anti mglur1  (Alomone Labs)

Journal: PLoS ONE

Article Title: Disassembly of Shank and Homer Synaptic Clusters Is Driven by Soluble ?-Amyloid1-40 through Divergent NMDAR-Dependent Signalling Pathways

doi: 10.1371/journal.pone.0006011

Figure Lengend Snippet: Dispersal of synaptic mGluR1 clusters by Aβ requires NMDAR and VDCC activity. Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p

Article Snippet: Cells were incubated with anti-mGluR1 (1∶100, Alomone laboratories, Jerusalem, Israel) for 1 h at 25°C and with Alexa-488 conjugated goat anti-rabbit IgG (1∶500).

Techniques: Activity Assay

Journal: The European Journal of Neuroscience

Article Title: Metabotropic glutamate receptors (mGluR5) activate TRPC channels to improve the regularity of the respiratory rhythm generated by the pre-B?tzinger Complex in mice

doi: 10.1111/j.1460-9568.2012.08091.x

Figure Lengend Snippet: Blocking mGluR5 with MPEP increases inspiratory neuron burst irregularity and decreases neuron burst frequency while additionally blocking mGluR1 with LY367385 does not further alter these parameters

Article Snippet: The membranes were blocked overnight at +4°C with 2% non-fat dried milk (NFDM) (Bio-Rad Labs, Hercules, CA, USA) and 2% BSA (Sigma Aldrich, Milwaukee, WI, USA) in Tris buffered saline, pH = 7.5, containing 0.1% Tween-20 (TBS-T) buffer and immuno-blotted for 2h at room temperature with either anti-mGluR5 antibody (1:400) (Abcam, Cambridge, MA, USA), anti-mGluR1 antibody (1:800) (Alomone labs, Jerusalem, Israel), or anti-GAPDH antibody (1:1000, Abcam, USA) in 2% non-fat dried milk in TBS-T buffer.

Techniques: Blocking Assay

Journal: Scientific Reports

Article Title: Metabotropic glutamate receptor-1 regulates inflammation in triple negative breast cancer

doi: 10.1038/s41598-018-34502-8

Figure Lengend Snippet: GRM1 and mGluR1 expression in TNBC. ( A ) Knockdown of GRM1 was accomplished by infecting MDA-MB-231 cells with GIPZ shRNA Lentiviral vectors containing a puromycin resistance gene and shRNA against GRM1 or a non-silencing shRNA construct (NS). GRM1 overexpression was accomplished by infecting MDA-MB-468 cells with pLenti6.3/V5-TOPO vectors containing a blasticidin resistance gene and GRM1 or LACZ construct ( LACZ ). Cells were stably selected with puromycin (1 μg/ml) or blasticidin (5 μg/ml) for 7 days and levels of GRM1 message ( A ) or its corresponding protein, mGluR1 ( B ) were measured by QPCR or Western blot, respectively. mGluR1 expression in SUM159, BT549 and non-transduced MDA-MB-231 TNBC cell lines were also detected. Results in A represent n = 2 experiments and are expressed as the mean ± SEM where *is P

Article Snippet: Immunodetection of mGluR1 was performed using anti-mGluR1 antibody (Alamone Labs, Jerusalum, Israel) with appropriate secondary antibody and detected by chemiluminescence.

Techniques: Expressing, Multiple Displacement Amplification, shRNA, Construct, Over Expression, Stable Transfection, Real-time Polymerase Chain Reaction, Western Blot