glt1  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    Anti EAAT2 GLT 1 extracellular ATTO Fluor 633 Antibody
    Description:
    Anti EAAT2 GLT 1 extracellular Antibody AGC 022 is a highly specific antibody directed against an epitope of the rat Excitatory amino acid transporter 2 The antibody can be used in western blot live cell imaging and immunohistochemistry applications It has been designed to recognize EAAT2 from human rat and mouse samples nAnti EAAT2 GLT 1 extracellular ATTO Fluor 633 Antibody AGC 022 FR is directly labeled with an ATTO 633 fluorescent dye ATTO dyes are characterized by strong absorption high extinction coefficient high fluorescence quantum yield and high photo stability ATTO 633 has a maximum absorption at 629 nm and a maximum fluorescence at 657 nm The fluorescence is excited most efficiently in the range 610 to 645 nm This label is analogous to the well known dyes Alexa 647 Alexa 633 and Cy5 Anti EAAT2 GLT 1 extracellular ATTO Fluor 633 Antibody has been tested in immunohistochemical staining and is specially suited for experiments requiring simultaneous labeling of different markers
    Catalog Number:
    AGC-022-FR
    Price:
    686.0
    Category:
    Primary Antibody
    Applications:
    Immunofluorescence, Immunohistochemistry
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    50 mcl
    Antibody Type:
    Polyclonal ATTO 633 (Red) Conjugated Primary Antibody
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    Buy from Supplier


    Structured Review

    Alomone Labs glt1
    Anti EAAT2 GLT 1 extracellular ATTO Fluor 633 Antibody
    Anti EAAT2 GLT 1 extracellular Antibody AGC 022 is a highly specific antibody directed against an epitope of the rat Excitatory amino acid transporter 2 The antibody can be used in western blot live cell imaging and immunohistochemistry applications It has been designed to recognize EAAT2 from human rat and mouse samples nAnti EAAT2 GLT 1 extracellular ATTO Fluor 633 Antibody AGC 022 FR is directly labeled with an ATTO 633 fluorescent dye ATTO dyes are characterized by strong absorption high extinction coefficient high fluorescence quantum yield and high photo stability ATTO 633 has a maximum absorption at 629 nm and a maximum fluorescence at 657 nm The fluorescence is excited most efficiently in the range 610 to 645 nm This label is analogous to the well known dyes Alexa 647 Alexa 633 and Cy5 Anti EAAT2 GLT 1 extracellular ATTO Fluor 633 Antibody has been tested in immunohistochemical staining and is specially suited for experiments requiring simultaneous labeling of different markers
    https://www.bioz.com/result/glt1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glt1 - by Bioz Stars, 2021-09
    93/100 stars

    Images

    1) Product Images from "Deletion of the Clock Gene Period2 (Per2) in Glial Cells Alters Mood-Related Behavior in Mice"

    Article Title: Deletion of the Clock Gene Period2 (Per2) in Glial Cells Alters Mood-Related Behavior in Mice

    Journal: bioRxiv

    doi: 10.1101/2020.12.09.417162

    Adeno-associated virus (AAV)-mediated deletion of Per2 in glial cells of adult mice leads to a depression resistant behavior. (A) Fluorescent imaging of whole brains 3 weeks after no injection (left), intravenous (i.v.) injection of the engineered AAV-PHP.eB, which can pass the blood-brain barrier (BBB), containing the general CAG driver (second from left) or the glial Gfap driver (middle). The second to last brain is from an animal with i.v. injected AAV-9, which does not pass the BBB, containing the general CAG driver. The last brain (right) is from an animal injected intraperitoneally (i.p.) with the AAV-PHP.eB Gfap -driven construct. Note that only the brains of animals that received the AAV-PHP.eb i.v. display significant fluorescent signal after 3 weeks (orange and yellow color). (B) Fluorescent imaging of whole brains 2 months after injection of the AAV-PHP.eB. Note that the fluorescence is still maintained after 2 months post injection and that even the i.p. injected AAV-PHP.eB Gfap is showing signal in the brain now. (C) Sorting of neurons and astrocytes by flow cytometry from brain tissue including the nucleus accumbens (NAc). The left panel shows the removal of debris from a single cell suspension, showing the distribution of debris in the forward as well as in the side scatter (FSC and SSC, respectively). The middle panel shows the removal of CD11b + cells (microglia) from the cell suspension (lower left corner from left panel). The CD11b - cells (bottom half from middle panel) were then sorted into two distinct cell populations corresponding to astrocytes (GLT1 + /CD90.2 - ) and neurons (CD90.2 + /GLT1 - ) (right panel). (D) PCR analysis of astrocytes and neurons from the cell sorting. Microglia (CD11 + ) as well as astrocytes (GLT1 + /CD90.2 - ), but not neurons (CD90.2 + /GLT1 - ) from PHP.eB Gfap-iCre infected animals show the presence of iCre , indicating that only glia and not neurons could express iCre in order to delete Per2 in the Per2 fl/fl mice. (E) Immunohistochemistry of vG Per2 brain tissue from nucleus accumbens (NAc) isolated at ZT6. The signal for PER2 (green) mainly overlaps with neuronal NeuN signal (red) giving rise to the yellow color. Scale bar: 100 µm. (F) Immobility time in the forced swim test (FST) of vG Per2 (PHP.eB Gfap-iCre , green) and control (PHP.eB control, blue) animals are shown (n = 5, two-tailed t-test, *p
    Figure Legend Snippet: Adeno-associated virus (AAV)-mediated deletion of Per2 in glial cells of adult mice leads to a depression resistant behavior. (A) Fluorescent imaging of whole brains 3 weeks after no injection (left), intravenous (i.v.) injection of the engineered AAV-PHP.eB, which can pass the blood-brain barrier (BBB), containing the general CAG driver (second from left) or the glial Gfap driver (middle). The second to last brain is from an animal with i.v. injected AAV-9, which does not pass the BBB, containing the general CAG driver. The last brain (right) is from an animal injected intraperitoneally (i.p.) with the AAV-PHP.eB Gfap -driven construct. Note that only the brains of animals that received the AAV-PHP.eb i.v. display significant fluorescent signal after 3 weeks (orange and yellow color). (B) Fluorescent imaging of whole brains 2 months after injection of the AAV-PHP.eB. Note that the fluorescence is still maintained after 2 months post injection and that even the i.p. injected AAV-PHP.eB Gfap is showing signal in the brain now. (C) Sorting of neurons and astrocytes by flow cytometry from brain tissue including the nucleus accumbens (NAc). The left panel shows the removal of debris from a single cell suspension, showing the distribution of debris in the forward as well as in the side scatter (FSC and SSC, respectively). The middle panel shows the removal of CD11b + cells (microglia) from the cell suspension (lower left corner from left panel). The CD11b - cells (bottom half from middle panel) were then sorted into two distinct cell populations corresponding to astrocytes (GLT1 + /CD90.2 - ) and neurons (CD90.2 + /GLT1 - ) (right panel). (D) PCR analysis of astrocytes and neurons from the cell sorting. Microglia (CD11 + ) as well as astrocytes (GLT1 + /CD90.2 - ), but not neurons (CD90.2 + /GLT1 - ) from PHP.eB Gfap-iCre infected animals show the presence of iCre , indicating that only glia and not neurons could express iCre in order to delete Per2 in the Per2 fl/fl mice. (E) Immunohistochemistry of vG Per2 brain tissue from nucleus accumbens (NAc) isolated at ZT6. The signal for PER2 (green) mainly overlaps with neuronal NeuN signal (red) giving rise to the yellow color. Scale bar: 100 µm. (F) Immobility time in the forced swim test (FST) of vG Per2 (PHP.eB Gfap-iCre , green) and control (PHP.eB control, blue) animals are shown (n = 5, two-tailed t-test, *p

    Techniques Used: Mouse Assay, Imaging, Injection, Construct, Fluorescence, Flow Cytometry, Polymerase Chain Reaction, FACS, Infection, Immunohistochemistry, Isolation, Two Tailed Test

    Related Articles

    Staining:

    Article Title: High-Throughput Liquid Chromatography–Tandem Mass Spectrometry Quantification of Glycosaminoglycans as Biomarkers of Mucopolysaccharidosis II
    Article Snippet: .. Cells were Fc blocked (Biolegend #101320, 1:100) and stained for flow cytometric analysis with Fixable Viability Stain BV510 (BD Biosciences #564406, 1:100) to exclude dead cells, CD11b-BV421 (BD Biosciences 562605, 1:100), CD31-PerCP Cy5.5 (BD Biosciences #562861, 1:100), O1-488 (Thermo/eBio #14-6506-82, 1:37.5), Thy1-PE (R & D #FAB7335P, 1:100), and EAAT2-633 (Alomone #AGC-022-FR, 1:50). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Alomone Labs glt1
    Adeno-associated virus (AAV)-mediated deletion of Per2 in glial cells of adult mice leads to a depression resistant behavior. (A) Fluorescent imaging of whole brains 3 weeks after no injection (left), intravenous (i.v.) injection of the engineered AAV-PHP.eB, which can pass the blood-brain barrier (BBB), containing the general CAG driver (second from left) or the glial Gfap driver (middle). The second to last brain is from an animal with i.v. injected AAV-9, which does not pass the BBB, containing the general CAG driver. The last brain (right) is from an animal injected intraperitoneally (i.p.) with the AAV-PHP.eB Gfap -driven construct. Note that only the brains of animals that received the AAV-PHP.eb i.v. display significant fluorescent signal after 3 weeks (orange and yellow color). (B) Fluorescent imaging of whole brains 2 months after injection of the AAV-PHP.eB. Note that the fluorescence is still maintained after 2 months post injection and that even the i.p. injected AAV-PHP.eB Gfap is showing signal in the brain now. (C) Sorting of neurons and astrocytes by flow cytometry from brain tissue including the nucleus accumbens (NAc). The left panel shows the removal of debris from a single cell suspension, showing the distribution of debris in the forward as well as in the side scatter (FSC and SSC, respectively). The middle panel shows the removal of CD11b + cells (microglia) from the cell suspension (lower left corner from left panel). The CD11b - cells (bottom half from middle panel) were then sorted into two distinct cell populations corresponding to astrocytes <t>(GLT1</t> + /CD90.2 - ) and neurons (CD90.2 + /GLT1 - ) (right panel). (D) PCR analysis of astrocytes and neurons from the cell sorting. Microglia (CD11 + ) as well as astrocytes (GLT1 + /CD90.2 - ), but not neurons (CD90.2 + /GLT1 - ) from PHP.eB Gfap-iCre infected animals show the presence of iCre , indicating that only glia and not neurons could express iCre in order to delete Per2 in the Per2 fl/fl mice. (E) Immunohistochemistry of vG Per2 brain tissue from nucleus accumbens (NAc) isolated at ZT6. The signal for PER2 (green) mainly overlaps with neuronal NeuN signal (red) giving rise to the yellow color. Scale bar: 100 µm. (F) Immobility time in the forced swim test (FST) of vG Per2 (PHP.eB Gfap-iCre , green) and control (PHP.eB control, blue) animals are shown (n = 5, two-tailed t-test, *p
    Glt1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glt1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glt1 - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Adeno-associated virus (AAV)-mediated deletion of Per2 in glial cells of adult mice leads to a depression resistant behavior. (A) Fluorescent imaging of whole brains 3 weeks after no injection (left), intravenous (i.v.) injection of the engineered AAV-PHP.eB, which can pass the blood-brain barrier (BBB), containing the general CAG driver (second from left) or the glial Gfap driver (middle). The second to last brain is from an animal with i.v. injected AAV-9, which does not pass the BBB, containing the general CAG driver. The last brain (right) is from an animal injected intraperitoneally (i.p.) with the AAV-PHP.eB Gfap -driven construct. Note that only the brains of animals that received the AAV-PHP.eb i.v. display significant fluorescent signal after 3 weeks (orange and yellow color). (B) Fluorescent imaging of whole brains 2 months after injection of the AAV-PHP.eB. Note that the fluorescence is still maintained after 2 months post injection and that even the i.p. injected AAV-PHP.eB Gfap is showing signal in the brain now. (C) Sorting of neurons and astrocytes by flow cytometry from brain tissue including the nucleus accumbens (NAc). The left panel shows the removal of debris from a single cell suspension, showing the distribution of debris in the forward as well as in the side scatter (FSC and SSC, respectively). The middle panel shows the removal of CD11b + cells (microglia) from the cell suspension (lower left corner from left panel). The CD11b - cells (bottom half from middle panel) were then sorted into two distinct cell populations corresponding to astrocytes (GLT1 + /CD90.2 - ) and neurons (CD90.2 + /GLT1 - ) (right panel). (D) PCR analysis of astrocytes and neurons from the cell sorting. Microglia (CD11 + ) as well as astrocytes (GLT1 + /CD90.2 - ), but not neurons (CD90.2 + /GLT1 - ) from PHP.eB Gfap-iCre infected animals show the presence of iCre , indicating that only glia and not neurons could express iCre in order to delete Per2 in the Per2 fl/fl mice. (E) Immunohistochemistry of vG Per2 brain tissue from nucleus accumbens (NAc) isolated at ZT6. The signal for PER2 (green) mainly overlaps with neuronal NeuN signal (red) giving rise to the yellow color. Scale bar: 100 µm. (F) Immobility time in the forced swim test (FST) of vG Per2 (PHP.eB Gfap-iCre , green) and control (PHP.eB control, blue) animals are shown (n = 5, two-tailed t-test, *p

    Journal: bioRxiv

    Article Title: Deletion of the Clock Gene Period2 (Per2) in Glial Cells Alters Mood-Related Behavior in Mice

    doi: 10.1101/2020.12.09.417162

    Figure Lengend Snippet: Adeno-associated virus (AAV)-mediated deletion of Per2 in glial cells of adult mice leads to a depression resistant behavior. (A) Fluorescent imaging of whole brains 3 weeks after no injection (left), intravenous (i.v.) injection of the engineered AAV-PHP.eB, which can pass the blood-brain barrier (BBB), containing the general CAG driver (second from left) or the glial Gfap driver (middle). The second to last brain is from an animal with i.v. injected AAV-9, which does not pass the BBB, containing the general CAG driver. The last brain (right) is from an animal injected intraperitoneally (i.p.) with the AAV-PHP.eB Gfap -driven construct. Note that only the brains of animals that received the AAV-PHP.eb i.v. display significant fluorescent signal after 3 weeks (orange and yellow color). (B) Fluorescent imaging of whole brains 2 months after injection of the AAV-PHP.eB. Note that the fluorescence is still maintained after 2 months post injection and that even the i.p. injected AAV-PHP.eB Gfap is showing signal in the brain now. (C) Sorting of neurons and astrocytes by flow cytometry from brain tissue including the nucleus accumbens (NAc). The left panel shows the removal of debris from a single cell suspension, showing the distribution of debris in the forward as well as in the side scatter (FSC and SSC, respectively). The middle panel shows the removal of CD11b + cells (microglia) from the cell suspension (lower left corner from left panel). The CD11b - cells (bottom half from middle panel) were then sorted into two distinct cell populations corresponding to astrocytes (GLT1 + /CD90.2 - ) and neurons (CD90.2 + /GLT1 - ) (right panel). (D) PCR analysis of astrocytes and neurons from the cell sorting. Microglia (CD11 + ) as well as astrocytes (GLT1 + /CD90.2 - ), but not neurons (CD90.2 + /GLT1 - ) from PHP.eB Gfap-iCre infected animals show the presence of iCre , indicating that only glia and not neurons could express iCre in order to delete Per2 in the Per2 fl/fl mice. (E) Immunohistochemistry of vG Per2 brain tissue from nucleus accumbens (NAc) isolated at ZT6. The signal for PER2 (green) mainly overlaps with neuronal NeuN signal (red) giving rise to the yellow color. Scale bar: 100 µm. (F) Immobility time in the forced swim test (FST) of vG Per2 (PHP.eB Gfap-iCre , green) and control (PHP.eB control, blue) animals are shown (n = 5, two-tailed t-test, *p

    Article Snippet: Antibodies targeted CD11b (fluorophore PECy7, ref. 552850, BD Pharmingen), CD90.2 (fluorophore PE, ref., 130-102-489, Miltenyi Biotech) and GLT1 (fluorophore ATTO 633, ref., AGC-022-FR, Alomone lab), and were used in a 1:100 dilution.

    Techniques: Mouse Assay, Imaging, Injection, Construct, Fluorescence, Flow Cytometry, Polymerase Chain Reaction, FACS, Infection, Immunohistochemistry, Isolation, Two Tailed Test