rabbit anti vgat  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti vgat
    Schematic representation of 3’ UTR of <t>KCC2</t> and <t>VGAT</t> mRNA with complementary binding sequences for miR-92b-3p and Luciferase Assay validation. (A) 3’UTR of KCC2 (Slc12a5 gene) mRNA demonstrates four complimentary binding sequences (response elements) for the seed region of miR-92b-3p. (B) 3’UTR of VGAT (Slc32a1 gene) mRNA carries one complimentary binding site for miR-92b-3p. (C) miR-92b-3p interaction with 3’UTRs of KCC2 and VGAT in HEK293 cells. The percentage reduction in luciferase activity of the cells transfected with plasmid carrying either KCC2 or VGAT 3’UTRs and miR-92b-3p was calculated by considering 100% luciferase activity for cells transfected only with plasmid carrying the 3’UTR of the target gene. The data represent the normalized luciferase activity of three different sets of transfections. The percentage reduction in luciferase reduction activity of cells transfected with KCC2 3’UTR and miR 92b-3p mimic is significantly higher than cells transfected with KCC2 and miR-control mimic (fig. 4C, ** p
    Rabbit Anti Vgat, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti vgat/product/Alomone Labs
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit anti vgat - by Bioz Stars, 2022-08
    90/100 stars

    Images

    1) Product Images from "miRNA-mediated downregulation of KCC2 and VGAT expression in spinal cord contributes to neonatal cystitis-induced visceral pain in rats"

    Article Title: miRNA-mediated downregulation of KCC2 and VGAT expression in spinal cord contributes to neonatal cystitis-induced visceral pain in rats

    Journal: Pain

    doi: 10.1097/j.pain.0000000000001057

    Schematic representation of 3’ UTR of KCC2 and VGAT mRNA with complementary binding sequences for miR-92b-3p and Luciferase Assay validation. (A) 3’UTR of KCC2 (Slc12a5 gene) mRNA demonstrates four complimentary binding sequences (response elements) for the seed region of miR-92b-3p. (B) 3’UTR of VGAT (Slc32a1 gene) mRNA carries one complimentary binding site for miR-92b-3p. (C) miR-92b-3p interaction with 3’UTRs of KCC2 and VGAT in HEK293 cells. The percentage reduction in luciferase activity of the cells transfected with plasmid carrying either KCC2 or VGAT 3’UTRs and miR-92b-3p was calculated by considering 100% luciferase activity for cells transfected only with plasmid carrying the 3’UTR of the target gene. The data represent the normalized luciferase activity of three different sets of transfections. The percentage reduction in luciferase reduction activity of cells transfected with KCC2 3’UTR and miR 92b-3p mimic is significantly higher than cells transfected with KCC2 and miR-control mimic (fig. 4C, ** p
    Figure Legend Snippet: Schematic representation of 3’ UTR of KCC2 and VGAT mRNA with complementary binding sequences for miR-92b-3p and Luciferase Assay validation. (A) 3’UTR of KCC2 (Slc12a5 gene) mRNA demonstrates four complimentary binding sequences (response elements) for the seed region of miR-92b-3p. (B) 3’UTR of VGAT (Slc32a1 gene) mRNA carries one complimentary binding site for miR-92b-3p. (C) miR-92b-3p interaction with 3’UTRs of KCC2 and VGAT in HEK293 cells. The percentage reduction in luciferase activity of the cells transfected with plasmid carrying either KCC2 or VGAT 3’UTRs and miR-92b-3p was calculated by considering 100% luciferase activity for cells transfected only with plasmid carrying the 3’UTR of the target gene. The data represent the normalized luciferase activity of three different sets of transfections. The percentage reduction in luciferase reduction activity of cells transfected with KCC2 3’UTR and miR 92b-3p mimic is significantly higher than cells transfected with KCC2 and miR-control mimic (fig. 4C, ** p

    Techniques Used: Binding Assay, Luciferase, Activity Assay, Transfection, Plasmid Preparation

    Dual in situ hybridization and immunohistochemistry in spinal cord (L6-S1) demonstrate co-expression of miR-92b-3p with both presynaptic and postsynaptic GABA-associated transporters, cotransporter, receptor in spinal dorsal horn neurons. The close arrows demonstrate co-expression of miR-92b-3p (green) with KCC2 in fig. 6D and with GABA Aα-1 in fig. 6H (red). The VGAT staining that are not colocalized but closely apposed to miR-92b-3p expression are shown in fig. 6L (open arrows). The scale bars for A, E I are 50 µm, for B, C, D, F, G H are 10 µm, for J, K L are 5 µm.
    Figure Legend Snippet: Dual in situ hybridization and immunohistochemistry in spinal cord (L6-S1) demonstrate co-expression of miR-92b-3p with both presynaptic and postsynaptic GABA-associated transporters, cotransporter, receptor in spinal dorsal horn neurons. The close arrows demonstrate co-expression of miR-92b-3p (green) with KCC2 in fig. 6D and with GABA Aα-1 in fig. 6H (red). The VGAT staining that are not colocalized but closely apposed to miR-92b-3p expression are shown in fig. 6L (open arrows). The scale bars for A, E I are 50 µm, for B, C, D, F, G H are 10 µm, for J, K L are 5 µm.

    Techniques Used: In Situ Hybridization, Immunohistochemistry, Expressing, Staining

    2) Product Images from "miRNA-mediated downregulation of KCC2 and VGAT expression in spinal cord contributes to neonatal cystitis-induced visceral pain in rats"

    Article Title: miRNA-mediated downregulation of KCC2 and VGAT expression in spinal cord contributes to neonatal cystitis-induced visceral pain in rats

    Journal: Pain

    doi: 10.1097/j.pain.0000000000001057

    Schematic representation of 3’ UTR of KCC2 and VGAT mRNA with complementary binding sequences for miR-92b-3p and Luciferase Assay validation. (A) 3’UTR of KCC2 (Slc12a5 gene) mRNA demonstrates four complimentary binding sequences (response elements) for the seed region of miR-92b-3p. (B) 3’UTR of VGAT (Slc32a1 gene) mRNA carries one complimentary binding site for miR-92b-3p. (C) miR-92b-3p interaction with 3’UTRs of KCC2 and VGAT in HEK293 cells. The percentage reduction in luciferase activity of the cells transfected with plasmid carrying either KCC2 or VGAT 3’UTRs and miR-92b-3p was calculated by considering 100% luciferase activity for cells transfected only with plasmid carrying the 3’UTR of the target gene. The data represent the normalized luciferase activity of three different sets of transfections. The percentage reduction in luciferase reduction activity of cells transfected with KCC2 3’UTR and miR 92b-3p mimic is significantly higher than cells transfected with KCC2 and miR-control mimic (fig. 4C, ** p
    Figure Legend Snippet: Schematic representation of 3’ UTR of KCC2 and VGAT mRNA with complementary binding sequences for miR-92b-3p and Luciferase Assay validation. (A) 3’UTR of KCC2 (Slc12a5 gene) mRNA demonstrates four complimentary binding sequences (response elements) for the seed region of miR-92b-3p. (B) 3’UTR of VGAT (Slc32a1 gene) mRNA carries one complimentary binding site for miR-92b-3p. (C) miR-92b-3p interaction with 3’UTRs of KCC2 and VGAT in HEK293 cells. The percentage reduction in luciferase activity of the cells transfected with plasmid carrying either KCC2 or VGAT 3’UTRs and miR-92b-3p was calculated by considering 100% luciferase activity for cells transfected only with plasmid carrying the 3’UTR of the target gene. The data represent the normalized luciferase activity of three different sets of transfections. The percentage reduction in luciferase reduction activity of cells transfected with KCC2 3’UTR and miR 92b-3p mimic is significantly higher than cells transfected with KCC2 and miR-control mimic (fig. 4C, ** p

    Techniques Used: Binding Assay, Luciferase, Activity Assay, Transfection, Plasmid Preparation

    Dual in situ hybridization and immunohistochemistry in spinal cord (L6-S1) demonstrate co-expression of miR-92b-3p with both presynaptic and postsynaptic GABA-associated transporters, cotransporter, receptor in spinal dorsal horn neurons. The close arrows demonstrate co-expression of miR-92b-3p (green) with KCC2 in fig. 6D and with GABA Aα-1 in fig. 6H (red). The VGAT staining that are not colocalized but closely apposed to miR-92b-3p expression are shown in fig. 6L (open arrows). The scale bars for A, E I are 50 µm, for B, C, D, F, G H are 10 µm, for J, K L are 5 µm.
    Figure Legend Snippet: Dual in situ hybridization and immunohistochemistry in spinal cord (L6-S1) demonstrate co-expression of miR-92b-3p with both presynaptic and postsynaptic GABA-associated transporters, cotransporter, receptor in spinal dorsal horn neurons. The close arrows demonstrate co-expression of miR-92b-3p (green) with KCC2 in fig. 6D and with GABA Aα-1 in fig. 6H (red). The VGAT staining that are not colocalized but closely apposed to miR-92b-3p expression are shown in fig. 6L (open arrows). The scale bars for A, E I are 50 µm, for B, C, D, F, G H are 10 µm, for J, K L are 5 µm.

    Techniques Used: In Situ Hybridization, Immunohistochemistry, Expressing, Staining

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    Alomone Labs rabbit anti vgat
    Schematic representation of 3’ UTR of <t>KCC2</t> and <t>VGAT</t> mRNA with complementary binding sequences for miR-92b-3p and Luciferase Assay validation. (A) 3’UTR of KCC2 (Slc12a5 gene) mRNA demonstrates four complimentary binding sequences (response elements) for the seed region of miR-92b-3p. (B) 3’UTR of VGAT (Slc32a1 gene) mRNA carries one complimentary binding site for miR-92b-3p. (C) miR-92b-3p interaction with 3’UTRs of KCC2 and VGAT in HEK293 cells. The percentage reduction in luciferase activity of the cells transfected with plasmid carrying either KCC2 or VGAT 3’UTRs and miR-92b-3p was calculated by considering 100% luciferase activity for cells transfected only with plasmid carrying the 3’UTR of the target gene. The data represent the normalized luciferase activity of three different sets of transfections. The percentage reduction in luciferase reduction activity of cells transfected with KCC2 3’UTR and miR 92b-3p mimic is significantly higher than cells transfected with KCC2 and miR-control mimic (fig. 4C, ** p
    Rabbit Anti Vgat, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti vgat/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti vgat - by Bioz Stars, 2022-08
    90/100 stars
      Buy from Supplier

    90
    Alomone Labs guinea pig anti vgat
    Schematic representation of 3’ UTR of <t>KCC2</t> and <t>VGAT</t> mRNA with complementary binding sequences for miR-92b-3p and Luciferase Assay validation. (A) 3’UTR of KCC2 (Slc12a5 gene) mRNA demonstrates four complimentary binding sequences (response elements) for the seed region of miR-92b-3p. (B) 3’UTR of VGAT (Slc32a1 gene) mRNA carries one complimentary binding site for miR-92b-3p. (C) miR-92b-3p interaction with 3’UTRs of KCC2 and VGAT in HEK293 cells. The percentage reduction in luciferase activity of the cells transfected with plasmid carrying either KCC2 or VGAT 3’UTRs and miR-92b-3p was calculated by considering 100% luciferase activity for cells transfected only with plasmid carrying the 3’UTR of the target gene. The data represent the normalized luciferase activity of three different sets of transfections. The percentage reduction in luciferase reduction activity of cells transfected with KCC2 3’UTR and miR 92b-3p mimic is significantly higher than cells transfected with KCC2 and miR-control mimic (fig. 4C, ** p
    Guinea Pig Anti Vgat, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti vgat/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti vgat - by Bioz Stars, 2022-08
    90/100 stars
      Buy from Supplier

    Image Search Results


    Schematic representation of 3’ UTR of KCC2 and VGAT mRNA with complementary binding sequences for miR-92b-3p and Luciferase Assay validation. (A) 3’UTR of KCC2 (Slc12a5 gene) mRNA demonstrates four complimentary binding sequences (response elements) for the seed region of miR-92b-3p. (B) 3’UTR of VGAT (Slc32a1 gene) mRNA carries one complimentary binding site for miR-92b-3p. (C) miR-92b-3p interaction with 3’UTRs of KCC2 and VGAT in HEK293 cells. The percentage reduction in luciferase activity of the cells transfected with plasmid carrying either KCC2 or VGAT 3’UTRs and miR-92b-3p was calculated by considering 100% luciferase activity for cells transfected only with plasmid carrying the 3’UTR of the target gene. The data represent the normalized luciferase activity of three different sets of transfections. The percentage reduction in luciferase reduction activity of cells transfected with KCC2 3’UTR and miR 92b-3p mimic is significantly higher than cells transfected with KCC2 and miR-control mimic (fig. 4C, ** p

    Journal: Pain

    Article Title: miRNA-mediated downregulation of KCC2 and VGAT expression in spinal cord contributes to neonatal cystitis-induced visceral pain in rats

    doi: 10.1097/j.pain.0000000000001057

    Figure Lengend Snippet: Schematic representation of 3’ UTR of KCC2 and VGAT mRNA with complementary binding sequences for miR-92b-3p and Luciferase Assay validation. (A) 3’UTR of KCC2 (Slc12a5 gene) mRNA demonstrates four complimentary binding sequences (response elements) for the seed region of miR-92b-3p. (B) 3’UTR of VGAT (Slc32a1 gene) mRNA carries one complimentary binding site for miR-92b-3p. (C) miR-92b-3p interaction with 3’UTRs of KCC2 and VGAT in HEK293 cells. The percentage reduction in luciferase activity of the cells transfected with plasmid carrying either KCC2 or VGAT 3’UTRs and miR-92b-3p was calculated by considering 100% luciferase activity for cells transfected only with plasmid carrying the 3’UTR of the target gene. The data represent the normalized luciferase activity of three different sets of transfections. The percentage reduction in luciferase reduction activity of cells transfected with KCC2 3’UTR and miR 92b-3p mimic is significantly higher than cells transfected with KCC2 and miR-control mimic (fig. 4C, ** p

    Article Snippet: After completing the treatment with anti-digoxigenin-fluorescence (1:500, Roche) for 2hr at room temperature, spinal cord tissue sections were incubated overnight with either one of the following antibodies at 1:100 dilution, rabbit anti-KCC2 (Millipore), rabbit anti-VGAT (Alomone Labs) and rabbit anti-GABAA-α1 (Alomone Labs).

    Techniques: Binding Assay, Luciferase, Activity Assay, Transfection, Plasmid Preparation

    Dual in situ hybridization and immunohistochemistry in spinal cord (L6-S1) demonstrate co-expression of miR-92b-3p with both presynaptic and postsynaptic GABA-associated transporters, cotransporter, receptor in spinal dorsal horn neurons. The close arrows demonstrate co-expression of miR-92b-3p (green) with KCC2 in fig. 6D and with GABA Aα-1 in fig. 6H (red). The VGAT staining that are not colocalized but closely apposed to miR-92b-3p expression are shown in fig. 6L (open arrows). The scale bars for A, E I are 50 µm, for B, C, D, F, G H are 10 µm, for J, K L are 5 µm.

    Journal: Pain

    Article Title: miRNA-mediated downregulation of KCC2 and VGAT expression in spinal cord contributes to neonatal cystitis-induced visceral pain in rats

    doi: 10.1097/j.pain.0000000000001057

    Figure Lengend Snippet: Dual in situ hybridization and immunohistochemistry in spinal cord (L6-S1) demonstrate co-expression of miR-92b-3p with both presynaptic and postsynaptic GABA-associated transporters, cotransporter, receptor in spinal dorsal horn neurons. The close arrows demonstrate co-expression of miR-92b-3p (green) with KCC2 in fig. 6D and with GABA Aα-1 in fig. 6H (red). The VGAT staining that are not colocalized but closely apposed to miR-92b-3p expression are shown in fig. 6L (open arrows). The scale bars for A, E I are 50 µm, for B, C, D, F, G H are 10 µm, for J, K L are 5 µm.

    Article Snippet: After completing the treatment with anti-digoxigenin-fluorescence (1:500, Roche) for 2hr at room temperature, spinal cord tissue sections were incubated overnight with either one of the following antibodies at 1:100 dilution, rabbit anti-KCC2 (Millipore), rabbit anti-VGAT (Alomone Labs) and rabbit anti-GABAA-α1 (Alomone Labs).

    Techniques: In Situ Hybridization, Immunohistochemistry, Expressing, Staining