sk3  (Alomone Labs)


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    Structured Review

    Alomone Labs sk3
    Postsynaptic expression of the SK channel subunit, <t>SK3,</t> is reduced after CFA. Average histograms and representative western blots showing protein expression levels of SK1 (lower band), SK2 and SK3 subunits in the total homogenate (TH) and postsynaptic
    Sk3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sk3/product/Alomone Labs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    sk3 - by Bioz Stars, 2022-08
    94/100 stars

    Images

    1) Product Images from "In vivo activation of the SK channel in the spinal cord reduces the NMDA receptor antagonist dose needed to produce antinociception in an inflammatory pain model"

    Article Title: In vivo activation of the SK channel in the spinal cord reduces the NMDA receptor antagonist dose needed to produce antinociception in an inflammatory pain model

    Journal: Pain

    doi: 10.1097/j.pain.0000000000000124

    Postsynaptic expression of the SK channel subunit, SK3, is reduced after CFA. Average histograms and representative western blots showing protein expression levels of SK1 (lower band), SK2 and SK3 subunits in the total homogenate (TH) and postsynaptic
    Figure Legend Snippet: Postsynaptic expression of the SK channel subunit, SK3, is reduced after CFA. Average histograms and representative western blots showing protein expression levels of SK1 (lower band), SK2 and SK3 subunits in the total homogenate (TH) and postsynaptic

    Techniques Used: Expressing, Western Blot

    SK3 channels show both somatic and dendritic expression. A SK3 (green) immunolabeling shows abundant distribution throughout DH laminae (10x). Control (blank) image shows that there was no immunoreaction product in the spinal cord sections in the absence
    Figure Legend Snippet: SK3 channels show both somatic and dendritic expression. A SK3 (green) immunolabeling shows abundant distribution throughout DH laminae (10x). Control (blank) image shows that there was no immunoreaction product in the spinal cord sections in the absence

    Techniques Used: Expressing, Immunolabeling

    SK3-containing SK channels can be co-expressed in close proximity with NMDA receptors. Double immunolabeling with SK3 (green) and NR1 (red) antibodies shows co-expression (yellow) of NR1 subunit of NMDAR and SK3-containing channels within the same neuron
    Figure Legend Snippet: SK3-containing SK channels can be co-expressed in close proximity with NMDA receptors. Double immunolabeling with SK3 (green) and NR1 (red) antibodies shows co-expression (yellow) of NR1 subunit of NMDAR and SK3-containing channels within the same neuron

    Techniques Used: Immunolabeling, Expressing

    2) Product Images from "Molecular and functional characterization of detrusor PDGFRα positive cells in spinal cord injury-induced detrusor overactivity"

    Article Title: Molecular and functional characterization of detrusor PDGFRα positive cells in spinal cord injury-induced detrusor overactivity

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-95781-2

    PDGFRα immunoreactivity of detrusor muscle layer in control and SCI. I mmunoreactivity of PDGFRα (green) and SK3 (red) in control (sham). SCI (24 h, 48 h and 72 h).
    Figure Legend Snippet: PDGFRα immunoreactivity of detrusor muscle layer in control and SCI. I mmunoreactivity of PDGFRα (green) and SK3 (red) in control (sham). SCI (24 h, 48 h and 72 h).

    Techniques Used:

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    Alomone Labs anti cav3 2
    Knockdown or overexpression of TRPC7 did not alter the expression of several important ion channels/pump in NRVMs. a – g Western blots showing the expression of a TRPC7, b HCN4, c Cav1.3, d IP3R1, e Cav3.1, f <t>Cav3.2,</t> g SERCA in NRVMs infected with different adenoviruses to knockdown or overexpress TRPC7. h – n Bar charts showing the quantification of each protein from a – g . To eliminate the loading bias, intensity of each target protein was normalized to that of its corresponding β-tubulin. TRPC7 was successfully knocked down or overexpressed in NRVMs but the change of TRPC7 expression did not alter the expression of HCN4, Cav1.3, IP3R1, Cav3.1, Cav3.2, and SERCA. Data were presented as mean ± SEM ( n = 4). * P
    Anti Cav3 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cav3 2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cav3 2 - by Bioz Stars, 2022-08
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    93
    Alomone Labs anti kcnn3 kca2 3 sk3 n term antibody
    Summary of nucleus accumbens <t>KCNN3</t> transcript expression in ethanol drinking rhesus macaques and C57BL/6J mice. a-c The relative expression of brain KCNN3 transcripts ( <t>SK3_ex7/8</t> , 1 SK3_ex1B , and SK3_ex4 ) among the three drinking macaque groups ( SK3_ex1B : * p = 0.0381 vs CTRL; SK3_ex4 : ** p = 0.0084 vs CTRL, *** p = 0.0009 vs CTRL). d-f The relative expression of the KCNN3 transcripts in drinking monkeys collapsed by age at onset of ethanol drinking ( SK3_ex4 : ** p
    Anti Kcnn3 Kca2 3 Sk3 N Term Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kcnn3 kca2 3 sk3 n term antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kcnn3 kca2 3 sk3 n term antibody - by Bioz Stars, 2022-08
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    94
    Alomone Labs anti kca2 3 atto 594
    Expression of CD140α, CD44, CD34 and <t>SK3</t> cell surface markers in iMSCs, fibroblasts, and PDGFRα-positive cells. Flow cytometry analysis detected the expression of CD140α (( A ). cell count; ( B) . mean fluorescence intensity), CD44 (( C ). cell count; ( D ). mean fluorescence intensity), CD34 (( E ). cell count; ( F ). mean fluorescence intensity), and SK3 (( G ). cell count; ( H ). mean fluorescence intensity) in iMSCs, fibroblasts, and PDGFRα-positive ( n = 3, * p
    Anti Kca2 3 Atto 594, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kca2 3 atto 594/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kca2 3 atto 594 - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    Knockdown or overexpression of TRPC7 did not alter the expression of several important ion channels/pump in NRVMs. a – g Western blots showing the expression of a TRPC7, b HCN4, c Cav1.3, d IP3R1, e Cav3.1, f Cav3.2, g SERCA in NRVMs infected with different adenoviruses to knockdown or overexpress TRPC7. h – n Bar charts showing the quantification of each protein from a – g . To eliminate the loading bias, intensity of each target protein was normalized to that of its corresponding β-tubulin. TRPC7 was successfully knocked down or overexpressed in NRVMs but the change of TRPC7 expression did not alter the expression of HCN4, Cav1.3, IP3R1, Cav3.1, Cav3.2, and SERCA. Data were presented as mean ± SEM ( n = 4). * P

    Journal: Stem Cell Research & Therapy

    Article Title: TRPC7 regulates the electrophysiological functions of embryonic stem cell-derived cardiomyocytes

    doi: 10.1186/s13287-021-02308-7

    Figure Lengend Snippet: Knockdown or overexpression of TRPC7 did not alter the expression of several important ion channels/pump in NRVMs. a – g Western blots showing the expression of a TRPC7, b HCN4, c Cav1.3, d IP3R1, e Cav3.1, f Cav3.2, g SERCA in NRVMs infected with different adenoviruses to knockdown or overexpress TRPC7. h – n Bar charts showing the quantification of each protein from a – g . To eliminate the loading bias, intensity of each target protein was normalized to that of its corresponding β-tubulin. TRPC7 was successfully knocked down or overexpressed in NRVMs but the change of TRPC7 expression did not alter the expression of HCN4, Cav1.3, IP3R1, Cav3.1, Cav3.2, and SERCA. Data were presented as mean ± SEM ( n = 4). * P

    Article Snippet: Antibodies used were anti-TRPC7 1:500 (HPA031126, Sigma), anti-β-tubulin 1:1000 (15,115, Cell Signaling, Danvers, Massachusetts, USA), anti-HCN4 1:200 (APC-052, Alomone), anti-Cav1.3 1:100 (ACC-005, Alomone), anti-Cav3.1 1:200 (ACC-021, Alomone), anti-Cav3.2 1:200 (ACC-025, Alomone), anti-RyR2 1:1000 (MA3-916, Invitrogen), anti-SERCA 1:200 (sc-30110, Santa Cruz), anti-IP3R 1:500 (ACC-019, Alomone), anti-p(S2814)RyR2 1:5000 (A010-31, Badrilla), anti-phospholamban (PLN) 1:1000 (A010-14, Badrilla), anti-p(T17) PLN 1:5000 (A010-13, Badrilla), HRP-conjugated goat anti-rabbit secondary antibody 1:5000 (Dako, Zug, Switzerland), and HRP-conjugated goat anti-mouse secondary antibody 1:5000 (Dako).

    Techniques: Over Expression, Expressing, Western Blot, Infection

    Summary of nucleus accumbens KCNN3 transcript expression in ethanol drinking rhesus macaques and C57BL/6J mice. a-c The relative expression of brain KCNN3 transcripts ( SK3_ex7/8 , 1 SK3_ex1B , and SK3_ex4 ) among the three drinking macaque groups ( SK3_ex1B : * p = 0.0381 vs CTRL; SK3_ex4 : ** p = 0.0084 vs CTRL, *** p = 0.0009 vs CTRL). d-f The relative expression of the KCNN3 transcripts in drinking monkeys collapsed by age at onset of ethanol drinking ( SK3_ex4 : ** p

    Journal: bioRxiv

    Article Title: Cross-species epigenetic regulation of nucleus accumbens KCNN3 transcript variants by excessive ethanol drinking and dependence

    doi: 10.1101/713826

    Figure Lengend Snippet: Summary of nucleus accumbens KCNN3 transcript expression in ethanol drinking rhesus macaques and C57BL/6J mice. a-c The relative expression of brain KCNN3 transcripts ( SK3_ex7/8 , 1 SK3_ex1B , and SK3_ex4 ) among the three drinking macaque groups ( SK3_ex1B : * p = 0.0381 vs CTRL; SK3_ex4 : ** p = 0.0084 vs CTRL, *** p = 0.0009 vs CTRL). d-f The relative expression of the KCNN3 transcripts in drinking monkeys collapsed by age at onset of ethanol drinking ( SK3_ex4 : ** p

    Article Snippet: We first performed a series of western blots using different titrations of sample and antibody to establish the linear range for KCa 2.3 (Alomone Labs, Jerusalem, Israel; Catalog #: APC-025; epitope AA 2-21 of human KCa 2.3) in primate tissue samples.

    Techniques: Expressing, Mouse Assay

    Adaptations in nucleus accumbens KCNN3 transcript and protein expression in ethanol drinking female rhesus macaques. a-c The relative expression of KCNN3 transcripts ( SK3_ex7/8 , 1 SK3_ex1B , and SK3_ex4 ) among control and very heavy drinking macaque groups ( SK3_ex1B : * p = 0.037 vs CTRL; SK3_ex4 : * p = 0.024 vs CTRL). d Characterization of anti-K Ca 2.3 channel western blot in macaque accumbens tissue (protein loading range, 1.25 – 40 µg). e Positive correlation between the amount of protein loaded and anti-K Ca 2.3 channel optical density values. f,g The full K Ca 2.3 channel blot and quantitation of normalized K Ca 2.3 channel protein expression in controls and drinkers (* p = 0.0324 vs CTRL).

    Journal: bioRxiv

    Article Title: Cross-species epigenetic regulation of nucleus accumbens KCNN3 transcript variants by excessive ethanol drinking and dependence

    doi: 10.1101/713826

    Figure Lengend Snippet: Adaptations in nucleus accumbens KCNN3 transcript and protein expression in ethanol drinking female rhesus macaques. a-c The relative expression of KCNN3 transcripts ( SK3_ex7/8 , 1 SK3_ex1B , and SK3_ex4 ) among control and very heavy drinking macaque groups ( SK3_ex1B : * p = 0.037 vs CTRL; SK3_ex4 : * p = 0.024 vs CTRL). d Characterization of anti-K Ca 2.3 channel western blot in macaque accumbens tissue (protein loading range, 1.25 – 40 µg). e Positive correlation between the amount of protein loaded and anti-K Ca 2.3 channel optical density values. f,g The full K Ca 2.3 channel blot and quantitation of normalized K Ca 2.3 channel protein expression in controls and drinkers (* p = 0.0324 vs CTRL).

    Article Snippet: We first performed a series of western blots using different titrations of sample and antibody to establish the linear range for KCa 2.3 (Alomone Labs, Jerusalem, Israel; Catalog #: APC-025; epitope AA 2-21 of human KCa 2.3) in primate tissue samples.

    Techniques: Expressing, Western Blot, Quantitation Assay

    KCNN3 methylation levels within MR-ex1-200 of ethanol drinking monkeys and dependent mice. The average methylation rates of individual CpGs included in the methylation region under study are shown. a Exon organization of the KCNN3 locus showing the location of exons and the dual CAG trinucleotide repeat arrays and methylated region in exon 1 (MR-ex1). b In rhesus macaque, the following CpGs showed elevated rates of methylation in heavy/very heavy drinking macaques vs controls: CpG 129130376 : F(2, 11.846) = 7.9, * p = 0.04; CpG 129130680 : F(2, 15.955) = 3.661, * p = 0.036; CpG 129130739 : F(2, 15.468) = 3.817, * p = 0.04; CpG 129130770 : F(2, 13.57) = 5.047, * p = 0.041; CpG 129130792 : F(2, 13.945) = 7.047, * p = 0.01; CpG 129130816 : F(2, 15.63) = 5.836, * p = 0.015; CpG 129130832 : F(2, 15.473) = 3.95, * p = 0.033; CpG 129130931 : F(2, 14.393) = 4.016, * p = 0.038; CpG 129130964 : F(2, 15.708) = 3.985, * p = 0.031. c In female macaques, elevated rates of methylation were observed at the following CpGs in very heavy drinking macaques vs controls: CpG 129130612 : F(1, 7) = 6.122, * p = 0.048; CpG 129130632 : F(1, 7) = 7.64, * p = 0.033; CpG 129130685 : F(1, 7) = 13.370, * p = 0.011; CpG 129130699 : F(1, 7) = 7.799, * p = 0.031. d In mouse accumbens, the following CpGs showed elevated rates of methylation between non-drinking mice and drinking dependent mice: Independent t-tests: CpG 89555945, t (16)= −2.946, *** p = 0.0009; CpG 89555974, t (17) = −2.860, * p = 0.011; CpG 89556220, t (17) = −2.206, * p = 0.042.

    Journal: bioRxiv

    Article Title: Cross-species epigenetic regulation of nucleus accumbens KCNN3 transcript variants by excessive ethanol drinking and dependence

    doi: 10.1101/713826

    Figure Lengend Snippet: KCNN3 methylation levels within MR-ex1-200 of ethanol drinking monkeys and dependent mice. The average methylation rates of individual CpGs included in the methylation region under study are shown. a Exon organization of the KCNN3 locus showing the location of exons and the dual CAG trinucleotide repeat arrays and methylated region in exon 1 (MR-ex1). b In rhesus macaque, the following CpGs showed elevated rates of methylation in heavy/very heavy drinking macaques vs controls: CpG 129130376 : F(2, 11.846) = 7.9, * p = 0.04; CpG 129130680 : F(2, 15.955) = 3.661, * p = 0.036; CpG 129130739 : F(2, 15.468) = 3.817, * p = 0.04; CpG 129130770 : F(2, 13.57) = 5.047, * p = 0.041; CpG 129130792 : F(2, 13.945) = 7.047, * p = 0.01; CpG 129130816 : F(2, 15.63) = 5.836, * p = 0.015; CpG 129130832 : F(2, 15.473) = 3.95, * p = 0.033; CpG 129130931 : F(2, 14.393) = 4.016, * p = 0.038; CpG 129130964 : F(2, 15.708) = 3.985, * p = 0.031. c In female macaques, elevated rates of methylation were observed at the following CpGs in very heavy drinking macaques vs controls: CpG 129130612 : F(1, 7) = 6.122, * p = 0.048; CpG 129130632 : F(1, 7) = 7.64, * p = 0.033; CpG 129130685 : F(1, 7) = 13.370, * p = 0.011; CpG 129130699 : F(1, 7) = 7.799, * p = 0.031. d In mouse accumbens, the following CpGs showed elevated rates of methylation between non-drinking mice and drinking dependent mice: Independent t-tests: CpG 89555945, t (16)= −2.946, *** p = 0.0009; CpG 89555974, t (17) = −2.860, * p = 0.011; CpG 89556220, t (17) = −2.206, * p = 0.042.

    Article Snippet: We first performed a series of western blots using different titrations of sample and antibody to establish the linear range for KCa 2.3 (Alomone Labs, Jerusalem, Israel; Catalog #: APC-025; epitope AA 2-21 of human KCa 2.3) in primate tissue samples.

    Techniques: Methylation, Mouse Assay

    KCNN3 -CAG n allele frequency distribution among male and female rhesus macaques. a The frequency distribution of (CAG) n alleles is shown for low drinkers (LD), binge drinkers (BD), heavy drinkers (HD), and very heavy drinkers (VHD). b Correlation between CAG repeat sum and average ethanol intake. c-e Correlations between CAG repeat sum and KCNN3 transcript expression in long-term drinking rhesus macaques.

    Journal: bioRxiv

    Article Title: Cross-species epigenetic regulation of nucleus accumbens KCNN3 transcript variants by excessive ethanol drinking and dependence

    doi: 10.1101/713826

    Figure Lengend Snippet: KCNN3 -CAG n allele frequency distribution among male and female rhesus macaques. a The frequency distribution of (CAG) n alleles is shown for low drinkers (LD), binge drinkers (BD), heavy drinkers (HD), and very heavy drinkers (VHD). b Correlation between CAG repeat sum and average ethanol intake. c-e Correlations between CAG repeat sum and KCNN3 transcript expression in long-term drinking rhesus macaques.

    Article Snippet: We first performed a series of western blots using different titrations of sample and antibody to establish the linear range for KCa 2.3 (Alomone Labs, Jerusalem, Israel; Catalog #: APC-025; epitope AA 2-21 of human KCa 2.3) in primate tissue samples.

    Techniques: Expressing

    Anti-SK3, IK1 and caveolin-1 Western blot analysis of anti-caveolin-1 immunoprecipitates prepared from porcine coronary artery endothelium. Immunoprecipitation (IP) was performed with and without anti-caveolin-1 antibody (+ve and −ve,

    Journal:

    Article Title: Effects of methyl ?-cyclodextrin on EDHF responses in pig and rat arteries; association between SKCa channels and caveolin-rich domains

    doi: 10.1038/sj.bjp.0707222

    Figure Lengend Snippet: Anti-SK3, IK1 and caveolin-1 Western blot analysis of anti-caveolin-1 immunoprecipitates prepared from porcine coronary artery endothelium. Immunoprecipitation (IP) was performed with and without anti-caveolin-1 antibody (+ve and −ve,

    Article Snippet: Antibodies used were anti-SK3 (APC-025; Alomone Laboratories, Jerusalem, Israel), anti-IK1 (a kind gift of Dr M Chen, GlaxoSmithKline), anti-caveolin 1 mAb (clone 2234; BD Transduction Laboratories, San Diego, CA, USA), anti-caveolin 1 pAb (sc-894, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and anti-eNOS (610298, BD Transduction Laboratories).

    Techniques: Western Blot, Immunoprecipitation

    Expression of CD140α, CD44, CD34 and SK3 cell surface markers in iMSCs, fibroblasts, and PDGFRα-positive cells. Flow cytometry analysis detected the expression of CD140α (( A ). cell count; ( B) . mean fluorescence intensity), CD44 (( C ). cell count; ( D ). mean fluorescence intensity), CD34 (( E ). cell count; ( F ). mean fluorescence intensity), and SK3 (( G ). cell count; ( H ). mean fluorescence intensity) in iMSCs, fibroblasts, and PDGFRα-positive ( n = 3, * p

    Journal: International Journal of Molecular Sciences

    Article Title: Differentiated PDGFRα-Positive Cells: A Novel In-Vitro Model for Functional Studies of Neuronal Nitric Oxide Synthase

    doi: 10.3390/ijms22073514

    Figure Lengend Snippet: Expression of CD140α, CD44, CD34 and SK3 cell surface markers in iMSCs, fibroblasts, and PDGFRα-positive cells. Flow cytometry analysis detected the expression of CD140α (( A ). cell count; ( B) . mean fluorescence intensity), CD44 (( C ). cell count; ( D ). mean fluorescence intensity), CD34 (( E ). cell count; ( F ). mean fluorescence intensity), and SK3 (( G ). cell count; ( H ). mean fluorescence intensity) in iMSCs, fibroblasts, and PDGFRα-positive ( n = 3, * p

    Article Snippet: Cells were then washed with PBS for 10 min and incubated with the following primary antibodies in a humidified chamber overnight at 4 °C: anti-CD34 (ab81289; Abcam, Cambridge, UK), anti-CD44 (BD Pharmingen, San Diego, CA, USA), anti-KCa2.3-ATTO-594 (SK3; Alomone Labs, Jerusalem, Israel), anti-PDGFRα (ab234965; Abcam, Cambridge, UK), and anti-nNOS (3G6B10; Invitrogen Carlsbad, CA, USA).

    Techniques: Expressing, Flow Cytometry, Cell Counting, Fluorescence