anti mglur1 antibody (Alomone Labs)

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Alomone Labs anti mglur1 antibody
Blocking mGluR5 with MPEP increases inspiratory neuron burst irregularity and decreases neuron burst frequency while additionally blocking <t>mGluR1</t> with LY367385 does not further alter these parameters

Blocking mGluR5 with MPEP increases inspiratory neuron burst irregularity and decreases neuron burst frequency while additionally blocking <t>mGluR1</t> with LY367385 does not further alter these parameters

Anti Mglur1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mglur1 antibody/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99

anti mglur1 antibody - by Bioz Stars, 2022-06

93/100 stars

Images

1) Product Images from "Metabotropic glutamate receptors (mGluR5) activate TRPC channels to improve the regularity of the respiratory rhythm generated by the pre-B?tzinger Complex in mice"

Article Title: Metabotropic glutamate receptors (mGluR5) activate TRPC channels to improve the regularity of the respiratory rhythm generated by the pre-B?tzinger Complex in mice

Journal: The European Journal of Neuroscience

doi: 10.1111/j.1460-9568.2012.08091.x

Figure Legend Snippet: Blocking mGluR5 with MPEP increases inspiratory neuron burst irregularity and decreases neuron burst frequency while additionally blocking mGluR1 with LY367385 does not further alter these parameters

Techniques Used: Blocking Assay

2) Product Images from "Metabotropic glutamate receptor-1 regulates inflammation in triple negative breast cancer"

Article Title: Metabotropic glutamate receptor-1 regulates inflammation in triple negative breast cancer

Journal: Scientific Reports

doi: 10.1038/s41598-018-34502-8

GRM1 and mGluR1 expression in TNBC. ( A ) Knockdown of GRM1 was accomplished by infecting MDA-MB-231 cells with GIPZ shRNA Lentiviral vectors containing a puromycin resistance gene and shRNA against GRM1 or a non-silencing shRNA construct (NS). GRM1 overexpression was accomplished by infecting MDA-MB-468 cells with pLenti6.3/V5-TOPO vectors containing a blasticidin resistance gene and GRM1 or LACZ construct ( LACZ ). Cells were stably selected with puromycin (1 μg/ml) or blasticidin (5 μg/ml) for 7 days and levels of GRM1 message ( A ) or its corresponding protein, mGluR1 ( B ) were measured by QPCR or Western blot, respectively. mGluR1 expression in SUM159, BT549 and non-transduced MDA-MB-231 TNBC cell lines were also detected. Results in A represent n = 2 experiments and are expressed as the mean ± SEM where *is P

Figure Legend Snippet: GRM1 and mGluR1 expression in TNBC. ( A ) Knockdown of GRM1 was accomplished by infecting MDA-MB-231 cells with GIPZ shRNA Lentiviral vectors containing a puromycin resistance gene and shRNA against GRM1 or a non-silencing shRNA construct (NS). GRM1 overexpression was accomplished by infecting MDA-MB-468 cells with pLenti6.3/V5-TOPO vectors containing a blasticidin resistance gene and GRM1 or LACZ construct ( LACZ ). Cells were stably selected with puromycin (1 μg/ml) or blasticidin (5 μg/ml) for 7 days and levels of GRM1 message ( A ) or its corresponding protein, mGluR1 ( B ) were measured by QPCR or Western blot, respectively. mGluR1 expression in SUM159, BT549 and non-transduced MDA-MB-231 TNBC cell lines were also detected. Results in A represent n = 2 experiments and are expressed as the mean ± SEM where *is P

Techniques Used: Expressing, Multiple Displacement Amplification, shRNA, Construct, Over Expression, Stable Transfection, Real-time Polymerase Chain Reaction, Western Blot

3) Product Images from "Stabilization of spine Synaptopodin by mGluR1 is required for mGluR-LTD"

Article Title: Stabilization of spine Synaptopodin by mGluR1 is required for mGluR-LTD

Journal: bioRxiv

doi: 10.1101/2021.09.14.460352

mGluR-LTD induces loss of mushroom spines through activation of mGluR1. A. Representative images of hippocampal neurons treated with vehicle or DHPG in the presence of Bay (30 min recovery) and labeled with DiIC 18 ; arrowheads indicate mushroom spines, scale bars, 10 µm. B. Quantification of mushroom spine density from images like those in (a): mean ± SD, vehicle 1.7 ± 0.50 (n=55), DHPG+Bay 1.5 ± 0.57 (n=49), N=25 cells per group, p =0.1044, two-sided permutation t test. C. Representative images of hippocampal neurons treated with vehicle or DHPG in the presence of MPEP (30 min recovery) and labeled with DiIC 18 ; scale bars, 10 µm. D. Quantification of mushroom spine density from images like those in (c): mean ± SD, vehicle 1.7 ± 0.57 (n=53), DHPG+MPEP 1.3 ± 0.49 (n=40) from 3 experiments, p =0.0032, two-sided permutation t test.

Figure Legend Snippet: mGluR-LTD induces loss of mushroom spines through activation of mGluR1. A. Representative images of hippocampal neurons treated with vehicle or DHPG in the presence of Bay (30 min recovery) and labeled with DiIC 18 ; arrowheads indicate mushroom spines, scale bars, 10 µm. B. Quantification of mushroom spine density from images like those in (a): mean ± SD, vehicle 1.7 ± 0.50 (n=55), DHPG+Bay 1.5 ± 0.57 (n=49), N=25 cells per group, p =0.1044, two-sided permutation t test. C. Representative images of hippocampal neurons treated with vehicle or DHPG in the presence of MPEP (30 min recovery) and labeled with DiIC 18 ; scale bars, 10 µm. D. Quantification of mushroom spine density from images like those in (c): mean ± SD, vehicle 1.7 ± 0.57 (n=53), DHPG+MPEP 1.3 ± 0.49 (n=40) from 3 experiments, p =0.0032, two-sided permutation t test.

Techniques Used: Activation Assay, Labeling

Reduced density and growth of mushroom spines in mice lacking mGluR1. A. Representative images of DiIC 18 -labeled dendritic branches in the brain cortex of adult Grm1 WT and Grm1 KO mice; scale bars 10 µm. B. Quantification of spine density per 10 µm in the cortex of Grm1 WT and Grm1 KO mice from images as in (a). Juvenile (∼1.5 month-old), mean ± SD thin spines WT 6.8 ± 1.7 (n=32 dendritic branches), KO 7.8 ± 1.9 (n=25), (*) p =0.025; mushroom spines WT 1.9 ± 0.98 (n=34), KO 1.10 ± 0.39 (n=19), N=2 mice per group; (***) p

Figure Legend Snippet: Reduced density and growth of mushroom spines in mice lacking mGluR1. A. Representative images of DiIC 18 -labeled dendritic branches in the brain cortex of adult Grm1 WT and Grm1 KO mice; scale bars 10 µm. B. Quantification of spine density per 10 µm in the cortex of Grm1 WT and Grm1 KO mice from images as in (a). Juvenile (∼1.5 month-old), mean ± SD thin spines WT 6.8 ± 1.7 (n=32 dendritic branches), KO 7.8 ± 1.9 (n=25), (*) p =0.025; mushroom spines WT 1.9 ± 0.98 (n=34), KO 1.10 ± 0.39 (n=19), N=2 mice per group; (***) p

Techniques Used: Mouse Assay, Labeling

mGluR1 enables Synpo stabilization at excitatory spine synapses. A. Synpo co-precipitates with mGluR1: representative immunoblot probed for Synpo of immunoprecipitation (Ip) with anti-mGluR1 or control IgG from lysates of brain cortex of adult WT mice. B. Representative images of hippocampal neurons labeled for mGluR1, Synpo and MAP2: mGluR1 puncta are found in apposition to Synpo. Scale bar 50 µm; inset, 10 µm. C. Co-precipitation of PSD95 with Synpo in Grm1 WT and Grm1 KO mice: representative blot probed for PSD95 of immunoprecipitation with anti-Synpo antibody from lysates of brain cortex of adult Grm1 WT and Grm1 KO mice. Input, 5% of Ip lysate; Tubulin (Tub), loading control. D. Quantification of PSD95 co-precipitation with Synpo from blots as in (b). Mean ± SE, KO/WT PSD95 0.54 ± 0.12, KO/WT Synpo 1.2 ± 0.16, N=3 mice per group, two-tailed unpaired t-test.

Figure Legend Snippet: mGluR1 enables Synpo stabilization at excitatory spine synapses. A. Synpo co-precipitates with mGluR1: representative immunoblot probed for Synpo of immunoprecipitation (Ip) with anti-mGluR1 or control IgG from lysates of brain cortex of adult WT mice. B. Representative images of hippocampal neurons labeled for mGluR1, Synpo and MAP2: mGluR1 puncta are found in apposition to Synpo. Scale bar 50 µm; inset, 10 µm. C. Co-precipitation of PSD95 with Synpo in Grm1 WT and Grm1 KO mice: representative blot probed for PSD95 of immunoprecipitation with anti-Synpo antibody from lysates of brain cortex of adult Grm1 WT and Grm1 KO mice. Input, 5% of Ip lysate; Tubulin (Tub), loading control. D. Quantification of PSD95 co-precipitation with Synpo from blots as in (b). Mean ± SE, KO/WT PSD95 0.54 ± 0.12, KO/WT Synpo 1.2 ± 0.16, N=3 mice per group, two-tailed unpaired t-test.

Techniques Used: Immunoprecipitation, Mouse Assay, Labeling, Two Tailed Test

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anti mglur1 antibody (Alomone Labs)

Alomone Labs anti mglur1 antibody

anti mglur1 antibody - by Bioz Stars, 2022-06

93/100 stars

Buy from Supplier

Image Search Results

Journal: The European Journal of Neuroscience

Article Title: Metabotropic glutamate receptors (mGluR5) activate TRPC channels to improve the regularity of the respiratory rhythm generated by the pre-B?tzinger Complex in mice

doi: 10.1111/j.1460-9568.2012.08091.x

Figure Lengend Snippet: Blocking mGluR5 with MPEP increases inspiratory neuron burst irregularity and decreases neuron burst frequency while additionally blocking mGluR1 with LY367385 does not further alter these parameters

Article Snippet: The membranes were blocked overnight at +4°C with 2% non-fat dried milk (NFDM) (Bio-Rad Labs, Hercules, CA, USA) and 2% BSA (Sigma Aldrich, Milwaukee, WI, USA) in Tris buffered saline, pH = 7.5, containing 0.1% Tween-20 (TBS-T) buffer and immuno-blotted for 2h at room temperature with either anti-mGluR5 antibody (1:400) (Abcam, Cambridge, MA, USA), anti-mGluR1 antibody (1:800) (Alomone labs, Jerusalem, Israel), or anti-GAPDH antibody (1:1000, Abcam, USA) in 2% non-fat dried milk in TBS-T buffer.

Techniques: Blocking Assay

Journal: Scientific Reports

Article Title: Metabotropic glutamate receptor-1 regulates inflammation in triple negative breast cancer

doi: 10.1038/s41598-018-34502-8

Figure Lengend Snippet: GRM1 and mGluR1 expression in TNBC. ( A ) Knockdown of GRM1 was accomplished by infecting MDA-MB-231 cells with GIPZ shRNA Lentiviral vectors containing a puromycin resistance gene and shRNA against GRM1 or a non-silencing shRNA construct (NS). GRM1 overexpression was accomplished by infecting MDA-MB-468 cells with pLenti6.3/V5-TOPO vectors containing a blasticidin resistance gene and GRM1 or LACZ construct ( LACZ ). Cells were stably selected with puromycin (1 μg/ml) or blasticidin (5 μg/ml) for 7 days and levels of GRM1 message ( A ) or its corresponding protein, mGluR1 ( B ) were measured by QPCR or Western blot, respectively. mGluR1 expression in SUM159, BT549 and non-transduced MDA-MB-231 TNBC cell lines were also detected. Results in A represent n = 2 experiments and are expressed as the mean ± SEM where *is P

Article Snippet: Immunodetection of mGluR1 was performed using anti-mGluR1 antibody (Alamone Labs, Jerusalum, Israel) with appropriate secondary antibody and detected by chemiluminescence.

Techniques: Expressing, Multiple Displacement Amplification, shRNA, Construct, Over Expression, Stable Transfection, Real-time Polymerase Chain Reaction, Western Blot

Journal: bioRxiv

Article Title: Stabilization of spine Synaptopodin by mGluR1 is required for mGluR-LTD

doi: 10.1101/2021.09.14.460352

Figure Lengend Snippet: mGluR-LTD induces loss of mushroom spines through activation of mGluR1. A. Representative images of hippocampal neurons treated with vehicle or DHPG in the presence of Bay (30 min recovery) and labeled with DiIC 18 ; arrowheads indicate mushroom spines, scale bars, 10 µm. B. Quantification of mushroom spine density from images like those in (a): mean ± SD, vehicle 1.7 ± 0.50 (n=55), DHPG+Bay 1.5 ± 0.57 (n=49), N=25 cells per group, p =0.1044, two-sided permutation t test. C. Representative images of hippocampal neurons treated with vehicle or DHPG in the presence of MPEP (30 min recovery) and labeled with DiIC 18 ; scale bars, 10 µm. D. Quantification of mushroom spine density from images like those in (c): mean ± SD, vehicle 1.7 ± 0.57 (n=53), DHPG+MPEP 1.3 ± 0.49 (n=40) from 3 experiments, p =0.0032, two-sided permutation t test.

Article Snippet: The following primary antibodies were used: rabbit anti-Synaptopodin (1/1000), goat anti-Synaptopodin (1/200; Santa Cruz Biotech, RRID: AB_2201166), rabbit mGluR1 (1/500, Alomone labs, RRID:AB_2039984; 1/10,000 RRID:AB_2571736), mouse anti-PSD95 (1/400; Antibodies Inc., RRID: AB_2292909), mouse anti-γ-Tubulin (1/2,500; Sigma Aldrich, RRID: AB_477584).

Techniques: Activation Assay, Labeling

Journal: bioRxiv

Article Title: Stabilization of spine Synaptopodin by mGluR1 is required for mGluR-LTD

doi: 10.1101/2021.09.14.460352

Figure Lengend Snippet: Reduced density and growth of mushroom spines in mice lacking mGluR1. A. Representative images of DiIC 18 -labeled dendritic branches in the brain cortex of adult Grm1 WT and Grm1 KO mice; scale bars 10 µm. B. Quantification of spine density per 10 µm in the cortex of Grm1 WT and Grm1 KO mice from images as in (a). Juvenile (∼1.5 month-old), mean ± SD thin spines WT 6.8 ± 1.7 (n=32 dendritic branches), KO 7.8 ± 1.9 (n=25), (*) p =0.025; mushroom spines WT 1.9 ± 0.98 (n=34), KO 1.10 ± 0.39 (n=19), N=2 mice per group; (***) p

Techniques: Mouse Assay, Labeling

Journal: bioRxiv

Article Title: Stabilization of spine Synaptopodin by mGluR1 is required for mGluR-LTD

doi: 10.1101/2021.09.14.460352

Figure Lengend Snippet: mGluR1 enables Synpo stabilization at excitatory spine synapses. A. Synpo co-precipitates with mGluR1: representative immunoblot probed for Synpo of immunoprecipitation (Ip) with anti-mGluR1 or control IgG from lysates of brain cortex of adult WT mice. B. Representative images of hippocampal neurons labeled for mGluR1, Synpo and MAP2: mGluR1 puncta are found in apposition to Synpo. Scale bar 50 µm; inset, 10 µm. C. Co-precipitation of PSD95 with Synpo in Grm1 WT and Grm1 KO mice: representative blot probed for PSD95 of immunoprecipitation with anti-Synpo antibody from lysates of brain cortex of adult Grm1 WT and Grm1 KO mice. Input, 5% of Ip lysate; Tubulin (Tub), loading control. D. Quantification of PSD95 co-precipitation with Synpo from blots as in (b). Mean ± SE, KO/WT PSD95 0.54 ± 0.12, KO/WT Synpo 1.2 ± 0.16, N=3 mice per group, two-tailed unpaired t-test.

Techniques: Immunoprecipitation, Mouse Assay, Labeling, Two Tailed Test