anti glut1  (Alomone Labs)


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    Alomone Labs anti glut1
    Injection of Ad‐shGLUT2 into the 3V inhibits GLUT2 without altering GK and <t>GLUT1.</t> (a) Experimental protocol. (b‐d) Analysis of GLUT2, GK, and GLUT1 mRNA expression relative to cyclophilin in hypothalamic samples from rats transduced with Ad‐shβgal or Ad‐shGLUT2 by qRT‐PCR. (e) Western blot analysis of hypothalamic protein extracts isolated from rats transduced with Ad‐shβgal (lane 1) or Ad‐shGLUT2 (lane 2) for 48 hr. (f‐h) Densitometric analysis of each protein relative to β‐actin evaluated in Ad‐shGLUT2‐injected rats, as a percentage of levels observed in Ad‐shβgal‐EGFP‐injected rats. Results are representative of four independent experiments performed in triplicate. Data are expressed as mean ±SD. * p
    Anti Glut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti glut1/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti glut1 - by Bioz Stars, 2021-09
    90/100 stars

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    1) Product Images from "Glial hypothalamic inhibition of GLUT2 expression alters satiety, impacting eating behavior, et al. Glial hypothalamic inhibition of GLUT2 expression alters satiety, impacting eating behavior"

    Article Title: Glial hypothalamic inhibition of GLUT2 expression alters satiety, impacting eating behavior, et al. Glial hypothalamic inhibition of GLUT2 expression alters satiety, impacting eating behavior

    Journal: Glia

    doi: 10.1002/glia.23267

    Injection of Ad‐shGLUT2 into the 3V inhibits GLUT2 without altering GK and GLUT1. (a) Experimental protocol. (b‐d) Analysis of GLUT2, GK, and GLUT1 mRNA expression relative to cyclophilin in hypothalamic samples from rats transduced with Ad‐shβgal or Ad‐shGLUT2 by qRT‐PCR. (e) Western blot analysis of hypothalamic protein extracts isolated from rats transduced with Ad‐shβgal (lane 1) or Ad‐shGLUT2 (lane 2) for 48 hr. (f‐h) Densitometric analysis of each protein relative to β‐actin evaluated in Ad‐shGLUT2‐injected rats, as a percentage of levels observed in Ad‐shβgal‐EGFP‐injected rats. Results are representative of four independent experiments performed in triplicate. Data are expressed as mean ±SD. * p
    Figure Legend Snippet: Injection of Ad‐shGLUT2 into the 3V inhibits GLUT2 without altering GK and GLUT1. (a) Experimental protocol. (b‐d) Analysis of GLUT2, GK, and GLUT1 mRNA expression relative to cyclophilin in hypothalamic samples from rats transduced with Ad‐shβgal or Ad‐shGLUT2 by qRT‐PCR. (e) Western blot analysis of hypothalamic protein extracts isolated from rats transduced with Ad‐shβgal (lane 1) or Ad‐shGLUT2 (lane 2) for 48 hr. (f‐h) Densitometric analysis of each protein relative to β‐actin evaluated in Ad‐shGLUT2‐injected rats, as a percentage of levels observed in Ad‐shβgal‐EGFP‐injected rats. Results are representative of four independent experiments performed in triplicate. Data are expressed as mean ±SD. * p

    Techniques Used: Injection, Expressing, Transduction, Quantitative RT-PCR, Western Blot, Isolation

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    Alomone Labs anti glut1
    Injection of Ad‐shGLUT2 into the 3V inhibits GLUT2 without altering GK and <t>GLUT1.</t> (a) Experimental protocol. (b‐d) Analysis of GLUT2, GK, and GLUT1 mRNA expression relative to cyclophilin in hypothalamic samples from rats transduced with Ad‐shβgal or Ad‐shGLUT2 by qRT‐PCR. (e) Western blot analysis of hypothalamic protein extracts isolated from rats transduced with Ad‐shβgal (lane 1) or Ad‐shGLUT2 (lane 2) for 48 hr. (f‐h) Densitometric analysis of each protein relative to β‐actin evaluated in Ad‐shGLUT2‐injected rats, as a percentage of levels observed in Ad‐shβgal‐EGFP‐injected rats. Results are representative of four independent experiments performed in triplicate. Data are expressed as mean ±SD. * p
    Anti Glut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti glut1/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti glut1 - by Bioz Stars, 2021-09
    90/100 stars
      Buy from Supplier

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    Injection of Ad‐shGLUT2 into the 3V inhibits GLUT2 without altering GK and GLUT1. (a) Experimental protocol. (b‐d) Analysis of GLUT2, GK, and GLUT1 mRNA expression relative to cyclophilin in hypothalamic samples from rats transduced with Ad‐shβgal or Ad‐shGLUT2 by qRT‐PCR. (e) Western blot analysis of hypothalamic protein extracts isolated from rats transduced with Ad‐shβgal (lane 1) or Ad‐shGLUT2 (lane 2) for 48 hr. (f‐h) Densitometric analysis of each protein relative to β‐actin evaluated in Ad‐shGLUT2‐injected rats, as a percentage of levels observed in Ad‐shβgal‐EGFP‐injected rats. Results are representative of four independent experiments performed in triplicate. Data are expressed as mean ±SD. * p

    Journal: Glia

    Article Title: Glial hypothalamic inhibition of GLUT2 expression alters satiety, impacting eating behavior, et al. Glial hypothalamic inhibition of GLUT2 expression alters satiety, impacting eating behavior

    doi: 10.1002/glia.23267

    Figure Lengend Snippet: Injection of Ad‐shGLUT2 into the 3V inhibits GLUT2 without altering GK and GLUT1. (a) Experimental protocol. (b‐d) Analysis of GLUT2, GK, and GLUT1 mRNA expression relative to cyclophilin in hypothalamic samples from rats transduced with Ad‐shβgal or Ad‐shGLUT2 by qRT‐PCR. (e) Western blot analysis of hypothalamic protein extracts isolated from rats transduced with Ad‐shβgal (lane 1) or Ad‐shGLUT2 (lane 2) for 48 hr. (f‐h) Densitometric analysis of each protein relative to β‐actin evaluated in Ad‐shGLUT2‐injected rats, as a percentage of levels observed in Ad‐shβgal‐EGFP‐injected rats. Results are representative of four independent experiments performed in triplicate. Data are expressed as mean ±SD. * p

    Article Snippet: Samples were homogenized in buffer A (0.3 mM sucrose, 3 mM DTT, 1 mM EDTA, 100 µg/ml PMSF, 2 µg/ml pepstatin A, 2 µg/ml leucopeptin, and 2 mg/ml aprotinin), sonicated three times on ice at 60 Hz for 10 s (Sonics & Material INC, VCF1, Newtown, CT, USA), and separated by centrifugation at 8,000g for 10 min. Proteins were resolved by SDS‐PAGE (50 µg/lane) in a 12% (w/v) polyacrylamide gel, transferred to PVDF membranes (0.45 mm pore, Amersham Pharmacia Biotech., Piscataway, NJ, USA), and probed for 16 hr at 4°C with rabbit anti‐GLUT2 (1:1,000; Alpha Diagnostic, San Antonio, TX, USA), anti‐GLUT1 (1:1,000; Alomone Labs, Jerusalem, Israel), anti‐glucokinase (GK; 1:2,000; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and anti‐β‐actin (1:10,000; Santa Cruz).

    Techniques: Injection, Expressing, Transduction, Quantitative RT-PCR, Western Blot, Isolation