orai1  (Alomone Labs)

Journal: PLoS ONE

Article Title: Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes

doi: 10.1371/journal.pone.0208981

Figure Lengend Snippet: Altered mRNA expression of specific calcium release activated calcium (CRACs) channel and voltage-gated calcium channel (Ca v ) subunits in PBMCs from non-diabetic controls and T1D patients. Data from each group is presented as scatter dot plot (°) or box and whiskers plot with median and whiskers plotted by Tukey method to determine outliers (• - above or below the whiskers). Statistical analysis was performed by excluding outliers depending on normality distribution of the data and only the subunits with statistically significant differences are mentioned below. One-Way ANOVA with Bonferroni post-hoc test: STIM2, df = 45, p = 0.002; Kruskal–Wallis ANOVA on ranks with Dunn’s post hoc test: ORAI1, H (1, 47) = 9.1, p

Article Snippet: The membranes were blocked with 10% FBS in Tris buffered saline containing 0.1% Tween (TBS-T) for 1 h and incubated overnight at 4°C with primary antibodies against STIM2 (1:200, Cell Signaling Technology, Cat No. 4917), ORAI1 (1:500, Alomone labs, Cat No. ACC-060), ORAI2 (1:500, Alomone labs, Cat No. ACC-061), CaV 1.3 (1:500, Alomone labs, Cat No. ACC-005), CaV 2.3 (1:500, Alomone labs, Cat No. ACC-006) and GAPDH (1:3000; Merck Millipore, Cat No. ABS16).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Expression of calcium release-activated and voltage-gated calcium channels genes in peripheral blood mononuclear cells is altered in pregnancy and in type 1 diabetes

doi: 10.1371/journal.pone.0208981

Figure Lengend Snippet: Western blot analysis. Western blot analysis showing the expression of Ca 2+ sensor protein stromal interaction molecules (STIM) 2, voltage-gated Ca 2+ (Ca V ) channel subunits Cav1.3 and Cav2.3, and Ca 2+ release-activated Ca 2+ (CRAC) channel proteins ORAI1 and 2 in PBMCs from ND (n = 3 or 4) and T1D (n = 4) individuals. GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) served as loading controls.

Article Snippet: The membranes were blocked with 10% FBS in Tris buffered saline containing 0.1% Tween (TBS-T) for 1 h and incubated overnight at 4°C with primary antibodies against STIM2 (1:200, Cell Signaling Technology, Cat No. 4917), ORAI1 (1:500, Alomone labs, Cat No. ACC-060), ORAI2 (1:500, Alomone labs, Cat No. ACC-061), CaV 1.3 (1:500, Alomone labs, Cat No. ACC-005), CaV 2.3 (1:500, Alomone labs, Cat No. ACC-006) and GAPDH (1:3000; Merck Millipore, Cat No. ABS16).

Techniques: Western Blot, Expressing