Journal: Brain structure & function

Article Title: Axotomy of tributaries of the pelvic and pudendal nerves induces changes in the neurochemistry of mouse dorsal root ganglion neurons and the spinal cord

doi: 10.1007/s00429-015-1019-6

Figure Lengend Snippet: Table 1

Article Snippet: Antibodies included rabbit anti-ATF3 (1:4000; sc-188, Santa Cruz, Dallas, TX, USA), anti-CGRP (1:12,000; C8198, Sigma, Saint Louis, MO, USA), anti-TRPV1 (1:4000; ACC-030, Alomone Labs, Jerusalem, Israel), anti-TH (1:4000; AB152, Millipore, Temecula, CA, USA), anti-VGLUT1 (1:4000; Brumovsky et al. 2007 , 2011a , b; Kawamura et al. 2006 ) or guinea pig anti-VGLUT2 (1:4000; Brumovsky et al. 2007 , 2011a , b; Miyazaki et al. 2003 ).

Techniques: Expressing

Journal: Nature Communications

Article Title: A CDC42EP4/septin-based perisynaptic glial scaffold facilitates glutamate clearance

doi: 10.1038/ncomms10090

Figure Lengend Snippet: ( a ) Double-label IF of WT and KO cerebellar cortices for a Purkinje cell marker Car8 (red) and a parallel fibre (that is, granule cell) marker VGluT1 (top, green) or a climbing fibre marker VGluT2 (bottom, green). No obvious morphological anomaly, including aberrant CF–PC innervation, was found in the major neuronal components of KO-derived samples. Scale bar, 20 μm. ( b ) Transmission electron microscopy images of WT and KO molecular layers. No obvious ultrastructural difference was found between the genotypes. PF, parallel fibre terminal or bouton. PC, dendritic spine of Purkinje cell. Bergmann glial processes are tinted. Scale bar, 200 nm. ( c ) Cumulative histogram of PSD length of the PF–PC synapses, showing no significant difference between the genotypes ( n =92 synapses from two littermates for each genotype, NS, P >0.05 by Kolmogorov–Smirnov test). ( d ) Quantitative immunoblot of WT and KO cerebellar PSD fractions for GluA1, GluA2 and GluA4 (the major subunits of the AMPARs), each normalized with PSD-95. There was no significant quantitative difference by genotype ( n =3, NS, P >0.05 by t -test).

Article Snippet: We used antibodies for septins, SEPT2 (1:2,000), SEPT4 (1:3,000) and SEPT7 (1:4,000) as previously described , GLAST , 3-phosphoglycerate dehydrogenase (Phgdh) , calbindin , carbonic anhydrase 8 (Car8) , VGluT1, 2 (ref. ) and commercial antibodies for GluR1, 2 (Alomone Labs, AGC-004, 1:200, AGC-005, 1:150), GLAST (Frontier Institute, Rb-Af660, 1:2,000), PSD-95 (Cell Signaling, 3450, 1:1,000), CDC42 (Santa Cruz, L0809, 1:100), β-actin (Sigma, A5441, 1:5,000) and α-Tubulin (Sigma, T9026, 1:10,000).

Techniques: Marker, Derivative Assay, Transmission Assay, Electron Microscopy, Western Blot