ncx2  (Alomone Labs)


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    Structured Review

    Alomone Labs ncx2
    Na + /Ca 2+ exchanger (NCX) expression levels were significantly increased and the reverse mode of NCX was relatively activated in bladder ICC-LCs from CYP-8d rats. a – c The mRNA and protein expression levels of NCX1 and NCX3 were significantly increased in CYP-48h and CYP-8d rat bladders compared with those in naive and CYP-4h rat bladders, and increases in CYP-8d rat bladders were more significant; the mRNA and protein expression levels of <t>NCX2</t> were only increased in CYP-8d rat bladders. d Changes in NCX1 expression (red) in bladder c-kit (green)-positive ICC-LCs (white arrows) were detected using IF staining. Bladder ICC-LCs from CYP-8d rats possessed higher NCX1 protein expression than those from naive rats. e , f NCX current (I NCX ) in isolated bladder ICC-LCs from naive and CYP-8d rats was evoked by a step potential ranging from −100 to +60 mV in increments of 10 mV for 200 ms, with a holding potential of −40 mV. The forward and reverse modes of NCX were elicited over a voltage range of −100 to −50 mV and −30 to +60 mV, respectively, and are exhibited as the bottom and upper part of the characteristic I NCX traces. When normalized to cell capacitance, the absolute value of the I NCX density in bladder ICC-LCs from CYP-8d rats significantly exceeded that in bladder ICC-LCs from naive rats over a voltage range of −100 to −80 mV (forward mode) and +10 to +60 mV (reverse mode). Notably, the increase in the reverse mode of NCX was more significant (the data represent the mean ± S.D., n = 8; * P
    Ncx2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncx2/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
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    Images

    1) Product Images from "Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis"

    Article Title: Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis

    Journal: Experimental & Molecular Medicine

    doi: 10.1038/s12276-018-0088-z

    Na + /Ca 2+ exchanger (NCX) expression levels were significantly increased and the reverse mode of NCX was relatively activated in bladder ICC-LCs from CYP-8d rats. a – c The mRNA and protein expression levels of NCX1 and NCX3 were significantly increased in CYP-48h and CYP-8d rat bladders compared with those in naive and CYP-4h rat bladders, and increases in CYP-8d rat bladders were more significant; the mRNA and protein expression levels of NCX2 were only increased in CYP-8d rat bladders. d Changes in NCX1 expression (red) in bladder c-kit (green)-positive ICC-LCs (white arrows) were detected using IF staining. Bladder ICC-LCs from CYP-8d rats possessed higher NCX1 protein expression than those from naive rats. e , f NCX current (I NCX ) in isolated bladder ICC-LCs from naive and CYP-8d rats was evoked by a step potential ranging from −100 to +60 mV in increments of 10 mV for 200 ms, with a holding potential of −40 mV. The forward and reverse modes of NCX were elicited over a voltage range of −100 to −50 mV and −30 to +60 mV, respectively, and are exhibited as the bottom and upper part of the characteristic I NCX traces. When normalized to cell capacitance, the absolute value of the I NCX density in bladder ICC-LCs from CYP-8d rats significantly exceeded that in bladder ICC-LCs from naive rats over a voltage range of −100 to −80 mV (forward mode) and +10 to +60 mV (reverse mode). Notably, the increase in the reverse mode of NCX was more significant (the data represent the mean ± S.D., n = 8; * P
    Figure Legend Snippet: Na + /Ca 2+ exchanger (NCX) expression levels were significantly increased and the reverse mode of NCX was relatively activated in bladder ICC-LCs from CYP-8d rats. a – c The mRNA and protein expression levels of NCX1 and NCX3 were significantly increased in CYP-48h and CYP-8d rat bladders compared with those in naive and CYP-4h rat bladders, and increases in CYP-8d rat bladders were more significant; the mRNA and protein expression levels of NCX2 were only increased in CYP-8d rat bladders. d Changes in NCX1 expression (red) in bladder c-kit (green)-positive ICC-LCs (white arrows) were detected using IF staining. Bladder ICC-LCs from CYP-8d rats possessed higher NCX1 protein expression than those from naive rats. e , f NCX current (I NCX ) in isolated bladder ICC-LCs from naive and CYP-8d rats was evoked by a step potential ranging from −100 to +60 mV in increments of 10 mV for 200 ms, with a holding potential of −40 mV. The forward and reverse modes of NCX were elicited over a voltage range of −100 to −50 mV and −30 to +60 mV, respectively, and are exhibited as the bottom and upper part of the characteristic I NCX traces. When normalized to cell capacitance, the absolute value of the I NCX density in bladder ICC-LCs from CYP-8d rats significantly exceeded that in bladder ICC-LCs from naive rats over a voltage range of −100 to −80 mV (forward mode) and +10 to +60 mV (reverse mode). Notably, the increase in the reverse mode of NCX was more significant (the data represent the mean ± S.D., n = 8; * P

    Techniques Used: Expressing, Immunocytochemistry, Staining, Isolation, Mass Spectrometry

    Related Articles

    Incubation:

    Article Title: Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis
    Article Snippet: .. After being blocked with 5% skim milk dissolved in Tris-buffered saline at room temperature for 2 h, the membranes were incubated overnight at 4 °C with various primary antibodies targeting the following proteins: IL-6 (Abcam, Cambridge, UK, ab9324, 1:800), TNF-α (Abcam, ab199013, 1:500), Piezo1 (Alomone labs, Jerusalem, Israel, APC-087, 1:500), NCX1 (Abcam, ab2869, 1:800), NCX2 (Alomone labs, ANX-012, 1:500), NCX3 (Alomone labs, ANX-013, 1:500), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Beyotime, AG019, 1:1000), and α-tubulin (Beyotime, AT819, 1:1000). ..

    other:

    Article Title: The TRPC channel blocker SKF 96365 inhibits glioblastoma cell growth by enhancing reverse mode of the Na+/Ca2+ exchanger and increasing intracellular Ca2+
    Article Snippet: Anti-NCX1 antibody was purchased from Abcam, Inc. (Cambridge, MA, USA); anti-NCX2 and NCX3 antibodies were purchased from Alomone Labs (Jerusalem, Israel).

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    Alomone Labs ncx2
    Na + /Ca 2+ exchanger (NCX) expression levels were significantly increased and the reverse mode of NCX was relatively activated in bladder ICC-LCs from CYP-8d rats. a – c The mRNA and protein expression levels of NCX1 and NCX3 were significantly increased in CYP-48h and CYP-8d rat bladders compared with those in naive and CYP-4h rat bladders, and increases in CYP-8d rat bladders were more significant; the mRNA and protein expression levels of <t>NCX2</t> were only increased in CYP-8d rat bladders. d Changes in NCX1 expression (red) in bladder c-kit (green)-positive ICC-LCs (white arrows) were detected using IF staining. Bladder ICC-LCs from CYP-8d rats possessed higher NCX1 protein expression than those from naive rats. e , f NCX current (I NCX ) in isolated bladder ICC-LCs from naive and CYP-8d rats was evoked by a step potential ranging from −100 to +60 mV in increments of 10 mV for 200 ms, with a holding potential of −40 mV. The forward and reverse modes of NCX were elicited over a voltage range of −100 to −50 mV and −30 to +60 mV, respectively, and are exhibited as the bottom and upper part of the characteristic I NCX traces. When normalized to cell capacitance, the absolute value of the I NCX density in bladder ICC-LCs from CYP-8d rats significantly exceeded that in bladder ICC-LCs from naive rats over a voltage range of −100 to −80 mV (forward mode) and +10 to +60 mV (reverse mode). Notably, the increase in the reverse mode of NCX was more significant (the data represent the mean ± S.D., n = 8; * P
    Ncx2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncx2/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ncx2 - by Bioz Stars, 2021-09
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      Buy from Supplier

    94
    Alomone Labs asic2a
    Parkin suppresses PICK1-mediated potentiation of <t>ASIC2a</t> currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after
    Asic2a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    asic2a - by Bioz Stars, 2021-09
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    Image Search Results


    Na + /Ca 2+ exchanger (NCX) expression levels were significantly increased and the reverse mode of NCX was relatively activated in bladder ICC-LCs from CYP-8d rats. a – c The mRNA and protein expression levels of NCX1 and NCX3 were significantly increased in CYP-48h and CYP-8d rat bladders compared with those in naive and CYP-4h rat bladders, and increases in CYP-8d rat bladders were more significant; the mRNA and protein expression levels of NCX2 were only increased in CYP-8d rat bladders. d Changes in NCX1 expression (red) in bladder c-kit (green)-positive ICC-LCs (white arrows) were detected using IF staining. Bladder ICC-LCs from CYP-8d rats possessed higher NCX1 protein expression than those from naive rats. e , f NCX current (I NCX ) in isolated bladder ICC-LCs from naive and CYP-8d rats was evoked by a step potential ranging from −100 to +60 mV in increments of 10 mV for 200 ms, with a holding potential of −40 mV. The forward and reverse modes of NCX were elicited over a voltage range of −100 to −50 mV and −30 to +60 mV, respectively, and are exhibited as the bottom and upper part of the characteristic I NCX traces. When normalized to cell capacitance, the absolute value of the I NCX density in bladder ICC-LCs from CYP-8d rats significantly exceeded that in bladder ICC-LCs from naive rats over a voltage range of −100 to −80 mV (forward mode) and +10 to +60 mV (reverse mode). Notably, the increase in the reverse mode of NCX was more significant (the data represent the mean ± S.D., n = 8; * P

    Journal: Experimental & Molecular Medicine

    Article Title: Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis

    doi: 10.1038/s12276-018-0088-z

    Figure Lengend Snippet: Na + /Ca 2+ exchanger (NCX) expression levels were significantly increased and the reverse mode of NCX was relatively activated in bladder ICC-LCs from CYP-8d rats. a – c The mRNA and protein expression levels of NCX1 and NCX3 were significantly increased in CYP-48h and CYP-8d rat bladders compared with those in naive and CYP-4h rat bladders, and increases in CYP-8d rat bladders were more significant; the mRNA and protein expression levels of NCX2 were only increased in CYP-8d rat bladders. d Changes in NCX1 expression (red) in bladder c-kit (green)-positive ICC-LCs (white arrows) were detected using IF staining. Bladder ICC-LCs from CYP-8d rats possessed higher NCX1 protein expression than those from naive rats. e , f NCX current (I NCX ) in isolated bladder ICC-LCs from naive and CYP-8d rats was evoked by a step potential ranging from −100 to +60 mV in increments of 10 mV for 200 ms, with a holding potential of −40 mV. The forward and reverse modes of NCX were elicited over a voltage range of −100 to −50 mV and −30 to +60 mV, respectively, and are exhibited as the bottom and upper part of the characteristic I NCX traces. When normalized to cell capacitance, the absolute value of the I NCX density in bladder ICC-LCs from CYP-8d rats significantly exceeded that in bladder ICC-LCs from naive rats over a voltage range of −100 to −80 mV (forward mode) and +10 to +60 mV (reverse mode). Notably, the increase in the reverse mode of NCX was more significant (the data represent the mean ± S.D., n = 8; * P

    Article Snippet: After being blocked with 5% skim milk dissolved in Tris-buffered saline at room temperature for 2 h, the membranes were incubated overnight at 4 °C with various primary antibodies targeting the following proteins: IL-6 (Abcam, Cambridge, UK, ab9324, 1:800), TNF-α (Abcam, ab199013, 1:500), Piezo1 (Alomone labs, Jerusalem, Israel, APC-087, 1:500), NCX1 (Abcam, ab2869, 1:800), NCX2 (Alomone labs, ANX-012, 1:500), NCX3 (Alomone labs, ANX-013, 1:500), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Beyotime, AG019, 1:1000), and α-tubulin (Beyotime, AT819, 1:1000).

    Techniques: Expressing, Immunocytochemistry, Staining, Isolation, Mass Spectrometry

    Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Journal:

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    doi: 10.1091/mbc.E05-11-1027

    Figure Lengend Snippet: Parkin suppresses PICK1-mediated potentiation of ASIC2a currents. (A–E) Representative ASIC2a inward currents (1 st response and 11 th response) evoked by consecutive application of extracellular solution at pH 5.0, 2 min apart, before and after

    Article Snippet: The antibodies used were: mouse monoclonal Flag and synaptophysin (Sigma-Aldrich), PSD95, NR1, CASK and synuclein (BD Bioscience, San Jose, CA), ubiquitin (Covance, Madison, WI), 9E10 myc, C2 actin, and PRK8 parkin (Santa Cruz Biotechnology, Santa Cruz, CA); rabbit polyclonal MALS-1/Veli-1 (Zymed, San Francisco, CA), ASIC2a (Alomone Laboratories, Jerusalem, Israel), PICK1 (kind gift from Dr. Richard Huganir, Johns Hopkins, Baltimore, MD), PICK1 (Affinity BioReagents, Golden, CO), and parkin (Cell Signaling, Beverly, MA); goat polyclonal N-18 PICK1 (Santa Cruz).

    Techniques:

    Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Journal:

    Article Title: Parkin-mediated Monoubiquitination of the PDZ Protein PICK1 Regulates the Activity of Acid-sensing Ion Channels

    doi: 10.1091/mbc.E05-11-1027

    Figure Lengend Snippet: Parkin does not affect ASIC2a levels or native ASIC current densities. (A and B) Steady state endogenous ASIC2a levels in mouse whole brain lysates (A) and cortical neurons (B) from parkin wild-type and knockout mice. Equal amounts of protein were loaded

    Article Snippet: The antibodies used were: mouse monoclonal Flag and synaptophysin (Sigma-Aldrich), PSD95, NR1, CASK and synuclein (BD Bioscience, San Jose, CA), ubiquitin (Covance, Madison, WI), 9E10 myc, C2 actin, and PRK8 parkin (Santa Cruz Biotechnology, Santa Cruz, CA); rabbit polyclonal MALS-1/Veli-1 (Zymed, San Francisco, CA), ASIC2a (Alomone Laboratories, Jerusalem, Israel), PICK1 (kind gift from Dr. Richard Huganir, Johns Hopkins, Baltimore, MD), PICK1 (Affinity BioReagents, Golden, CO), and parkin (Cell Signaling, Beverly, MA); goat polyclonal N-18 PICK1 (Santa Cruz).

    Techniques: Knock-Out, Mouse Assay