Anti Pan Mglu5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Actinin-4 Governs Dendritic Spine Dynamics and Promotes Their Remodeling by Metabotropic Glutamate Receptors *"
Article Title: Actinin-4 Governs Dendritic Spine Dynamics and Promotes Their Remodeling by Metabotropic Glutamate Receptors *
Journal: The Journal of Biological Chemistry
Figure Legend Snippet: Group 1 mGluRs interact with Actn4. A , schematic of wild type Actn4 and deletion mutants fused to GST used in a pulldown assay with mGlu 1b . B , the Actn4 CH region is necessary and sufficient for mGlu 1b binding. Shown are representative immunoblots ( IB )of a pulldown assay between GST-Actn4 fusion proteins from A and lysates of HEK293 cells transfected with mGlu 1b , probed with anti-mGlu 1b , and stained with Ponceau. C and D , the mGlu 1 cytoplasmic tail is sufficient for Actn4 binding. Shown is a schematic of chimeric constructs of VSVG protein fused to mGlu 1b or mGlu 1a carboxyl tails. Yellow highlights the membrane-proximal region of the tail that is conserved between receptor isoforms (CR region, see below). Also shown are representative immunoblots of input (20 μg) and pulldown (input lysate, 2 mg) between GST-Actn4 and VSVG-mGlu 1a-Tail ( D ) and VSVG-mGlu 1b-Tail ( C ) probed with anti-mGlu 1a or anti-mGlu 1b antibody, respectively. The estimated pulldown fraction in vitro is ∼1% of input receptors. E , Actn4 interacts directly with the CR region of the mGlu 1 intracellular tail. Shown is a schematic of the CR construct and immunoblots illustrating in vitro binding of a purified His-tagged CR fragment and GST-tagged full-length Actn4 ( GST-Actn4 , left panel ) or Actn4 CH region ( GST-Actn4 1–296 , right panel ). Shown are bound and unbound (14% of input GST-tagged proteins) fractions probed with anti-GST and anti-His tag antibodies. The arrow points to full-length GST-Actn4 ( left panel ). Lower molecular mass bands were derived from partial protein fragmentation. The estimated relative bound fractions are 40% and 26% of input for GST-Actn4 and GST-Actn41–296, respectively. F , Actn4 coprecipitates with mGlu 5 in the brain. Shown are representative immunoblots of input lysate and precipitated proteins (from 2.5 mg of input lysate) probed with anti-Actn4 (input, 10 μg) and anti-mGlu 5 (input, 40 μg). The estimated immunoprecipitation (IP) is 5% and 3% of input for mGlu5 and Actn4, respectively.
Techniques Used: Binding Assay, Western Blot, Transfection, Staining, Construct, In Vitro, Purification, Derivative Assay, Immunoprecipitation
2) Product Images from "Ablation of SNX6 leads to defects in synaptic function of CA1 pyramidal neurons and spatial memory"
Article Title: Ablation of SNX6 leads to defects in synaptic function of CA1 pyramidal neurons and spatial memory
Figure Legend Snippet: Homer1a overexpression increases surface mGluR5 expression. This experiment serves as a control for Figure 9—figure supplement 2 and is in agreement with previous reports ( Ango et al., 2002 ). ( A ) Hippocampal neurons were co-transfected with pLL3.7.1 and EGFP-Homer1a or EGFP construct on DIV13, and fixed on DIV18. Surface mGluR5 expressions were immunostained with antibodies to mGluR5. ( B ) Quantification of surface mGluR5 signal in ( A ) (mean ± SEM, n = 30 cells, N = 3). Bar: 2 μm. DOI: http://dx.doi.org/10.7554/eLife.20991.035
Techniques Used: Over Expression, Expressing, Transfection, Construct
Figure Legend Snippet: Ablation of SNX6 causes an increase in surface expression of mGluR5 in hippocampal neurons, which is rescued by SNX6 or Homer1c. ( A ) DIV13 neurons were transfected with constructs expressing DsRed and EGFP, EGFP-SNX6, mEmerald-Homer1c-FL or EGFP-Homer1c-C, fixed on DIV18 and immunostained with antibodies to surface-expressed mGluR5. ( B ) Quantification of surface mGluR5 fluorescence intensity per μm 2 (mean ± SEM, n = 30, N = 3, the exact p values are indicated in the bar graph). Bar: 2 μm. DOI: http://dx.doi.org/10.7554/eLife.20991.034
Techniques Used: Expressing, Transfection, Construct, Fluorescence
3) Product Images from "Nanoparticle delivery of CRISPR into the brain rescues a mouse model of fragile X syndrome from exaggerated repetitive behaviours"
Article Title: Nanoparticle delivery of CRISPR into the brain rescues a mouse model of fragile X syndrome from exaggerated repetitive behaviours
Journal: Nature biomedical engineering
Figure Legend Snippet: mGluR5–CRISPR successfully promotes mGluR5 gene editing in the striatum of wild-type and Fmr1 knockout mice. a , Upper: schematic of the injection process for mGluR5–CRISPR into the striatum of wild-type (WT) and Fmr1 knockout (KO) mice. Saline or mGluR5–CRISPR was injected into the striatum (Bregma: 0.26 mm, 3 injection sites per hemisphere are indicated as blue dots, 0.4-mm interval) of WT or Fmr1 KO mice. Lower: schematic of the target sequences of Cas9 RNPs and the protospacer adjacent motif (PAM) for Grm5 knockout. b , RNA was extracted from the saline-injected control side (control) or from the mGluR5–CRISPR-injected side (mGluR5–CRISPR) of WT or Fmr1 KO mice 11 weeks after stereotaxic injections. mRNA levels of Grm5 were amplified and analysed by RT-qPCR. Fold-change of Grm5 mRNA levels are shown after normalization against PPIA mRNA levels. n = 4–6, mean ± s.e.m., *** P
Techniques Used: CRISPR, Knock-Out, Mouse Assay, Injection, Amplification, Quantitative RT-PCR
Figure Legend Snippet: Knocking out mGluR5 using mGluR5–CRISPR significantly rescues the increased repetitive behaviours in Fmr1 knockout mice. a–c , Three weeks after stereotaxic injection of either saline (control) or mGluR5–CRISPR into the striatum of WT and Fmr1 KO mice, the marble-burying assay ( a ) or the empty cage observation test ( b , c ) was performed. a , Left: percentage of marbles buried after 30 min of the marble-burying test. Right: representative images after marble-burying assay for 30 min. Jumping ( b ) and line crossing behaviours ( c ) were scored during 10 min of an empty cage observation test. n = 10–12 for each group, mean ± s.e.m., * P
Techniques Used: CRISPR, Knock-Out, Mouse Assay, Injection