Journal: PLoS ONE
Article Title: AMPA Receptor Regulation at the mRNA and Protein Level in Rat Primary Cortical Cultures
Figure Lengend Snippet: AMPA receptor expression is predominant in neuronal cells. Double labeling of rat cortical neurons at DIV5 and DIV26 (20×) with an antibody against the glial marker GFAP (green) and with antibodies against the four AMPA receptor subunits: (A, B) GluR1; (C, D) GluR2; (E, F) GluR3 and (G, H) GluR4 (red). The images were acquired from rare regions with high density of glial cells to show that the GFAP-positive cells do not express AMPA receptors. Scale bar: 50 µm.
Article Snippet: The AMPA receptor subunit analysis was carried out with polyclonal rabbit anti-GluR1 (1∶40; Millipore), anti-GluR2 (1∶200; Millipore), anti-GluR3 (1∶50, Alomone Labs Ltd.), polyclonal goat anti-GluR4 (1∶50; Abcam, Cambridge, UK).
Techniques: Expressing, Labeling, Marker