trpc6 specific antiserum  (Alomone Labs)

Journal:

Article Title: Increased Vascular Smooth Muscle Contractility in TRPC6−/− Mice

doi: 10.1128/MCB.25.16.6980-6989.2005

Figure Lengend Snippet: Vascular smooth muscle properties in wild-type and TRPC6 − / − mice. Agonist-induced vasoconstriction of aortic rings (A and B) and mesenteric arteries (C), telemetric measurement of mean blood pressure (D), and vasoconstriction in response

Article Snippet: PCRs were carried out using the following conditions: initial denaturation for 3 min at 94°C and 45 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min, followed by a final extension at 72°C for 7 min. Western blot analysis of proteins from embryonic brain was carried out as described previously ( ) using a TRPC6-specific antiserum (Alomone Labs, Jerusalem, Israel).

Techniques: Mouse Assay

Journal:

Article Title: Increased Vascular Smooth Muscle Contractility in TRPC6−/− Mice

doi: 10.1128/MCB.25.16.6980-6989.2005

Figure Lengend Snippet: Targeted disruption of the murine TRPC6 gene. (A) cDNA with delineation of intron-exon boundaries and location of hydrophobic segments presumed to code for transmembrane domains, genomic organization of the TRPC6 gene, and restriction maps of the mTRPC6

Article Snippet: PCRs were carried out using the following conditions: initial denaturation for 3 min at 94°C and 45 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min, followed by a final extension at 72°C for 7 min. Western blot analysis of proteins from embryonic brain was carried out as described previously ( ) using a TRPC6-specific antiserum (Alomone Labs, Jerusalem, Israel).

Techniques:

Journal:

Article Title: Increased Vascular Smooth Muscle Contractility in TRPC6−/− Mice

doi: 10.1128/MCB.25.16.6980-6989.2005

Figure Lengend Snippet: Ba 2+ influx into isolated smooth muscle cells from control and TRPC6 − / − mice. (A) Ba 2+ influx into isolated smooth muscle cells from thoracic aortas of control and TRPC6 − / − mice. Isolated cells were loaded

Article Snippet: PCRs were carried out using the following conditions: initial denaturation for 3 min at 94°C and 45 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min, followed by a final extension at 72°C for 7 min. Western blot analysis of proteins from embryonic brain was carried out as described previously ( ) using a TRPC6-specific antiserum (Alomone Labs, Jerusalem, Israel).

Techniques: Isolation, Mouse Assay

Journal:

Article Title: Increased Vascular Smooth Muscle Contractility in TRPC6−/− Mice

doi: 10.1128/MCB.25.16.6980-6989.2005

Figure Lengend Snippet: Quantitative analysis of mRNA levels by real-time PCR of total RNA from thoracic aortas (A) and cerebral arteries (B). Total RNA was prepared from WT (thoracic aortas, n = 4; cerebral arteries, n = 4, black bars) and TRPC6 − / −

Article Snippet: PCRs were carried out using the following conditions: initial denaturation for 3 min at 94°C and 45 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min, followed by a final extension at 72°C for 7 min. Western blot analysis of proteins from embryonic brain was carried out as described previously ( ) using a TRPC6-specific antiserum (Alomone Labs, Jerusalem, Israel).

Techniques: Real-time Polymerase Chain Reaction

Journal:

Article Title: Increased Vascular Smooth Muscle Contractility in TRPC6−/− Mice

doi: 10.1128/MCB.25.16.6980-6989.2005

Figure Lengend Snippet: (A and B) Analysis of current densities by electrophysiological analysis of single smooth muscle cells isolated from cerebral arteries of control (black traces) or TRPC6 − / − (gray traces) mice in the absence (A, left panel) or presence

Article Snippet: PCRs were carried out using the following conditions: initial denaturation for 3 min at 94°C and 45 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min, followed by a final extension at 72°C for 7 min. Western blot analysis of proteins from embryonic brain was carried out as described previously ( ) using a TRPC6-specific antiserum (Alomone Labs, Jerusalem, Israel).

Techniques: Isolation, Mouse Assay