rabbit anti gabaα1 antibodies  (Alomone Labs)


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    Alomone Labs rabbit anti gabaα1 antibodies
    Defective Glut3 trafficking in the brain of adult HD 140Q/140Q mice. A) Antibody binding to detect cell surface Glut3 and <t>GABAα1</t> in non-permeabilized brain sections of 9–10 month old WT and HD mice. To ascertain that anti-Glut3 and anti-GABAα1 antibodies labeled only cell surface molecules, we omitted detergents in all buffer solutions used for this assay to preserve the intactness of the plasma membrane. Antibodies bound to cell surface Glut3 and GABAα1 were detected using the immunoperoxidase method. Shown in A are images of Glut3 labeling obtained with a SPOT camera. Three WT and 3 age- and gender- matched HD mice were used for analysis. B) and C) Bar graphs show results of densitometry of Glut3 labeled neurons in brain slices for Glut3 surface expression and neuronal cross-sectional area in the cortex and the striatum of adult WT and HD mice. The data were obtained from digital images of 12 microscopic fields of 6 WT and 6 HD brain sections from 2 WT and 2 HD mice, which were treated under exactly the same conditions. The arbitrary units (A.U.) measured with NIH ImageJ were used for calculating Mean ± SD signal intensity per cell. Statistical significance was determined by two-tailed Student’s t -test using N = 12 microscopic fields. D) Antibody binding to detect cell surface GABAα1 in non-permeabilized brain sections. Brain sections were from the same mice as used for detecting Glut3 expression in A) . Shown are images obtained with a SPOT camera. E) and F) Bar graphs show results for cross sectional areas and signal intensities of GABAα1 positive neurons in the cortex A . Digital images were taken from 7 microscopic fields from 4 WT and 4 HD brain sections from 2 WT and 2 HD mice in (E) and for GABAα1 signal intensities in (F) . Two sections of each animal were used for analysis. For each section, one or two microscopic fields were selected. Error bars in both E and F represent standard deviations. Two-tailed Student’s t -test was performed to determine the statistical significance using N = 7 microscopic fields.
    Rabbit Anti Gabaα1 Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gabaα1 antibodies/product/Alomone Labs
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gabaα1 antibodies - by Bioz Stars, 2022-12
    85/100 stars

    Images

    1) Product Images from "Glucose transporter 3 is a rab11-dependent trafficking cargo and its transport to the cell surface is reduced in neurons of CAG140 Huntington’s disease mice"

    Article Title: Glucose transporter 3 is a rab11-dependent trafficking cargo and its transport to the cell surface is reduced in neurons of CAG140 Huntington’s disease mice

    Journal: Acta Neuropathologica Communications

    doi: 10.1186/s40478-014-0178-7

    Defective Glut3 trafficking in the brain of adult HD 140Q/140Q mice. A) Antibody binding to detect cell surface Glut3 and GABAα1 in non-permeabilized brain sections of 9–10 month old WT and HD mice. To ascertain that anti-Glut3 and anti-GABAα1 antibodies labeled only cell surface molecules, we omitted detergents in all buffer solutions used for this assay to preserve the intactness of the plasma membrane. Antibodies bound to cell surface Glut3 and GABAα1 were detected using the immunoperoxidase method. Shown in A are images of Glut3 labeling obtained with a SPOT camera. Three WT and 3 age- and gender- matched HD mice were used for analysis. B) and C) Bar graphs show results of densitometry of Glut3 labeled neurons in brain slices for Glut3 surface expression and neuronal cross-sectional area in the cortex and the striatum of adult WT and HD mice. The data were obtained from digital images of 12 microscopic fields of 6 WT and 6 HD brain sections from 2 WT and 2 HD mice, which were treated under exactly the same conditions. The arbitrary units (A.U.) measured with NIH ImageJ were used for calculating Mean ± SD signal intensity per cell. Statistical significance was determined by two-tailed Student’s t -test using N = 12 microscopic fields. D) Antibody binding to detect cell surface GABAα1 in non-permeabilized brain sections. Brain sections were from the same mice as used for detecting Glut3 expression in A) . Shown are images obtained with a SPOT camera. E) and F) Bar graphs show results for cross sectional areas and signal intensities of GABAα1 positive neurons in the cortex A . Digital images were taken from 7 microscopic fields from 4 WT and 4 HD brain sections from 2 WT and 2 HD mice in (E) and for GABAα1 signal intensities in (F) . Two sections of each animal were used for analysis. For each section, one or two microscopic fields were selected. Error bars in both E and F represent standard deviations. Two-tailed Student’s t -test was performed to determine the statistical significance using N = 7 microscopic fields.
    Figure Legend Snippet: Defective Glut3 trafficking in the brain of adult HD 140Q/140Q mice. A) Antibody binding to detect cell surface Glut3 and GABAα1 in non-permeabilized brain sections of 9–10 month old WT and HD mice. To ascertain that anti-Glut3 and anti-GABAα1 antibodies labeled only cell surface molecules, we omitted detergents in all buffer solutions used for this assay to preserve the intactness of the plasma membrane. Antibodies bound to cell surface Glut3 and GABAα1 were detected using the immunoperoxidase method. Shown in A are images of Glut3 labeling obtained with a SPOT camera. Three WT and 3 age- and gender- matched HD mice were used for analysis. B) and C) Bar graphs show results of densitometry of Glut3 labeled neurons in brain slices for Glut3 surface expression and neuronal cross-sectional area in the cortex and the striatum of adult WT and HD mice. The data were obtained from digital images of 12 microscopic fields of 6 WT and 6 HD brain sections from 2 WT and 2 HD mice, which were treated under exactly the same conditions. The arbitrary units (A.U.) measured with NIH ImageJ were used for calculating Mean ± SD signal intensity per cell. Statistical significance was determined by two-tailed Student’s t -test using N = 12 microscopic fields. D) Antibody binding to detect cell surface GABAα1 in non-permeabilized brain sections. Brain sections were from the same mice as used for detecting Glut3 expression in A) . Shown are images obtained with a SPOT camera. E) and F) Bar graphs show results for cross sectional areas and signal intensities of GABAα1 positive neurons in the cortex A . Digital images were taken from 7 microscopic fields from 4 WT and 4 HD brain sections from 2 WT and 2 HD mice in (E) and for GABAα1 signal intensities in (F) . Two sections of each animal were used for analysis. For each section, one or two microscopic fields were selected. Error bars in both E and F represent standard deviations. Two-tailed Student’s t -test was performed to determine the statistical significance using N = 7 microscopic fields.

    Techniques Used: Mouse Assay, Binding Assay, Labeling, Expressing, Two Tailed Test

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    Alomone Labs rabbit anti gabaα1 antibodies
    Defective Glut3 trafficking in the brain of adult HD 140Q/140Q mice. A) Antibody binding to detect cell surface Glut3 and <t>GABAα1</t> in non-permeabilized brain sections of 9–10 month old WT and HD mice. To ascertain that anti-Glut3 and anti-GABAα1 antibodies labeled only cell surface molecules, we omitted detergents in all buffer solutions used for this assay to preserve the intactness of the plasma membrane. Antibodies bound to cell surface Glut3 and GABAα1 were detected using the immunoperoxidase method. Shown in A are images of Glut3 labeling obtained with a SPOT camera. Three WT and 3 age- and gender- matched HD mice were used for analysis. B) and C) Bar graphs show results of densitometry of Glut3 labeled neurons in brain slices for Glut3 surface expression and neuronal cross-sectional area in the cortex and the striatum of adult WT and HD mice. The data were obtained from digital images of 12 microscopic fields of 6 WT and 6 HD brain sections from 2 WT and 2 HD mice, which were treated under exactly the same conditions. The arbitrary units (A.U.) measured with NIH ImageJ were used for calculating Mean ± SD signal intensity per cell. Statistical significance was determined by two-tailed Student’s t -test using N = 12 microscopic fields. D) Antibody binding to detect cell surface GABAα1 in non-permeabilized brain sections. Brain sections were from the same mice as used for detecting Glut3 expression in A) . Shown are images obtained with a SPOT camera. E) and F) Bar graphs show results for cross sectional areas and signal intensities of GABAα1 positive neurons in the cortex A . Digital images were taken from 7 microscopic fields from 4 WT and 4 HD brain sections from 2 WT and 2 HD mice in (E) and for GABAα1 signal intensities in (F) . Two sections of each animal were used for analysis. For each section, one or two microscopic fields were selected. Error bars in both E and F represent standard deviations. Two-tailed Student’s t -test was performed to determine the statistical significance using N = 7 microscopic fields.
    Rabbit Anti Gabaα1 Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gabaα1 antibodies/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gabaα1 antibodies - by Bioz Stars, 2022-12
    85/100 stars
      Buy from Supplier

    90
    Alomone Labs ansp 2
    Defective Glut3 trafficking in the brain of adult HD 140Q/140Q mice. A) Antibody binding to detect cell surface Glut3 and <t>GABAα1</t> in non-permeabilized brain sections of 9–10 month old WT and HD mice. To ascertain that anti-Glut3 and anti-GABAα1 antibodies labeled only cell surface molecules, we omitted detergents in all buffer solutions used for this assay to preserve the intactness of the plasma membrane. Antibodies bound to cell surface Glut3 and GABAα1 were detected using the immunoperoxidase method. Shown in A are images of Glut3 labeling obtained with a SPOT camera. Three WT and 3 age- and gender- matched HD mice were used for analysis. B) and C) Bar graphs show results of densitometry of Glut3 labeled neurons in brain slices for Glut3 surface expression and neuronal cross-sectional area in the cortex and the striatum of adult WT and HD mice. The data were obtained from digital images of 12 microscopic fields of 6 WT and 6 HD brain sections from 2 WT and 2 HD mice, which were treated under exactly the same conditions. The arbitrary units (A.U.) measured with NIH ImageJ were used for calculating Mean ± SD signal intensity per cell. Statistical significance was determined by two-tailed Student’s t -test using N = 12 microscopic fields. D) Antibody binding to detect cell surface GABAα1 in non-permeabilized brain sections. Brain sections were from the same mice as used for detecting Glut3 expression in A) . Shown are images obtained with a SPOT camera. E) and F) Bar graphs show results for cross sectional areas and signal intensities of GABAα1 positive neurons in the cortex A . Digital images were taken from 7 microscopic fields from 4 WT and 4 HD brain sections from 2 WT and 2 HD mice in (E) and for GABAα1 signal intensities in (F) . Two sections of each animal were used for analysis. For each section, one or two microscopic fields were selected. Error bars in both E and F represent standard deviations. Two-tailed Student’s t -test was performed to determine the statistical significance using N = 7 microscopic fields.
    Ansp 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ansp 2/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ansp 2 - by Bioz Stars, 2022-12
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    Defective Glut3 trafficking in the brain of adult HD 140Q/140Q mice. A) Antibody binding to detect cell surface Glut3 and GABAα1 in non-permeabilized brain sections of 9–10 month old WT and HD mice. To ascertain that anti-Glut3 and anti-GABAα1 antibodies labeled only cell surface molecules, we omitted detergents in all buffer solutions used for this assay to preserve the intactness of the plasma membrane. Antibodies bound to cell surface Glut3 and GABAα1 were detected using the immunoperoxidase method. Shown in A are images of Glut3 labeling obtained with a SPOT camera. Three WT and 3 age- and gender- matched HD mice were used for analysis. B) and C) Bar graphs show results of densitometry of Glut3 labeled neurons in brain slices for Glut3 surface expression and neuronal cross-sectional area in the cortex and the striatum of adult WT and HD mice. The data were obtained from digital images of 12 microscopic fields of 6 WT and 6 HD brain sections from 2 WT and 2 HD mice, which were treated under exactly the same conditions. The arbitrary units (A.U.) measured with NIH ImageJ were used for calculating Mean ± SD signal intensity per cell. Statistical significance was determined by two-tailed Student’s t -test using N = 12 microscopic fields. D) Antibody binding to detect cell surface GABAα1 in non-permeabilized brain sections. Brain sections were from the same mice as used for detecting Glut3 expression in A) . Shown are images obtained with a SPOT camera. E) and F) Bar graphs show results for cross sectional areas and signal intensities of GABAα1 positive neurons in the cortex A . Digital images were taken from 7 microscopic fields from 4 WT and 4 HD brain sections from 2 WT and 2 HD mice in (E) and for GABAα1 signal intensities in (F) . Two sections of each animal were used for analysis. For each section, one or two microscopic fields were selected. Error bars in both E and F represent standard deviations. Two-tailed Student’s t -test was performed to determine the statistical significance using N = 7 microscopic fields.

    Journal: Acta Neuropathologica Communications

    Article Title: Glucose transporter 3 is a rab11-dependent trafficking cargo and its transport to the cell surface is reduced in neurons of CAG140 Huntington’s disease mice

    doi: 10.1186/s40478-014-0178-7

    Figure Lengend Snippet: Defective Glut3 trafficking in the brain of adult HD 140Q/140Q mice. A) Antibody binding to detect cell surface Glut3 and GABAα1 in non-permeabilized brain sections of 9–10 month old WT and HD mice. To ascertain that anti-Glut3 and anti-GABAα1 antibodies labeled only cell surface molecules, we omitted detergents in all buffer solutions used for this assay to preserve the intactness of the plasma membrane. Antibodies bound to cell surface Glut3 and GABAα1 were detected using the immunoperoxidase method. Shown in A are images of Glut3 labeling obtained with a SPOT camera. Three WT and 3 age- and gender- matched HD mice were used for analysis. B) and C) Bar graphs show results of densitometry of Glut3 labeled neurons in brain slices for Glut3 surface expression and neuronal cross-sectional area in the cortex and the striatum of adult WT and HD mice. The data were obtained from digital images of 12 microscopic fields of 6 WT and 6 HD brain sections from 2 WT and 2 HD mice, which were treated under exactly the same conditions. The arbitrary units (A.U.) measured with NIH ImageJ were used for calculating Mean ± SD signal intensity per cell. Statistical significance was determined by two-tailed Student’s t -test using N = 12 microscopic fields. D) Antibody binding to detect cell surface GABAα1 in non-permeabilized brain sections. Brain sections were from the same mice as used for detecting Glut3 expression in A) . Shown are images obtained with a SPOT camera. E) and F) Bar graphs show results for cross sectional areas and signal intensities of GABAα1 positive neurons in the cortex A . Digital images were taken from 7 microscopic fields from 4 WT and 4 HD brain sections from 2 WT and 2 HD mice in (E) and for GABAα1 signal intensities in (F) . Two sections of each animal were used for analysis. For each section, one or two microscopic fields were selected. Error bars in both E and F represent standard deviations. Two-tailed Student’s t -test was performed to determine the statistical significance using N = 7 microscopic fields.

    Article Snippet: Brain sections were blocked with 5% donkey serum, 1% bovine serum albumin, and 0.03% hydrogen peroxide in PBS and incubated with goat anti-Glut3 antibodies (I-14, Santa Cruz Biotechnology) or rabbit anti-GABAα1 antibodies (Alomone Labs) at 4°C for two days followed by incubation with biotinylated anti-goat or anti-rabbit IgG(H + L) at 4°C overnight.

    Techniques: Mouse Assay, Binding Assay, Labeling, Expressing, Two Tailed Test