polyclonal rabbit anti trek 2 antibody  (Alomone Labs)


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    Alomone Labs polyclonal rabbit anti trek 2 antibody
    <t>TREK-2</t> channels are preferentially expressed on IB4-positive small-sized maxillary TG neurons. (a, b) Fluorescent images show immunostaining for TREK-2 channels (a) and IB4-FITC staining (b) in the same maxillary region of a TG section. Scale bar: 50 µm. (c) Image in the box in A at expanded scale. (d) Image in the box in B at expanded scale. (e) Overlay image of (c) and (d). Scale bar: 20 µm. (f) and (g) Histogram of cell size distribution of TREK-2 immnoreactivity positive (f, n = 304) and IB4 positive (g, n = 466) maxillary TG neurons. (h) Percent of TREK-2-ir positive neurons (red bar, n = 11 TG sections) and IB4 positive neurons (green bar, n = 11 TG sectons) in total maxillary TG neurons. (i) Percent of neurons co-localized with TREK-2-ir and IB4 staining in all TREK-2-ir positive neurons (left bar, n = 11 TG sections) or in all IB4-positive neurons (right bar, n = 11 TG sections). Data represent Mean ± SEM.
    Polyclonal Rabbit Anti Trek 2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti trek 2 antibody/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti trek 2 antibody - by Bioz Stars, 2023-06
    92/100 stars

    Images

    1) Product Images from "Effects of GABA B receptor activation on excitability of IB4-positive maxillary trigeminal ganglion neurons: Possible involvement of TREK2 activation"

    Article Title: Effects of GABA B receptor activation on excitability of IB4-positive maxillary trigeminal ganglion neurons: Possible involvement of TREK2 activation

    Journal: Molecular Pain

    doi: 10.1177/17448069211042963

    TREK-2 channels are preferentially expressed on IB4-positive small-sized maxillary TG neurons. (a, b) Fluorescent images show immunostaining for TREK-2 channels (a) and IB4-FITC staining (b) in the same maxillary region of a TG section. Scale bar: 50 µm. (c) Image in the box in A at expanded scale. (d) Image in the box in B at expanded scale. (e) Overlay image of (c) and (d). Scale bar: 20 µm. (f) and (g) Histogram of cell size distribution of TREK-2 immnoreactivity positive (f, n = 304) and IB4 positive (g, n = 466) maxillary TG neurons. (h) Percent of TREK-2-ir positive neurons (red bar, n = 11 TG sections) and IB4 positive neurons (green bar, n = 11 TG sectons) in total maxillary TG neurons. (i) Percent of neurons co-localized with TREK-2-ir and IB4 staining in all TREK-2-ir positive neurons (left bar, n = 11 TG sections) or in all IB4-positive neurons (right bar, n = 11 TG sections). Data represent Mean ± SEM.
    Figure Legend Snippet: TREK-2 channels are preferentially expressed on IB4-positive small-sized maxillary TG neurons. (a, b) Fluorescent images show immunostaining for TREK-2 channels (a) and IB4-FITC staining (b) in the same maxillary region of a TG section. Scale bar: 50 µm. (c) Image in the box in A at expanded scale. (d) Image in the box in B at expanded scale. (e) Overlay image of (c) and (d). Scale bar: 20 µm. (f) and (g) Histogram of cell size distribution of TREK-2 immnoreactivity positive (f, n = 304) and IB4 positive (g, n = 466) maxillary TG neurons. (h) Percent of TREK-2-ir positive neurons (red bar, n = 11 TG sections) and IB4 positive neurons (green bar, n = 11 TG sectons) in total maxillary TG neurons. (i) Percent of neurons co-localized with TREK-2-ir and IB4 staining in all TREK-2-ir positive neurons (left bar, n = 11 TG sections) or in all IB4-positive neurons (right bar, n = 11 TG sections). Data represent Mean ± SEM.

    Techniques Used: Immunostaining, Staining

    trek 2  (Alomone Labs)


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    Structured Review

    Alomone Labs trek 2
    a Mean mRNA levels of multiple K channels in conventionally grown Capan-1 cell cultures (light gray bars and symbols) and Capan-1 epithelial monolayers (dark gray bars and black symbols) ( n = 4 for each condition). Aliases: KCNF1 (Kv5.1), KCNJ10 (Kir4.1), KCNJ2 (Kir2.1), KCNK1 (TWIK-1), KCNK10 <t>(TREK-2),</t> KCNK15 (TASK-5), KCNK16 (TALK-1), KCNK17 (TALK-2), KCNK2 (TREK-1), KCNK3 (TASK-1), KCNK5 (TASK-2), KCNK6 (TWIK-2), KCNK9 (TASK-3), KCNMA1 (KCa1.1), KCNN3 (KCa2.3), KCNN4 (KCa3.1), and KCNQ1 (Kv7.1). b RT-PCR analysis of KCNE1 mRNA in cell lysate from Capan-1 (Cap.) (H. refers to HPDE, another cell line). M. refers to the marker and Ctrl. to the control, with template substituted by H 2 O. c Western blots of Capan-1 protein samples showing expression of K v 7.1 (KCNQ1), TASK-2 (KCNK5), and TREK-2 (KCNK10). (Western blot for TREK-1 has been shown previously in Capan-1 cells .) * P < 0.05; ** P < 0.01; *** P < 0.001
    Trek 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trek 2 - by Bioz Stars, 2023-06
    86/100 stars

    Images

    1) Product Images from "Synergistic effects of agonists and two-pore-domain potassium channels on secretory responses of human pancreatic duct cells Capan-1"

    Article Title: Synergistic effects of agonists and two-pore-domain potassium channels on secretory responses of human pancreatic duct cells Capan-1

    Journal: Pflugers Archiv

    doi: 10.1007/s00424-022-02782-9

    a Mean mRNA levels of multiple K channels in conventionally grown Capan-1 cell cultures (light gray bars and symbols) and Capan-1 epithelial monolayers (dark gray bars and black symbols) ( n = 4 for each condition). Aliases: KCNF1 (Kv5.1), KCNJ10 (Kir4.1), KCNJ2 (Kir2.1), KCNK1 (TWIK-1), KCNK10 (TREK-2), KCNK15 (TASK-5), KCNK16 (TALK-1), KCNK17 (TALK-2), KCNK2 (TREK-1), KCNK3 (TASK-1), KCNK5 (TASK-2), KCNK6 (TWIK-2), KCNK9 (TASK-3), KCNMA1 (KCa1.1), KCNN3 (KCa2.3), KCNN4 (KCa3.1), and KCNQ1 (Kv7.1). b RT-PCR analysis of KCNE1 mRNA in cell lysate from Capan-1 (Cap.) (H. refers to HPDE, another cell line). M. refers to the marker and Ctrl. to the control, with template substituted by H 2 O. c Western blots of Capan-1 protein samples showing expression of K v 7.1 (KCNQ1), TASK-2 (KCNK5), and TREK-2 (KCNK10). (Western blot for TREK-1 has been shown previously in Capan-1 cells .) * P < 0.05; ** P < 0.01; *** P < 0.001
    Figure Legend Snippet: a Mean mRNA levels of multiple K channels in conventionally grown Capan-1 cell cultures (light gray bars and symbols) and Capan-1 epithelial monolayers (dark gray bars and black symbols) ( n = 4 for each condition). Aliases: KCNF1 (Kv5.1), KCNJ10 (Kir4.1), KCNJ2 (Kir2.1), KCNK1 (TWIK-1), KCNK10 (TREK-2), KCNK15 (TASK-5), KCNK16 (TALK-1), KCNK17 (TALK-2), KCNK2 (TREK-1), KCNK3 (TASK-1), KCNK5 (TASK-2), KCNK6 (TWIK-2), KCNK9 (TASK-3), KCNMA1 (KCa1.1), KCNN3 (KCa2.3), KCNN4 (KCa3.1), and KCNQ1 (Kv7.1). b RT-PCR analysis of KCNE1 mRNA in cell lysate from Capan-1 (Cap.) (H. refers to HPDE, another cell line). M. refers to the marker and Ctrl. to the control, with template substituted by H 2 O. c Western blots of Capan-1 protein samples showing expression of K v 7.1 (KCNQ1), TASK-2 (KCNK5), and TREK-2 (KCNK10). (Western blot for TREK-1 has been shown previously in Capan-1 cells .) * P < 0.05; ** P < 0.01; *** P < 0.001

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Marker, Western Blot, Expressing

    Immunolocalization of TREK-1, TREK-2, and TASK2 K2P channels (green color) in Capan-1 monolayers. Nuclei were stained with DAPI; red color indicates actin staining. Images were obtained in x – y and x – z scans. Bar is 10 µm
    Figure Legend Snippet: Immunolocalization of TREK-1, TREK-2, and TASK2 K2P channels (green color) in Capan-1 monolayers. Nuclei were stained with DAPI; red color indicates actin staining. Images were obtained in x – y and x – z scans. Bar is 10 µm

    Techniques Used: Staining

    trek 2  (Alomone Labs)


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    Structured Review

    Alomone Labs trek 2
    a Mean mRNA levels of multiple K channels in conventionally grown Capan-1 cell cultures (light gray bars and symbols) and Capan-1 epithelial monolayers (dark gray bars and black symbols) ( n = 4 for each condition). Aliases: KCNF1 (Kv5.1), KCNJ10 (Kir4.1), KCNJ2 (Kir2.1), KCNK1 (TWIK-1), KCNK10 <t>(TREK-2),</t> KCNK15 (TASK-5), KCNK16 (TALK-1), KCNK17 (TALK-2), KCNK2 (TREK-1), KCNK3 (TASK-1), KCNK5 (TASK-2), KCNK6 (TWIK-2), KCNK9 (TASK-3), KCNMA1 (KCa1.1), KCNN3 (KCa2.3), KCNN4 (KCa3.1), and KCNQ1 (Kv7.1). b RT-PCR analysis of KCNE1 mRNA in cell lysate from Capan-1 (Cap.) (H. refers to HPDE, another cell line). M. refers to the marker and Ctrl. to the control, with template substituted by H 2 O. c Western blots of Capan-1 protein samples showing expression of K v 7.1 (KCNQ1), TASK-2 (KCNK5), and TREK-2 (KCNK10). (Western blot for TREK-1 has been shown previously in Capan-1 cells .) * P < 0.05; ** P < 0.01; *** P < 0.001
    Trek 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trek 2/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trek 2 - by Bioz Stars, 2023-06
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    Images

    1) Product Images from "Synergistic effects of agonists and two-pore-domain potassium channels on secretory responses of human pancreatic duct cells Capan-1"

    Article Title: Synergistic effects of agonists and two-pore-domain potassium channels on secretory responses of human pancreatic duct cells Capan-1

    Journal: Pflugers Archiv

    doi: 10.1007/s00424-022-02782-9

    a Mean mRNA levels of multiple K channels in conventionally grown Capan-1 cell cultures (light gray bars and symbols) and Capan-1 epithelial monolayers (dark gray bars and black symbols) ( n = 4 for each condition). Aliases: KCNF1 (Kv5.1), KCNJ10 (Kir4.1), KCNJ2 (Kir2.1), KCNK1 (TWIK-1), KCNK10 (TREK-2), KCNK15 (TASK-5), KCNK16 (TALK-1), KCNK17 (TALK-2), KCNK2 (TREK-1), KCNK3 (TASK-1), KCNK5 (TASK-2), KCNK6 (TWIK-2), KCNK9 (TASK-3), KCNMA1 (KCa1.1), KCNN3 (KCa2.3), KCNN4 (KCa3.1), and KCNQ1 (Kv7.1). b RT-PCR analysis of KCNE1 mRNA in cell lysate from Capan-1 (Cap.) (H. refers to HPDE, another cell line). M. refers to the marker and Ctrl. to the control, with template substituted by H 2 O. c Western blots of Capan-1 protein samples showing expression of K v 7.1 (KCNQ1), TASK-2 (KCNK5), and TREK-2 (KCNK10). (Western blot for TREK-1 has been shown previously in Capan-1 cells .) * P < 0.05; ** P < 0.01; *** P < 0.001
    Figure Legend Snippet: a Mean mRNA levels of multiple K channels in conventionally grown Capan-1 cell cultures (light gray bars and symbols) and Capan-1 epithelial monolayers (dark gray bars and black symbols) ( n = 4 for each condition). Aliases: KCNF1 (Kv5.1), KCNJ10 (Kir4.1), KCNJ2 (Kir2.1), KCNK1 (TWIK-1), KCNK10 (TREK-2), KCNK15 (TASK-5), KCNK16 (TALK-1), KCNK17 (TALK-2), KCNK2 (TREK-1), KCNK3 (TASK-1), KCNK5 (TASK-2), KCNK6 (TWIK-2), KCNK9 (TASK-3), KCNMA1 (KCa1.1), KCNN3 (KCa2.3), KCNN4 (KCa3.1), and KCNQ1 (Kv7.1). b RT-PCR analysis of KCNE1 mRNA in cell lysate from Capan-1 (Cap.) (H. refers to HPDE, another cell line). M. refers to the marker and Ctrl. to the control, with template substituted by H 2 O. c Western blots of Capan-1 protein samples showing expression of K v 7.1 (KCNQ1), TASK-2 (KCNK5), and TREK-2 (KCNK10). (Western blot for TREK-1 has been shown previously in Capan-1 cells .) * P < 0.05; ** P < 0.01; *** P < 0.001

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Marker, Western Blot, Expressing

    Immunolocalization of TREK-1, TREK-2, and TASK2 K2P channels (green color) in Capan-1 monolayers. Nuclei were stained with DAPI; red color indicates actin staining. Images were obtained in x – y and x – z scans. Bar is 10 µm
    Figure Legend Snippet: Immunolocalization of TREK-1, TREK-2, and TASK2 K2P channels (green color) in Capan-1 monolayers. Nuclei were stained with DAPI; red color indicates actin staining. Images were obtained in x – y and x – z scans. Bar is 10 µm

    Techniques Used: Staining

    polyclonal rabbit anti trek 2 antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs polyclonal rabbit anti trek 2 antibody
    <t>TREK-2</t> channels are preferentially expressed on IB4-positive small-sized maxillary TG neurons. (a, b) Fluorescent images show immunostaining for TREK-2 channels (a) and IB4-FITC staining (b) in the same maxillary region of a TG section. Scale bar: 50 µm. (c) Image in the box in A at expanded scale. (d) Image in the box in B at expanded scale. (e) Overlay image of (c) and (d). Scale bar: 20 µm. (f) and (g) Histogram of cell size distribution of TREK-2 immnoreactivity positive (f, n = 304) and IB4 positive (g, n = 466) maxillary TG neurons. (h) Percent of TREK-2-ir positive neurons (red bar, n = 11 TG sections) and IB4 positive neurons (green bar, n = 11 TG sectons) in total maxillary TG neurons. (i) Percent of neurons co-localized with TREK-2-ir and IB4 staining in all TREK-2-ir positive neurons (left bar, n = 11 TG sections) or in all IB4-positive neurons (right bar, n = 11 TG sections). Data represent Mean ± SEM.
    Polyclonal Rabbit Anti Trek 2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti trek 2 antibody/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti trek 2 antibody - by Bioz Stars, 2023-06
    92/100 stars

    Images

    1) Product Images from "Effects of GABA B receptor activation on excitability of IB4-positive maxillary trigeminal ganglion neurons: Possible involvement of TREK2 activation"

    Article Title: Effects of GABA B receptor activation on excitability of IB4-positive maxillary trigeminal ganglion neurons: Possible involvement of TREK2 activation

    Journal: Molecular Pain

    doi: 10.1177/17448069211042963

    TREK-2 channels are preferentially expressed on IB4-positive small-sized maxillary TG neurons. (a, b) Fluorescent images show immunostaining for TREK-2 channels (a) and IB4-FITC staining (b) in the same maxillary region of a TG section. Scale bar: 50 µm. (c) Image in the box in A at expanded scale. (d) Image in the box in B at expanded scale. (e) Overlay image of (c) and (d). Scale bar: 20 µm. (f) and (g) Histogram of cell size distribution of TREK-2 immnoreactivity positive (f, n = 304) and IB4 positive (g, n = 466) maxillary TG neurons. (h) Percent of TREK-2-ir positive neurons (red bar, n = 11 TG sections) and IB4 positive neurons (green bar, n = 11 TG sectons) in total maxillary TG neurons. (i) Percent of neurons co-localized with TREK-2-ir and IB4 staining in all TREK-2-ir positive neurons (left bar, n = 11 TG sections) or in all IB4-positive neurons (right bar, n = 11 TG sections). Data represent Mean ± SEM.
    Figure Legend Snippet: TREK-2 channels are preferentially expressed on IB4-positive small-sized maxillary TG neurons. (a, b) Fluorescent images show immunostaining for TREK-2 channels (a) and IB4-FITC staining (b) in the same maxillary region of a TG section. Scale bar: 50 µm. (c) Image in the box in A at expanded scale. (d) Image in the box in B at expanded scale. (e) Overlay image of (c) and (d). Scale bar: 20 µm. (f) and (g) Histogram of cell size distribution of TREK-2 immnoreactivity positive (f, n = 304) and IB4 positive (g, n = 466) maxillary TG neurons. (h) Percent of TREK-2-ir positive neurons (red bar, n = 11 TG sections) and IB4 positive neurons (green bar, n = 11 TG sectons) in total maxillary TG neurons. (i) Percent of neurons co-localized with TREK-2-ir and IB4 staining in all TREK-2-ir positive neurons (left bar, n = 11 TG sections) or in all IB4-positive neurons (right bar, n = 11 TG sections). Data represent Mean ± SEM.

    Techniques Used: Immunostaining, Staining

    rabbit anti k2p 10 1  (Alomone Labs)


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    Alomone Labs rabbit anti k2p 10 1
    A) Left, TREK-1 immunoreactivity (TREK-1-ir) at a NR and MBP immunoreactivity (MBP-ir) on myelin sheath. Right, TREK-1-ir at a NR and CASPR-ir in paranodal regions. B) Left, TRAAK-ir at a NR and MBP-ir on myelin sheath. Right, TRAAK-ir at a NR and CASPR-ir in paranodal regions. C) Similar to A&B except TREK-2-ir was examined and was negative at NRs. In A-C, NRs are indicated by arrows. MBP, myelin basic protein. CASPR, contactin associated protein. D) Summary of immunoreactive nodes for experiments represented in A-C: 112/129 nodes were TREK-1-ir positive, 118/129 nodes were TRAAK-ir positive. 0/129 nodes were TREK-2-ir positive. E) HEK293 cells transfected with TREK-1/eGFP (left), TRAAK/mCherry (middle), and both TREK-1/EGFP and TRAAK/mCherry (right). F) Traces illustrate single channel currents recorded at −80 mV from an HEK293 cell transfected with TREK-1/eGFP (upper, homomeric TREK-1) or an HEK293 cell transfected with TRAAK/mCherry (lower, homomeric TRAAK). Bottom, I-V curves of single channel currents recorded at different transmembrane voltages for homomeric TREK-1 channels (open circles, n = 7) or homomeric TRAAK (solid circles, n = 6). G) Sample traces show two types of single channels recorded at −80 mV from a HEK293 cell co-transfected with TREK-1/eGFP and TRAAK/mCherry plasmids, one type (upper, TREK-1/TRAAK) has unitary currents apparently larger than homomeric channels and another type (lower, TREK-1-like) has unitary currents similar to homomeric TREK-1 channels shown in F. Bottom panel, I-V curves of the currents of TREK-1/TRAAK single channels (n = 12, solid triangles) and TREK-1-like single channels (n = 13, open triangles). H) Summary of single channel conductance at −80 mV (open bars) and 80 mV (closed bars) for homomeric TREK-1 (n = 5 at −80 mV, n = 6 at 80 mV), homomeric TRAAK (n = 6 at both voltages), TREK-1-like (n = 12 at −80 mV, n = 5 at 80 mV), and TREK-1/TRAAK channels (n = 8 at −80 mV, n = 5 at 80 mV) expressed in HEK293 cells. The single channel conductance of nodal <t>K2P</t> channels (n = 13 at both voltages) is also included in the graph for a comparison. The single channel conductance at −80 mV was used for comparison. All recordings were performed under the cell-attached mode. Data represent Mean ± SEM, ns, no significant difference, *p < 0.05, ***p < 0.001, one-way ANOVA with the Tukey post hoc test. See also Fig. S5–9
    Rabbit Anti K2p 10 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti k2p 10 1/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti k2p 10 1 - by Bioz Stars, 2023-06
    92/100 stars

    Images

    1) Product Images from "TREK-1 and TRAAK are principal K + channels at the nodes of Ranvier for rapid action potential conduction on mammalian myelinated afferent nerves"

    Article Title: TREK-1 and TRAAK are principal K + channels at the nodes of Ranvier for rapid action potential conduction on mammalian myelinated afferent nerves

    Journal: Neuron

    doi: 10.1016/j.neuron.2019.08.042

    A) Left, TREK-1 immunoreactivity (TREK-1-ir) at a NR and MBP immunoreactivity (MBP-ir) on myelin sheath. Right, TREK-1-ir at a NR and CASPR-ir in paranodal regions. B) Left, TRAAK-ir at a NR and MBP-ir on myelin sheath. Right, TRAAK-ir at a NR and CASPR-ir in paranodal regions. C) Similar to A&B except TREK-2-ir was examined and was negative at NRs. In A-C, NRs are indicated by arrows. MBP, myelin basic protein. CASPR, contactin associated protein. D) Summary of immunoreactive nodes for experiments represented in A-C: 112/129 nodes were TREK-1-ir positive, 118/129 nodes were TRAAK-ir positive. 0/129 nodes were TREK-2-ir positive. E) HEK293 cells transfected with TREK-1/eGFP (left), TRAAK/mCherry (middle), and both TREK-1/EGFP and TRAAK/mCherry (right). F) Traces illustrate single channel currents recorded at −80 mV from an HEK293 cell transfected with TREK-1/eGFP (upper, homomeric TREK-1) or an HEK293 cell transfected with TRAAK/mCherry (lower, homomeric TRAAK). Bottom, I-V curves of single channel currents recorded at different transmembrane voltages for homomeric TREK-1 channels (open circles, n = 7) or homomeric TRAAK (solid circles, n = 6). G) Sample traces show two types of single channels recorded at −80 mV from a HEK293 cell co-transfected with TREK-1/eGFP and TRAAK/mCherry plasmids, one type (upper, TREK-1/TRAAK) has unitary currents apparently larger than homomeric channels and another type (lower, TREK-1-like) has unitary currents similar to homomeric TREK-1 channels shown in F. Bottom panel, I-V curves of the currents of TREK-1/TRAAK single channels (n = 12, solid triangles) and TREK-1-like single channels (n = 13, open triangles). H) Summary of single channel conductance at −80 mV (open bars) and 80 mV (closed bars) for homomeric TREK-1 (n = 5 at −80 mV, n = 6 at 80 mV), homomeric TRAAK (n = 6 at both voltages), TREK-1-like (n = 12 at −80 mV, n = 5 at 80 mV), and TREK-1/TRAAK channels (n = 8 at −80 mV, n = 5 at 80 mV) expressed in HEK293 cells. The single channel conductance of nodal K2P channels (n = 13 at both voltages) is also included in the graph for a comparison. The single channel conductance at −80 mV was used for comparison. All recordings were performed under the cell-attached mode. Data represent Mean ± SEM, ns, no significant difference, *p < 0.05, ***p < 0.001, one-way ANOVA with the Tukey post hoc test. See also Fig. S5–9
    Figure Legend Snippet: A) Left, TREK-1 immunoreactivity (TREK-1-ir) at a NR and MBP immunoreactivity (MBP-ir) on myelin sheath. Right, TREK-1-ir at a NR and CASPR-ir in paranodal regions. B) Left, TRAAK-ir at a NR and MBP-ir on myelin sheath. Right, TRAAK-ir at a NR and CASPR-ir in paranodal regions. C) Similar to A&B except TREK-2-ir was examined and was negative at NRs. In A-C, NRs are indicated by arrows. MBP, myelin basic protein. CASPR, contactin associated protein. D) Summary of immunoreactive nodes for experiments represented in A-C: 112/129 nodes were TREK-1-ir positive, 118/129 nodes were TRAAK-ir positive. 0/129 nodes were TREK-2-ir positive. E) HEK293 cells transfected with TREK-1/eGFP (left), TRAAK/mCherry (middle), and both TREK-1/EGFP and TRAAK/mCherry (right). F) Traces illustrate single channel currents recorded at −80 mV from an HEK293 cell transfected with TREK-1/eGFP (upper, homomeric TREK-1) or an HEK293 cell transfected with TRAAK/mCherry (lower, homomeric TRAAK). Bottom, I-V curves of single channel currents recorded at different transmembrane voltages for homomeric TREK-1 channels (open circles, n = 7) or homomeric TRAAK (solid circles, n = 6). G) Sample traces show two types of single channels recorded at −80 mV from a HEK293 cell co-transfected with TREK-1/eGFP and TRAAK/mCherry plasmids, one type (upper, TREK-1/TRAAK) has unitary currents apparently larger than homomeric channels and another type (lower, TREK-1-like) has unitary currents similar to homomeric TREK-1 channels shown in F. Bottom panel, I-V curves of the currents of TREK-1/TRAAK single channels (n = 12, solid triangles) and TREK-1-like single channels (n = 13, open triangles). H) Summary of single channel conductance at −80 mV (open bars) and 80 mV (closed bars) for homomeric TREK-1 (n = 5 at −80 mV, n = 6 at 80 mV), homomeric TRAAK (n = 6 at both voltages), TREK-1-like (n = 12 at −80 mV, n = 5 at 80 mV), and TREK-1/TRAAK channels (n = 8 at −80 mV, n = 5 at 80 mV) expressed in HEK293 cells. The single channel conductance of nodal K2P channels (n = 13 at both voltages) is also included in the graph for a comparison. The single channel conductance at −80 mV was used for comparison. All recordings were performed under the cell-attached mode. Data represent Mean ± SEM, ns, no significant difference, *p < 0.05, ***p < 0.001, one-way ANOVA with the Tukey post hoc test. See also Fig. S5–9

    Techniques Used: Transfection

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, shRNA, Software

    rabbit anti k2p 10 1 trek 2  (Alomone Labs)


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    Alomone Labs rabbit anti k2p 10 1 trek 2
    A) Left, TREK-1 immunoreactivity (TREK-1-ir) at a NR and MBP immunoreactivity (MBP-ir) on myelin sheath. Right, TREK-1-ir at a NR and CASPR-ir in paranodal regions. B) Left, TRAAK-ir at a NR and MBP-ir on myelin sheath. Right, TRAAK-ir at a NR and CASPR-ir in paranodal regions. C) Similar to A&B except <t>TREK-2-ir</t> was examined and was negative at NRs. In A-C, NRs are indicated by arrows. MBP, myelin basic protein. CASPR, contactin associated protein. D) Summary of immunoreactive nodes for experiments represented in A-C: 112/129 nodes were TREK-1-ir positive, 118/129 nodes were TRAAK-ir positive. 0/129 nodes were TREK-2-ir positive. E) HEK293 cells transfected with TREK-1/eGFP (left), TRAAK/mCherry (middle), and both TREK-1/EGFP and TRAAK/mCherry (right). F) Traces illustrate single channel currents recorded at −80 mV from an HEK293 cell transfected with TREK-1/eGFP (upper, homomeric TREK-1) or an HEK293 cell transfected with TRAAK/mCherry (lower, homomeric TRAAK). Bottom, I-V curves of single channel currents recorded at different transmembrane voltages for homomeric TREK-1 channels (open circles, n = 7) or homomeric TRAAK (solid circles, n = 6). G) Sample traces show two types of single channels recorded at −80 mV from a HEK293 cell co-transfected with TREK-1/eGFP and TRAAK/mCherry plasmids, one type (upper, TREK-1/TRAAK) has unitary currents apparently larger than homomeric channels and another type (lower, TREK-1-like) has unitary currents similar to homomeric TREK-1 channels shown in F. Bottom panel, I-V curves of the currents of TREK-1/TRAAK single channels (n = 12, solid triangles) and TREK-1-like single channels (n = 13, open triangles). H) Summary of single channel conductance at −80 mV (open bars) and 80 mV (closed bars) for homomeric TREK-1 (n = 5 at −80 mV, n = 6 at 80 mV), homomeric TRAAK (n = 6 at both voltages), TREK-1-like (n = 12 at −80 mV, n = 5 at 80 mV), and TREK-1/TRAAK channels (n = 8 at −80 mV, n = 5 at 80 mV) expressed in HEK293 cells. The single channel conductance of nodal <t>K2P</t> channels (n = 13 at both voltages) is also included in the graph for a comparison. The single channel conductance at −80 mV was used for comparison. All recordings were performed under the cell-attached mode. Data represent Mean ± SEM, ns, no significant difference, *p < 0.05, ***p < 0.001, one-way ANOVA with the Tukey post hoc test. See also Fig. S5–9
    Rabbit Anti K2p 10 1 Trek 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "TREK-1 and TRAAK are principal K + channels at the nodes of Ranvier for rapid action potential conduction on mammalian myelinated afferent nerves"

    Article Title: TREK-1 and TRAAK are principal K + channels at the nodes of Ranvier for rapid action potential conduction on mammalian myelinated afferent nerves

    Journal: Neuron

    doi: 10.1016/j.neuron.2019.08.042

    A) Left, TREK-1 immunoreactivity (TREK-1-ir) at a NR and MBP immunoreactivity (MBP-ir) on myelin sheath. Right, TREK-1-ir at a NR and CASPR-ir in paranodal regions. B) Left, TRAAK-ir at a NR and MBP-ir on myelin sheath. Right, TRAAK-ir at a NR and CASPR-ir in paranodal regions. C) Similar to A&B except TREK-2-ir was examined and was negative at NRs. In A-C, NRs are indicated by arrows. MBP, myelin basic protein. CASPR, contactin associated protein. D) Summary of immunoreactive nodes for experiments represented in A-C: 112/129 nodes were TREK-1-ir positive, 118/129 nodes were TRAAK-ir positive. 0/129 nodes were TREK-2-ir positive. E) HEK293 cells transfected with TREK-1/eGFP (left), TRAAK/mCherry (middle), and both TREK-1/EGFP and TRAAK/mCherry (right). F) Traces illustrate single channel currents recorded at −80 mV from an HEK293 cell transfected with TREK-1/eGFP (upper, homomeric TREK-1) or an HEK293 cell transfected with TRAAK/mCherry (lower, homomeric TRAAK). Bottom, I-V curves of single channel currents recorded at different transmembrane voltages for homomeric TREK-1 channels (open circles, n = 7) or homomeric TRAAK (solid circles, n = 6). G) Sample traces show two types of single channels recorded at −80 mV from a HEK293 cell co-transfected with TREK-1/eGFP and TRAAK/mCherry plasmids, one type (upper, TREK-1/TRAAK) has unitary currents apparently larger than homomeric channels and another type (lower, TREK-1-like) has unitary currents similar to homomeric TREK-1 channels shown in F. Bottom panel, I-V curves of the currents of TREK-1/TRAAK single channels (n = 12, solid triangles) and TREK-1-like single channels (n = 13, open triangles). H) Summary of single channel conductance at −80 mV (open bars) and 80 mV (closed bars) for homomeric TREK-1 (n = 5 at −80 mV, n = 6 at 80 mV), homomeric TRAAK (n = 6 at both voltages), TREK-1-like (n = 12 at −80 mV, n = 5 at 80 mV), and TREK-1/TRAAK channels (n = 8 at −80 mV, n = 5 at 80 mV) expressed in HEK293 cells. The single channel conductance of nodal K2P channels (n = 13 at both voltages) is also included in the graph for a comparison. The single channel conductance at −80 mV was used for comparison. All recordings were performed under the cell-attached mode. Data represent Mean ± SEM, ns, no significant difference, *p < 0.05, ***p < 0.001, one-way ANOVA with the Tukey post hoc test. See also Fig. S5–9
    Figure Legend Snippet: A) Left, TREK-1 immunoreactivity (TREK-1-ir) at a NR and MBP immunoreactivity (MBP-ir) on myelin sheath. Right, TREK-1-ir at a NR and CASPR-ir in paranodal regions. B) Left, TRAAK-ir at a NR and MBP-ir on myelin sheath. Right, TRAAK-ir at a NR and CASPR-ir in paranodal regions. C) Similar to A&B except TREK-2-ir was examined and was negative at NRs. In A-C, NRs are indicated by arrows. MBP, myelin basic protein. CASPR, contactin associated protein. D) Summary of immunoreactive nodes for experiments represented in A-C: 112/129 nodes were TREK-1-ir positive, 118/129 nodes were TRAAK-ir positive. 0/129 nodes were TREK-2-ir positive. E) HEK293 cells transfected with TREK-1/eGFP (left), TRAAK/mCherry (middle), and both TREK-1/EGFP and TRAAK/mCherry (right). F) Traces illustrate single channel currents recorded at −80 mV from an HEK293 cell transfected with TREK-1/eGFP (upper, homomeric TREK-1) or an HEK293 cell transfected with TRAAK/mCherry (lower, homomeric TRAAK). Bottom, I-V curves of single channel currents recorded at different transmembrane voltages for homomeric TREK-1 channels (open circles, n = 7) or homomeric TRAAK (solid circles, n = 6). G) Sample traces show two types of single channels recorded at −80 mV from a HEK293 cell co-transfected with TREK-1/eGFP and TRAAK/mCherry plasmids, one type (upper, TREK-1/TRAAK) has unitary currents apparently larger than homomeric channels and another type (lower, TREK-1-like) has unitary currents similar to homomeric TREK-1 channels shown in F. Bottom panel, I-V curves of the currents of TREK-1/TRAAK single channels (n = 12, solid triangles) and TREK-1-like single channels (n = 13, open triangles). H) Summary of single channel conductance at −80 mV (open bars) and 80 mV (closed bars) for homomeric TREK-1 (n = 5 at −80 mV, n = 6 at 80 mV), homomeric TRAAK (n = 6 at both voltages), TREK-1-like (n = 12 at −80 mV, n = 5 at 80 mV), and TREK-1/TRAAK channels (n = 8 at −80 mV, n = 5 at 80 mV) expressed in HEK293 cells. The single channel conductance of nodal K2P channels (n = 13 at both voltages) is also included in the graph for a comparison. The single channel conductance at −80 mV was used for comparison. All recordings were performed under the cell-attached mode. Data represent Mean ± SEM, ns, no significant difference, *p < 0.05, ***p < 0.001, one-way ANOVA with the Tukey post hoc test. See also Fig. S5–9

    Techniques Used: Transfection

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, shRNA, Software

    rabbit anti k2p 10 1  (Alomone Labs)


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    Alomone Labs rabbit anti k2p 10 1
    A) Left, TREK-1 immunoreactivity (TREK-1-ir) at a NR and MBP immunoreactivity (MBP-ir) on myelin sheath. Right, TREK-1-ir at a NR and CASPR-ir in paranodal regions. B) Left, TRAAK-ir at a NR and MBP-ir on myelin sheath. Right, TRAAK-ir at a NR and CASPR-ir in paranodal regions. C) Similar to A&B except TREK-2-ir was examined and was negative at NRs. In A-C, NRs are indicated by arrows. MBP, myelin basic protein. CASPR, contactin associated protein. D) Summary of immunoreactive nodes for experiments represented in A-C: 112/129 nodes were TREK-1-ir positive, 118/129 nodes were TRAAK-ir positive. 0/129 nodes were TREK-2-ir positive. E) HEK293 cells transfected with TREK-1/eGFP (left), TRAAK/mCherry (middle), and both TREK-1/EGFP and TRAAK/mCherry (right). F) Traces illustrate single channel currents recorded at −80 mV from an HEK293 cell transfected with TREK-1/eGFP (upper, homomeric TREK-1) or an HEK293 cell transfected with TRAAK/mCherry (lower, homomeric TRAAK). Bottom, I-V curves of single channel currents recorded at different transmembrane voltages for homomeric TREK-1 channels (open circles, n = 7) or homomeric TRAAK (solid circles, n = 6). G) Sample traces show two types of single channels recorded at −80 mV from a HEK293 cell co-transfected with TREK-1/eGFP and TRAAK/mCherry plasmids, one type (upper, TREK-1/TRAAK) has unitary currents apparently larger than homomeric channels and another type (lower, TREK-1-like) has unitary currents similar to homomeric TREK-1 channels shown in F. Bottom panel, I-V curves of the currents of TREK-1/TRAAK single channels (n = 12, solid triangles) and TREK-1-like single channels (n = 13, open triangles). H) Summary of single channel conductance at −80 mV (open bars) and 80 mV (closed bars) for homomeric TREK-1 (n = 5 at −80 mV, n = 6 at 80 mV), homomeric TRAAK (n = 6 at both voltages), TREK-1-like (n = 12 at −80 mV, n = 5 at 80 mV), and TREK-1/TRAAK channels (n = 8 at −80 mV, n = 5 at 80 mV) expressed in HEK293 cells. The single channel conductance of nodal <t>K2P</t> channels (n = 13 at both voltages) is also included in the graph for a comparison. The single channel conductance at −80 mV was used for comparison. All recordings were performed under the cell-attached mode. Data represent Mean ± SEM, ns, no significant difference, *p < 0.05, ***p < 0.001, one-way ANOVA with the Tukey post hoc test. See also Fig. S5–9
    Rabbit Anti K2p 10 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti k2p 10 1 - by Bioz Stars, 2023-06
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    Images

    1) Product Images from "TREK-1 and TRAAK are principal K + channels at the nodes of Ranvier for rapid action potential conduction on mammalian myelinated afferent nerves"

    Article Title: TREK-1 and TRAAK are principal K + channels at the nodes of Ranvier for rapid action potential conduction on mammalian myelinated afferent nerves

    Journal: Neuron

    doi: 10.1016/j.neuron.2019.08.042

    A) Left, TREK-1 immunoreactivity (TREK-1-ir) at a NR and MBP immunoreactivity (MBP-ir) on myelin sheath. Right, TREK-1-ir at a NR and CASPR-ir in paranodal regions. B) Left, TRAAK-ir at a NR and MBP-ir on myelin sheath. Right, TRAAK-ir at a NR and CASPR-ir in paranodal regions. C) Similar to A&B except TREK-2-ir was examined and was negative at NRs. In A-C, NRs are indicated by arrows. MBP, myelin basic protein. CASPR, contactin associated protein. D) Summary of immunoreactive nodes for experiments represented in A-C: 112/129 nodes were TREK-1-ir positive, 118/129 nodes were TRAAK-ir positive. 0/129 nodes were TREK-2-ir positive. E) HEK293 cells transfected with TREK-1/eGFP (left), TRAAK/mCherry (middle), and both TREK-1/EGFP and TRAAK/mCherry (right). F) Traces illustrate single channel currents recorded at −80 mV from an HEK293 cell transfected with TREK-1/eGFP (upper, homomeric TREK-1) or an HEK293 cell transfected with TRAAK/mCherry (lower, homomeric TRAAK). Bottom, I-V curves of single channel currents recorded at different transmembrane voltages for homomeric TREK-1 channels (open circles, n = 7) or homomeric TRAAK (solid circles, n = 6). G) Sample traces show two types of single channels recorded at −80 mV from a HEK293 cell co-transfected with TREK-1/eGFP and TRAAK/mCherry plasmids, one type (upper, TREK-1/TRAAK) has unitary currents apparently larger than homomeric channels and another type (lower, TREK-1-like) has unitary currents similar to homomeric TREK-1 channels shown in F. Bottom panel, I-V curves of the currents of TREK-1/TRAAK single channels (n = 12, solid triangles) and TREK-1-like single channels (n = 13, open triangles). H) Summary of single channel conductance at −80 mV (open bars) and 80 mV (closed bars) for homomeric TREK-1 (n = 5 at −80 mV, n = 6 at 80 mV), homomeric TRAAK (n = 6 at both voltages), TREK-1-like (n = 12 at −80 mV, n = 5 at 80 mV), and TREK-1/TRAAK channels (n = 8 at −80 mV, n = 5 at 80 mV) expressed in HEK293 cells. The single channel conductance of nodal K2P channels (n = 13 at both voltages) is also included in the graph for a comparison. The single channel conductance at −80 mV was used for comparison. All recordings were performed under the cell-attached mode. Data represent Mean ± SEM, ns, no significant difference, *p < 0.05, ***p < 0.001, one-way ANOVA with the Tukey post hoc test. See also Fig. S5–9
    Figure Legend Snippet: A) Left, TREK-1 immunoreactivity (TREK-1-ir) at a NR and MBP immunoreactivity (MBP-ir) on myelin sheath. Right, TREK-1-ir at a NR and CASPR-ir in paranodal regions. B) Left, TRAAK-ir at a NR and MBP-ir on myelin sheath. Right, TRAAK-ir at a NR and CASPR-ir in paranodal regions. C) Similar to A&B except TREK-2-ir was examined and was negative at NRs. In A-C, NRs are indicated by arrows. MBP, myelin basic protein. CASPR, contactin associated protein. D) Summary of immunoreactive nodes for experiments represented in A-C: 112/129 nodes were TREK-1-ir positive, 118/129 nodes were TRAAK-ir positive. 0/129 nodes were TREK-2-ir positive. E) HEK293 cells transfected with TREK-1/eGFP (left), TRAAK/mCherry (middle), and both TREK-1/EGFP and TRAAK/mCherry (right). F) Traces illustrate single channel currents recorded at −80 mV from an HEK293 cell transfected with TREK-1/eGFP (upper, homomeric TREK-1) or an HEK293 cell transfected with TRAAK/mCherry (lower, homomeric TRAAK). Bottom, I-V curves of single channel currents recorded at different transmembrane voltages for homomeric TREK-1 channels (open circles, n = 7) or homomeric TRAAK (solid circles, n = 6). G) Sample traces show two types of single channels recorded at −80 mV from a HEK293 cell co-transfected with TREK-1/eGFP and TRAAK/mCherry plasmids, one type (upper, TREK-1/TRAAK) has unitary currents apparently larger than homomeric channels and another type (lower, TREK-1-like) has unitary currents similar to homomeric TREK-1 channels shown in F. Bottom panel, I-V curves of the currents of TREK-1/TRAAK single channels (n = 12, solid triangles) and TREK-1-like single channels (n = 13, open triangles). H) Summary of single channel conductance at −80 mV (open bars) and 80 mV (closed bars) for homomeric TREK-1 (n = 5 at −80 mV, n = 6 at 80 mV), homomeric TRAAK (n = 6 at both voltages), TREK-1-like (n = 12 at −80 mV, n = 5 at 80 mV), and TREK-1/TRAAK channels (n = 8 at −80 mV, n = 5 at 80 mV) expressed in HEK293 cells. The single channel conductance of nodal K2P channels (n = 13 at both voltages) is also included in the graph for a comparison. The single channel conductance at −80 mV was used for comparison. All recordings were performed under the cell-attached mode. Data represent Mean ± SEM, ns, no significant difference, *p < 0.05, ***p < 0.001, one-way ANOVA with the Tukey post hoc test. See also Fig. S5–9

    Techniques Used: Transfection

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, shRNA, Software

    apc 055  (Alomone Labs)


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    Alomone Labs apc 055
    Apc 055, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1 article reviews
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    rabbit anti k2p 10 1  (Alomone Labs)


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    Alomone Labs rabbit anti k2p 10 1
    A) Left, TREK-1 immunoreactivity (TREK-1-ir) at a NR and MBP immunoreactivity (MBP-ir) on myelin sheath. Right, TREK-1-ir at a NR and CASPR-ir in paranodal regions. B) Left, TRAAK-ir at a NR and MBP-ir on myelin sheath. Right, TRAAK-ir at a NR and CASPR-ir in paranodal regions. C) Similar to A&B except TREK-2-ir was examined and was negative at NRs. In A-C, NRs are indicated by arrows. MBP, myelin basic protein. CASPR, contactin associated protein. D) Summary of immunoreactive nodes for experiments represented in A-C: 112/129 nodes were TREK-1-ir positive, 118/129 nodes were TRAAK-ir positive. 0/129 nodes were TREK-2-ir positive. E) HEK293 cells transfected with TREK-1/eGFP (left), TRAAK/mCherry (middle), and both TREK-1/EGFP and TRAAK/mCherry (right). F) Traces illustrate single channel currents recorded at −80 mV from an HEK293 cell transfected with TREK-1/eGFP (upper, homomeric TREK-1) or an HEK293 cell transfected with TRAAK/mCherry (lower, homomeric TRAAK). Bottom, I-V curves of single channel currents recorded at different transmembrane voltages for homomeric TREK-1 channels (open circles, n = 7) or homomeric TRAAK (solid circles, n = 6). G) Sample traces show two types of single channels recorded at −80 mV from a HEK293 cell co-transfected with TREK-1/eGFP and TRAAK/mCherry plasmids, one type (upper, TREK-1/TRAAK) has unitary currents apparently larger than homomeric channels and another type (lower, TREK-1-like) has unitary currents similar to homomeric TREK-1 channels shown in F. Bottom panel, I-V curves of the currents of TREK-1/TRAAK single channels (n = 12, solid triangles) and TREK-1-like single channels (n = 13, open triangles). H) Summary of single channel conductance at −80 mV (open bars) and 80 mV (closed bars) for homomeric TREK-1 (n = 5 at −80 mV, n = 6 at 80 mV), homomeric TRAAK (n = 6 at both voltages), TREK-1-like (n = 12 at −80 mV, n = 5 at 80 mV), and TREK-1/TRAAK channels (n = 8 at −80 mV, n = 5 at 80 mV) expressed in HEK293 cells. The single channel conductance of nodal <t>K2P</t> channels (n = 13 at both voltages) is also included in the graph for a comparison. The single channel conductance at −80 mV was used for comparison. All recordings were performed under the cell-attached mode. Data represent Mean ± SEM, ns, no significant difference, *p < 0.05, ***p < 0.001, one-way ANOVA with the Tukey post hoc test. See also Fig. S5–9
    Rabbit Anti K2p 10 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti k2p 10 1/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti k2p 10 1 - by Bioz Stars, 2023-06
    92/100 stars

    Images

    1) Product Images from "TREK-1 and TRAAK are principal K + channels at the nodes of Ranvier for rapid action potential conduction on mammalian myelinated afferent nerves"

    Article Title: TREK-1 and TRAAK are principal K + channels at the nodes of Ranvier for rapid action potential conduction on mammalian myelinated afferent nerves

    Journal: Neuron

    doi: 10.1016/j.neuron.2019.08.042

    A) Left, TREK-1 immunoreactivity (TREK-1-ir) at a NR and MBP immunoreactivity (MBP-ir) on myelin sheath. Right, TREK-1-ir at a NR and CASPR-ir in paranodal regions. B) Left, TRAAK-ir at a NR and MBP-ir on myelin sheath. Right, TRAAK-ir at a NR and CASPR-ir in paranodal regions. C) Similar to A&B except TREK-2-ir was examined and was negative at NRs. In A-C, NRs are indicated by arrows. MBP, myelin basic protein. CASPR, contactin associated protein. D) Summary of immunoreactive nodes for experiments represented in A-C: 112/129 nodes were TREK-1-ir positive, 118/129 nodes were TRAAK-ir positive. 0/129 nodes were TREK-2-ir positive. E) HEK293 cells transfected with TREK-1/eGFP (left), TRAAK/mCherry (middle), and both TREK-1/EGFP and TRAAK/mCherry (right). F) Traces illustrate single channel currents recorded at −80 mV from an HEK293 cell transfected with TREK-1/eGFP (upper, homomeric TREK-1) or an HEK293 cell transfected with TRAAK/mCherry (lower, homomeric TRAAK). Bottom, I-V curves of single channel currents recorded at different transmembrane voltages for homomeric TREK-1 channels (open circles, n = 7) or homomeric TRAAK (solid circles, n = 6). G) Sample traces show two types of single channels recorded at −80 mV from a HEK293 cell co-transfected with TREK-1/eGFP and TRAAK/mCherry plasmids, one type (upper, TREK-1/TRAAK) has unitary currents apparently larger than homomeric channels and another type (lower, TREK-1-like) has unitary currents similar to homomeric TREK-1 channels shown in F. Bottom panel, I-V curves of the currents of TREK-1/TRAAK single channels (n = 12, solid triangles) and TREK-1-like single channels (n = 13, open triangles). H) Summary of single channel conductance at −80 mV (open bars) and 80 mV (closed bars) for homomeric TREK-1 (n = 5 at −80 mV, n = 6 at 80 mV), homomeric TRAAK (n = 6 at both voltages), TREK-1-like (n = 12 at −80 mV, n = 5 at 80 mV), and TREK-1/TRAAK channels (n = 8 at −80 mV, n = 5 at 80 mV) expressed in HEK293 cells. The single channel conductance of nodal K2P channels (n = 13 at both voltages) is also included in the graph for a comparison. The single channel conductance at −80 mV was used for comparison. All recordings were performed under the cell-attached mode. Data represent Mean ± SEM, ns, no significant difference, *p < 0.05, ***p < 0.001, one-way ANOVA with the Tukey post hoc test. See also Fig. S5–9
    Figure Legend Snippet: A) Left, TREK-1 immunoreactivity (TREK-1-ir) at a NR and MBP immunoreactivity (MBP-ir) on myelin sheath. Right, TREK-1-ir at a NR and CASPR-ir in paranodal regions. B) Left, TRAAK-ir at a NR and MBP-ir on myelin sheath. Right, TRAAK-ir at a NR and CASPR-ir in paranodal regions. C) Similar to A&B except TREK-2-ir was examined and was negative at NRs. In A-C, NRs are indicated by arrows. MBP, myelin basic protein. CASPR, contactin associated protein. D) Summary of immunoreactive nodes for experiments represented in A-C: 112/129 nodes were TREK-1-ir positive, 118/129 nodes were TRAAK-ir positive. 0/129 nodes were TREK-2-ir positive. E) HEK293 cells transfected with TREK-1/eGFP (left), TRAAK/mCherry (middle), and both TREK-1/EGFP and TRAAK/mCherry (right). F) Traces illustrate single channel currents recorded at −80 mV from an HEK293 cell transfected with TREK-1/eGFP (upper, homomeric TREK-1) or an HEK293 cell transfected with TRAAK/mCherry (lower, homomeric TRAAK). Bottom, I-V curves of single channel currents recorded at different transmembrane voltages for homomeric TREK-1 channels (open circles, n = 7) or homomeric TRAAK (solid circles, n = 6). G) Sample traces show two types of single channels recorded at −80 mV from a HEK293 cell co-transfected with TREK-1/eGFP and TRAAK/mCherry plasmids, one type (upper, TREK-1/TRAAK) has unitary currents apparently larger than homomeric channels and another type (lower, TREK-1-like) has unitary currents similar to homomeric TREK-1 channels shown in F. Bottom panel, I-V curves of the currents of TREK-1/TRAAK single channels (n = 12, solid triangles) and TREK-1-like single channels (n = 13, open triangles). H) Summary of single channel conductance at −80 mV (open bars) and 80 mV (closed bars) for homomeric TREK-1 (n = 5 at −80 mV, n = 6 at 80 mV), homomeric TRAAK (n = 6 at both voltages), TREK-1-like (n = 12 at −80 mV, n = 5 at 80 mV), and TREK-1/TRAAK channels (n = 8 at −80 mV, n = 5 at 80 mV) expressed in HEK293 cells. The single channel conductance of nodal K2P channels (n = 13 at both voltages) is also included in the graph for a comparison. The single channel conductance at −80 mV was used for comparison. All recordings were performed under the cell-attached mode. Data represent Mean ± SEM, ns, no significant difference, *p < 0.05, ***p < 0.001, one-way ANOVA with the Tukey post hoc test. See also Fig. S5–9

    Techniques Used: Transfection

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, shRNA, Software

    rabbit anti trek2  (Alomone Labs)


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    Alomone Labs rabbit anti trek2
    Rabbit Anti Trek2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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