ncx3  (Alomone Labs)


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    Name:
    Anti NCX3 SLC8A3 Antibody
    Description:
    Anti NCX3 SLC8A3 Antibody is directed against an epitope of the rat Na Ca2 exchange protein 3 Anti NCX3 SLC8A3 Antibody ANX 013 can be used in western blot and immunohistochemistry applications It has been designed to recognize NCX3 from human rat and mouse samples
    Catalog Number:
    ANX-013
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunofluorescence, Immunohistochemistry, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs ncx3
    Anti NCX3 SLC8A3 Antibody
    Anti NCX3 SLC8A3 Antibody is directed against an epitope of the rat Na Ca2 exchange protein 3 Anti NCX3 SLC8A3 Antibody ANX 013 can be used in western blot and immunohistochemistry applications It has been designed to recognize NCX3 from human rat and mouse samples
    https://www.bioz.com/result/ncx3/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ncx3 - by Bioz Stars, 2021-09
    92/100 stars

    Images

    1) Product Images from "Blockade of the forward Na+/Ca2+ exchanger suppresses the growth of glioblastoma cells through Ca2+‐mediated cell death, et al. Blockade of the forward Na+/Ca2+ exchanger suppresses the growth of glioblastoma cells through Ca2+‐mediated cell death"

    Article Title: Blockade of the forward Na+/Ca2+ exchanger suppresses the growth of glioblastoma cells through Ca2+‐mediated cell death, et al. Blockade of the forward Na+/Ca2+ exchanger suppresses the growth of glioblastoma cells through Ca2+‐mediated cell death

    Journal: British Journal of Pharmacology

    doi: 10.1111/bph.14692

    Impact of bepridil on human astrocytes (HA) and the Na + /Ca 2+ exchanger (NCX) isoforms in each cell line. (a) Viability of U87, U251, and SF188 cells and HA after exposure to bepridil (25 μM, 48 hr); n = 5 independent tests in each group. (b) The bands of NCX1, NCX2, and NCX3 isoforms detected by Western blot analysis in the sample of HA and glioblastoma cell lines U87, U118, A172, U251, and SF188. (c) Representative recording of the NCX currents in HA and glioblastoma cell lines. (d) Viability of U87, U251, and SF188 cells and HA after exposure to KB‐R7943 (25 μM, 48 hr). n = 5 independent tests in each group
    Figure Legend Snippet: Impact of bepridil on human astrocytes (HA) and the Na + /Ca 2+ exchanger (NCX) isoforms in each cell line. (a) Viability of U87, U251, and SF188 cells and HA after exposure to bepridil (25 μM, 48 hr); n = 5 independent tests in each group. (b) The bands of NCX1, NCX2, and NCX3 isoforms detected by Western blot analysis in the sample of HA and glioblastoma cell lines U87, U118, A172, U251, and SF188. (c) Representative recording of the NCX currents in HA and glioblastoma cell lines. (d) Viability of U87, U251, and SF188 cells and HA after exposure to KB‐R7943 (25 μM, 48 hr). n = 5 independent tests in each group

    Techniques Used: Western Blot

    2) Product Images from "Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis"

    Article Title: Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis

    Journal: Experimental & Molecular Medicine

    doi: 10.1038/s12276-018-0088-z

    Na + /Ca 2+ exchanger (NCX) expression levels were significantly increased and the reverse mode of NCX was relatively activated in bladder ICC-LCs from CYP-8d rats. a – c The mRNA and protein expression levels of NCX1 and NCX3 were significantly increased in CYP-48h and CYP-8d rat bladders compared with those in naive and CYP-4h rat bladders, and increases in CYP-8d rat bladders were more significant; the mRNA and protein expression levels of NCX2 were only increased in CYP-8d rat bladders. d Changes in NCX1 expression (red) in bladder c-kit (green)-positive ICC-LCs (white arrows) were detected using IF staining. Bladder ICC-LCs from CYP-8d rats possessed higher NCX1 protein expression than those from naive rats. e , f NCX current (I NCX ) in isolated bladder ICC-LCs from naive and CYP-8d rats was evoked by a step potential ranging from −100 to +60 mV in increments of 10 mV for 200 ms, with a holding potential of −40 mV. The forward and reverse modes of NCX were elicited over a voltage range of −100 to −50 mV and −30 to +60 mV, respectively, and are exhibited as the bottom and upper part of the characteristic I NCX traces. When normalized to cell capacitance, the absolute value of the I NCX density in bladder ICC-LCs from CYP-8d rats significantly exceeded that in bladder ICC-LCs from naive rats over a voltage range of −100 to −80 mV (forward mode) and +10 to +60 mV (reverse mode). Notably, the increase in the reverse mode of NCX was more significant (the data represent the mean ± S.D., n = 8; * P
    Figure Legend Snippet: Na + /Ca 2+ exchanger (NCX) expression levels were significantly increased and the reverse mode of NCX was relatively activated in bladder ICC-LCs from CYP-8d rats. a – c The mRNA and protein expression levels of NCX1 and NCX3 were significantly increased in CYP-48h and CYP-8d rat bladders compared with those in naive and CYP-4h rat bladders, and increases in CYP-8d rat bladders were more significant; the mRNA and protein expression levels of NCX2 were only increased in CYP-8d rat bladders. d Changes in NCX1 expression (red) in bladder c-kit (green)-positive ICC-LCs (white arrows) were detected using IF staining. Bladder ICC-LCs from CYP-8d rats possessed higher NCX1 protein expression than those from naive rats. e , f NCX current (I NCX ) in isolated bladder ICC-LCs from naive and CYP-8d rats was evoked by a step potential ranging from −100 to +60 mV in increments of 10 mV for 200 ms, with a holding potential of −40 mV. The forward and reverse modes of NCX were elicited over a voltage range of −100 to −50 mV and −30 to +60 mV, respectively, and are exhibited as the bottom and upper part of the characteristic I NCX traces. When normalized to cell capacitance, the absolute value of the I NCX density in bladder ICC-LCs from CYP-8d rats significantly exceeded that in bladder ICC-LCs from naive rats over a voltage range of −100 to −80 mV (forward mode) and +10 to +60 mV (reverse mode). Notably, the increase in the reverse mode of NCX was more significant (the data represent the mean ± S.D., n = 8; * P

    Techniques Used: Expressing, Immunocytochemistry, Staining, Isolation, Mass Spectrometry

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    Alomone Labs ncx3
    Impact of bepridil on human astrocytes (HA) and the Na + /Ca 2+ exchanger (NCX) isoforms in each cell line. (a) Viability of U87, U251, and SF188 cells and HA after exposure to bepridil (25 μM, 48 hr); n = 5 independent tests in each group. (b) The bands of NCX1, NCX2, and <t>NCX3</t> isoforms detected by Western blot analysis in the sample of HA and glioblastoma cell lines U87, U118, A172, U251, and SF188. (c) Representative recording of the NCX currents in HA and glioblastoma cell lines. (d) Viability of U87, U251, and SF188 cells and HA after exposure to KB‐R7943 (25 μM, 48 hr). n = 5 independent tests in each group
    Ncx3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncx3/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ncx3 - by Bioz Stars, 2021-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Impact of bepridil on human astrocytes (HA) and the Na + /Ca 2+ exchanger (NCX) isoforms in each cell line. (a) Viability of U87, U251, and SF188 cells and HA after exposure to bepridil (25 μM, 48 hr); n = 5 independent tests in each group. (b) The bands of NCX1, NCX2, and NCX3 isoforms detected by Western blot analysis in the sample of HA and glioblastoma cell lines U87, U118, A172, U251, and SF188. (c) Representative recording of the NCX currents in HA and glioblastoma cell lines. (d) Viability of U87, U251, and SF188 cells and HA after exposure to KB‐R7943 (25 μM, 48 hr). n = 5 independent tests in each group

    Journal: British Journal of Pharmacology

    Article Title: Blockade of the forward Na+/Ca2+ exchanger suppresses the growth of glioblastoma cells through Ca2+‐mediated cell death, et al. Blockade of the forward Na+/Ca2+ exchanger suppresses the growth of glioblastoma cells through Ca2+‐mediated cell death

    doi: 10.1111/bph.14692

    Figure Lengend Snippet: Impact of bepridil on human astrocytes (HA) and the Na + /Ca 2+ exchanger (NCX) isoforms in each cell line. (a) Viability of U87, U251, and SF188 cells and HA after exposure to bepridil (25 μM, 48 hr); n = 5 independent tests in each group. (b) The bands of NCX1, NCX2, and NCX3 isoforms detected by Western blot analysis in the sample of HA and glioblastoma cell lines U87, U118, A172, U251, and SF188. (c) Representative recording of the NCX currents in HA and glioblastoma cell lines. (d) Viability of U87, U251, and SF188 cells and HA after exposure to KB‐R7943 (25 μM, 48 hr). n = 5 independent tests in each group

    Article Snippet: Anti‐NCX1 antibody (Cat# ab177952, RRID:AB_2801276) was purchased from Abcam (Cambridge, MA, USA), and anti‐NCX2 (Cat# ANX‐012, RRID:AB_2341022) and NCX3 (Cat# ANX‐013, RRID:AB_2341023) antibodies were purchased from Alomone Labs (Jerusalem, Israel). http://www.jkchemical.com/CH/products/A01433521.html J & K Scientific (Sunnyvale, CA, USA).

    Techniques: Western Blot