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Alomone Labs vglut2
$AMPAR distribution in adult PF–PC, CF–PC, and PF–interneuron synapses. A , Immunolabeling for GluRδ2 (10 nm particles) is observed in IMP clusters found on the E-face of PC spines, showing very homogeneous labeling within individual synapses and also in different synapses. B , Immunolabeling for AMPAR (5 nm) with high (left) or low (right) densities of immunogold is observed in PF–PC synapses identified by labeling for GluRδ2 (15 nm). C , Some synapses are fractured with partial synaptic areas (open arrow), whereas others are flattened and fractured completely, showing whole areas of synapses (arrows). D , E , Localization of AMPAR labeling (5 nm) in the CF–PC synapses identified by labeling for <t>VGluT2</t> (15 nm) on opposing P-face of presynaptic membrane in the molecular layer. Note that the central area of E was not shadowed with platinum. This makes the 5 nm gold easily discerned on IMPs (above bottom center), but they are more difficult to discern when the IMPs were shadowed with platinum (right side). F , Immunolabeling for Kv4.3 (15 nm) is diffusely observed on the P-face of interneurons. Smaller particles are for mGluR1α, which is not related to this report. G , Immunolabeling for AMPARs (5 nm) is dense and homogeneous in IMP clusters (arrows) in E-face of interneurons identified by matching the complementary P-face shown in F , which is labeled for Kv4.3. Inset shows one of the synapses (open arrow). Rotary shadowing was used for this figure. Scale bars, A–C , F , G , 250 nm; D , E , 100 nm.$
Vglut2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Number and Density of AMPA Receptors in Individual Synapses in the Rat Cerebellum as Revealed by SDS-Digested Freeze-Fracture Replica Labeling"

Article Title: Number and Density of AMPA Receptors in Individual Synapses in the Rat Cerebellum as Revealed by SDS-Digested Freeze-Fracture Replica Labeling

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.2861-06.2007

$AMPAR distribution in adult PF–PC, CF–PC, and PF–interneuron synapses. A , Immunolabeling for GluRδ2 (10 nm particles) is observed in IMP clusters found on the E-face of PC spines, showing very homogeneous labeling within individual synapses and also in different synapses. B , Immunolabeling for AMPAR (5 nm) with high (left) or low (right) densities of immunogold is observed in PF–PC synapses identified by labeling for GluRδ2 (15 nm). C , Some synapses are fractured with partial synaptic areas (open arrow), whereas others are flattened and fractured completely, showing whole areas of synapses (arrows). D , E , Localization of AMPAR labeling (5 nm) in the CF–PC synapses identified by labeling for VGluT2 (15 nm) on opposing P-face of presynaptic membrane in the molecular layer. Note that the central area of E was not shadowed with platinum. This makes the 5 nm gold easily discerned on IMPs (above bottom center), but they are more difficult to discern when the IMPs were shadowed with platinum (right side). F , Immunolabeling for Kv4.3 (15 nm) is diffusely observed on the P-face of interneurons. Smaller particles are for mGluR1α, which is not related to this report. G , Immunolabeling for AMPARs (5 nm) is dense and homogeneous in IMP clusters (arrows) in E-face of interneurons identified by matching the complementary P-face shown in F , which is labeled for Kv4.3. Inset shows one of the synapses (open arrow). Rotary shadowing was used for this figure. Scale bars, A–C , F , G , 250 nm; D , E , 100 nm.$
Figure Legend Snippet: AMPAR distribution in adult PF–PC, CF–PC, and PF–interneuron synapses. A , Immunolabeling for GluRδ2 (10 nm particles) is observed in IMP clusters found on the E-face of PC spines, showing very homogeneous labeling within individual synapses and also in different synapses. B , Immunolabeling for AMPAR (5 nm) with high (left) or low (right) densities of immunogold is observed in PF–PC synapses identified by labeling for GluRδ2 (15 nm). C , Some synapses are fractured with partial synaptic areas (open arrow), whereas others are flattened and fractured completely, showing whole areas of synapses (arrows). D , E , Localization of AMPAR labeling (5 nm) in the CF–PC synapses identified by labeling for VGluT2 (15 nm) on opposing P-face of presynaptic membrane in the molecular layer. Note that the central area of E was not shadowed with platinum. This makes the 5 nm gold easily discerned on IMPs (above bottom center), but they are more difficult to discern when the IMPs were shadowed with platinum (right side). F , Immunolabeling for Kv4.3 (15 nm) is diffusely observed on the P-face of interneurons. Smaller particles are for mGluR1α, which is not related to this report. G , Immunolabeling for AMPARs (5 nm) is dense and homogeneous in IMP clusters (arrows) in E-face of interneurons identified by matching the complementary P-face shown in F , which is labeled for Kv4.3. Inset shows one of the synapses (open arrow). Rotary shadowing was used for this figure. Scale bars, A–C , F , G , 250 nm; D , E , 100 nm.

Techniques Used: Immunolabeling, Labeling

Localization of AMPARs visualized by postembedding triple labeling. A , Immunolabeling for AMPARs (5 nm gold particles; open arrow) is found in some PF–PC synapses but not in others (arrowhead). PF varicosities were identified by labeling for VGluT1 (10 nm gold particles; arrows). B , C , Dense immunolabeling for AMPARs (open arrows) is found in CF–PC synapses identified by labeling for VGluT2 (15 nm gold particles) in terminals (T). D , E , Dense immunolabeling for AMPARs (open arrows) was found in PF–interneuron synapses identified by labeling for VGluT1 (arrows) in terminals. A , Inset, B , C , and D , E are consecutive ultrathin sections. Scale bars, 250 nm ( B–E to same scale).

Figure Legend Snippet: Localization of AMPARs visualized by postembedding triple labeling. A , Immunolabeling for AMPARs (5 nm gold particles; open arrow) is found in some PF–PC synapses but not in others (arrowhead). PF varicosities were identified by labeling for VGluT1 (10 nm gold particles; arrows). B , C , Dense immunolabeling for AMPARs (open arrows) is found in CF–PC synapses identified by labeling for VGluT2 (15 nm gold particles) in terminals (T). D , E , Dense immunolabeling for AMPARs (open arrows) was found in PF–interneuron synapses identified by labeling for VGluT1 (arrows) in terminals. A , Inset, B , C , and D , E are consecutive ultrathin sections. Scale bars, 250 nm ( B–E to same scale).

Techniques Used: Labeling, Immunolabeling

2) Product Images from "Optogenetic stimulation of Glutamatergic Neuronal Activity in the Striatum Enhances Neurogenesis in the Subventricular Zone of Normal and Stroke Mice"

Article Title: Optogenetic stimulation of Glutamatergic Neuronal Activity in the Striatum Enhances Neurogenesis in the Subventricular Zone of Normal and Stroke Mice

Journal: Neurobiology of disease

doi: 10.1016/j.nbd.2016.11.005

Glutamatergic neurons and axonal terminals from the striatum and glutamate release in the SVZ A. Confocal imaging of immunostaining was performed in brain sections from wild type C57/B6L mice. The overlapped neurofilament (NF) and VGLUT2 markers and their co-expression with DAPI identified a glutamatergic neuron in the striatum. B and C. Sagittal brain striatum sections show VGLUT2 and NF expressing axons extending to the adjacent SVZ. Images in C show the box area in panel B. The enlarged images show co-localization of VGLUT2 and neurofilament (NF) in the striatum (arrow head), and extension of these nerve fibers toward the SVZ. D. Immunostaining for DCX, the glutamatergic marker VGLUT1 and the presynapse marker Synapsin-1 in the SVZ, and striatum. The merged image revealed dense distribution of the pre-synaptic protein Synapsin-1 in the striatum/SVZ bordering area. The enlarged image from the box area illustrates a few possible synapses co-labeled with Synapsin-1 and DCX + cells (arrows); most of Synapsin-1 staining, however, located in close proximity of blue colored neuroblasts. E . Axon tracker DiI imaging 14 days after injection into the striatum. The DiI (red) distribution (arrows) due to axonal trafficking was seen along the SVZ and the border region between SVZ and striatum. Representative of brain sections from 5 WT animals.

Figure Legend Snippet: Glutamatergic neurons and axonal terminals from the striatum and glutamate release in the SVZ A. Confocal imaging of immunostaining was performed in brain sections from wild type C57/B6L mice. The overlapped neurofilament (NF) and VGLUT2 markers and their co-expression with DAPI identified a glutamatergic neuron in the striatum. B and C. Sagittal brain striatum sections show VGLUT2 and NF expressing axons extending to the adjacent SVZ. Images in C show the box area in panel B. The enlarged images show co-localization of VGLUT2 and neurofilament (NF) in the striatum (arrow head), and extension of these nerve fibers toward the SVZ. D. Immunostaining for DCX, the glutamatergic marker VGLUT1 and the presynapse marker Synapsin-1 in the SVZ, and striatum. The merged image revealed dense distribution of the pre-synaptic protein Synapsin-1 in the striatum/SVZ bordering area. The enlarged image from the box area illustrates a few possible synapses co-labeled with Synapsin-1 and DCX + cells (arrows); most of Synapsin-1 staining, however, located in close proximity of blue colored neuroblasts. E . Axon tracker DiI imaging 14 days after injection into the striatum. The DiI (red) distribution (arrows) due to axonal trafficking was seen along the SVZ and the border region between SVZ and striatum. Representative of brain sections from 5 WT animals.

Techniques Used: Imaging, Immunostaining, Mouse Assay, Expressing, Marker, Labeling, Staining, Injection

ChR2-YFP expression in the glutamatergic axons and neurons in the cortex and striatum A. Immunofluorescence images from brain sections show expression of ChR2-YFP (green) in the cortex and striatum of the ChR2 transgenic mouse. The characteristic patch-matrix structure was seen in the striatum. B to D. In brain sections from ChR2-YFP transgenic mice, immunofluorescence staining shows co-localization of ChR2-YFP with the excitatory glutamatergic neuron marker VGLUT1 (B), VGLUT2 (C) and CaMKIIα (D), demonstrating that ChR2-YFP-expressing neurons (arrows) and fibers have glutamatergic phenotype residing within the cortex and striatum.

Figure Legend Snippet: ChR2-YFP expression in the glutamatergic axons and neurons in the cortex and striatum A. Immunofluorescence images from brain sections show expression of ChR2-YFP (green) in the cortex and striatum of the ChR2 transgenic mouse. The characteristic patch-matrix structure was seen in the striatum. B to D. In brain sections from ChR2-YFP transgenic mice, immunofluorescence staining shows co-localization of ChR2-YFP with the excitatory glutamatergic neuron marker VGLUT1 (B), VGLUT2 (C) and CaMKIIα (D), demonstrating that ChR2-YFP-expressing neurons (arrows) and fibers have glutamatergic phenotype residing within the cortex and striatum.

Techniques Used: Expressing, Immunofluorescence, Transgenic Assay, Mouse Assay, Staining, Marker

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Journal: The Journal of Neuroscience

Article Title: Number and Density of AMPA Receptors in Individual Synapses in the Rat Cerebellum as Revealed by SDS-Digested Freeze-Fracture Replica Labeling

doi: 10.1523/JNEUROSCI.2861-06.2007

Figure Lengend Snippet: AMPAR distribution in adult PF–PC, CF–PC, and PF–interneuron synapses. A , Immunolabeling for GluRδ2 (10 nm particles) is observed in IMP clusters found on the E-face of PC spines, showing very homogeneous labeling within individual synapses and also in different synapses. B , Immunolabeling for AMPAR (5 nm) with high (left) or low (right) densities of immunogold is observed in PF–PC synapses identified by labeling for GluRδ2 (15 nm). C , Some synapses are fractured with partial synaptic areas (open arrow), whereas others are flattened and fractured completely, showing whole areas of synapses (arrows). D , E , Localization of AMPAR labeling (5 nm) in the CF–PC synapses identified by labeling for VGluT2 (15 nm) on opposing P-face of presynaptic membrane in the molecular layer. Note that the central area of E was not shadowed with platinum. This makes the 5 nm gold easily discerned on IMPs (above bottom center), but they are more difficult to discern when the IMPs were shadowed with platinum (right side). F , Immunolabeling for Kv4.3 (15 nm) is diffusely observed on the P-face of interneurons. Smaller particles are for mGluR1α, which is not related to this report. G , Immunolabeling for AMPARs (5 nm) is dense and homogeneous in IMP clusters (arrows) in E-face of interneurons identified by matching the complementary P-face shown in F , which is labeled for Kv4.3. Inset shows one of the synapses (open arrow). Rotary shadowing was used for this figure. Scale bars, A–C , F , G , 250 nm; D , E , 100 nm.

Article Snippet: The specificity of pan-AMPA , GluR2 , VGluT1 and VGluT2 , and Kv4.3 ( ) (Alomone Labs) antibodies has been extensively characterized previously.

Techniques: Immunolabeling, Labeling

Journal: The Journal of Neuroscience

Article Title: Number and Density of AMPA Receptors in Individual Synapses in the Rat Cerebellum as Revealed by SDS-Digested Freeze-Fracture Replica Labeling

doi: 10.1523/JNEUROSCI.2861-06.2007

Figure Lengend Snippet: Localization of AMPARs visualized by postembedding triple labeling. A , Immunolabeling for AMPARs (5 nm gold particles; open arrow) is found in some PF–PC synapses but not in others (arrowhead). PF varicosities were identified by labeling for VGluT1 (10 nm gold particles; arrows). B , C , Dense immunolabeling for AMPARs (open arrows) is found in CF–PC synapses identified by labeling for VGluT2 (15 nm gold particles) in terminals (T). D , E , Dense immunolabeling for AMPARs (open arrows) was found in PF–interneuron synapses identified by labeling for VGluT1 (arrows) in terminals. A , Inset, B , C , and D , E are consecutive ultrathin sections. Scale bars, 250 nm ( B–E to same scale).

Article Snippet: The specificity of pan-AMPA , GluR2 , VGluT1 and VGluT2 , and Kv4.3 ( ) (Alomone Labs) antibodies has been extensively characterized previously.

Techniques: Labeling, Immunolabeling

Journal: Neurobiology of disease

Article Title: Optogenetic stimulation of Glutamatergic Neuronal Activity in the Striatum Enhances Neurogenesis in the Subventricular Zone of Normal and Stroke Mice

doi: 10.1016/j.nbd.2016.11.005

Figure Lengend Snippet: Glutamatergic neurons and axonal terminals from the striatum and glutamate release in the SVZ A. Confocal imaging of immunostaining was performed in brain sections from wild type C57/B6L mice. The overlapped neurofilament (NF) and VGLUT2 markers and their co-expression with DAPI identified a glutamatergic neuron in the striatum. B and C. Sagittal brain striatum sections show VGLUT2 and NF expressing axons extending to the adjacent SVZ. Images in C show the box area in panel B. The enlarged images show co-localization of VGLUT2 and neurofilament (NF) in the striatum (arrow head), and extension of these nerve fibers toward the SVZ. D. Immunostaining for DCX, the glutamatergic marker VGLUT1 and the presynapse marker Synapsin-1 in the SVZ, and striatum. The merged image revealed dense distribution of the pre-synaptic protein Synapsin-1 in the striatum/SVZ bordering area. The enlarged image from the box area illustrates a few possible synapses co-labeled with Synapsin-1 and DCX + cells (arrows); most of Synapsin-1 staining, however, located in close proximity of blue colored neuroblasts. E . Axon tracker DiI imaging 14 days after injection into the striatum. The DiI (red) distribution (arrows) due to axonal trafficking was seen along the SVZ and the border region between SVZ and striatum. Representative of brain sections from 5 WT animals.

Article Snippet: Immunofluorescence staining was performed with the primary antibodies against VGLUT1 (1:400; UC Davis/NIH NeuroMab Facility), VGLUT2 (1:400; Alomone Labs, Israel.)

Techniques: Imaging, Immunostaining, Mouse Assay, Expressing, Marker, Labeling, Staining, Injection

Journal: Neurobiology of disease

Article Title: Optogenetic stimulation of Glutamatergic Neuronal Activity in the Striatum Enhances Neurogenesis in the Subventricular Zone of Normal and Stroke Mice

doi: 10.1016/j.nbd.2016.11.005

Figure Lengend Snippet: ChR2-YFP expression in the glutamatergic axons and neurons in the cortex and striatum A. Immunofluorescence images from brain sections show expression of ChR2-YFP (green) in the cortex and striatum of the ChR2 transgenic mouse. The characteristic patch-matrix structure was seen in the striatum. B to D. In brain sections from ChR2-YFP transgenic mice, immunofluorescence staining shows co-localization of ChR2-YFP with the excitatory glutamatergic neuron marker VGLUT1 (B), VGLUT2 (C) and CaMKIIα (D), demonstrating that ChR2-YFP-expressing neurons (arrows) and fibers have glutamatergic phenotype residing within the cortex and striatum.

Article Snippet: Immunofluorescence staining was performed with the primary antibodies against VGLUT1 (1:400; UC Davis/NIH NeuroMab Facility), VGLUT2 (1:400; Alomone Labs, Israel.)

Techniques: Expressing, Immunofluorescence, Transgenic Assay, Mouse Assay, Staining, Marker