glut4 antibody (Alomone Labs AGT-024, RRID: AB_2631197), guinea pig anti-vGlut1 (Millipore AB5905, RRID: AB_2301751), mouse anti-Calbindin (SWANT C9638, RRID: AB_2314070).

    Techniques: Activity Assay, Incubation, Blocking Assay, Fluorescence

    The glucose transporter Glut4 is expressed in the brain and is present at nerve terminals .

    Journal: Neuron

    Article Title: Glut4 mobilization supports energetic demands of active synapses

    doi: 10.1016/j.neuron.2016.12.020

    Figure Lengend Snippet: The glucose transporter Glut4 is expressed in the brain and is present at nerve terminals .

    Article Snippet: The following primary antibodies were used: rabbit anti-Glut4 antibody (Alomone Labs AGT-024, RRID: AB_2631197), guinea pig anti-vGlut1 (Millipore AB5905, RRID: AB_2301751), mouse anti-Calbindin (SWANT C9638, RRID: AB_2314070).

    Techniques: