anti mglur1  (Alomone Labs)


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    Name:
    Anti mGluR1 extracellular Antibody
    Description:
    Anti mGluR1 extracellular Antibody AGC 006 is a highly specific antibody directed against the extracellular N terminus domain of rat mGluR1 The antibody can be used in western blot and immunocytochemistry applications and recognizes mGluR1 in rat mouse and human samples
    Catalog Number:
    AGC-006
    Price:
    397.0
    Category:
    Primary Antibody
    Applications:
    Immunocytochemistry, Immunofluorescence, Indirect Flow Cytometry, Immunoprecipitation, Live Cell Imaging, Western Blot
    Purity:
    Affinity purified on immobilized antigen.
    Immunogen:
    Synthetic peptide
    Size:
    25 mcl
    Antibody Type:
    Polyclonal Primary Antibodies
    Format:
    Lyophilized Powder
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Alomone Labs anti mglur1
    Anti mGluR1 extracellular Antibody
    Anti mGluR1 extracellular Antibody AGC 006 is a highly specific antibody directed against the extracellular N terminus domain of rat mGluR1 The antibody can be used in western blot and immunocytochemistry applications and recognizes mGluR1 in rat mouse and human samples
    https://www.bioz.com/result/anti mglur1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mglur1 - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Disassembly of Shank and Homer Synaptic Clusters Is Driven by Soluble ?-Amyloid1-40 through Divergent NMDAR-Dependent Signalling Pathways"

    Article Title: Disassembly of Shank and Homer Synaptic Clusters Is Driven by Soluble ?-Amyloid1-40 through Divergent NMDAR-Dependent Signalling Pathways

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0006011

    Dispersal of synaptic mGluR1 clusters by Aβ requires NMDAR and VDCC activity. Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p
    Figure Legend Snippet: Dispersal of synaptic mGluR1 clusters by Aβ requires NMDAR and VDCC activity. Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p

    Techniques Used: Activity Assay

    2) Product Images from "Metabotropic glutamate receptors (mGluR5) activate TRPC channels to improve the regularity of the respiratory rhythm generated by the pre-B?tzinger Complex in mice"

    Article Title: Metabotropic glutamate receptors (mGluR5) activate TRPC channels to improve the regularity of the respiratory rhythm generated by the pre-B?tzinger Complex in mice

    Journal: The European Journal of Neuroscience

    doi: 10.1111/j.1460-9568.2012.08091.x

    Blocking mGluR5 with MPEP increases inspiratory neuron burst irregularity and decreases neuron burst frequency while additionally blocking mGluR1 with LY367385 does not further alter these parameters
    Figure Legend Snippet: Blocking mGluR5 with MPEP increases inspiratory neuron burst irregularity and decreases neuron burst frequency while additionally blocking mGluR1 with LY367385 does not further alter these parameters

    Techniques Used: Blocking Assay

    3) Product Images from "Metabotropic glutamate receptor-1 regulates inflammation in triple negative breast cancer"

    Article Title: Metabotropic glutamate receptor-1 regulates inflammation in triple negative breast cancer

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-34502-8

    GRM1 and mGluR1 expression in TNBC. ( A ) Knockdown of GRM1 was accomplished by infecting MDA-MB-231 cells with GIPZ shRNA Lentiviral vectors containing a puromycin resistance gene and shRNA against GRM1 or a non-silencing shRNA construct (NS). GRM1 overexpression was accomplished by infecting MDA-MB-468 cells with pLenti6.3/V5-TOPO vectors containing a blasticidin resistance gene and GRM1 or LACZ construct ( LACZ ). Cells were stably selected with puromycin (1 μg/ml) or blasticidin (5 μg/ml) for 7 days and levels of GRM1 message ( A ) or its corresponding protein, mGluR1 ( B ) were measured by QPCR or Western blot, respectively. mGluR1 expression in SUM159, BT549 and non-transduced MDA-MB-231 TNBC cell lines were also detected. Results in A represent n = 2 experiments and are expressed as the mean ± SEM where *is P
    Figure Legend Snippet: GRM1 and mGluR1 expression in TNBC. ( A ) Knockdown of GRM1 was accomplished by infecting MDA-MB-231 cells with GIPZ shRNA Lentiviral vectors containing a puromycin resistance gene and shRNA against GRM1 or a non-silencing shRNA construct (NS). GRM1 overexpression was accomplished by infecting MDA-MB-468 cells with pLenti6.3/V5-TOPO vectors containing a blasticidin resistance gene and GRM1 or LACZ construct ( LACZ ). Cells were stably selected with puromycin (1 μg/ml) or blasticidin (5 μg/ml) for 7 days and levels of GRM1 message ( A ) or its corresponding protein, mGluR1 ( B ) were measured by QPCR or Western blot, respectively. mGluR1 expression in SUM159, BT549 and non-transduced MDA-MB-231 TNBC cell lines were also detected. Results in A represent n = 2 experiments and are expressed as the mean ± SEM where *is P

    Techniques Used: Expressing, Multiple Displacement Amplification, shRNA, Construct, Over Expression, Stable Transfection, Real-time Polymerase Chain Reaction, Western Blot

    Related Articles

    Incubation:

    Article Title: Disassembly of Shank and Homer Synaptic Clusters Is Driven by Soluble ?-Amyloid1-40 through Divergent NMDAR-Dependent Signalling Pathways
    Article Snippet: .. Cells were incubated with anti-mGluR1 (1∶100, Alomone laboratories, Jerusalem, Israel) for 1 h at 25°C and with Alexa-488 conjugated goat anti-rabbit IgG (1∶500). ..

    Immunodetection:

    Article Title: Metabotropic glutamate receptor-1 regulates inflammation in triple negative breast cancer
    Article Snippet: .. Immunodetection of mGluR1 was performed using anti-mGluR1 antibody (Alamone Labs, Jerusalum, Israel) with appropriate secondary antibody and detected by chemiluminescence. ..

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  • News
  • Press Release
  • Team
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  • Contact
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  • 94
    Alomone Labs anti mglur1
    Dispersal of synaptic <t>mGluR1</t> clusters by Aβ requires NMDAR and VDCC activity. Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p
    Anti Mglur1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mglur1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mglur1 - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Dispersal of synaptic mGluR1 clusters by Aβ requires NMDAR and VDCC activity. Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p

    Journal: PLoS ONE

    Article Title: Disassembly of Shank and Homer Synaptic Clusters Is Driven by Soluble ?-Amyloid1-40 through Divergent NMDAR-Dependent Signalling Pathways

    doi: 10.1371/journal.pone.0006011

    Figure Lengend Snippet: Dispersal of synaptic mGluR1 clusters by Aβ requires NMDAR and VDCC activity. Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p

    Article Snippet: Cells were incubated with anti-mGluR1 (1∶100, Alomone laboratories, Jerusalem, Israel) for 1 h at 25°C and with Alexa-488 conjugated goat anti-rabbit IgG (1∶500).

    Techniques: Activity Assay

    Blocking mGluR5 with MPEP increases inspiratory neuron burst irregularity and decreases neuron burst frequency while additionally blocking mGluR1 with LY367385 does not further alter these parameters

    Journal: The European Journal of Neuroscience

    Article Title: Metabotropic glutamate receptors (mGluR5) activate TRPC channels to improve the regularity of the respiratory rhythm generated by the pre-B?tzinger Complex in mice

    doi: 10.1111/j.1460-9568.2012.08091.x

    Figure Lengend Snippet: Blocking mGluR5 with MPEP increases inspiratory neuron burst irregularity and decreases neuron burst frequency while additionally blocking mGluR1 with LY367385 does not further alter these parameters

    Article Snippet: The membranes were blocked overnight at +4°C with 2% non-fat dried milk (NFDM) (Bio-Rad Labs, Hercules, CA, USA) and 2% BSA (Sigma Aldrich, Milwaukee, WI, USA) in Tris buffered saline, pH = 7.5, containing 0.1% Tween-20 (TBS-T) buffer and immuno-blotted for 2h at room temperature with either anti-mGluR5 antibody (1:400) (Abcam, Cambridge, MA, USA), anti-mGluR1 antibody (1:800) (Alomone labs, Jerusalem, Israel), or anti-GAPDH antibody (1:1000, Abcam, USA) in 2% non-fat dried milk in TBS-T buffer.

    Techniques: Blocking Assay

    GRM1 and mGluR1 expression in TNBC. ( A ) Knockdown of GRM1 was accomplished by infecting MDA-MB-231 cells with GIPZ shRNA Lentiviral vectors containing a puromycin resistance gene and shRNA against GRM1 or a non-silencing shRNA construct (NS). GRM1 overexpression was accomplished by infecting MDA-MB-468 cells with pLenti6.3/V5-TOPO vectors containing a blasticidin resistance gene and GRM1 or LACZ construct ( LACZ ). Cells were stably selected with puromycin (1 μg/ml) or blasticidin (5 μg/ml) for 7 days and levels of GRM1 message ( A ) or its corresponding protein, mGluR1 ( B ) were measured by QPCR or Western blot, respectively. mGluR1 expression in SUM159, BT549 and non-transduced MDA-MB-231 TNBC cell lines were also detected. Results in A represent n = 2 experiments and are expressed as the mean ± SEM where *is P

    Journal: Scientific Reports

    Article Title: Metabotropic glutamate receptor-1 regulates inflammation in triple negative breast cancer

    doi: 10.1038/s41598-018-34502-8

    Figure Lengend Snippet: GRM1 and mGluR1 expression in TNBC. ( A ) Knockdown of GRM1 was accomplished by infecting MDA-MB-231 cells with GIPZ shRNA Lentiviral vectors containing a puromycin resistance gene and shRNA against GRM1 or a non-silencing shRNA construct (NS). GRM1 overexpression was accomplished by infecting MDA-MB-468 cells with pLenti6.3/V5-TOPO vectors containing a blasticidin resistance gene and GRM1 or LACZ construct ( LACZ ). Cells were stably selected with puromycin (1 μg/ml) or blasticidin (5 μg/ml) for 7 days and levels of GRM1 message ( A ) or its corresponding protein, mGluR1 ( B ) were measured by QPCR or Western blot, respectively. mGluR1 expression in SUM159, BT549 and non-transduced MDA-MB-231 TNBC cell lines were also detected. Results in A represent n = 2 experiments and are expressed as the mean ± SEM where *is P

    Article Snippet: Immunodetection of mGluR1 was performed using anti-mGluR1 antibody (Alamone Labs, Jerusalum, Israel) with appropriate secondary antibody and detected by chemiluminescence.

    Techniques: Expressing, Multiple Displacement Amplification, shRNA, Construct, Over Expression, Stable Transfection, Real-time Polymerase Chain Reaction, Western Blot