nav1 α subunit specific antibodies  (alomone labs)


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    alomone labs nav1 α subunit specific antibodies
    Co-localization of FGF14 and <t>Nav1.6</t> in mouse cortex using 1% formaldehyde and 0.5% MeOH fixation . (A–D) The gray and red channels represent FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody, the green channel represents PanNav (Sigma-Aldrich, rabbit anti PanNav, catalog number S6936) in (A) , Nav1.1 (Alomone Labs) in (B) , Nav1.2 (Alomone Labs) in (C) , and Nav1.6 (Alomone Labs) in (D) visualized with an Alexa 488-conjugated secondary antibody and the blue represents Topro3 nuclear staining in the cortex. Right panels represent overlaid images (third column from the left) and high magnification of boxed ROI from the merged images. Scale bars represent 20 μm.
    Nav1 α Subunit Specific Antibodies, supplied by alomone labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nav1 α subunit specific antibodies/product/alomone labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nav1 α subunit specific antibodies - by Bioz Stars, 2022-05
    86/100 stars

    Images

    1) Product Images from "Improved Methods for Fluorescence Microscopy Detection of Macromolecules at the Axon Initial Segment"

    Article Title: Improved Methods for Fluorescence Microscopy Detection of Macromolecules at the Axon Initial Segment

    Journal: Frontiers in Cellular Neuroscience

    doi: 10.3389/fncel.2016.00005

    Co-localization of FGF14 and Nav1.6 in mouse cortex using 1% formaldehyde and 0.5% MeOH fixation . (A–D) The gray and red channels represent FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody, the green channel represents PanNav (Sigma-Aldrich, rabbit anti PanNav, catalog number S6936) in (A) , Nav1.1 (Alomone Labs) in (B) , Nav1.2 (Alomone Labs) in (C) , and Nav1.6 (Alomone Labs) in (D) visualized with an Alexa 488-conjugated secondary antibody and the blue represents Topro3 nuclear staining in the cortex. Right panels represent overlaid images (third column from the left) and high magnification of boxed ROI from the merged images. Scale bars represent 20 μm.
    Figure Legend Snippet: Co-localization of FGF14 and Nav1.6 in mouse cortex using 1% formaldehyde and 0.5% MeOH fixation . (A–D) The gray and red channels represent FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody, the green channel represents PanNav (Sigma-Aldrich, rabbit anti PanNav, catalog number S6936) in (A) , Nav1.1 (Alomone Labs) in (B) , Nav1.2 (Alomone Labs) in (C) , and Nav1.6 (Alomone Labs) in (D) visualized with an Alexa 488-conjugated secondary antibody and the blue represents Topro3 nuclear staining in the cortex. Right panels represent overlaid images (third column from the left) and high magnification of boxed ROI from the merged images. Scale bars represent 20 μm.

    Techniques Used: Staining

    Evaluation of the 1% formaldehyde + 0.5% MeOH fixation method for post-hoc analysis in acute brain slices . (A–C) The gray channel represents FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents Nav1.6 (Alomone Labs) visualized with an Alexa 488-conjugated secondary antibody, and the blue represents Ankyrin-G (NeuroMab, catalog number 75-146) visualized with an Alexa 647-conjugated secondary antibody in the cortex at low in (A,C) and high in (B) magnification. Images in (A,B) are from the cortex while images in (C) are taken from the NAc. (D) The red channel represents Nav1.6 (Alomone Labs) immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents Ankyrin-G (NeuroMab, catalog number 75–146) visualized with an Alexa 488-conjugated secondary antibody and the blue represents NeuN (visualized with an Alexa 647-conjugated secondary antibody) in the NAc. Arrows show FGF14 and/or Nav1.6 signals at the axon initial segment (AIS). NAc, nucleus accumbens. Scale bars represent 20 μm.
    Figure Legend Snippet: Evaluation of the 1% formaldehyde + 0.5% MeOH fixation method for post-hoc analysis in acute brain slices . (A–C) The gray channel represents FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents Nav1.6 (Alomone Labs) visualized with an Alexa 488-conjugated secondary antibody, and the blue represents Ankyrin-G (NeuroMab, catalog number 75-146) visualized with an Alexa 647-conjugated secondary antibody in the cortex at low in (A,C) and high in (B) magnification. Images in (A,B) are from the cortex while images in (C) are taken from the NAc. (D) The red channel represents Nav1.6 (Alomone Labs) immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents Ankyrin-G (NeuroMab, catalog number 75–146) visualized with an Alexa 488-conjugated secondary antibody and the blue represents NeuN (visualized with an Alexa 647-conjugated secondary antibody) in the NAc. Arrows show FGF14 and/or Nav1.6 signals at the axon initial segment (AIS). NAc, nucleus accumbens. Scale bars represent 20 μm.

    Techniques Used:

    Representative examples of double immunofluorescence staining of mouse brain fresh-frozen sections followed by the indicated post-fixative treatments. (A) Weak detection of FGF14 immunoreactivity at the AIS (arrow) in cells in the mouse cortical region using 1% PFA post-fixed treatment (Scheme 1 , Option A, first column of Table 1 ). (B) Weak detection of FGF14 immunoreactivity at the AIS (arrow) in cells of the mouse cortical region using 4% PFA post-fixed treatment (Scheme 1 , Option A, second column of Table 1 ). (C) Optimal detection of FGF14 immunoreactivity in the CA3 hippocampal region following acetone-based brief post-fixation treatment (Scheme 1 , Option A, third column of Table 1 ). (D) Representative confocal images of double immunostaining of the DG using acetone-based post-fixation treatment. The gray and red channels represent FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents βIV-spectrin immunoreactivity visualized with an Alexa 488-conjugated secondary antibody. Arrows show co-localization between FGF14 and βIV-spectrin at the AIS. Green and red channel overlay images are shown on the right. (E) Representative confocal images of double immunostaining of the NAc using acetone-based (without methanol) post-fixation treatment (Scheme 1 , Option A, third column of Table 1 ). The gray and red channels represent FGF14 immunoreactivity, the green channel represents Nav1.6 (primary antibody from Alomone Labs) visualized (weakly) with an Alexa 488-conjugated secondary antibody. The blue represents Topro-3 nuclear staining shown in the green, red, and blue image overlay on the right. Arrows show co-localization between FGF14 and Nav1.6 at the AIS. (F) Representative confocal images of double immunostaining of a zoomed area of the CA1 hippocampal region using acetone + methanol-based post-fixation treatment (Scheme 1 , Option A, fourth column of Table 1 ). The gray and red channels represent FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents parvalbumin immunoreactivity visualized with an Alexa 488-conjugated secondary antibody. Arrows show localization of FGF14 at the AIS in areas around the parvalbumin soma. Green and red channel overlay images are shown on the right. Note an FGF14 positive halo overlays with somatic parvalbumin staining suggesting localized co-expression of the two proteins in cytoplasmic regions. Arrows indicate FGF14, βIV-spectrin, and/or Nav1.6 signals at the axon initial segment (AIS). DG, dentate gyrus; NAc, nucleus accumbens; PFA, paraformaldehyde. Scale bars represent 20 μm.
    Figure Legend Snippet: Representative examples of double immunofluorescence staining of mouse brain fresh-frozen sections followed by the indicated post-fixative treatments. (A) Weak detection of FGF14 immunoreactivity at the AIS (arrow) in cells in the mouse cortical region using 1% PFA post-fixed treatment (Scheme 1 , Option A, first column of Table 1 ). (B) Weak detection of FGF14 immunoreactivity at the AIS (arrow) in cells of the mouse cortical region using 4% PFA post-fixed treatment (Scheme 1 , Option A, second column of Table 1 ). (C) Optimal detection of FGF14 immunoreactivity in the CA3 hippocampal region following acetone-based brief post-fixation treatment (Scheme 1 , Option A, third column of Table 1 ). (D) Representative confocal images of double immunostaining of the DG using acetone-based post-fixation treatment. The gray and red channels represent FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents βIV-spectrin immunoreactivity visualized with an Alexa 488-conjugated secondary antibody. Arrows show co-localization between FGF14 and βIV-spectrin at the AIS. Green and red channel overlay images are shown on the right. (E) Representative confocal images of double immunostaining of the NAc using acetone-based (without methanol) post-fixation treatment (Scheme 1 , Option A, third column of Table 1 ). The gray and red channels represent FGF14 immunoreactivity, the green channel represents Nav1.6 (primary antibody from Alomone Labs) visualized (weakly) with an Alexa 488-conjugated secondary antibody. The blue represents Topro-3 nuclear staining shown in the green, red, and blue image overlay on the right. Arrows show co-localization between FGF14 and Nav1.6 at the AIS. (F) Representative confocal images of double immunostaining of a zoomed area of the CA1 hippocampal region using acetone + methanol-based post-fixation treatment (Scheme 1 , Option A, fourth column of Table 1 ). The gray and red channels represent FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents parvalbumin immunoreactivity visualized with an Alexa 488-conjugated secondary antibody. Arrows show localization of FGF14 at the AIS in areas around the parvalbumin soma. Green and red channel overlay images are shown on the right. Note an FGF14 positive halo overlays with somatic parvalbumin staining suggesting localized co-expression of the two proteins in cytoplasmic regions. Arrows indicate FGF14, βIV-spectrin, and/or Nav1.6 signals at the axon initial segment (AIS). DG, dentate gyrus; NAc, nucleus accumbens; PFA, paraformaldehyde. Scale bars represent 20 μm.

    Techniques Used: Double Immunofluorescence Staining, Double Immunostaining, Staining, Expressing

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    alomone labs nav1 α subunit specific antibodies
    Co-localization of FGF14 and <t>Nav1.6</t> in mouse cortex using 1% formaldehyde and 0.5% MeOH fixation . (A–D) The gray and red channels represent FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody, the green channel represents PanNav (Sigma-Aldrich, rabbit anti PanNav, catalog number S6936) in (A) , Nav1.1 (Alomone Labs) in (B) , Nav1.2 (Alomone Labs) in (C) , and Nav1.6 (Alomone Labs) in (D) visualized with an Alexa 488-conjugated secondary antibody and the blue represents Topro3 nuclear staining in the cortex. Right panels represent overlaid images (third column from the left) and high magnification of boxed ROI from the merged images. Scale bars represent 20 μm.
    Nav1 α Subunit Specific Antibodies, supplied by alomone labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nav1 α subunit specific antibodies/product/alomone labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nav1 α subunit specific antibodies - by Bioz Stars, 2022-05
    86/100 stars
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    Co-localization of FGF14 and Nav1.6 in mouse cortex using 1% formaldehyde and 0.5% MeOH fixation . (A–D) The gray and red channels represent FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody, the green channel represents PanNav (Sigma-Aldrich, rabbit anti PanNav, catalog number S6936) in (A) , Nav1.1 (Alomone Labs) in (B) , Nav1.2 (Alomone Labs) in (C) , and Nav1.6 (Alomone Labs) in (D) visualized with an Alexa 488-conjugated secondary antibody and the blue represents Topro3 nuclear staining in the cortex. Right panels represent overlaid images (third column from the left) and high magnification of boxed ROI from the merged images. Scale bars represent 20 μm.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Improved Methods for Fluorescence Microscopy Detection of Macromolecules at the Axon Initial Segment

    doi: 10.3389/fncel.2016.00005

    Figure Lengend Snippet: Co-localization of FGF14 and Nav1.6 in mouse cortex using 1% formaldehyde and 0.5% MeOH fixation . (A–D) The gray and red channels represent FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody, the green channel represents PanNav (Sigma-Aldrich, rabbit anti PanNav, catalog number S6936) in (A) , Nav1.1 (Alomone Labs) in (B) , Nav1.2 (Alomone Labs) in (C) , and Nav1.6 (Alomone Labs) in (D) visualized with an Alexa 488-conjugated secondary antibody and the blue represents Topro3 nuclear staining in the cortex. Right panels represent overlaid images (third column from the left) and high magnification of boxed ROI from the merged images. Scale bars represent 20 μm.

    Article Snippet: Supplementary Figure 4 FGF14 and Nav1 α-subunit-specific antibodies staining perfusion-fixed tissue preparations (1% formaldehyde +0.5% MeOH) followed by light acetone fixation. (A–C) Representative immunostaining of FGF14 (gray and red) in combination with PanNav (Alomone Labs) in the cortex A, Nav1.2 (NeuroMab) in the subiculum (B) , and cerebellum (C) , all shown in green.

    Techniques: Staining

    Evaluation of the 1% formaldehyde + 0.5% MeOH fixation method for post-hoc analysis in acute brain slices . (A–C) The gray channel represents FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents Nav1.6 (Alomone Labs) visualized with an Alexa 488-conjugated secondary antibody, and the blue represents Ankyrin-G (NeuroMab, catalog number 75-146) visualized with an Alexa 647-conjugated secondary antibody in the cortex at low in (A,C) and high in (B) magnification. Images in (A,B) are from the cortex while images in (C) are taken from the NAc. (D) The red channel represents Nav1.6 (Alomone Labs) immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents Ankyrin-G (NeuroMab, catalog number 75–146) visualized with an Alexa 488-conjugated secondary antibody and the blue represents NeuN (visualized with an Alexa 647-conjugated secondary antibody) in the NAc. Arrows show FGF14 and/or Nav1.6 signals at the axon initial segment (AIS). NAc, nucleus accumbens. Scale bars represent 20 μm.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Improved Methods for Fluorescence Microscopy Detection of Macromolecules at the Axon Initial Segment

    doi: 10.3389/fncel.2016.00005

    Figure Lengend Snippet: Evaluation of the 1% formaldehyde + 0.5% MeOH fixation method for post-hoc analysis in acute brain slices . (A–C) The gray channel represents FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents Nav1.6 (Alomone Labs) visualized with an Alexa 488-conjugated secondary antibody, and the blue represents Ankyrin-G (NeuroMab, catalog number 75-146) visualized with an Alexa 647-conjugated secondary antibody in the cortex at low in (A,C) and high in (B) magnification. Images in (A,B) are from the cortex while images in (C) are taken from the NAc. (D) The red channel represents Nav1.6 (Alomone Labs) immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents Ankyrin-G (NeuroMab, catalog number 75–146) visualized with an Alexa 488-conjugated secondary antibody and the blue represents NeuN (visualized with an Alexa 647-conjugated secondary antibody) in the NAc. Arrows show FGF14 and/or Nav1.6 signals at the axon initial segment (AIS). NAc, nucleus accumbens. Scale bars represent 20 μm.

    Article Snippet: Supplementary Figure 4 FGF14 and Nav1 α-subunit-specific antibodies staining perfusion-fixed tissue preparations (1% formaldehyde +0.5% MeOH) followed by light acetone fixation. (A–C) Representative immunostaining of FGF14 (gray and red) in combination with PanNav (Alomone Labs) in the cortex A, Nav1.2 (NeuroMab) in the subiculum (B) , and cerebellum (C) , all shown in green.

    Techniques:

    Representative examples of double immunofluorescence staining of mouse brain fresh-frozen sections followed by the indicated post-fixative treatments. (A) Weak detection of FGF14 immunoreactivity at the AIS (arrow) in cells in the mouse cortical region using 1% PFA post-fixed treatment (Scheme 1 , Option A, first column of Table 1 ). (B) Weak detection of FGF14 immunoreactivity at the AIS (arrow) in cells of the mouse cortical region using 4% PFA post-fixed treatment (Scheme 1 , Option A, second column of Table 1 ). (C) Optimal detection of FGF14 immunoreactivity in the CA3 hippocampal region following acetone-based brief post-fixation treatment (Scheme 1 , Option A, third column of Table 1 ). (D) Representative confocal images of double immunostaining of the DG using acetone-based post-fixation treatment. The gray and red channels represent FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents βIV-spectrin immunoreactivity visualized with an Alexa 488-conjugated secondary antibody. Arrows show co-localization between FGF14 and βIV-spectrin at the AIS. Green and red channel overlay images are shown on the right. (E) Representative confocal images of double immunostaining of the NAc using acetone-based (without methanol) post-fixation treatment (Scheme 1 , Option A, third column of Table 1 ). The gray and red channels represent FGF14 immunoreactivity, the green channel represents Nav1.6 (primary antibody from Alomone Labs) visualized (weakly) with an Alexa 488-conjugated secondary antibody. The blue represents Topro-3 nuclear staining shown in the green, red, and blue image overlay on the right. Arrows show co-localization between FGF14 and Nav1.6 at the AIS. (F) Representative confocal images of double immunostaining of a zoomed area of the CA1 hippocampal region using acetone + methanol-based post-fixation treatment (Scheme 1 , Option A, fourth column of Table 1 ). The gray and red channels represent FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents parvalbumin immunoreactivity visualized with an Alexa 488-conjugated secondary antibody. Arrows show localization of FGF14 at the AIS in areas around the parvalbumin soma. Green and red channel overlay images are shown on the right. Note an FGF14 positive halo overlays with somatic parvalbumin staining suggesting localized co-expression of the two proteins in cytoplasmic regions. Arrows indicate FGF14, βIV-spectrin, and/or Nav1.6 signals at the axon initial segment (AIS). DG, dentate gyrus; NAc, nucleus accumbens; PFA, paraformaldehyde. Scale bars represent 20 μm.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Improved Methods for Fluorescence Microscopy Detection of Macromolecules at the Axon Initial Segment

    doi: 10.3389/fncel.2016.00005

    Figure Lengend Snippet: Representative examples of double immunofluorescence staining of mouse brain fresh-frozen sections followed by the indicated post-fixative treatments. (A) Weak detection of FGF14 immunoreactivity at the AIS (arrow) in cells in the mouse cortical region using 1% PFA post-fixed treatment (Scheme 1 , Option A, first column of Table 1 ). (B) Weak detection of FGF14 immunoreactivity at the AIS (arrow) in cells of the mouse cortical region using 4% PFA post-fixed treatment (Scheme 1 , Option A, second column of Table 1 ). (C) Optimal detection of FGF14 immunoreactivity in the CA3 hippocampal region following acetone-based brief post-fixation treatment (Scheme 1 , Option A, third column of Table 1 ). (D) Representative confocal images of double immunostaining of the DG using acetone-based post-fixation treatment. The gray and red channels represent FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents βIV-spectrin immunoreactivity visualized with an Alexa 488-conjugated secondary antibody. Arrows show co-localization between FGF14 and βIV-spectrin at the AIS. Green and red channel overlay images are shown on the right. (E) Representative confocal images of double immunostaining of the NAc using acetone-based (without methanol) post-fixation treatment (Scheme 1 , Option A, third column of Table 1 ). The gray and red channels represent FGF14 immunoreactivity, the green channel represents Nav1.6 (primary antibody from Alomone Labs) visualized (weakly) with an Alexa 488-conjugated secondary antibody. The blue represents Topro-3 nuclear staining shown in the green, red, and blue image overlay on the right. Arrows show co-localization between FGF14 and Nav1.6 at the AIS. (F) Representative confocal images of double immunostaining of a zoomed area of the CA1 hippocampal region using acetone + methanol-based post-fixation treatment (Scheme 1 , Option A, fourth column of Table 1 ). The gray and red channels represent FGF14 immunoreactivity visualized with an Alexa 568-conjugated secondary antibody. The green channel represents parvalbumin immunoreactivity visualized with an Alexa 488-conjugated secondary antibody. Arrows show localization of FGF14 at the AIS in areas around the parvalbumin soma. Green and red channel overlay images are shown on the right. Note an FGF14 positive halo overlays with somatic parvalbumin staining suggesting localized co-expression of the two proteins in cytoplasmic regions. Arrows indicate FGF14, βIV-spectrin, and/or Nav1.6 signals at the axon initial segment (AIS). DG, dentate gyrus; NAc, nucleus accumbens; PFA, paraformaldehyde. Scale bars represent 20 μm.

    Article Snippet: Supplementary Figure 4 FGF14 and Nav1 α-subunit-specific antibodies staining perfusion-fixed tissue preparations (1% formaldehyde +0.5% MeOH) followed by light acetone fixation. (A–C) Representative immunostaining of FGF14 (gray and red) in combination with PanNav (Alomone Labs) in the cortex A, Nav1.2 (NeuroMab) in the subiculum (B) , and cerebellum (C) , all shown in green.

    Techniques: Double Immunofluorescence Staining, Double Immunostaining, Staining, Expressing