rabbit polyclonal anti nr3b antibody  (Alomone Labs)


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    Alomone Labs rabbit polyclonal anti nr3b antibody
    Immunocytochemistry demonstrating nuclear colocalization of NR1-1a and <t>NR3B</t> in melanoma cells. Confocal microscopic analysis demonstrated that NR1-1a (red) shows similar nuclear colocalization in melanoma cells with NR3B (green) as NR1. Nuclei of NHEM showed very weak signals of NR1-1a. NR1-1a was present in the cytoplasmic region of NHEM but unequivocal colocalization with NR3B was not detected at any cellular compartment. As an example, two extra arrows are used in the insert that shows M35/01 cells: the dashed arrow points at an area where the immunofluorescent signal of NR1-1a (red) colocalizes with DAPI (blue), resulting in purple tone whereas the spotted arrow points at an area where NR1-1a colocalizes with DAPI and NR3B (green), resulting in orange colour. Photomicrographs of 1 μm thick optical sections passing through the majority of nuclei in the area of the interest are shown. One representative cell pointed by white arrow with numerous (nuclear) colocalizing signals was selected on each picture and presented in the insert. Scale bar: 20 μm. Positive controls for the secondary <t>antibodies</t> and special controls for the primary antibodies were also performed (See Supplementary Information Figure S8A,B ).
    Rabbit Polyclonal Anti Nr3b Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti nr3b antibody/product/Alomone Labs
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti nr3b antibody - by Bioz Stars, 2022-11
    90/100 stars

    Images

    1) Product Images from "NR1 and NR3B Composed Intranuclear N-methyl-d-aspartate Receptor Complexes in Human Melanoma Cells"

    Article Title: NR1 and NR3B Composed Intranuclear N-methyl-d-aspartate Receptor Complexes in Human Melanoma Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19071929

    Immunocytochemistry demonstrating nuclear colocalization of NR1-1a and NR3B in melanoma cells. Confocal microscopic analysis demonstrated that NR1-1a (red) shows similar nuclear colocalization in melanoma cells with NR3B (green) as NR1. Nuclei of NHEM showed very weak signals of NR1-1a. NR1-1a was present in the cytoplasmic region of NHEM but unequivocal colocalization with NR3B was not detected at any cellular compartment. As an example, two extra arrows are used in the insert that shows M35/01 cells: the dashed arrow points at an area where the immunofluorescent signal of NR1-1a (red) colocalizes with DAPI (blue), resulting in purple tone whereas the spotted arrow points at an area where NR1-1a colocalizes with DAPI and NR3B (green), resulting in orange colour. Photomicrographs of 1 μm thick optical sections passing through the majority of nuclei in the area of the interest are shown. One representative cell pointed by white arrow with numerous (nuclear) colocalizing signals was selected on each picture and presented in the insert. Scale bar: 20 μm. Positive controls for the secondary antibodies and special controls for the primary antibodies were also performed (See Supplementary Information Figure S8A,B ).
    Figure Legend Snippet: Immunocytochemistry demonstrating nuclear colocalization of NR1-1a and NR3B in melanoma cells. Confocal microscopic analysis demonstrated that NR1-1a (red) shows similar nuclear colocalization in melanoma cells with NR3B (green) as NR1. Nuclei of NHEM showed very weak signals of NR1-1a. NR1-1a was present in the cytoplasmic region of NHEM but unequivocal colocalization with NR3B was not detected at any cellular compartment. As an example, two extra arrows are used in the insert that shows M35/01 cells: the dashed arrow points at an area where the immunofluorescent signal of NR1-1a (red) colocalizes with DAPI (blue), resulting in purple tone whereas the spotted arrow points at an area where NR1-1a colocalizes with DAPI and NR3B (green), resulting in orange colour. Photomicrographs of 1 μm thick optical sections passing through the majority of nuclei in the area of the interest are shown. One representative cell pointed by white arrow with numerous (nuclear) colocalizing signals was selected on each picture and presented in the insert. Scale bar: 20 μm. Positive controls for the secondary antibodies and special controls for the primary antibodies were also performed (See Supplementary Information Figure S8A,B ).

    Techniques Used: Immunocytochemistry

    Immunocytochemistry demonstrating colocalization of NR1 and NR3B in the nuclei of melanoma cells, but not in the cell membrane in melanocytes. Confocal microscopic evaluation revealed that NR1 (red) was present in the cytosol of every melanoma cell line, while NR3B (green) showed weaker and less diffuse signals in the cytoplasm. Unambiguous plasma membrane immunofluorescent signals were not visible, but NR1 and NR3B colocalized inside the nuclei of melanoma cells. NR1 and NR3B colocalization was absent or undetected in the nuclei of NHEM. That 1-μm thick optical section was selected for presentation which specifically went through the majority of nuclei to show that all nuclei were immunopositive. One representative cell pointed by white arrow was selected for the inserts at each part of the panel. Scale bar: 20 μm. Positive controls for the secondary antibodies and special controls for the primary antibodies were also performed (see Supplementary Information Figure S8A,B ).
    Figure Legend Snippet: Immunocytochemistry demonstrating colocalization of NR1 and NR3B in the nuclei of melanoma cells, but not in the cell membrane in melanocytes. Confocal microscopic evaluation revealed that NR1 (red) was present in the cytosol of every melanoma cell line, while NR3B (green) showed weaker and less diffuse signals in the cytoplasm. Unambiguous plasma membrane immunofluorescent signals were not visible, but NR1 and NR3B colocalized inside the nuclei of melanoma cells. NR1 and NR3B colocalization was absent or undetected in the nuclei of NHEM. That 1-μm thick optical section was selected for presentation which specifically went through the majority of nuclei to show that all nuclei were immunopositive. One representative cell pointed by white arrow was selected for the inserts at each part of the panel. Scale bar: 20 μm. Positive controls for the secondary antibodies and special controls for the primary antibodies were also performed (see Supplementary Information Figure S8A,B ).

    Techniques Used: Immunocytochemistry

    Western blot analysis gave detailed picture on NMDAR subunit protein expression. NR1-1a and NR2A subunits were expressed in melanocytes, while other subunits were undetectable by western blots ( A ). Fractionated melanoma samples revealed that NR1 and NR3B subunits are expressed in every cellular compartment ( B ). In each melanoma cell line NR2A and NR3A were present in the cytosol and membranes, but in the nucleus ( B ). NR2B was undetectable in each compartment ( B ). Immunoblot signals of NR1-1a were detected in the cytosol and membranes, but more importantly also in the nuclei of melanoma cells ( B ). Controls used in the experiments: MITF (microphthalmia associated transcription factor) for melanocyte differentiation control; GAPDH as an internal control for melanocyte whole cell lysates; actin as internal controls for the comparison of the cellular fractions; TATABP (TATA box binding protein) expression as internal control for nuclear fraction analysis. The positive control for detection of NMDAR subunit protein expression was human brain tissue lysate (see Supplementary Information Figure S3 ). Whole membrane pictures of western blots are presented as Supplementary Figure S4 . Normalization of the relative optical density parameters of the protein subunit expressions to the respective control is shown in a graphical format in the Supplementary Information Figures S5–S7 . (C = cytosolic, M = membrane, N = nuclear fractions of melanoma cells).
    Figure Legend Snippet: Western blot analysis gave detailed picture on NMDAR subunit protein expression. NR1-1a and NR2A subunits were expressed in melanocytes, while other subunits were undetectable by western blots ( A ). Fractionated melanoma samples revealed that NR1 and NR3B subunits are expressed in every cellular compartment ( B ). In each melanoma cell line NR2A and NR3A were present in the cytosol and membranes, but in the nucleus ( B ). NR2B was undetectable in each compartment ( B ). Immunoblot signals of NR1-1a were detected in the cytosol and membranes, but more importantly also in the nuclei of melanoma cells ( B ). Controls used in the experiments: MITF (microphthalmia associated transcription factor) for melanocyte differentiation control; GAPDH as an internal control for melanocyte whole cell lysates; actin as internal controls for the comparison of the cellular fractions; TATABP (TATA box binding protein) expression as internal control for nuclear fraction analysis. The positive control for detection of NMDAR subunit protein expression was human brain tissue lysate (see Supplementary Information Figure S3 ). Whole membrane pictures of western blots are presented as Supplementary Figure S4 . Normalization of the relative optical density parameters of the protein subunit expressions to the respective control is shown in a graphical format in the Supplementary Information Figures S5–S7 . (C = cytosolic, M = membrane, N = nuclear fractions of melanoma cells).

    Techniques Used: Western Blot, Expressing, Binding Assay, Positive Control

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    Alomone Labs rabbit polyclonal anti nr3b antibody
    Immunocytochemistry demonstrating nuclear colocalization of NR1-1a and <t>NR3B</t> in melanoma cells. Confocal microscopic analysis demonstrated that NR1-1a (red) shows similar nuclear colocalization in melanoma cells with NR3B (green) as NR1. Nuclei of NHEM showed very weak signals of NR1-1a. NR1-1a was present in the cytoplasmic region of NHEM but unequivocal colocalization with NR3B was not detected at any cellular compartment. As an example, two extra arrows are used in the insert that shows M35/01 cells: the dashed arrow points at an area where the immunofluorescent signal of NR1-1a (red) colocalizes with DAPI (blue), resulting in purple tone whereas the spotted arrow points at an area where NR1-1a colocalizes with DAPI and NR3B (green), resulting in orange colour. Photomicrographs of 1 μm thick optical sections passing through the majority of nuclei in the area of the interest are shown. One representative cell pointed by white arrow with numerous (nuclear) colocalizing signals was selected on each picture and presented in the insert. Scale bar: 20 μm. Positive controls for the secondary <t>antibodies</t> and special controls for the primary antibodies were also performed (See Supplementary Information Figure S8A,B ).
    Rabbit Polyclonal Anti Nr3b Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti nr3b antibody/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti nr3b antibody - by Bioz Stars, 2022-11
    90/100 stars
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    Immunocytochemistry demonstrating nuclear colocalization of NR1-1a and NR3B in melanoma cells. Confocal microscopic analysis demonstrated that NR1-1a (red) shows similar nuclear colocalization in melanoma cells with NR3B (green) as NR1. Nuclei of NHEM showed very weak signals of NR1-1a. NR1-1a was present in the cytoplasmic region of NHEM but unequivocal colocalization with NR3B was not detected at any cellular compartment. As an example, two extra arrows are used in the insert that shows M35/01 cells: the dashed arrow points at an area where the immunofluorescent signal of NR1-1a (red) colocalizes with DAPI (blue), resulting in purple tone whereas the spotted arrow points at an area where NR1-1a colocalizes with DAPI and NR3B (green), resulting in orange colour. Photomicrographs of 1 μm thick optical sections passing through the majority of nuclei in the area of the interest are shown. One representative cell pointed by white arrow with numerous (nuclear) colocalizing signals was selected on each picture and presented in the insert. Scale bar: 20 μm. Positive controls for the secondary antibodies and special controls for the primary antibodies were also performed (See Supplementary Information Figure S8A,B ).

    Journal: International Journal of Molecular Sciences

    Article Title: NR1 and NR3B Composed Intranuclear N-methyl-d-aspartate Receptor Complexes in Human Melanoma Cells

    doi: 10.3390/ijms19071929

    Figure Lengend Snippet: Immunocytochemistry demonstrating nuclear colocalization of NR1-1a and NR3B in melanoma cells. Confocal microscopic analysis demonstrated that NR1-1a (red) shows similar nuclear colocalization in melanoma cells with NR3B (green) as NR1. Nuclei of NHEM showed very weak signals of NR1-1a. NR1-1a was present in the cytoplasmic region of NHEM but unequivocal colocalization with NR3B was not detected at any cellular compartment. As an example, two extra arrows are used in the insert that shows M35/01 cells: the dashed arrow points at an area where the immunofluorescent signal of NR1-1a (red) colocalizes with DAPI (blue), resulting in purple tone whereas the spotted arrow points at an area where NR1-1a colocalizes with DAPI and NR3B (green), resulting in orange colour. Photomicrographs of 1 μm thick optical sections passing through the majority of nuclei in the area of the interest are shown. One representative cell pointed by white arrow with numerous (nuclear) colocalizing signals was selected on each picture and presented in the insert. Scale bar: 20 μm. Positive controls for the secondary antibodies and special controls for the primary antibodies were also performed (See Supplementary Information Figure S8A,B ).

    Article Snippet: After that samples were washed again three times in PBS, and cultures were incubated with rabbit polyclonal anti-NR3B antibody (Alomone Labs, Jerusalem, Israel), at a dilution of 1:50 in PBST, at 4 °C overnight.

    Techniques: Immunocytochemistry

    Immunocytochemistry demonstrating colocalization of NR1 and NR3B in the nuclei of melanoma cells, but not in the cell membrane in melanocytes. Confocal microscopic evaluation revealed that NR1 (red) was present in the cytosol of every melanoma cell line, while NR3B (green) showed weaker and less diffuse signals in the cytoplasm. Unambiguous plasma membrane immunofluorescent signals were not visible, but NR1 and NR3B colocalized inside the nuclei of melanoma cells. NR1 and NR3B colocalization was absent or undetected in the nuclei of NHEM. That 1-μm thick optical section was selected for presentation which specifically went through the majority of nuclei to show that all nuclei were immunopositive. One representative cell pointed by white arrow was selected for the inserts at each part of the panel. Scale bar: 20 μm. Positive controls for the secondary antibodies and special controls for the primary antibodies were also performed (see Supplementary Information Figure S8A,B ).

    Journal: International Journal of Molecular Sciences

    Article Title: NR1 and NR3B Composed Intranuclear N-methyl-d-aspartate Receptor Complexes in Human Melanoma Cells

    doi: 10.3390/ijms19071929

    Figure Lengend Snippet: Immunocytochemistry demonstrating colocalization of NR1 and NR3B in the nuclei of melanoma cells, but not in the cell membrane in melanocytes. Confocal microscopic evaluation revealed that NR1 (red) was present in the cytosol of every melanoma cell line, while NR3B (green) showed weaker and less diffuse signals in the cytoplasm. Unambiguous plasma membrane immunofluorescent signals were not visible, but NR1 and NR3B colocalized inside the nuclei of melanoma cells. NR1 and NR3B colocalization was absent or undetected in the nuclei of NHEM. That 1-μm thick optical section was selected for presentation which specifically went through the majority of nuclei to show that all nuclei were immunopositive. One representative cell pointed by white arrow was selected for the inserts at each part of the panel. Scale bar: 20 μm. Positive controls for the secondary antibodies and special controls for the primary antibodies were also performed (see Supplementary Information Figure S8A,B ).

    Article Snippet: After that samples were washed again three times in PBS, and cultures were incubated with rabbit polyclonal anti-NR3B antibody (Alomone Labs, Jerusalem, Israel), at a dilution of 1:50 in PBST, at 4 °C overnight.

    Techniques: Immunocytochemistry

    Western blot analysis gave detailed picture on NMDAR subunit protein expression. NR1-1a and NR2A subunits were expressed in melanocytes, while other subunits were undetectable by western blots ( A ). Fractionated melanoma samples revealed that NR1 and NR3B subunits are expressed in every cellular compartment ( B ). In each melanoma cell line NR2A and NR3A were present in the cytosol and membranes, but in the nucleus ( B ). NR2B was undetectable in each compartment ( B ). Immunoblot signals of NR1-1a were detected in the cytosol and membranes, but more importantly also in the nuclei of melanoma cells ( B ). Controls used in the experiments: MITF (microphthalmia associated transcription factor) for melanocyte differentiation control; GAPDH as an internal control for melanocyte whole cell lysates; actin as internal controls for the comparison of the cellular fractions; TATABP (TATA box binding protein) expression as internal control for nuclear fraction analysis. The positive control for detection of NMDAR subunit protein expression was human brain tissue lysate (see Supplementary Information Figure S3 ). Whole membrane pictures of western blots are presented as Supplementary Figure S4 . Normalization of the relative optical density parameters of the protein subunit expressions to the respective control is shown in a graphical format in the Supplementary Information Figures S5–S7 . (C = cytosolic, M = membrane, N = nuclear fractions of melanoma cells).

    Journal: International Journal of Molecular Sciences

    Article Title: NR1 and NR3B Composed Intranuclear N-methyl-d-aspartate Receptor Complexes in Human Melanoma Cells

    doi: 10.3390/ijms19071929

    Figure Lengend Snippet: Western blot analysis gave detailed picture on NMDAR subunit protein expression. NR1-1a and NR2A subunits were expressed in melanocytes, while other subunits were undetectable by western blots ( A ). Fractionated melanoma samples revealed that NR1 and NR3B subunits are expressed in every cellular compartment ( B ). In each melanoma cell line NR2A and NR3A were present in the cytosol and membranes, but in the nucleus ( B ). NR2B was undetectable in each compartment ( B ). Immunoblot signals of NR1-1a were detected in the cytosol and membranes, but more importantly also in the nuclei of melanoma cells ( B ). Controls used in the experiments: MITF (microphthalmia associated transcription factor) for melanocyte differentiation control; GAPDH as an internal control for melanocyte whole cell lysates; actin as internal controls for the comparison of the cellular fractions; TATABP (TATA box binding protein) expression as internal control for nuclear fraction analysis. The positive control for detection of NMDAR subunit protein expression was human brain tissue lysate (see Supplementary Information Figure S3 ). Whole membrane pictures of western blots are presented as Supplementary Figure S4 . Normalization of the relative optical density parameters of the protein subunit expressions to the respective control is shown in a graphical format in the Supplementary Information Figures S5–S7 . (C = cytosolic, M = membrane, N = nuclear fractions of melanoma cells).

    Article Snippet: After that samples were washed again three times in PBS, and cultures were incubated with rabbit polyclonal anti-NR3B antibody (Alomone Labs, Jerusalem, Israel), at a dilution of 1:50 in PBST, at 4 °C overnight.

    Techniques: Western Blot, Expressing, Binding Assay, Positive Control