orai2  (Alomone Labs)


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    Name:
    Anti Orai2 Antibody
    Description:
    Anti Orai2 Antibody ACC 061 is a highly specific antibody directed against an epitope of the mouse protein The antibody can be used in western blot and immunohistochemistry applications It has been designed to recognize Orai2 from human mouse and rat samples
    Catalog Number:
    ACC-061
    Price:
    495.0
    Category:
    Primary Antibody
    Applications:
    Immunofluorescence, Immunohistochemistry, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    50 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs orai2
    Anti Orai2 Antibody
    Anti Orai2 Antibody ACC 061 is a highly specific antibody directed against an epitope of the mouse protein The antibody can be used in western blot and immunohistochemistry applications It has been designed to recognize Orai2 from human mouse and rat samples
    https://www.bioz.com/result/orai2/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    orai2 - by Bioz Stars, 2021-10
    93/100 stars

    Images

    1) Product Images from "Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes"

    Article Title: Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0208981

    Correlation between the mRNA expression in PBMCs and demographic characteristics of type 1 diabetic donors. A. Correlation between ORAI2 and age at onset of type 1 diabetes. B. Correlation between Ca V 2.1 and duration of Type 1 diabetes. The correlation was accessed using non-parametric Spearman rank test. * indicates p value
    Figure Legend Snippet: Correlation between the mRNA expression in PBMCs and demographic characteristics of type 1 diabetic donors. A. Correlation between ORAI2 and age at onset of type 1 diabetes. B. Correlation between Ca V 2.1 and duration of Type 1 diabetes. The correlation was accessed using non-parametric Spearman rank test. * indicates p value

    Techniques Used: Expressing

    Altered mRNA expression of specific calcium release activated calcium (CRACs) channel and voltage-gated calcium channel (Ca v ) subunits in PBMCs from non-pregnant controls and pregnant women. Data from each group is presented as scatter dot plot (°) or box and whiskers plot with median and whiskers plotted by Tukey method to determine outliers (• - above or below the whiskers). Ca V 1.1 subunit mRNA was not detected in any sample. Statistical analysis was performed by excluding outliers depending on normality distribution of the data and only the subunits with statistically significant differences are mentioned below. One-Way ANOVA with Bonferroni post-hoc test: ORAI1, df = 48, p = 0.003; Kruskal–Wallis ANOVA on ranks with Dunn’s post hoc test: ORAI2, H (1, 46) = 28.5, p
    Figure Legend Snippet: Altered mRNA expression of specific calcium release activated calcium (CRACs) channel and voltage-gated calcium channel (Ca v ) subunits in PBMCs from non-pregnant controls and pregnant women. Data from each group is presented as scatter dot plot (°) or box and whiskers plot with median and whiskers plotted by Tukey method to determine outliers (• - above or below the whiskers). Ca V 1.1 subunit mRNA was not detected in any sample. Statistical analysis was performed by excluding outliers depending on normality distribution of the data and only the subunits with statistically significant differences are mentioned below. One-Way ANOVA with Bonferroni post-hoc test: ORAI1, df = 48, p = 0.003; Kruskal–Wallis ANOVA on ranks with Dunn’s post hoc test: ORAI2, H (1, 46) = 28.5, p

    Techniques Used: Expressing

    2) Product Images from "Inhibition of Polyamine Biosynthesis Reverses Ca2+ Channel Remodeling in Colon Cancer Cells"

    Article Title: Inhibition of Polyamine Biosynthesis Reverses Ca2+ Channel Remodeling in Colon Cancer Cells

    Journal: Cancers

    doi: 10.3390/cancers11010083

    Effects of DFMO on the expression of proteins involved in SOCE in colon cancer HT29 cells. HT29 cells were treated with vehicle (control) or DFMO 5 mM, and then cells were lysed and subjected to Western blotting with antibodies against TRPC1, STIM1, STIM2, ORAI1, ORAI2 and ORAI3, followed by reprobing with anti-β-actin antibody for protein loading control. Bar graphs represent specific protein expression normalized to the β-actin content. Data are from n = 3 experiments (* p
    Figure Legend Snippet: Effects of DFMO on the expression of proteins involved in SOCE in colon cancer HT29 cells. HT29 cells were treated with vehicle (control) or DFMO 5 mM, and then cells were lysed and subjected to Western blotting with antibodies against TRPC1, STIM1, STIM2, ORAI1, ORAI2 and ORAI3, followed by reprobing with anti-β-actin antibody for protein loading control. Bar graphs represent specific protein expression normalized to the β-actin content. Data are from n = 3 experiments (* p

    Techniques Used: Expressing, Western Blot

    Effects of DFMO on the expression of genes coding for SOCE molecular players in HT29 cells. mRNA expression levels of selected genes were determined using qRT-PCR of extracts from control and DFMO-treated HT29 cells. β-actin was used as a reference. Data results are mean ± SEM from DFMO-treated cells relative to untreated cells Data are from n = 7, 7, 6, 6, 6, and 5 experiments for TRPC1 , STIM1 , STIM2 , ORAI1 , ORAI2 , and ORAI3 , respectively * p
    Figure Legend Snippet: Effects of DFMO on the expression of genes coding for SOCE molecular players in HT29 cells. mRNA expression levels of selected genes were determined using qRT-PCR of extracts from control and DFMO-treated HT29 cells. β-actin was used as a reference. Data results are mean ± SEM from DFMO-treated cells relative to untreated cells Data are from n = 7, 7, 6, 6, 6, and 5 experiments for TRPC1 , STIM1 , STIM2 , ORAI1 , ORAI2 , and ORAI3 , respectively * p

    Techniques Used: Expressing, Quantitative RT-PCR

    3) Product Images from "A Reciprocal Shift in Transient Receptor Potential Channel 1 (TRPC1) and Stromal Interaction Molecule 2 (STIM2) Contributes to Ca2+ Remodeling and Cancer Hallmarks in Colorectal Carcinoma Cells"

    Article Title: A Reciprocal Shift in Transient Receptor Potential Channel 1 (TRPC1) and Stromal Interaction Molecule 2 (STIM2) Contributes to Ca2+ Remodeling and Cancer Hallmarks in Colorectal Carcinoma Cells

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M114.581678

    Effects of ORAI2 and ORAI3 knockdown on SOCE in colon carcinoma (HT29) cells. A, HT29 cells were transfected with scramble siRNA or siRNA for ORAI2 or ORAI3 , and levels of corresponding mRNAs were estimated by quantitative RT-PCR. B, SOCE was estimated
    Figure Legend Snippet: Effects of ORAI2 and ORAI3 knockdown on SOCE in colon carcinoma (HT29) cells. A, HT29 cells were transfected with scramble siRNA or siRNA for ORAI2 or ORAI3 , and levels of corresponding mRNAs were estimated by quantitative RT-PCR. B, SOCE was estimated

    Techniques Used: Transfection, Quantitative RT-PCR

    4) Product Images from "Orai/CRACM1 and KCa3.1 ion channels interact in the human lung mast cell plasma membrane"

    Article Title: Orai/CRACM1 and KCa3.1 ion channels interact in the human lung mast cell plasma membrane

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-015-0112-z

    Orai2 and K Ca 3.1 proteins do not co-immunoprecipitate under the conditions used to co-immunoprecipitate Orai1 and K Ca 3.1. a Western blots using either an antibody recognising the myc epitope (left) or an antibody recognising the FLAG epitope (right) of HEK293 cell lysates. Lysates expressed either myc epitope tagged Orai2, FLAG epitope-tagged K Ca 3.1, or both as indicated in the panel above. b HEK293 cell lysates expressing the proteins indicated in the panel above were immunoprecipitated with an anti-myc antibody. Immunoprecipitates were then Western blotted using either an anti-Orai2 antibody (left) or an anti-FLAG antibody (right). Control HEK293 cell lysate expressing K Ca 3.1-FLAG protein. c As ( b ) except cell lysates were immunoprecipitated with an anti-FLAG antibody and then Western blotted with an anti-Orai2 antibody (left) or an anti-FLAG antibody (right). Control HEK293 cell lysate expressing Orai2-myc protein. Blots shown are representative of 3 independent experiments
    Figure Legend Snippet: Orai2 and K Ca 3.1 proteins do not co-immunoprecipitate under the conditions used to co-immunoprecipitate Orai1 and K Ca 3.1. a Western blots using either an antibody recognising the myc epitope (left) or an antibody recognising the FLAG epitope (right) of HEK293 cell lysates. Lysates expressed either myc epitope tagged Orai2, FLAG epitope-tagged K Ca 3.1, or both as indicated in the panel above. b HEK293 cell lysates expressing the proteins indicated in the panel above were immunoprecipitated with an anti-myc antibody. Immunoprecipitates were then Western blotted using either an anti-Orai2 antibody (left) or an anti-FLAG antibody (right). Control HEK293 cell lysate expressing K Ca 3.1-FLAG protein. c As ( b ) except cell lysates were immunoprecipitated with an anti-FLAG antibody and then Western blotted with an anti-Orai2 antibody (left) or an anti-FLAG antibody (right). Control HEK293 cell lysate expressing Orai2-myc protein. Blots shown are representative of 3 independent experiments

    Techniques Used: Western Blot, FLAG-tag, Expressing, Immunoprecipitation

    Orai1 and K Ca 3.1 co-localise in the plasma membrane. a HEK293 cells, dually transfected with FLAG-tagged K Ca 3.1 and myc-tagged Orai1 and then immunostained, show co-localisation in the plasma membrane by single plane confocal microscopy (top panels). Dually transfected HEK293 show negative staining for appropriate isotype controls (bottom panels): rabbit IgG control, dual stained with anti-myc, and mouse IgG1 control dual stained with anti-FLAG. b Fluorescence intensity plot shows increased fluorescence at the plasma membrane. myc-Orai1 is shown in green and FLAG-K Ca 3.1 in red. Arrows indicate increased fluorescence where the region of interest (ROI) intersects the plasma membrane. c HEK293 cells, dually transfected with FLAG-tagged K Ca 3.1 and myc-tagged Orai2 and then immunostained, show poor co-localisation in the plasma membrane by single plane confocal microscopy (top panels). Dually transfected HEK293 show negative staining for appropriate isotype controls (bottom panels): rabbit IgG control, dual stained with anti-myc, and mouse IgG1 control dual stained with anti-FLAG. d Fluorescence intensity plot shows poor co-localisation of K Ca 3.1 and Orai2 signals. myc-Orai2 is shown in green and FLAG-K Ca 3.1 in red. Scale bars are 10 μm
    Figure Legend Snippet: Orai1 and K Ca 3.1 co-localise in the plasma membrane. a HEK293 cells, dually transfected with FLAG-tagged K Ca 3.1 and myc-tagged Orai1 and then immunostained, show co-localisation in the plasma membrane by single plane confocal microscopy (top panels). Dually transfected HEK293 show negative staining for appropriate isotype controls (bottom panels): rabbit IgG control, dual stained with anti-myc, and mouse IgG1 control dual stained with anti-FLAG. b Fluorescence intensity plot shows increased fluorescence at the plasma membrane. myc-Orai1 is shown in green and FLAG-K Ca 3.1 in red. Arrows indicate increased fluorescence where the region of interest (ROI) intersects the plasma membrane. c HEK293 cells, dually transfected with FLAG-tagged K Ca 3.1 and myc-tagged Orai2 and then immunostained, show poor co-localisation in the plasma membrane by single plane confocal microscopy (top panels). Dually transfected HEK293 show negative staining for appropriate isotype controls (bottom panels): rabbit IgG control, dual stained with anti-myc, and mouse IgG1 control dual stained with anti-FLAG. d Fluorescence intensity plot shows poor co-localisation of K Ca 3.1 and Orai2 signals. myc-Orai2 is shown in green and FLAG-K Ca 3.1 in red. Scale bars are 10 μm

    Techniques Used: Transfection, Confocal Microscopy, Negative Staining, Staining, Fluorescence

    Related Articles

    Incubation:

    Article Title: ORAI channels are critical for receptor-mediated endocytosis of albumin
    Article Snippet: .. The blot was then incubated with polyclonal anti-ORAI1 (ACC-060), anti-ORAI2 (ACC-061) or anti-STIM1 (ACC-063) at 1:500 dilution (Alomone Labs, Jerusalem, Israel), and anti-ORAI3 (#4117) at 1:500 dilution (ProSci Incorporated, Poway, CA, USA) overnight at 4 °C, respectively, and washed with phosphate buffered saline (PBS) and then incubated with a goat anti-rabbit IgG-HRP (1:2000 dilution) (A0545, Sigma). ..

    Article Title: Hypoxia selectively upregulates cation channels and increases cytosolic [Ca2+] in pulmonary, but not coronary, arterial smooth muscle cells
    Article Snippet: .. After blocking with 5% skim milk in Tris-buffered saline supplemented with 0.1% Tween 20 for 1 h at room temperature, the membranes were incubated at 4°C overnight with primary antibody against STIM1 (diluted 1:1,000; catalog no. 4119, Prosci, Poway, CA) , STIM2 (diluted 1:1,000; catalog no. S8572, Sigma, St. Louis, MO) , Orai1 (diluted 1:1,000; catalog no. ACC-060, Alomone, Jerusalem, Israel) ( , ), Orai2 (diluted 1:1,000; catalog no. ACC-061, Alomone) , and TRPC6 (diluted 1:1,000; catalog no. ACC-120, Alomone) ( ). ..

    Article Title: Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes
    Article Snippet: .. The membranes were blocked with 10% FBS in Tris buffered saline containing 0.1% Tween (TBS-T) for 1 h and incubated overnight at 4°C with primary antibodies against STIM2 (1:200, Cell Signaling Technology, Cat No. 4917), ORAI1 (1:500, Alomone labs, Cat No. ACC-060), ORAI2 (1:500, Alomone labs, Cat No. ACC-061), CaV 1.3 (1:500, Alomone labs, Cat No. ACC-005), CaV 2.3 (1:500, Alomone labs, Cat No. ACC-006) and GAPDH (1:3000; Merck Millipore, Cat No. ABS16). ..

    Article Title: Orai1-Orai2 complex is involved in store-operated calcium entry in chondrocyte cell lines.
    Article Snippet: .. Ca(2+) influx via store-operated Ca(2+) entry (SOCE) plays critical roles in many essential cellular functions.. The Ca(2+) release-activated Ca(2+) (CRAC) channel complex, consisting of Orai and STIM, is one of the major components of store-operated Ca(2+) (SOC) channels. ..

    Blocking Assay:

    Article Title: Hypoxia selectively upregulates cation channels and increases cytosolic [Ca2+] in pulmonary, but not coronary, arterial smooth muscle cells
    Article Snippet: .. After blocking with 5% skim milk in Tris-buffered saline supplemented with 0.1% Tween 20 for 1 h at room temperature, the membranes were incubated at 4°C overnight with primary antibody against STIM1 (diluted 1:1,000; catalog no. 4119, Prosci, Poway, CA) , STIM2 (diluted 1:1,000; catalog no. S8572, Sigma, St. Louis, MO) , Orai1 (diluted 1:1,000; catalog no. ACC-060, Alomone, Jerusalem, Israel) ( , ), Orai2 (diluted 1:1,000; catalog no. ACC-061, Alomone) , and TRPC6 (diluted 1:1,000; catalog no. ACC-120, Alomone) ( ). ..

    other:

    Article Title: Inhibition of Polyamine Biosynthesis Reverses Ca2+ Channel Remodeling in Colon Cancer Cells
    Article Snippet: Antibodies against TRPC1, Orai1, Orai2, and STIM1 were from Alomone Labs (Jerusalem, Israel).

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  • 93
    Alomone Labs orai2
    Correlation between the mRNA expression in PBMCs and demographic characteristics of type 1 diabetic donors. A. Correlation between <t>ORAI2</t> and age at onset of type 1 diabetes. B. Correlation between Ca V 2.1 and duration of Type 1 diabetes. The correlation was accessed using non-parametric Spearman rank test. * indicates p value
    Orai2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orai2/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    orai2 - by Bioz Stars, 2021-10
    93/100 stars
      Buy from Supplier

    90
    Alomone Labs kcnmb4
    BK Ca openers control spasticity. (A) RNAseq expression of BK Ca channel components in EA.hy926 cells. Inset represents the western blot of <t>KCNMB4</t> in EA.hy926 cells, which was tested in four samples, and western blot of KCNMB4 expression in the spinal cord in control ( n = 3) and spastic EAE spinal cord tissues (two shown, n = 6 total) showing an additional prominent band, not present in EA.hy926 cells, above the anticipated fragment size more notable during EAE. This antibody detected multiple bands in western blot analyses. (B) RNAseq expression of BK Ca channel components in the spinal cords of spastic EAE ( n = 3 mice) and age‐matched normal mice ( n = 3 mice). The results represent the mean ± SD. Inset represents the western blot of KCNMA1 in control and spastic EAE tissue (Antibody APC‐021 detected a single band at the anticipated size and was repeated with antibody APC‐151 with comparable results), and insets show a significant change in ratio between Kcnma1 and neurofilament light ( Nefl ) gene levels. (C) Percentage change in resistance to flexion of spastic hindlimbs in ABH mice in response to treatment with 5 mg·kg −1 p.o. VSN16R in water ( n = 10 animals, n = 19 limbs) that were pretreated with saline or 1 mg·kg −1 i.p. paxilline ( n = 7 animals, n = 14 limbs). (D) Percentage change in resistance to flexion of hindlimbs in ABH mice following injection i.p. with 1 mg·kg −1 paxilline ( n = 7 animals, n = 13 limbs), 40 mg·kg −1 i.p. BMS‐204352 ( n = 7 animals, n = 13 limbs), 20 mg·kg −1 i.p. NS‐1619 ( n = 7 animals, n = 14 limbs) or 10 mg·kg −1 i.p. NS‐11021 ( n = 7 animals, n = 13 limbs). *Significant difference compared with baseline.
    Kcnmb4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kcnmb4/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kcnmb4 - by Bioz Stars, 2021-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    Correlation between the mRNA expression in PBMCs and demographic characteristics of type 1 diabetic donors. A. Correlation between ORAI2 and age at onset of type 1 diabetes. B. Correlation between Ca V 2.1 and duration of Type 1 diabetes. The correlation was accessed using non-parametric Spearman rank test. * indicates p value

    Journal: PLoS ONE

    Article Title: Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes

    doi: 10.1371/journal.pone.0208981

    Figure Lengend Snippet: Correlation between the mRNA expression in PBMCs and demographic characteristics of type 1 diabetic donors. A. Correlation between ORAI2 and age at onset of type 1 diabetes. B. Correlation between Ca V 2.1 and duration of Type 1 diabetes. The correlation was accessed using non-parametric Spearman rank test. * indicates p value

    Article Snippet: The membranes were blocked with 10% FBS in Tris buffered saline containing 0.1% Tween (TBS-T) for 1 h and incubated overnight at 4°C with primary antibodies against STIM2 (1:200, Cell Signaling Technology, Cat No. 4917), ORAI1 (1:500, Alomone labs, Cat No. ACC-060), ORAI2 (1:500, Alomone labs, Cat No. ACC-061), CaV 1.3 (1:500, Alomone labs, Cat No. ACC-005), CaV 2.3 (1:500, Alomone labs, Cat No. ACC-006) and GAPDH (1:3000; Merck Millipore, Cat No. ABS16).

    Techniques: Expressing

    Altered mRNA expression of specific calcium release activated calcium (CRACs) channel and voltage-gated calcium channel (Ca v ) subunits in PBMCs from non-pregnant controls and pregnant women. Data from each group is presented as scatter dot plot (°) or box and whiskers plot with median and whiskers plotted by Tukey method to determine outliers (• - above or below the whiskers). Ca V 1.1 subunit mRNA was not detected in any sample. Statistical analysis was performed by excluding outliers depending on normality distribution of the data and only the subunits with statistically significant differences are mentioned below. One-Way ANOVA with Bonferroni post-hoc test: ORAI1, df = 48, p = 0.003; Kruskal–Wallis ANOVA on ranks with Dunn’s post hoc test: ORAI2, H (1, 46) = 28.5, p

    Journal: PLoS ONE

    Article Title: Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes

    doi: 10.1371/journal.pone.0208981

    Figure Lengend Snippet: Altered mRNA expression of specific calcium release activated calcium (CRACs) channel and voltage-gated calcium channel (Ca v ) subunits in PBMCs from non-pregnant controls and pregnant women. Data from each group is presented as scatter dot plot (°) or box and whiskers plot with median and whiskers plotted by Tukey method to determine outliers (• - above or below the whiskers). Ca V 1.1 subunit mRNA was not detected in any sample. Statistical analysis was performed by excluding outliers depending on normality distribution of the data and only the subunits with statistically significant differences are mentioned below. One-Way ANOVA with Bonferroni post-hoc test: ORAI1, df = 48, p = 0.003; Kruskal–Wallis ANOVA on ranks with Dunn’s post hoc test: ORAI2, H (1, 46) = 28.5, p

    Article Snippet: The membranes were blocked with 10% FBS in Tris buffered saline containing 0.1% Tween (TBS-T) for 1 h and incubated overnight at 4°C with primary antibodies against STIM2 (1:200, Cell Signaling Technology, Cat No. 4917), ORAI1 (1:500, Alomone labs, Cat No. ACC-060), ORAI2 (1:500, Alomone labs, Cat No. ACC-061), CaV 1.3 (1:500, Alomone labs, Cat No. ACC-005), CaV 2.3 (1:500, Alomone labs, Cat No. ACC-006) and GAPDH (1:3000; Merck Millipore, Cat No. ABS16).

    Techniques: Expressing

    BK Ca openers control spasticity. (A) RNAseq expression of BK Ca channel components in EA.hy926 cells. Inset represents the western blot of KCNMB4 in EA.hy926 cells, which was tested in four samples, and western blot of KCNMB4 expression in the spinal cord in control ( n = 3) and spastic EAE spinal cord tissues (two shown, n = 6 total) showing an additional prominent band, not present in EA.hy926 cells, above the anticipated fragment size more notable during EAE. This antibody detected multiple bands in western blot analyses. (B) RNAseq expression of BK Ca channel components in the spinal cords of spastic EAE ( n = 3 mice) and age‐matched normal mice ( n = 3 mice). The results represent the mean ± SD. Inset represents the western blot of KCNMA1 in control and spastic EAE tissue (Antibody APC‐021 detected a single band at the anticipated size and was repeated with antibody APC‐151 with comparable results), and insets show a significant change in ratio between Kcnma1 and neurofilament light ( Nefl ) gene levels. (C) Percentage change in resistance to flexion of spastic hindlimbs in ABH mice in response to treatment with 5 mg·kg −1 p.o. VSN16R in water ( n = 10 animals, n = 19 limbs) that were pretreated with saline or 1 mg·kg −1 i.p. paxilline ( n = 7 animals, n = 14 limbs). (D) Percentage change in resistance to flexion of hindlimbs in ABH mice following injection i.p. with 1 mg·kg −1 paxilline ( n = 7 animals, n = 13 limbs), 40 mg·kg −1 i.p. BMS‐204352 ( n = 7 animals, n = 13 limbs), 20 mg·kg −1 i.p. NS‐1619 ( n = 7 animals, n = 14 limbs) or 10 mg·kg −1 i.p. NS‐11021 ( n = 7 animals, n = 13 limbs). *Significant difference compared with baseline.

    Journal: British Journal of Pharmacology

    Article Title: Big conductance calcium‐activated potassium channel openers control spasticity without sedation) Big conductance calcium‐activated potassium channel openers control spasticity without sedation

    doi: 10.1111/bph.13889

    Figure Lengend Snippet: BK Ca openers control spasticity. (A) RNAseq expression of BK Ca channel components in EA.hy926 cells. Inset represents the western blot of KCNMB4 in EA.hy926 cells, which was tested in four samples, and western blot of KCNMB4 expression in the spinal cord in control ( n = 3) and spastic EAE spinal cord tissues (two shown, n = 6 total) showing an additional prominent band, not present in EA.hy926 cells, above the anticipated fragment size more notable during EAE. This antibody detected multiple bands in western blot analyses. (B) RNAseq expression of BK Ca channel components in the spinal cords of spastic EAE ( n = 3 mice) and age‐matched normal mice ( n = 3 mice). The results represent the mean ± SD. Inset represents the western blot of KCNMA1 in control and spastic EAE tissue (Antibody APC‐021 detected a single band at the anticipated size and was repeated with antibody APC‐151 with comparable results), and insets show a significant change in ratio between Kcnma1 and neurofilament light ( Nefl ) gene levels. (C) Percentage change in resistance to flexion of spastic hindlimbs in ABH mice in response to treatment with 5 mg·kg −1 p.o. VSN16R in water ( n = 10 animals, n = 19 limbs) that were pretreated with saline or 1 mg·kg −1 i.p. paxilline ( n = 7 animals, n = 14 limbs). (D) Percentage change in resistance to flexion of hindlimbs in ABH mice following injection i.p. with 1 mg·kg −1 paxilline ( n = 7 animals, n = 13 limbs), 40 mg·kg −1 i.p. BMS‐204352 ( n = 7 animals, n = 13 limbs), 20 mg·kg −1 i.p. NS‐1619 ( n = 7 animals, n = 14 limbs) or 10 mg·kg −1 i.p. NS‐11021 ( n = 7 animals, n = 13 limbs). *Significant difference compared with baseline.

    Article Snippet: Membranes were washed, blocked for 1 h in blocking buffer (5% non‐fat dried milk) and incubated in blocking buffer with primary antibody using rabbit polyclonal antibodies against mouse/human KCNMA1 (APC‐021, previously validated by lack of activity in big conductance calcium‐activated potassium channel Kcnma1 ‐deficient mice and APC‐151 antibody) and KCNMB4 (APC‐061 antibody, previously validated by lack of activity in Kcnmb4‐deficient mice despite detecting multiple isoforms), which were purchased from Alomone Labs, Jerusalem Israel, whose website reports supporting literature concerning characterization of the antibodies.

    Techniques: Expressing, Western Blot, Mouse Assay, Injection