Anti Pan Mglu5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Actinin-4 Governs Dendritic Spine Dynamics and Promotes Their Remodeling by Metabotropic Glutamate Receptors *"
Article Title: Actinin-4 Governs Dendritic Spine Dynamics and Promotes Their Remodeling by Metabotropic Glutamate Receptors *
Journal: The Journal of Biological Chemistry
Figure Legend Snippet: Group 1 mGluRs interact with Actn4. A , schematic of wild type Actn4 and deletion mutants fused to GST used in a pulldown assay with mGlu 1b . B , the Actn4 CH region is necessary and sufficient for mGlu 1b binding. Shown are representative immunoblots ( IB )of a pulldown assay between GST-Actn4 fusion proteins from A and lysates of HEK293 cells transfected with mGlu 1b , probed with anti-mGlu 1b , and stained with Ponceau. C and D , the mGlu 1 cytoplasmic tail is sufficient for Actn4 binding. Shown is a schematic of chimeric constructs of VSVG protein fused to mGlu 1b or mGlu 1a carboxyl tails. Yellow highlights the membrane-proximal region of the tail that is conserved between receptor isoforms (CR region, see below). Also shown are representative immunoblots of input (20 μg) and pulldown (input lysate, 2 mg) between GST-Actn4 and VSVG-mGlu 1a-Tail ( D ) and VSVG-mGlu 1b-Tail ( C ) probed with anti-mGlu 1a or anti-mGlu 1b antibody, respectively. The estimated pulldown fraction in vitro is ∼1% of input receptors. E , Actn4 interacts directly with the CR region of the mGlu 1 intracellular tail. Shown is a schematic of the CR construct and immunoblots illustrating in vitro binding of a purified His-tagged CR fragment and GST-tagged full-length Actn4 ( GST-Actn4 , left panel ) or Actn4 CH region ( GST-Actn4 1–296 , right panel ). Shown are bound and unbound (14% of input GST-tagged proteins) fractions probed with anti-GST and anti-His tag antibodies. The arrow points to full-length GST-Actn4 ( left panel ). Lower molecular mass bands were derived from partial protein fragmentation. The estimated relative bound fractions are 40% and 26% of input for GST-Actn4 and GST-Actn41–296, respectively. F , Actn4 coprecipitates with mGlu 5 in the brain. Shown are representative immunoblots of input lysate and precipitated proteins (from 2.5 mg of input lysate) probed with anti-Actn4 (input, 10 μg) and anti-mGlu 5 (input, 40 μg). The estimated immunoprecipitation (IP) is 5% and 3% of input for mGlu5 and Actn4, respectively.
Techniques Used: Binding Assay, Western Blot, Transfection, Staining, Construct, In Vitro, Purification, Derivative Assay, Immunoprecipitation