polyclonal anti vglut2 antibody (Alomone Labs)


Structured Review

Polyclonal Anti Vglut2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal anti vglut2 antibody/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Sparse genetically defined neurons refine the canonical role of periaqueductal gray columnar organization"
Article Title: Sparse genetically defined neurons refine the canonical role of periaqueductal gray columnar organization
Journal: eLife
doi: 10.7554/eLife.77115

Figure Legend Snippet: ( A ) Example histology images showing immunostaining of pan-neuronal marker NeuN (top row), viral-mediated expression of GFP in CCK-expressing cells (middle row), and overlay of NeuN and CCK-GFP (bottom row) in the dorsolateral (left column), lateral (middle column), and ventrolateral (right column) PAG. Scale bars, 10 μm. ( B ) Raw counts of CCK-GFP+ and NeuN+ cells in the dorsolateral PAG (dlPAG), lPAG, and vlPAG. ( C ) Fraction of NeuN-labeled cells that are also GFP-labeled in the dlPAG and l/vlPAG. CCK-expressing cells comprise ~5% of l/vlPAG neurons and constitute significantly more of l/vlPAG neurons than dlPAG neurons (n = 4; paired t-test, ** P =.0032). ( D ) Immunostaining of glutamatergic marker vGlut2 in CCK cells. Example histology images showing vGlut2 (top), CCK-GFP (middle) and vGlut2/GFP overlay (bottom). White arrow indicates vGlut2+/GFP+ cell. Dashed outline indicates vGlut2+/GFP- cell. Scale bar, 10 μm. ( E ) 9.6% of vGlut2-labeled cells in the l/vlPAG are also GFP-labeled (n = 4; 302 vGlut2/GFP+ of 3115 vGlut2+ cells). ( F ) A majority (94.8%) of GFP-labeled cells in the l/vlPAG are also vGlut2-labeled (n = 4; 302 vGlut2+/GFP+ of 317 GFP+ cells). Errorbars: mean ± SEM.
Techniques Used: Immunostaining, Marker, Expressing, Labeling

Figure Legend Snippet: ( A ) In situ hybridization labeling of l/vlPAG neurons showing mRNAs for CCK (green) and VGLUT2 (red). Nuclei stained by DAPI are shown in blue. Example image (×40 objective) of vglut2-labeled cells (red rectangle) and double-labeled vglut2/CCK-labeled (green rectangle) in l/vlPAG. Scale bar, 20 µm. ( B ) Zoomed-in images of subregions in ( A ). Scale bar, 20 µm. ( C ) 8.58% of vglut2-labeled cells are also CCK-labeled (15 of 183 total cells). Errorbars: mean ± SEM.
Techniques Used: In Situ Hybridization, Labeling, Staining
polyclonal anti vglut2 antibody (Alomone Labs)


Structured Review

Polyclonal Anti Vglut2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal anti vglut2 antibody/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Sparse genetically defined neurons refine the canonical role of periaqueductal gray columnar organization"
Article Title: Sparse genetically defined neurons refine the canonical role of periaqueductal gray columnar organization
Journal: eLife
doi: 10.7554/eLife.77115

Figure Legend Snippet: ( A ) Example histology images showing immunostaining of pan-neuronal marker NeuN (top row), viral-mediated expression of GFP in CCK-expressing cells (middle row), and overlay of NeuN and CCK-GFP (bottom row) in the dorsolateral (left column), lateral (middle column), and ventrolateral (right column) PAG. Scale bars, 10 μm. ( B ) Raw counts of CCK-GFP+ and NeuN+ cells in the dorsolateral PAG (dlPAG), lPAG, and vlPAG. ( C ) Fraction of NeuN-labeled cells that are also GFP-labeled in the dlPAG and l/vlPAG. CCK-expressing cells comprise ~5% of l/vlPAG neurons and constitute significantly more of l/vlPAG neurons than dlPAG neurons (n = 4; paired t-test, ** P =.0032). ( D ) Immunostaining of glutamatergic marker vGlut2 in CCK cells. Example histology images showing vGlut2 (top), CCK-GFP (middle) and vGlut2/GFP overlay (bottom). White arrow indicates vGlut2+/GFP+ cell. Dashed outline indicates vGlut2+/GFP- cell. Scale bar, 10 μm. ( E ) 9.6% of vGlut2-labeled cells in the l/vlPAG are also GFP-labeled (n = 4; 302 vGlut2/GFP+ of 3115 vGlut2+ cells). ( F ) A majority (94.8%) of GFP-labeled cells in the l/vlPAG are also vGlut2-labeled (n = 4; 302 vGlut2+/GFP+ of 317 GFP+ cells). Errorbars: mean ± SEM.
Techniques Used: Immunostaining, Marker, Expressing, Labeling

Figure Legend Snippet: ( A ) In situ hybridization labeling of l/vlPAG neurons showing mRNAs for CCK (green) and VGLUT2 (red). Nuclei stained by DAPI are shown in blue. Example image (×40 objective) of vglut2-labeled cells (red rectangle) and double-labeled vglut2/CCK-labeled (green rectangle) in l/vlPAG. Scale bar, 20 µm. ( B ) Zoomed-in images of subregions in ( A ). Scale bar, 20 µm. ( C ) 8.58% of vglut2-labeled cells are also CCK-labeled (15 of 183 total cells). Errorbars: mean ± SEM.
Techniques Used: In Situ Hybridization, Labeling, Staining
polyclonal anti vglut2 antibody (Alomone Labs)


Structured Review

Polyclonal Anti Vglut2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal anti vglut2 antibody/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "A genetically-defined population in the lateral and ventrolateral periaqueductal gray selectively promotes flight to safety"
Article Title: A genetically-defined population in the lateral and ventrolateral periaqueductal gray selectively promotes flight to safety
Journal: bioRxiv
doi: 10.1101/2022.01.19.476981

Figure Legend Snippet: (A) Example histology images showing immunostaining of pan-neuronal marker NeuN (top row), viral-mediated expression of GFP in cck-expressing cells (middle row), and overlay of NeuN and cck-GFP (bottom row) in the dorsolateral (left column), lateral (middle column), and ventrolateral (right column) PAG. Scale bars, 10μm. (B) Raw counts of cck-GFP+ and NeuN+ cells in the dlPAG, lPAG, and vlPAG. (C) Fraction of NeuN-labeled cells that are also GFP-labeled in the dlPAG and l/vlPAG. Cck-expressing cells comprise ∼5% of l/vlPAG neurons and constitute significantly more of l/vlPAG neurons than dlPAG neurons (n=4; paired t-test, **p < .01). (D) Immunostaining of glutamatergic marker vGlut2 in cck cells. Example histology images showing vGlut2 (top), cck-GFP (middle) and vGlut2/GFP overlay (bottom). White arrow indicates vGlut2+/GFP+ cell. Dashed outline indicates vGlut2+/GFP-cell. Scale bar, 10μm. (E) Approximately 95% of GFP-labeled cells are also vGlut2-labeled (n = 4). Mean ± SEM.
Techniques Used: Immunostaining, Marker, Expressing, Labeling
anti vglut2 (Alomone Labs)


Structured Review

Anti Vglut2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti vglut2/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "nNOS-Expressing Neurons in the Ventral Tegmental Area and Substantia Nigra Pars Compacta"
Article Title: nNOS-Expressing Neurons in the Ventral Tegmental Area and Substantia Nigra Pars Compacta
Journal: eNeuro
doi: 10.1523/ENEURO.0381-18.2018

Figure Legend Snippet: Primary antibodies
Techniques Used: Concentration Assay
vglut2 (Alomone Labs)


Structured Review

Vglut2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vglut2/product/Alomone Labs
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Sema7A/PlxnCl signaling triggers activity-dependent olfactory synapse formation"
Article Title: Sema7A/PlxnCl signaling triggers activity-dependent olfactory synapse formation
Journal: Nature Communications
doi: 10.1038/s41467-018-04239-z

Figure Legend Snippet: Synapse formation and dendrite maturation in the Sema7A and PlxnC1 KOs. a Synapse markers within glomeruli in the Sema7A KO. OB sections of the WT and Sema7A KO at P5 and 8w were immunostained with antibodies against vGlut2 and GluR1, pre- and post-synaptic markers, respectively. Staining photos are shown for P5 sections. Signal intensities within the rI7 glomeruli were normalized by the pre-synaptic signals in the ONL and post synaptic signals in the EPL. Fluorescent signals of GL/ONL and GL/EPL in glomeruli are compared between the WT and Sema7A KO. Scale bars=25 μm. * p < 0.05, ** p < 0.01 (Student’s t -test). Error bars indicate SD ( n = 5, 6, 2, 3 animals). b Dendrite morphology of M/T cells in the Sema7A KO. EYFP-tagged rI7 glomeruli (green) were detected by immunostaining with anti-GFP antibodies. M/T cells (yellow) were visualized by Lucifer yellow (LY) injection into the rI7 glomeruli of the WT and Sema7A KO at P5 (left). The numbers of M/T cells with one dendrite (mature) and those with multiple dendrites (immature) to GL were counted for the rI7 glomeruli. The ratios (%) of mature (blue) and immature (white) M/T cells are shown (right): WT, 26/34 (76.5 %); KO, 8/34 cells (23.5 %). n = 20 glomeruli. Scale bar=10 μm. c Synapse markers within glomeruli in the PlxnC1 cKO. OB sections of the WT and PlxnC1 cKO at P5 and 8w were analyzed for synapse markers. Staining photos are shown for P5 sections. Signal intensities within the rI7 glomeruli were shown. Scale bars=25 μm. * p < 0.05, ** p < 0.01 (Student’s t -test). Error bars indicate SD ( n = 5, 5, 2, 3 animals). d Dendrite morphology in the PlxnC1 cKO. M/T cells (yellow) were visualized by LY injection into the rI7 glomeruli of the WT and PlxnC1 cKO at P5 (left). The ratios (%) of mature (blue) and immature (white) M/T cells are shown (right): WT, 24/31 (77.4 %); KO, 7/31 (22.6 %). n = 23 glomeruli. Scale bar=20 μm
Techniques Used: Staining, Immunostaining, Injection

Figure Legend Snippet: Sema7A promotes dendrite maturation and synapse formation. Left, Sema7A levels in the MOR29B and MOR29A glomeruli. OB sections at P4 were immunostained with anti-Sema7A antibodies, and signal intensities of Sema7A are compared (see also Fig. ). * p < 0.05 (Student’s t -test). Error bars indicate standard deviation SD ( n = 8 glomeruli). Middle, Pre- and post-synaptic markers in the MOR29A and MOR29B glomeruli. OB sections at P4 were also immunostained with antibodies against vGlut2 and GluR1, pre- and post-synaptic markers, respectively. Relative fluorescent signals (MOR29B/MOR29A glomeruli) are compared. * p < 0.05 (Student’s t -test). Error bars indicate standard deviation SD ( n = 8 glomeruli). Right, Dendrite maturation of M/T cells in the MOR29A and MOR29B glomeruli. M/T-cell dendrites were visualized by LY injection into the MOR29A and MOR29B glomeruli at P4. The ratios (%) of mature and immature M/T cells in the MOR29A and MOR29B glomeruli are shown: MOR29A, 8/29 (27.6 %); MOR29B, 22/28 (78.6%). n = 16 glomeruli
Techniques Used: Standard Deviation, Injection
vglut2 (Alomone Labs)


Structured Review

Vglut2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vglut2/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Optogenetic stimulation of Glutamatergic Neuronal Activity in the Striatum Enhances Neurogenesis in the Subventricular Zone of Normal and Stroke Mice"
Article Title: Optogenetic stimulation of Glutamatergic Neuronal Activity in the Striatum Enhances Neurogenesis in the Subventricular Zone of Normal and Stroke Mice
Journal: Neurobiology of disease
doi: 10.1016/j.nbd.2016.11.005

Figure Legend Snippet: A. Confocal imaging of immunostaining was performed in brain sections from wild type C57/B6L mice. The overlapped neurofilament (NF) and VGLUT2 markers and their co-expression with DAPI identified a glutamatergic neuron in the striatum. B and C. Sagittal brain striatum sections show VGLUT2 and NF expressing axons extending to the adjacent SVZ. Images in C show the box area in panel B. The enlarged images show co-localization of VGLUT2 and neurofilament (NF) in the striatum (arrow head), and extension of these nerve fibers toward the SVZ. D. Immunostaining for DCX, the glutamatergic marker VGLUT1 and the presynapse marker Synapsin-1 in the SVZ, and striatum. The merged image revealed dense distribution of the pre-synaptic protein Synapsin-1 in the striatum/SVZ bordering area. The enlarged image from the box area illustrates a few possible synapses co-labeled with Synapsin-1 and DCX+ cells (arrows); most of Synapsin-1 staining, however, located in close proximity of blue colored neuroblasts. E. Axon tracker DiI imaging 14 days after injection into the striatum. The DiI (red) distribution (arrows) due to axonal trafficking was seen along the SVZ and the border region between SVZ and striatum. Representative of brain sections from 5 WT animals.
Techniques Used: Imaging, Immunostaining, Expressing, Marker, Labeling, Staining, Injection

Figure Legend Snippet: A. Immunofluorescence images from brain sections show expression of ChR2-YFP (green) in the cortex and striatum of the ChR2 transgenic mouse. The characteristic patch-matrix structure was seen in the striatum. B to D. In brain sections from ChR2-YFP transgenic mice, immunofluorescence staining shows co-localization of ChR2-YFP with the excitatory glutamatergic neuron marker VGLUT1 (B), VGLUT2 (C) and CaMKIIα (D), demonstrating that ChR2-YFP-expressing neurons (arrows) and fibers have glutamatergic phenotype residing within the cortex and striatum.
Techniques Used: Immunofluorescence, Expressing, Transgenic Assay, Staining, Marker
vglut2 (Alomone Labs)


Structured Review

Vglut2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vglut2/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Optogenetic stimulation of Glutamatergic Neuronal Activity in the Striatum Enhances Neurogenesis in the Subventricular Zone of Normal and Stroke Mice"
Article Title: Optogenetic stimulation of Glutamatergic Neuronal Activity in the Striatum Enhances Neurogenesis in the Subventricular Zone of Normal and Stroke Mice
Journal: Neurobiology of disease
doi: 10.1016/j.nbd.2016.11.005

Figure Legend Snippet: A. Confocal imaging of immunostaining was performed in brain sections from wild type C57/B6L mice. The overlapped neurofilament (NF) and VGLUT2 markers and their co-expression with DAPI identified a glutamatergic neuron in the striatum. B and C. Sagittal brain striatum sections show VGLUT2 and NF expressing axons extending to the adjacent SVZ. Images in C show the box area in panel B. The enlarged images show co-localization of VGLUT2 and neurofilament (NF) in the striatum (arrow head), and extension of these nerve fibers toward the SVZ. D. Immunostaining for DCX, the glutamatergic marker VGLUT1 and the presynapse marker Synapsin-1 in the SVZ, and striatum. The merged image revealed dense distribution of the pre-synaptic protein Synapsin-1 in the striatum/SVZ bordering area. The enlarged image from the box area illustrates a few possible synapses co-labeled with Synapsin-1 and DCX+ cells (arrows); most of Synapsin-1 staining, however, located in close proximity of blue colored neuroblasts. E. Axon tracker DiI imaging 14 days after injection into the striatum. The DiI (red) distribution (arrows) due to axonal trafficking was seen along the SVZ and the border region between SVZ and striatum. Representative of brain sections from 5 WT animals.
Techniques Used: Imaging, Immunostaining, Expressing, Marker, Labeling, Staining, Injection

Figure Legend Snippet: A. Immunofluorescence images from brain sections show expression of ChR2-YFP (green) in the cortex and striatum of the ChR2 transgenic mouse. The characteristic patch-matrix structure was seen in the striatum. B to D. In brain sections from ChR2-YFP transgenic mice, immunofluorescence staining shows co-localization of ChR2-YFP with the excitatory glutamatergic neuron marker VGLUT1 (B), VGLUT2 (C) and CaMKIIα (D), demonstrating that ChR2-YFP-expressing neurons (arrows) and fibers have glutamatergic phenotype residing within the cortex and striatum.
Techniques Used: Immunofluorescence, Expressing, Transgenic Assay, Staining, Marker
polyclonal guinea pig antibody (Alomone Labs)


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Polyclonal Guinea Pig Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal guinea pig antibody/product/Alomone Labs
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
polyclonal guinea pig antibody (Alomone Labs)


Structured Review
Polyclonal Guinea Pig Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal guinea pig antibody/product/Alomone Labs
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99