isradipine  (Alomone Labs)

Journal: bioRxiv

Article Title: Lamellar cells in Pacinian and Meissner corpuscles are touch sensors

doi: 10.1101/2020.08.24.265231

Figure Lengend Snippet: Pharmacological profile of Meissner lamellar cell firing. Quantification of the number of action potentials in response to current injection in the presence of indicated pharmacological agents: 10 µM Felodipine, a mix of 10 µM Nimodipine and 5 µM Isradipine, 10 µM Nifedipine, Agatoxin mix (1 µM ω-Agatoxin IVA and 1 µM ω-Agatoxin TK), Conotoxin mix (5 µM ω-Conotoxin CnVIIA, 10 nM ω-Conotoxin CVIB, 10 nM ω-Conotoxin CVIE, 1 µM ω-Conotoxin MVIIC and 1 µM ω-Conotoxin MVIID), 1 µM SNX-482, 5 µM Mibefradil, 200 nM Kurtoxin. Thin lines represent individual cells, thick lines connect means ± s.e.m. Data were obtained from at least two independent experiments.

Article Snippet: For pharmacological experiments, bath solution was supplemented with the following: 300 µM CdCl2 , 20 µM CaCl2, 10 µM Felodipine (Abcam), a mix of 10 µM Nimodipine and 5 µM Isradipine (Alomone), 10 µM Nifedipine (Alomone), Agatoxin mix (1 µM ω-Agatoxin IVA and 1 µM ω-Agatoxin TK from Alomone), Conotoxin mix (5 µM ω-Conotoxin CnVIIA, 10 nM ω-Conotoxin CVIB, 10 nM ω-Conotoxin CVIE, 1 µM ω-Conotoxin MVIIC and 1 µM ω-Conotoxin MVIID, from Alomone), 1 µM SNX-482 (from Alomone or Peptides International), 5 µM Mibefradil*2HCl, 200 nM Kurtoxin (Alomone), 200 µM Tetrodotoxin citrate (Tocris).

Techniques: Injection

Journal: Molecular psychiatry

Article Title: Cocaine- and stress-primed reinstatement of drug-associated memories elicit differential behavioral and frontostriatal circuit activity patterns via recruitment of L-type Ca2+ channels

doi: 10.1038/s41380-019-0513-2

Figure Lengend Snippet: LTCCs are required for cocaine- and stress-primed reinstatement of cocaine conditioned place preference, a) Experimental timeline of cocaine conditioned place preference (CPP) acquisition, extinction and cocaine-primed (CPR) or stress-primed (SPR) reinstatement protocol and isradipine treatment, b) Prior to treatment with either vehicle (pre-vehicle) or isradipine (pre-isradipine), all mice acquired (* p

Article Snippet: Isradipine (Alomone Labs) was dissolved in saline (16% ethanol) at a concentration of 0.12 mg/ml to be injected i.p. at 0.01 ml/g body weight for a final dosage of 1.2 mg/kg.

Techniques: Conditioned Place Preference, SPR Assay, Mouse Assay

Journal: Molecular psychiatry

Article Title: Cocaine- and stress-primed reinstatement of drug-associated memories elicit differential behavioral and frontostriatal circuit activity patterns via recruitment of L-type Ca2+ channels

doi: 10.1038/s41380-019-0513-2

Figure Lengend Snippet: Cocaine- and stress-primed reinstatement recruit the PrL→NAcC projection, a) C57BL/6J mice were injected with a Cre-dependent AAV expressing GCaMP6s into unilateral PrL and a retrograde AAV expressing Cre recombinase was injected into ipsilateral NAcC. An optic fiber was implanted above the PrL, which could be attached to a patch cord to record Ca 2+ signals, b) Representative image of GCaMP6s expressing in the PrL. Optic fiber location is outlined in white. MO, medial orbitofrontal cortex; Cg, cingulate cortex, c) Experimental timeline for fiber photometry recording during cocaine CPP. Ca 2+ imaging was conducted during the baseline test, acquisition test, extinction test, and CPR/SPR. Mice were treated with vehicle or isradipine to block LTCCs prior to CPR/SPR recording, d) Representative fiber photometry trace of a vehicle-treated mouse during CPR showing Ca 2+ transients prior to cocaine-paired entries (red) but not saline-paired entries (blue). Grey, middle CPP chamber, e) The average amplitude (% ΔF/F) of PrL→NAcC fiber photometry signal 5 seconds prior to entry into the cocaine-paired chamber was significantly higher as compared to entries into the saline-paired chamber in vehicle-treated mice (** p

Article Snippet: Isradipine (Alomone Labs) was dissolved in saline (16% ethanol) at a concentration of 0.12 mg/ml to be injected i.p. at 0.01 ml/g body weight for a final dosage of 1.2 mg/kg.

Techniques: Mouse Assay, Injection, Expressing, Imaging, SPR Assay, Blocking Assay

Journal: Communications Biology

Article Title: Acetyl-CoA-carboxylase 1 (ACC1) plays a critical role in glucagon secretion

doi: 10.1038/s42003-022-03170-w

Figure Lengend Snippet: ACC1 is necessary for the maintenance of voltage-gated calcium currents in alpha cells. Electrophysiological recordings of Ca 2+ currents triggered by a series of 20 ms depolarising pulses (from −70 to +40 mV with a 10 mV step increment) from a holding potential of −70 mV in control (black trace/squares) and gluACC1KO (red trace/circles) alpha cells within intact islets. Current–voltage relationships were fitted with a Boltzmann function. a , b Representative Ca 2+ current trace ( a ) and summary of the amplitude of Ca 2+ currents ( b ) in the presence of 0.1 µg/ml tetrodotoxin to block Na + channels. c Summary of the amplitude of P/Q-type Ca 2+ currents (ω-agatoxin VIA-sensitive component). d Summary of the amplitude of L-type Ca 2+ currents (isradipine-sensitive component). Data from n = 4 independent islet preparations analysed by t -test. All data presented as mean ± SEM. Significance threshold: * P

Article Snippet: TOFA (5-(tetradecyloxy)-2-furoic acid ; ab141578) and Tolbutamide were purchased from Abcam; Noradrenaline from Tocris Bioscience (Bristol, UK); 2-Bromopalmitate from Sigma-Aldrich; Tetrodotoxin, ω-agatoxin and isradipine from Alomone Labs (Jerusalem, Israel).

Techniques: Blocking Assay