trpv2  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Alomone Labs trpv2
    Relative mRNA levels of microglial phagocytic receptors and inflammation factors in microglia cells after knockdown of <t>TRPV2.</t> BV2 microglia were treated with or without CBD (5 μM) after 12 h, and mRNA levels were determined by qPCR. The data are represented as the mean ± SEM ( n = 4). * p
    Trpv2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpv2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpv2 - by Bioz Stars, 2022-12
    94/100 stars

    Images

    1) Product Images from "Cannabidiol Enhances Microglial Beta-Amyloid Peptide Phagocytosis and Clearance via Vanilloid Family Type 2 Channel Activation"

    Article Title: Cannabidiol Enhances Microglial Beta-Amyloid Peptide Phagocytosis and Clearance via Vanilloid Family Type 2 Channel Activation

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms23105367

    Relative mRNA levels of microglial phagocytic receptors and inflammation factors in microglia cells after knockdown of TRPV2. BV2 microglia were treated with or without CBD (5 μM) after 12 h, and mRNA levels were determined by qPCR. The data are represented as the mean ± SEM ( n = 4). * p
    Figure Legend Snippet: Relative mRNA levels of microglial phagocytic receptors and inflammation factors in microglia cells after knockdown of TRPV2. BV2 microglia were treated with or without CBD (5 μM) after 12 h, and mRNA levels were determined by qPCR. The data are represented as the mean ± SEM ( n = 4). * p

    Techniques Used: Real-time Polymerase Chain Reaction

    The TRPV2-mediated phagocytosis in microglia cells was attenuated by inhibiting PDK, Akt, or PERK. FITC-Aβ42 uptake index in BV2 microglia cells was analyzed using the phagocytosis assay. ( A ) The presence of CBD (5 μM), 2APB (250 μM), and CAP (capsaicin: 2 μM) after 24 h ( n = 6). ** p
    Figure Legend Snippet: The TRPV2-mediated phagocytosis in microglia cells was attenuated by inhibiting PDK, Akt, or PERK. FITC-Aβ42 uptake index in BV2 microglia cells was analyzed using the phagocytosis assay. ( A ) The presence of CBD (5 μM), 2APB (250 μM), and CAP (capsaicin: 2 μM) after 24 h ( n = 6). ** p

    Techniques Used: Phagocytosis Assay

    TRPV2 levels were decreased in both AD patients and APP/PS1 mice. ( A ) The TRPV2 levels were measured in the brain whole-protein extracts from mice at the indicated age (E is embryos, P is days and M is months). The data are represented as the mean ± SEM ( n = 4 mice/group). * p
    Figure Legend Snippet: TRPV2 levels were decreased in both AD patients and APP/PS1 mice. ( A ) The TRPV2 levels were measured in the brain whole-protein extracts from mice at the indicated age (E is embryos, P is days and M is months). The data are represented as the mean ± SEM ( n = 4 mice/group). * p

    Techniques Used: Mouse Assay

    CBD enhanced microglial Aβ phagocytosis via the TRPV2 channels. ( A ) Primary microglia cells were plated at a density of 5 × 10 4 in poly-D-lysine-coated wells of 24-well plates containing 10% FBS DMEM medium. After 24 h of treatment with CBD (5 μM), FITC-Aβ42 (1 μg/mL) was added to the medium. Immunofluorescence analysis of the microglial phagocytosis of FITC-Aβ42 was performed after allowing uptake for 4 h in microglia cells. ( B ) Quantification of the internalized FITC-Aβ42 using ImageJ software ( n = 36 to 42 per group). a.u., arbitrary units from 3/4 individual mice. ( C ) FITC-Aβ42 uptake index in the presence or absence of CBD after uptake for the indicated time in microglia cells, calculated based on the phagocytosis assay. ( D ) Microglial Aβ42 uptake was analyzed using Western blotting after 4 h of incubation with Aβ42 oligomer in the presence or absence of CBD. ( E ) Quantification of the protein levels using Image J software ( n = 4 per group). * p
    Figure Legend Snippet: CBD enhanced microglial Aβ phagocytosis via the TRPV2 channels. ( A ) Primary microglia cells were plated at a density of 5 × 10 4 in poly-D-lysine-coated wells of 24-well plates containing 10% FBS DMEM medium. After 24 h of treatment with CBD (5 μM), FITC-Aβ42 (1 μg/mL) was added to the medium. Immunofluorescence analysis of the microglial phagocytosis of FITC-Aβ42 was performed after allowing uptake for 4 h in microglia cells. ( B ) Quantification of the internalized FITC-Aβ42 using ImageJ software ( n = 36 to 42 per group). a.u., arbitrary units from 3/4 individual mice. ( C ) FITC-Aβ42 uptake index in the presence or absence of CBD after uptake for the indicated time in microglia cells, calculated based on the phagocytosis assay. ( D ) Microglial Aβ42 uptake was analyzed using Western blotting after 4 h of incubation with Aβ42 oligomer in the presence or absence of CBD. ( E ) Quantification of the protein levels using Image J software ( n = 4 per group). * p

    Techniques Used: Immunofluorescence, Software, Mouse Assay, Phagocytosis Assay, Western Blot, Incubation

    CBD induced autophagy in microglial cells via TRPV2 by promoting the upregulation of Akt. ( A ) BV2 microglia cells incubated with Aβ42 were analyzed using Western blot in the presence of CBD (5 μM) for the indicated time. ( B ) BV2 microglial cells were pretreated with Tra (tranilast 75 μM) for 1 h and then treated with CBD for 24 h. Band densitometry quantification of TRPV2. The autophagy flux expression was normalized to GAPDH. The phosphorylation of Akt was normalized to the total Akt level ( n = 4 per group). * p
    Figure Legend Snippet: CBD induced autophagy in microglial cells via TRPV2 by promoting the upregulation of Akt. ( A ) BV2 microglia cells incubated with Aβ42 were analyzed using Western blot in the presence of CBD (5 μM) for the indicated time. ( B ) BV2 microglial cells were pretreated with Tra (tranilast 75 μM) for 1 h and then treated with CBD for 24 h. Band densitometry quantification of TRPV2. The autophagy flux expression was normalized to GAPDH. The phosphorylation of Akt was normalized to the total Akt level ( n = 4 per group). * p

    Techniques Used: Incubation, Western Blot, Expressing

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Alomone Labs trpv2
    Relative mRNA levels of microglial phagocytic receptors and inflammation factors in microglia cells after knockdown of <t>TRPV2.</t> BV2 microglia were treated with or without CBD (5 μM) after 12 h, and mRNA levels were determined by qPCR. The data are represented as the mean ± SEM ( n = 4). * p
    Trpv2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpv2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpv2 - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

    Image Search Results


    Relative mRNA levels of microglial phagocytic receptors and inflammation factors in microglia cells after knockdown of TRPV2. BV2 microglia were treated with or without CBD (5 μM) after 12 h, and mRNA levels were determined by qPCR. The data are represented as the mean ± SEM ( n = 4). * p

    Journal: International Journal of Molecular Sciences

    Article Title: Cannabidiol Enhances Microglial Beta-Amyloid Peptide Phagocytosis and Clearance via Vanilloid Family Type 2 Channel Activation

    doi: 10.3390/ijms23105367

    Figure Lengend Snippet: Relative mRNA levels of microglial phagocytic receptors and inflammation factors in microglia cells after knockdown of TRPV2. BV2 microglia were treated with or without CBD (5 μM) after 12 h, and mRNA levels were determined by qPCR. The data are represented as the mean ± SEM ( n = 4). * p

    Article Snippet: The TRPV2 (1:1000, polyclonal antibody, # ALO-ACC-032-50) was purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Real-time Polymerase Chain Reaction

    The TRPV2-mediated phagocytosis in microglia cells was attenuated by inhibiting PDK, Akt, or PERK. FITC-Aβ42 uptake index in BV2 microglia cells was analyzed using the phagocytosis assay. ( A ) The presence of CBD (5 μM), 2APB (250 μM), and CAP (capsaicin: 2 μM) after 24 h ( n = 6). ** p

    Journal: International Journal of Molecular Sciences

    Article Title: Cannabidiol Enhances Microglial Beta-Amyloid Peptide Phagocytosis and Clearance via Vanilloid Family Type 2 Channel Activation

    doi: 10.3390/ijms23105367

    Figure Lengend Snippet: The TRPV2-mediated phagocytosis in microglia cells was attenuated by inhibiting PDK, Akt, or PERK. FITC-Aβ42 uptake index in BV2 microglia cells was analyzed using the phagocytosis assay. ( A ) The presence of CBD (5 μM), 2APB (250 μM), and CAP (capsaicin: 2 μM) after 24 h ( n = 6). ** p

    Article Snippet: The TRPV2 (1:1000, polyclonal antibody, # ALO-ACC-032-50) was purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Phagocytosis Assay

    TRPV2 levels were decreased in both AD patients and APP/PS1 mice. ( A ) The TRPV2 levels were measured in the brain whole-protein extracts from mice at the indicated age (E is embryos, P is days and M is months). The data are represented as the mean ± SEM ( n = 4 mice/group). * p

    Journal: International Journal of Molecular Sciences

    Article Title: Cannabidiol Enhances Microglial Beta-Amyloid Peptide Phagocytosis and Clearance via Vanilloid Family Type 2 Channel Activation

    doi: 10.3390/ijms23105367

    Figure Lengend Snippet: TRPV2 levels were decreased in both AD patients and APP/PS1 mice. ( A ) The TRPV2 levels were measured in the brain whole-protein extracts from mice at the indicated age (E is embryos, P is days and M is months). The data are represented as the mean ± SEM ( n = 4 mice/group). * p

    Article Snippet: The TRPV2 (1:1000, polyclonal antibody, # ALO-ACC-032-50) was purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Mouse Assay

    CBD enhanced microglial Aβ phagocytosis via the TRPV2 channels. ( A ) Primary microglia cells were plated at a density of 5 × 10 4 in poly-D-lysine-coated wells of 24-well plates containing 10% FBS DMEM medium. After 24 h of treatment with CBD (5 μM), FITC-Aβ42 (1 μg/mL) was added to the medium. Immunofluorescence analysis of the microglial phagocytosis of FITC-Aβ42 was performed after allowing uptake for 4 h in microglia cells. ( B ) Quantification of the internalized FITC-Aβ42 using ImageJ software ( n = 36 to 42 per group). a.u., arbitrary units from 3/4 individual mice. ( C ) FITC-Aβ42 uptake index in the presence or absence of CBD after uptake for the indicated time in microglia cells, calculated based on the phagocytosis assay. ( D ) Microglial Aβ42 uptake was analyzed using Western blotting after 4 h of incubation with Aβ42 oligomer in the presence or absence of CBD. ( E ) Quantification of the protein levels using Image J software ( n = 4 per group). * p

    Journal: International Journal of Molecular Sciences

    Article Title: Cannabidiol Enhances Microglial Beta-Amyloid Peptide Phagocytosis and Clearance via Vanilloid Family Type 2 Channel Activation

    doi: 10.3390/ijms23105367

    Figure Lengend Snippet: CBD enhanced microglial Aβ phagocytosis via the TRPV2 channels. ( A ) Primary microglia cells were plated at a density of 5 × 10 4 in poly-D-lysine-coated wells of 24-well plates containing 10% FBS DMEM medium. After 24 h of treatment with CBD (5 μM), FITC-Aβ42 (1 μg/mL) was added to the medium. Immunofluorescence analysis of the microglial phagocytosis of FITC-Aβ42 was performed after allowing uptake for 4 h in microglia cells. ( B ) Quantification of the internalized FITC-Aβ42 using ImageJ software ( n = 36 to 42 per group). a.u., arbitrary units from 3/4 individual mice. ( C ) FITC-Aβ42 uptake index in the presence or absence of CBD after uptake for the indicated time in microglia cells, calculated based on the phagocytosis assay. ( D ) Microglial Aβ42 uptake was analyzed using Western blotting after 4 h of incubation with Aβ42 oligomer in the presence or absence of CBD. ( E ) Quantification of the protein levels using Image J software ( n = 4 per group). * p

    Article Snippet: The TRPV2 (1:1000, polyclonal antibody, # ALO-ACC-032-50) was purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Immunofluorescence, Software, Mouse Assay, Phagocytosis Assay, Western Blot, Incubation

    CBD induced autophagy in microglial cells via TRPV2 by promoting the upregulation of Akt. ( A ) BV2 microglia cells incubated with Aβ42 were analyzed using Western blot in the presence of CBD (5 μM) for the indicated time. ( B ) BV2 microglial cells were pretreated with Tra (tranilast 75 μM) for 1 h and then treated with CBD for 24 h. Band densitometry quantification of TRPV2. The autophagy flux expression was normalized to GAPDH. The phosphorylation of Akt was normalized to the total Akt level ( n = 4 per group). * p

    Journal: International Journal of Molecular Sciences

    Article Title: Cannabidiol Enhances Microglial Beta-Amyloid Peptide Phagocytosis and Clearance via Vanilloid Family Type 2 Channel Activation

    doi: 10.3390/ijms23105367

    Figure Lengend Snippet: CBD induced autophagy in microglial cells via TRPV2 by promoting the upregulation of Akt. ( A ) BV2 microglia cells incubated with Aβ42 were analyzed using Western blot in the presence of CBD (5 μM) for the indicated time. ( B ) BV2 microglial cells were pretreated with Tra (tranilast 75 μM) for 1 h and then treated with CBD for 24 h. Band densitometry quantification of TRPV2. The autophagy flux expression was normalized to GAPDH. The phosphorylation of Akt was normalized to the total Akt level ( n = 4 per group). * p

    Article Snippet: The TRPV2 (1:1000, polyclonal antibody, # ALO-ACC-032-50) was purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Incubation, Western Blot, Expressing