anti  (Alomone Labs)


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    Alomone Labs anti
    Anti, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti - by Bioz Stars, 2022-05
    91/100 stars

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    Alomone Labs anti glut3
    Sevoflurane reduces <t>GLUT3</t> protein expression in primary hippocampal neurons. a Primary hippocampal neurons were transfected with LV5-GLUT3. The transfection efficiency was examined by GFP expression (green) 36 h post infection, which was analysed by phase contrast (left panel) and fluorescence microscopy (right panel). b WB analysis of GLUT3 protein expression in primary hippocampal neurons 24 h after the last exposure to sevoflurane. Neurons were normally cultured (the Con group), only transfected with LV5-GLUT3 (the LV5-GLUT3 group), transfected with LV5-GLUT3 and then exposed to sevoflurane (the LV5-GLUT3 + Sev group), and only exposed to sevoflurane (the Sev group). c Immunofluorescence of GLUT3 expression in the somata and dendrites of primary hippocampal neurons 24 h after the last exposure to sevoflurane. The four groups: the LV5 group (neurons transfected with LV5 empty vectors), the LV5 + Sev group (neurons transfected with LV5 empty vectors and then exposed to sevoflurane), the LV5-GLUT3 and the LV5-GLUT3 + Sev group. d Histograms showing the results of GLUT3 blots in neurons among the four groups. n = 5 for each group. * P
    Anti Glut3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti glut3/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti glut3 - by Bioz Stars, 2022-05
    91/100 stars
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    Sevoflurane reduces GLUT3 protein expression in primary hippocampal neurons. a Primary hippocampal neurons were transfected with LV5-GLUT3. The transfection efficiency was examined by GFP expression (green) 36 h post infection, which was analysed by phase contrast (left panel) and fluorescence microscopy (right panel). b WB analysis of GLUT3 protein expression in primary hippocampal neurons 24 h after the last exposure to sevoflurane. Neurons were normally cultured (the Con group), only transfected with LV5-GLUT3 (the LV5-GLUT3 group), transfected with LV5-GLUT3 and then exposed to sevoflurane (the LV5-GLUT3 + Sev group), and only exposed to sevoflurane (the Sev group). c Immunofluorescence of GLUT3 expression in the somata and dendrites of primary hippocampal neurons 24 h after the last exposure to sevoflurane. The four groups: the LV5 group (neurons transfected with LV5 empty vectors), the LV5 + Sev group (neurons transfected with LV5 empty vectors and then exposed to sevoflurane), the LV5-GLUT3 and the LV5-GLUT3 + Sev group. d Histograms showing the results of GLUT3 blots in neurons among the four groups. n = 5 for each group. * P

    Journal: Cellular and Molecular Neurobiology

    Article Title: Sevoflurane Induces Learning and Memory Impairment in Young Mice Through a Reduction in Neuronal Glucose Transporter 3

    doi: 10.1007/s10571-019-00779-0

    Figure Lengend Snippet: Sevoflurane reduces GLUT3 protein expression in primary hippocampal neurons. a Primary hippocampal neurons were transfected with LV5-GLUT3. The transfection efficiency was examined by GFP expression (green) 36 h post infection, which was analysed by phase contrast (left panel) and fluorescence microscopy (right panel). b WB analysis of GLUT3 protein expression in primary hippocampal neurons 24 h after the last exposure to sevoflurane. Neurons were normally cultured (the Con group), only transfected with LV5-GLUT3 (the LV5-GLUT3 group), transfected with LV5-GLUT3 and then exposed to sevoflurane (the LV5-GLUT3 + Sev group), and only exposed to sevoflurane (the Sev group). c Immunofluorescence of GLUT3 expression in the somata and dendrites of primary hippocampal neurons 24 h after the last exposure to sevoflurane. The four groups: the LV5 group (neurons transfected with LV5 empty vectors), the LV5 + Sev group (neurons transfected with LV5 empty vectors and then exposed to sevoflurane), the LV5-GLUT3 and the LV5-GLUT3 + Sev group. d Histograms showing the results of GLUT3 blots in neurons among the four groups. n = 5 for each group. * P

    Article Snippet: The slides were incubated with anti-GLUT3 (1:150, AGT-023, Alomone) overnight at 4 °C before being washed three times with PBS and then incubated with the Cy3 goat anti-rabbit antibody (Aspen).

    Techniques: Expressing, Transfection, Infection, Fluorescence, Microscopy, Western Blot, Cell Culture, Immunofluorescence

    Sevoflurane inhibits GLUT3 surface expression and GLUT3 mRNA expression in neurons. a PC12 cells were transfected with LV5-GLUT3. The transfection efficiency was examined by GFP expression (green) 36 h post infection, which was analysed by phase contrast (left panel) and fluorescence microscopy (right panel). b The expression levels of GLUT3 protein in PC12 cells infected with LV5-GLUT3 were determined by WB analysis. PC12 cells were normally cultured (the Con group), transfected with LV5 empty vectors (the LV5 group), or transfected with LV5-GLUT3 (the LV5-GLUT3 group). c Flow cytometry analysis of GLUT3 surface expression in PC12 cells 24 h after the last exposure to sevoflurane. Four groups: the Con group, the Sev group (PC12 cells only exposed to sevoflurane), the LV5-GLUT3 group and the LV5-GLUT3 + Sev group (PC12 cells transfected with LV5-GLUT3 and then exposed to sevoflurane). n = 5 for each group. * P

    Journal: Cellular and Molecular Neurobiology

    Article Title: Sevoflurane Induces Learning and Memory Impairment in Young Mice Through a Reduction in Neuronal Glucose Transporter 3

    doi: 10.1007/s10571-019-00779-0

    Figure Lengend Snippet: Sevoflurane inhibits GLUT3 surface expression and GLUT3 mRNA expression in neurons. a PC12 cells were transfected with LV5-GLUT3. The transfection efficiency was examined by GFP expression (green) 36 h post infection, which was analysed by phase contrast (left panel) and fluorescence microscopy (right panel). b The expression levels of GLUT3 protein in PC12 cells infected with LV5-GLUT3 were determined by WB analysis. PC12 cells were normally cultured (the Con group), transfected with LV5 empty vectors (the LV5 group), or transfected with LV5-GLUT3 (the LV5-GLUT3 group). c Flow cytometry analysis of GLUT3 surface expression in PC12 cells 24 h after the last exposure to sevoflurane. Four groups: the Con group, the Sev group (PC12 cells only exposed to sevoflurane), the LV5-GLUT3 group and the LV5-GLUT3 + Sev group (PC12 cells transfected with LV5-GLUT3 and then exposed to sevoflurane). n = 5 for each group. * P

    Article Snippet: The slides were incubated with anti-GLUT3 (1:150, AGT-023, Alomone) overnight at 4 °C before being washed three times with PBS and then incubated with the Cy3 goat anti-rabbit antibody (Aspen).

    Techniques: Expressing, Transfection, Infection, Fluorescence, Microscopy, Western Blot, Cell Culture, Flow Cytometry

    Sevoflurane decreases GLUT3 protein expression in the hippocampus and temporal lobe. a WB analysis of GLUT1, GLUT4, and GLUT3 protein expression in the hippocampus and temporal lobe of young mice 24 h after the last exposure to sevoflurane. n = 4 for each group. b, c, d Histograms showing the quantification of GLUT1, GLUT4, and GLUT3 blots in the hippocampus and temporal lobe. In the hippocampus, * P

    Journal: Cellular and Molecular Neurobiology

    Article Title: Sevoflurane Induces Learning and Memory Impairment in Young Mice Through a Reduction in Neuronal Glucose Transporter 3

    doi: 10.1007/s10571-019-00779-0

    Figure Lengend Snippet: Sevoflurane decreases GLUT3 protein expression in the hippocampus and temporal lobe. a WB analysis of GLUT1, GLUT4, and GLUT3 protein expression in the hippocampus and temporal lobe of young mice 24 h after the last exposure to sevoflurane. n = 4 for each group. b, c, d Histograms showing the quantification of GLUT1, GLUT4, and GLUT3 blots in the hippocampus and temporal lobe. In the hippocampus, * P

    Article Snippet: The slides were incubated with anti-GLUT3 (1:150, AGT-023, Alomone) overnight at 4 °C before being washed three times with PBS and then incubated with the Cy3 goat anti-rabbit antibody (Aspen).

    Techniques: Expressing, Western Blot, Mouse Assay