vglut1  (Alomone Labs)


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    Alomone Labs vglut1
    Morphological analysis of the neuronal and glial components in Cdc42ep4 fl/fl and Cdc42ep4 −/− cerebellar cortices. ( a ) Double-label IF of WT and KO cerebellar cortices for a Purkinje cell marker Car8 (red) and a parallel fibre (that is, granule cell) marker <t>VGluT1</t> (top, green) or a climbing fibre marker VGluT2 (bottom, green). No obvious morphological anomaly, including aberrant CF–PC innervation, was found in the major neuronal components of KO-derived samples. Scale bar, 20 μm. ( b ) Transmission electron microscopy images of WT and KO molecular layers. No obvious ultrastructural difference was found between the genotypes. PF, parallel fibre terminal or bouton. PC, dendritic spine of Purkinje cell. Bergmann glial processes are tinted. Scale bar, 200 nm. ( c ) Cumulative histogram of PSD length of the PF–PC synapses, showing no significant difference between the genotypes ( n =92 synapses from two littermates for each genotype, NS, P > 0.05 by Kolmogorov–Smirnov test). ( d ) Quantitative immunoblot of WT and KO cerebellar PSD fractions for GluA1, GluA2 and GluA4 (the major subunits of the AMPARs), each normalized with PSD-95. There was no significant quantitative difference by genotype ( n =3, NS, P > 0.05 by t -test).
    Vglut1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "A CDC42EP4/septin-based perisynaptic glial scaffold facilitates glutamate clearance"

    Article Title: A CDC42EP4/septin-based perisynaptic glial scaffold facilitates glutamate clearance

    Journal: Nature Communications

    doi: 10.1038/ncomms10090

    Morphological analysis of the neuronal and glial components in Cdc42ep4 fl/fl and Cdc42ep4 −/− cerebellar cortices. ( a ) Double-label IF of WT and KO cerebellar cortices for a Purkinje cell marker Car8 (red) and a parallel fibre (that is, granule cell) marker VGluT1 (top, green) or a climbing fibre marker VGluT2 (bottom, green). No obvious morphological anomaly, including aberrant CF–PC innervation, was found in the major neuronal components of KO-derived samples. Scale bar, 20 μm. ( b ) Transmission electron microscopy images of WT and KO molecular layers. No obvious ultrastructural difference was found between the genotypes. PF, parallel fibre terminal or bouton. PC, dendritic spine of Purkinje cell. Bergmann glial processes are tinted. Scale bar, 200 nm. ( c ) Cumulative histogram of PSD length of the PF–PC synapses, showing no significant difference between the genotypes ( n =92 synapses from two littermates for each genotype, NS, P > 0.05 by Kolmogorov–Smirnov test). ( d ) Quantitative immunoblot of WT and KO cerebellar PSD fractions for GluA1, GluA2 and GluA4 (the major subunits of the AMPARs), each normalized with PSD-95. There was no significant quantitative difference by genotype ( n =3, NS, P > 0.05 by t -test).
    Figure Legend Snippet: Morphological analysis of the neuronal and glial components in Cdc42ep4 fl/fl and Cdc42ep4 −/− cerebellar cortices. ( a ) Double-label IF of WT and KO cerebellar cortices for a Purkinje cell marker Car8 (red) and a parallel fibre (that is, granule cell) marker VGluT1 (top, green) or a climbing fibre marker VGluT2 (bottom, green). No obvious morphological anomaly, including aberrant CF–PC innervation, was found in the major neuronal components of KO-derived samples. Scale bar, 20 μm. ( b ) Transmission electron microscopy images of WT and KO molecular layers. No obvious ultrastructural difference was found between the genotypes. PF, parallel fibre terminal or bouton. PC, dendritic spine of Purkinje cell. Bergmann glial processes are tinted. Scale bar, 200 nm. ( c ) Cumulative histogram of PSD length of the PF–PC synapses, showing no significant difference between the genotypes ( n =92 synapses from two littermates for each genotype, NS, P > 0.05 by Kolmogorov–Smirnov test). ( d ) Quantitative immunoblot of WT and KO cerebellar PSD fractions for GluA1, GluA2 and GluA4 (the major subunits of the AMPARs), each normalized with PSD-95. There was no significant quantitative difference by genotype ( n =3, NS, P > 0.05 by t -test).

    Techniques Used: Marker, Derivative Assay, Transmission Assay, Electron Microscopy

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    Alomone Labs vglut1 antibody
    Expression of NMDA receptors (GluN1) and apposed puncta expressing <t>VGLUT1</t> and GAD67 on DCX immunoreactive cells. (A) Confocal microphotographs of the temporal cortex showing the expression of GluN1 in small type I (A1) and larger type I DCX + cells (A2) . (B) DCX immunoreactive type II cell (green) in the occipital cortex layer II showing puncta expressing the excitatory marker VGLUT1 (red) apposed to its soma and dendrite (arrows). (C) DCX immunoreactive type II cell (green) in the temporal cortex layer II. Note the presence of GAD67 expressing puncta (red) apposed to its soma and dendrite (arrows). (A1) Is a single confocal plane, (A2,B,C) are 2D projections of 4 (A2) 9 (B) and 12 (C) consecutive confocal stacks (0.38 μm apart). Scale bar: 10 μm for (A,B4,B5,C4,C5) ; 40 μm for (B1–3,C1–3) . All confocal images in this figure were from neurosurgical samples.
    Vglut1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vglut1 antibody/product/Alomone Labs
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    Expression of NMDA receptors (GluN1) and apposed puncta expressing VGLUT1 and GAD67 on DCX immunoreactive cells. (A) Confocal microphotographs of the temporal cortex showing the expression of GluN1 in small type I (A1) and larger type I DCX + cells (A2) . (B) DCX immunoreactive type II cell (green) in the occipital cortex layer II showing puncta expressing the excitatory marker VGLUT1 (red) apposed to its soma and dendrite (arrows). (C) DCX immunoreactive type II cell (green) in the temporal cortex layer II. Note the presence of GAD67 expressing puncta (red) apposed to its soma and dendrite (arrows). (A1) Is a single confocal plane, (A2,B,C) are 2D projections of 4 (A2) 9 (B) and 12 (C) consecutive confocal stacks (0.38 μm apart). Scale bar: 10 μm for (A,B4,B5,C4,C5) ; 40 μm for (B1–3,C1–3) . All confocal images in this figure were from neurosurgical samples.

    Journal: Frontiers in Neuroanatomy

    Article Title: Phenotype and Distribution of Immature Neurons in the Human Cerebral Cortex Layer II

    doi: 10.3389/fnana.2022.851432

    Figure Lengend Snippet: Expression of NMDA receptors (GluN1) and apposed puncta expressing VGLUT1 and GAD67 on DCX immunoreactive cells. (A) Confocal microphotographs of the temporal cortex showing the expression of GluN1 in small type I (A1) and larger type I DCX + cells (A2) . (B) DCX immunoreactive type II cell (green) in the occipital cortex layer II showing puncta expressing the excitatory marker VGLUT1 (red) apposed to its soma and dendrite (arrows). (C) DCX immunoreactive type II cell (green) in the temporal cortex layer II. Note the presence of GAD67 expressing puncta (red) apposed to its soma and dendrite (arrows). (A1) Is a single confocal plane, (A2,B,C) are 2D projections of 4 (A2) 9 (B) and 12 (C) consecutive confocal stacks (0.38 μm apart). Scale bar: 10 μm for (A,B4,B5,C4,C5) ; 40 μm for (B1–3,C1–3) . All confocal images in this figure were from neurosurgical samples.

    Article Snippet: Moreover, pre-absorption of VGLUT1 antibody with immunogen peptide eliminates all immunostaining (manufacturer’s product information).

    Techniques: Expressing, Marker

    Morphological analysis of the neuronal and glial components in Cdc42ep4 fl/fl and Cdc42ep4 −/− cerebellar cortices. ( a ) Double-label IF of WT and KO cerebellar cortices for a Purkinje cell marker Car8 (red) and a parallel fibre (that is, granule cell) marker VGluT1 (top, green) or a climbing fibre marker VGluT2 (bottom, green). No obvious morphological anomaly, including aberrant CF–PC innervation, was found in the major neuronal components of KO-derived samples. Scale bar, 20 μm. ( b ) Transmission electron microscopy images of WT and KO molecular layers. No obvious ultrastructural difference was found between the genotypes. PF, parallel fibre terminal or bouton. PC, dendritic spine of Purkinje cell. Bergmann glial processes are tinted. Scale bar, 200 nm. ( c ) Cumulative histogram of PSD length of the PF–PC synapses, showing no significant difference between the genotypes ( n =92 synapses from two littermates for each genotype, NS, P > 0.05 by Kolmogorov–Smirnov test). ( d ) Quantitative immunoblot of WT and KO cerebellar PSD fractions for GluA1, GluA2 and GluA4 (the major subunits of the AMPARs), each normalized with PSD-95. There was no significant quantitative difference by genotype ( n =3, NS, P > 0.05 by t -test).

    Journal: Nature Communications

    Article Title: A CDC42EP4/septin-based perisynaptic glial scaffold facilitates glutamate clearance

    doi: 10.1038/ncomms10090

    Figure Lengend Snippet: Morphological analysis of the neuronal and glial components in Cdc42ep4 fl/fl and Cdc42ep4 −/− cerebellar cortices. ( a ) Double-label IF of WT and KO cerebellar cortices for a Purkinje cell marker Car8 (red) and a parallel fibre (that is, granule cell) marker VGluT1 (top, green) or a climbing fibre marker VGluT2 (bottom, green). No obvious morphological anomaly, including aberrant CF–PC innervation, was found in the major neuronal components of KO-derived samples. Scale bar, 20 μm. ( b ) Transmission electron microscopy images of WT and KO molecular layers. No obvious ultrastructural difference was found between the genotypes. PF, parallel fibre terminal or bouton. PC, dendritic spine of Purkinje cell. Bergmann glial processes are tinted. Scale bar, 200 nm. ( c ) Cumulative histogram of PSD length of the PF–PC synapses, showing no significant difference between the genotypes ( n =92 synapses from two littermates for each genotype, NS, P > 0.05 by Kolmogorov–Smirnov test). ( d ) Quantitative immunoblot of WT and KO cerebellar PSD fractions for GluA1, GluA2 and GluA4 (the major subunits of the AMPARs), each normalized with PSD-95. There was no significant quantitative difference by genotype ( n =3, NS, P > 0.05 by t -test).

    Article Snippet: We used antibodies for septins, SEPT2 (1:2,000), SEPT4 (1:3,000) and SEPT7 (1:4,000) as previously described , GLAST , 3-phosphoglycerate dehydrogenase (Phgdh) , calbindin , carbonic anhydrase 8 (Car8) , VGluT1, 2 (ref. ) and commercial antibodies for GluR1, 2 (Alomone Labs, AGC-004, 1:200, AGC-005, 1:150), GLAST (Frontier Institute, Rb-Af660, 1:2,000), PSD-95 (Cell Signaling, 3450, 1:1,000), CDC42 (Santa Cruz, L0809, 1:100), β-actin (Sigma, A5441, 1:5,000) and α-Tubulin (Sigma, T9026, 1:10,000).

    Techniques: Marker, Derivative Assay, Transmission Assay, Electron Microscopy