mglur5  (Alomone Labs)

Journal: Biology of Reproduction

Article Title: Glutamate can act as a signaling molecule in mouse preimplantation embryos †

doi: 10.1093/biolre/ioac126

Figure Lengend Snippet: Transcripts encoding metabotropic glutamate receptors are expressed in mouse blastocysts and oocytes. Metabotropic glutamate receptor types are listed in the tables on the left. In transcripts which were consistently expressed in both blastocysts and oocytes, fold regulation values (“+" means upregulation and “−" means downregulation in blastocysts compared to oocytes) and corresponding P -values are shown. Transcripts were detected by RT-PCR and representative agarose gels with separated PCR products are shown in the panels on the right. Lanes: Grm1 , Glutamate receptor, metabotropic 1; Grm2 , Glutamate receptor, metabotropic 2; Grm3 , Glutamate receptor, metabotropic 3; Grm4 , Glutamate receptor, metabotropic 4; Grm5 , Glutamate receptor, metabotropic 5; Grm6 , Glutamate receptor, metabotropic 6; Grm7 , Glutamate receptor, metabotropic 7; Grm8 , Glutamate receptor, metabotropic 8; MW, molecular weight markers; A, positive control tissue; B, ovulated oocytes; C, blastocysts. The MWs in base pairs (bp) are indicated to the right of the panels. * Primers for Grm5 receptor type were designed in this study.

Article Snippet: Anti-mGluR5 Antibody (Alomone Labs, Cat# AGC-007, dilution 1:50), Anti-GRIK3 (GluK3) Antibody (Alomone Labs, Cat# AGC-040, dilution 1:100), Anti-GRIK4 (KA1) Antibody (Alomone Labs, Cat# AGC-041, dilution 1:50), Anti-GRIK5 (GluK5) Antibody (Alomone Labs, Cat# AGC-042, dilution 1:100), GRIA3 Antibody (LifeSpan Biosciences, Cat# LS-C331307, dilution 1:50), and Anti-GluR4 (GluA4) Antibody (Alomone Labs, Cat# AGC-019, dilution 1:50).

Techniques: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Molecular Weight, Positive Control

Journal: Biology of Reproduction

Article Title: Glutamate can act as a signaling molecule in mouse preimplantation embryos †

doi: 10.1093/biolre/ioac126

Figure Lengend Snippet: Glutamate effects are blocked with AMPA/kainate and GRM5 receptor antagonists. Cell numbers and proportions of dead cells in blastocysts pretreated with the mixture of AMPA, kainate, and GRM5 receptor antagonists (CNQX and MPEP) prior to L-glutamic acid exposure. GlAc 5 mM, blastocysts incubated with L-glutamic acid (at 5 mM final concentration) for 24 h; CNQX + MPEP, blastocysts incubated with CNQX and MPEP (at 300 μM and 10 μM final concentrations, respectively) for 24 h; GlAc 5 mM + (CNQX + MPEP), blastocysts incubated with CNQX + MPEP antagonists for 20 min prior to addition of L-glutamic acid (for the following 24 h incubation). TE, trophectoderm, ICM, inner cell mass. The number of blastocysts in the groups ( n ): Control, n = 32; GlAc 5 mM, n = 33; CNQX + MPEP, n = 28; GlAc 5 mM + (CNQX + MPEP), n = 34. The values are arithmetical mean + SEM. Statistical significance of differences: * P

Article Snippet: Anti-mGluR5 Antibody (Alomone Labs, Cat# AGC-007, dilution 1:50), Anti-GRIK3 (GluK3) Antibody (Alomone Labs, Cat# AGC-040, dilution 1:100), Anti-GRIK4 (KA1) Antibody (Alomone Labs, Cat# AGC-041, dilution 1:50), Anti-GRIK5 (GluK5) Antibody (Alomone Labs, Cat# AGC-042, dilution 1:100), GRIA3 Antibody (LifeSpan Biosciences, Cat# LS-C331307, dilution 1:50), and Anti-GluR4 (GluA4) Antibody (Alomone Labs, Cat# AGC-019, dilution 1:50).

Techniques: Incubation, Concentration Assay