Journal: The FASEB Journal
Article Title: BACE1 and presenilin/?-secretase regulate proteolytic processing of KCNE1 and 2, auxiliary subunits of voltage-gated potassium channels
Figure Lengend Snippet: PS/γ-secretase activity regulates generation of KCNE1- and KCNE2-ICDs. A ) Western blot analysis showed that KCNE1-ICD is specifically regulated by PS/γ-secretase activity in B104 cells stably expressing full-length KCNE1. Epoxomicin, a proteasome inhibitor, prevented KCNE1-ICD degradation. B ) KCNE2-ICD is detected in the similar conditions.
Article Snippet: Primary antibodies were used at the following dilutions: GAPDH, 1:1000 (Chemicon, Temecula, CA, USA), BACE1 poly antibody, 1:1000 (Abcam), anti-KCNQ1 antibody, 1:200 (UC Davis/NIH NeuroMab Facility), anti-KCNE1, 1:250 with 5% BSA (Alomone Labs), anti-KCNE2, 1:250 with 1% BSA (Alomone Labs), and anti-V5, 1:5000 with 5% BSA (Invitrogen).
Techniques: Activity Assay, Western Blot, Stable Transfection, Expressing