anti glyt2 slc6a5 extracellular antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs anti glyt2 slc6a5 extracellular antibody
    Glycinergic neuronal cell bodies near the the central canal of the avian lumbosacral organ (LSO) display myosin7a immunoreactivity. (A) The synsacrum (fused to the pelvic bones) is highlighted within a reconstructed CT scan of the zebra finch axial skeleton (Yale Peabody Museum specimen #125076). (B) Dorsal and ventral views of the synsacrum, reconstructed from the CT scan. (C) A sagittal section through the vertebral canal of the synsacrum CT scan shows the dorsal expansion of the vertebral canal that holds the glycogen body. A parasagittal section shows transverse canal-like structures on the internal dorsal side of the vertebral canal that roughly align with the accessory lobes (see [ 16 ]). (D) A diagram of the neural tissue of the LSO based on dissection and histology observations and a contrast-enhanced CT scan (data from [ 16 ]). (E) Cells near the central canal (middle) that are immunoreactive for both <t>glyt2</t> and myo7a. (F) Higher-resolution image of the right cell from (E). (G) Labeling with two different myo7a antibodies (mouse and rabbit). (H) neun/fox3 labeling within the myo7a+ cells. White arrow in the first panel of subfigure (E) and (G) point to the ependymal layer surrounding the central canal. Upper right diagram in the first panel of subfigures (E-H) indicates approximately where in the transverse section the image was taken.
    Anti Glyt2 Slc6a5 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti glyt2 slc6a5 extracellular antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti glyt2 slc6a5 extracellular antibody - by Bioz Stars, 2022-08
    94/100 stars

    Images

    1) Product Images from "Molecular markers of mechanosensation in glycinergic neurons in the avian lumbosacral spinal cord"

    Article Title: Molecular markers of mechanosensation in glycinergic neurons in the avian lumbosacral spinal cord

    Journal: bioRxiv

    doi: 10.1101/2022.01.28.478253

    Glycinergic neuronal cell bodies near the the central canal of the avian lumbosacral organ (LSO) display myosin7a immunoreactivity. (A) The synsacrum (fused to the pelvic bones) is highlighted within a reconstructed CT scan of the zebra finch axial skeleton (Yale Peabody Museum specimen #125076). (B) Dorsal and ventral views of the synsacrum, reconstructed from the CT scan. (C) A sagittal section through the vertebral canal of the synsacrum CT scan shows the dorsal expansion of the vertebral canal that holds the glycogen body. A parasagittal section shows transverse canal-like structures on the internal dorsal side of the vertebral canal that roughly align with the accessory lobes (see [ 16 ]). (D) A diagram of the neural tissue of the LSO based on dissection and histology observations and a contrast-enhanced CT scan (data from [ 16 ]). (E) Cells near the central canal (middle) that are immunoreactive for both glyt2 and myo7a. (F) Higher-resolution image of the right cell from (E). (G) Labeling with two different myo7a antibodies (mouse and rabbit). (H) neun/fox3 labeling within the myo7a+ cells. White arrow in the first panel of subfigure (E) and (G) point to the ependymal layer surrounding the central canal. Upper right diagram in the first panel of subfigures (E-H) indicates approximately where in the transverse section the image was taken.
    Figure Legend Snippet: Glycinergic neuronal cell bodies near the the central canal of the avian lumbosacral organ (LSO) display myosin7a immunoreactivity. (A) The synsacrum (fused to the pelvic bones) is highlighted within a reconstructed CT scan of the zebra finch axial skeleton (Yale Peabody Museum specimen #125076). (B) Dorsal and ventral views of the synsacrum, reconstructed from the CT scan. (C) A sagittal section through the vertebral canal of the synsacrum CT scan shows the dorsal expansion of the vertebral canal that holds the glycogen body. A parasagittal section shows transverse canal-like structures on the internal dorsal side of the vertebral canal that roughly align with the accessory lobes (see [ 16 ]). (D) A diagram of the neural tissue of the LSO based on dissection and histology observations and a contrast-enhanced CT scan (data from [ 16 ]). (E) Cells near the central canal (middle) that are immunoreactive for both glyt2 and myo7a. (F) Higher-resolution image of the right cell from (E). (G) Labeling with two different myo7a antibodies (mouse and rabbit). (H) neun/fox3 labeling within the myo7a+ cells. White arrow in the first panel of subfigure (E) and (G) point to the ependymal layer surrounding the central canal. Upper right diagram in the first panel of subfigures (E-H) indicates approximately where in the transverse section the image was taken.

    Techniques Used: Computed Tomography, Dissection, Labeling

    Lateral glycinergic processes are immunoreactive for proteins also present in inner ear hair cell stereocilia. (A) Glyt2+ ventrolateral processes are immunoreactive for myo7a. Margin of spinal cord with dentate ligament is at lower left; upper left is accessory lobe. (B C) The accessory lobe processes (B) and terminations at the dentate ligament (C) double-label with two different antibodies for myo7a (mouse and rabbit). (D) Myo7a-immunoreactive processes in the accessory lobe also react with anti-espin. (AD) In composite images, blue is DAPI. Upper right diagram in the first panel of each subfigure indicates approximately where in the transverse section the image was taken.
    Figure Legend Snippet: Lateral glycinergic processes are immunoreactive for proteins also present in inner ear hair cell stereocilia. (A) Glyt2+ ventrolateral processes are immunoreactive for myo7a. Margin of spinal cord with dentate ligament is at lower left; upper left is accessory lobe. (B C) The accessory lobe processes (B) and terminations at the dentate ligament (C) double-label with two different antibodies for myo7a (mouse and rabbit). (D) Myo7a-immunoreactive processes in the accessory lobe also react with anti-espin. (AD) In composite images, blue is DAPI. Upper right diagram in the first panel of each subfigure indicates approximately where in the transverse section the image was taken.

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    Alomone Labs anti glyt2 slc6a5 extracellular antibody
    Glycinergic neuronal cell bodies near the the central canal of the avian lumbosacral organ (LSO) display myosin7a immunoreactivity. (A) The synsacrum (fused to the pelvic bones) is highlighted within a reconstructed CT scan of the zebra finch axial skeleton (Yale Peabody Museum specimen #125076). (B) Dorsal and ventral views of the synsacrum, reconstructed from the CT scan. (C) A sagittal section through the vertebral canal of the synsacrum CT scan shows the dorsal expansion of the vertebral canal that holds the glycogen body. A parasagittal section shows transverse canal-like structures on the internal dorsal side of the vertebral canal that roughly align with the accessory lobes (see [ 16 ]). (D) A diagram of the neural tissue of the LSO based on dissection and histology observations and a contrast-enhanced CT scan (data from [ 16 ]). (E) Cells near the central canal (middle) that are immunoreactive for both <t>glyt2</t> and myo7a. (F) Higher-resolution image of the right cell from (E). (G) Labeling with two different myo7a antibodies (mouse and rabbit). (H) neun/fox3 labeling within the myo7a+ cells. White arrow in the first panel of subfigure (E) and (G) point to the ependymal layer surrounding the central canal. Upper right diagram in the first panel of subfigures (E-H) indicates approximately where in the transverse section the image was taken.
    Anti Glyt2 Slc6a5 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti glyt2 slc6a5 extracellular antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti glyt2 slc6a5 extracellular antibody - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

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    Glycinergic neuronal cell bodies near the the central canal of the avian lumbosacral organ (LSO) display myosin7a immunoreactivity. (A) The synsacrum (fused to the pelvic bones) is highlighted within a reconstructed CT scan of the zebra finch axial skeleton (Yale Peabody Museum specimen #125076). (B) Dorsal and ventral views of the synsacrum, reconstructed from the CT scan. (C) A sagittal section through the vertebral canal of the synsacrum CT scan shows the dorsal expansion of the vertebral canal that holds the glycogen body. A parasagittal section shows transverse canal-like structures on the internal dorsal side of the vertebral canal that roughly align with the accessory lobes (see [ 16 ]). (D) A diagram of the neural tissue of the LSO based on dissection and histology observations and a contrast-enhanced CT scan (data from [ 16 ]). (E) Cells near the central canal (middle) that are immunoreactive for both glyt2 and myo7a. (F) Higher-resolution image of the right cell from (E). (G) Labeling with two different myo7a antibodies (mouse and rabbit). (H) neun/fox3 labeling within the myo7a+ cells. White arrow in the first panel of subfigure (E) and (G) point to the ependymal layer surrounding the central canal. Upper right diagram in the first panel of subfigures (E-H) indicates approximately where in the transverse section the image was taken.

    Journal: bioRxiv

    Article Title: Molecular markers of mechanosensation in glycinergic neurons in the avian lumbosacral spinal cord

    doi: 10.1101/2022.01.28.478253

    Figure Lengend Snippet: Glycinergic neuronal cell bodies near the the central canal of the avian lumbosacral organ (LSO) display myosin7a immunoreactivity. (A) The synsacrum (fused to the pelvic bones) is highlighted within a reconstructed CT scan of the zebra finch axial skeleton (Yale Peabody Museum specimen #125076). (B) Dorsal and ventral views of the synsacrum, reconstructed from the CT scan. (C) A sagittal section through the vertebral canal of the synsacrum CT scan shows the dorsal expansion of the vertebral canal that holds the glycogen body. A parasagittal section shows transverse canal-like structures on the internal dorsal side of the vertebral canal that roughly align with the accessory lobes (see [ 16 ]). (D) A diagram of the neural tissue of the LSO based on dissection and histology observations and a contrast-enhanced CT scan (data from [ 16 ]). (E) Cells near the central canal (middle) that are immunoreactive for both glyt2 and myo7a. (F) Higher-resolution image of the right cell from (E). (G) Labeling with two different myo7a antibodies (mouse and rabbit). (H) neun/fox3 labeling within the myo7a+ cells. White arrow in the first panel of subfigure (E) and (G) point to the ependymal layer surrounding the central canal. Upper right diagram in the first panel of subfigures (E-H) indicates approximately where in the transverse section the image was taken.

    Article Snippet: Primary Abs used were rabbit-antiglyt2 (Alomone AGT-012; 1:400 n=3 or 1:200 n=1; 13/14 immunogen amino acids identical to T. guttata glyt2 according to BLAST-P search), mouse-antimyo7a (DSHB 138-1, 1:100 n=3 or 1:200 n=4; avian cross-reactivity listed by manufacturer), rabbit-antimyo7a (Proteus 25-6790, 1:400 n=3 1:500 n=2; avian cross-reactivity listed by manufacturer), rabbit-antiespin (Invitrogen PA5-55941, 1:200 n=5; labeled interior side of ependymal cells in T. guttata spinal cord, where espin+ apical tips of CSF-cNs are located; 54/64 immunogen amino acids identical to T. guttata espin according to BLAST-P search), rabbit-antineun/fox3 (Invitrogen 702022; 1:200 n=3 or 1:500 n=2; labeled cells in spinal cord as expected).

    Techniques: Computed Tomography, Dissection, Labeling

    Lateral glycinergic processes are immunoreactive for proteins also present in inner ear hair cell stereocilia. (A) Glyt2+ ventrolateral processes are immunoreactive for myo7a. Margin of spinal cord with dentate ligament is at lower left; upper left is accessory lobe. (B C) The accessory lobe processes (B) and terminations at the dentate ligament (C) double-label with two different antibodies for myo7a (mouse and rabbit). (D) Myo7a-immunoreactive processes in the accessory lobe also react with anti-espin. (AD) In composite images, blue is DAPI. Upper right diagram in the first panel of each subfigure indicates approximately where in the transverse section the image was taken.

    Journal: bioRxiv

    Article Title: Molecular markers of mechanosensation in glycinergic neurons in the avian lumbosacral spinal cord

    doi: 10.1101/2022.01.28.478253

    Figure Lengend Snippet: Lateral glycinergic processes are immunoreactive for proteins also present in inner ear hair cell stereocilia. (A) Glyt2+ ventrolateral processes are immunoreactive for myo7a. Margin of spinal cord with dentate ligament is at lower left; upper left is accessory lobe. (B C) The accessory lobe processes (B) and terminations at the dentate ligament (C) double-label with two different antibodies for myo7a (mouse and rabbit). (D) Myo7a-immunoreactive processes in the accessory lobe also react with anti-espin. (AD) In composite images, blue is DAPI. Upper right diagram in the first panel of each subfigure indicates approximately where in the transverse section the image was taken.

    Article Snippet: Primary Abs used were rabbit-antiglyt2 (Alomone AGT-012; 1:400 n=3 or 1:200 n=1; 13/14 immunogen amino acids identical to T. guttata glyt2 according to BLAST-P search), mouse-antimyo7a (DSHB 138-1, 1:100 n=3 or 1:200 n=4; avian cross-reactivity listed by manufacturer), rabbit-antimyo7a (Proteus 25-6790, 1:400 n=3 1:500 n=2; avian cross-reactivity listed by manufacturer), rabbit-antiespin (Invitrogen PA5-55941, 1:200 n=5; labeled interior side of ependymal cells in T. guttata spinal cord, where espin+ apical tips of CSF-cNs are located; 54/64 immunogen amino acids identical to T. guttata espin according to BLAST-P search), rabbit-antineun/fox3 (Invitrogen 702022; 1:200 n=3 or 1:500 n=2; labeled cells in spinal cord as expected).

    Techniques: