anti mglur1  (Alomone Labs)


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    Alomone Labs anti mglur1
    Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface <t>mGluR1</t> before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p<0.05); pre-treatment with either MK801 (10 µM) or verapamil (50 µM) blocked this effect (102.9±10.5%, for MK801+Aβ vs. MK alone; 109.8±4.1%, for verapamil+Aβ vs. verapamil alone). (C, D) Aβ treatment results in decreased synaptic localization of total mGluR1 (ratio of mGluR1/synaptophysin immunopositive puncta, 47.3±5.7% of baseline, n = 600, p<0.05); pre-treatment with either MK801 or verapamil prevented this effect (102.0±7.2%, for MK801+Aβ vs. MK alone; 106.7±10.8% for verapamil+Aβ vs. verapamil alone). (C, E) Aβ treatment results in reduced size of total synaptic mGluR1 clusters (68.1±8.0%, N = 10, n = 600, p<0.05); pre-treatment with either MK801 or verapamil abolished Aβ-induced cluster shrinkage (93±11.1%, for MK801+Aβ vs. MK alone; 112.4±10.7%, for verapamil+Aβ vs. verapamil alone). Scale bar represents 5 µm.
    Anti Mglur1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mglur1 - by Bioz Stars, 2024-07
    93/100 stars

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    1) Product Images from "Disassembly of Shank and Homer Synaptic Clusters Is Driven by Soluble β-Amyloid 1-40 through Divergent NMDAR-Dependent Signalling Pathways"

    Article Title: Disassembly of Shank and Homer Synaptic Clusters Is Driven by Soluble β-Amyloid 1-40 through Divergent NMDAR-Dependent Signalling Pathways

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0006011

    Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p<0.05); pre-treatment with either MK801 (10 µM) or verapamil (50 µM) blocked this effect (102.9±10.5%, for MK801+Aβ vs. MK alone; 109.8±4.1%, for verapamil+Aβ vs. verapamil alone). (C, D) Aβ treatment results in decreased synaptic localization of total mGluR1 (ratio of mGluR1/synaptophysin immunopositive puncta, 47.3±5.7% of baseline, n = 600, p<0.05); pre-treatment with either MK801 or verapamil prevented this effect (102.0±7.2%, for MK801+Aβ vs. MK alone; 106.7±10.8% for verapamil+Aβ vs. verapamil alone). (C, E) Aβ treatment results in reduced size of total synaptic mGluR1 clusters (68.1±8.0%, N = 10, n = 600, p<0.05); pre-treatment with either MK801 or verapamil abolished Aβ-induced cluster shrinkage (93±11.1%, for MK801+Aβ vs. MK alone; 112.4±10.7%, for verapamil+Aβ vs. verapamil alone). Scale bar represents 5 µm.
    Figure Legend Snippet: Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p<0.05); pre-treatment with either MK801 (10 µM) or verapamil (50 µM) blocked this effect (102.9±10.5%, for MK801+Aβ vs. MK alone; 109.8±4.1%, for verapamil+Aβ vs. verapamil alone). (C, D) Aβ treatment results in decreased synaptic localization of total mGluR1 (ratio of mGluR1/synaptophysin immunopositive puncta, 47.3±5.7% of baseline, n = 600, p<0.05); pre-treatment with either MK801 or verapamil prevented this effect (102.0±7.2%, for MK801+Aβ vs. MK alone; 106.7±10.8% for verapamil+Aβ vs. verapamil alone). (C, E) Aβ treatment results in reduced size of total synaptic mGluR1 clusters (68.1±8.0%, N = 10, n = 600, p<0.05); pre-treatment with either MK801 or verapamil abolished Aβ-induced cluster shrinkage (93±11.1%, for MK801+Aβ vs. MK alone; 112.4±10.7%, for verapamil+Aβ vs. verapamil alone). Scale bar represents 5 µm.

    Techniques Used:


    Structured Review

    AGC Techno Glass Co Ltd 6 well plate
    6 Well Plate, supplied by AGC Techno Glass Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    AGC Techno Glass Co Ltd 6 well plates
    6 Well Plates, supplied by AGC Techno Glass Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    6 well plates - by Bioz Stars, 2024-07
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    anti mglur1  (Alomone Labs)


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    Alomone Labs anti mglur1
    Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface <t>mGluR1</t> before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p<0.05); pre-treatment with either MK801 (10 µM) or verapamil (50 µM) blocked this effect (102.9±10.5%, for MK801+Aβ vs. MK alone; 109.8±4.1%, for verapamil+Aβ vs. verapamil alone). (C, D) Aβ treatment results in decreased synaptic localization of total mGluR1 (ratio of mGluR1/synaptophysin immunopositive puncta, 47.3±5.7% of baseline, n = 600, p<0.05); pre-treatment with either MK801 or verapamil prevented this effect (102.0±7.2%, for MK801+Aβ vs. MK alone; 106.7±10.8% for verapamil+Aβ vs. verapamil alone). (C, E) Aβ treatment results in reduced size of total synaptic mGluR1 clusters (68.1±8.0%, N = 10, n = 600, p<0.05); pre-treatment with either MK801 or verapamil abolished Aβ-induced cluster shrinkage (93±11.1%, for MK801+Aβ vs. MK alone; 112.4±10.7%, for verapamil+Aβ vs. verapamil alone). Scale bar represents 5 µm.
    Anti Mglur1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mglur1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
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    anti mglur1 - by Bioz Stars, 2024-07
    93/100 stars

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    1) Product Images from "Disassembly of Shank and Homer Synaptic Clusters Is Driven by Soluble β-Amyloid 1-40 through Divergent NMDAR-Dependent Signalling Pathways"

    Article Title: Disassembly of Shank and Homer Synaptic Clusters Is Driven by Soluble β-Amyloid 1-40 through Divergent NMDAR-Dependent Signalling Pathways

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0006011

    Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p<0.05); pre-treatment with either MK801 (10 µM) or verapamil (50 µM) blocked this effect (102.9±10.5%, for MK801+Aβ vs. MK alone; 109.8±4.1%, for verapamil+Aβ vs. verapamil alone). (C, D) Aβ treatment results in decreased synaptic localization of total mGluR1 (ratio of mGluR1/synaptophysin immunopositive puncta, 47.3±5.7% of baseline, n = 600, p<0.05); pre-treatment with either MK801 or verapamil prevented this effect (102.0±7.2%, for MK801+Aβ vs. MK alone; 106.7±10.8% for verapamil+Aβ vs. verapamil alone). (C, E) Aβ treatment results in reduced size of total synaptic mGluR1 clusters (68.1±8.0%, N = 10, n = 600, p<0.05); pre-treatment with either MK801 or verapamil abolished Aβ-induced cluster shrinkage (93±11.1%, for MK801+Aβ vs. MK alone; 112.4±10.7%, for verapamil+Aβ vs. verapamil alone). Scale bar represents 5 µm.
    Figure Legend Snippet: Rat cortical neurons were treated with Aβ (1 µM) for 1 h (with or without co-treatment with the NMDAR antagonist MK801, or the VDCC blocker verapamil) and then immunostained for surface mGluR1 before fixation. (A, B) Aβ downregulates surface mGluR1 (surface cluster size 66.0±2.8%, N = 10, n = 300, p<0.05); pre-treatment with either MK801 (10 µM) or verapamil (50 µM) blocked this effect (102.9±10.5%, for MK801+Aβ vs. MK alone; 109.8±4.1%, for verapamil+Aβ vs. verapamil alone). (C, D) Aβ treatment results in decreased synaptic localization of total mGluR1 (ratio of mGluR1/synaptophysin immunopositive puncta, 47.3±5.7% of baseline, n = 600, p<0.05); pre-treatment with either MK801 or verapamil prevented this effect (102.0±7.2%, for MK801+Aβ vs. MK alone; 106.7±10.8% for verapamil+Aβ vs. verapamil alone). (C, E) Aβ treatment results in reduced size of total synaptic mGluR1 clusters (68.1±8.0%, N = 10, n = 600, p<0.05); pre-treatment with either MK801 or verapamil abolished Aβ-induced cluster shrinkage (93±11.1%, for MK801+Aβ vs. MK alone; 112.4±10.7%, for verapamil+Aβ vs. verapamil alone). Scale bar represents 5 µm.

    Techniques Used:

    metabotropic glutamate receptors  (Alomone Labs)


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    Alomone Labs metabotropic glutamate receptors
    Responses are plotted as percent change from the baseline fEPSPs as a function of time. Numbers on the representative traces show the time on the graph at which they were recorded. A) SKF81297-induced LTP in the cocaine CPP group (clear triangles, 151.4±8.8%, * p <0.05, n = 6) is completely blocked by the PLD-linked <t>mGluR</t> antagonist (PCCG-13, filled triangles, 95.0±9.2%, n = 6). B) <t>mGluR1</t> receptor antagonist (LY367385, filled triangles, 106.0±6.7%, n = 6) blocks the SKF81297-induced LTP (clear triangles, * p <0.05, n = 6). C) The mGluR5 antagonist (MPEP, filled triangles, 122.7±5.6%, n = 6) significantly reduces but does not abolish the SKF81297-induced LTP (clear triangles, * p <0.05, n = 6). D) PLC antagonist (U-73122, filled triangles, 128.2±6.1%, n = 6), reduces but does not eliminate the SKF81297-induced LTP (clear triangles, * p <0.05, n = 6). For comparison, panels A and B use same data graphs and fEPSP traces for the slices from cocaine CPP group superfused with SKF81297 as shown in , and .
    Metabotropic Glutamate Receptors, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Dopamine-Induced Plasticity, Phospholipase D (PLD) Activity and Cocaine-Cue Behavior Depend on PLD-Linked Metabotropic Glutamate Receptors in Amygdala"

    Article Title: Dopamine-Induced Plasticity, Phospholipase D (PLD) Activity and Cocaine-Cue Behavior Depend on PLD-Linked Metabotropic Glutamate Receptors in Amygdala

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0025639

    Responses are plotted as percent change from the baseline fEPSPs as a function of time. Numbers on the representative traces show the time on the graph at which they were recorded. A) SKF81297-induced LTP in the cocaine CPP group (clear triangles, 151.4±8.8%, * p <0.05, n = 6) is completely blocked by the PLD-linked mGluR antagonist (PCCG-13, filled triangles, 95.0±9.2%, n = 6). B) mGluR1 receptor antagonist (LY367385, filled triangles, 106.0±6.7%, n = 6) blocks the SKF81297-induced LTP (clear triangles, * p <0.05, n = 6). C) The mGluR5 antagonist (MPEP, filled triangles, 122.7±5.6%, n = 6) significantly reduces but does not abolish the SKF81297-induced LTP (clear triangles, * p <0.05, n = 6). D) PLC antagonist (U-73122, filled triangles, 128.2±6.1%, n = 6), reduces but does not eliminate the SKF81297-induced LTP (clear triangles, * p <0.05, n = 6). For comparison, panels A and B use same data graphs and fEPSP traces for the slices from cocaine CPP group superfused with SKF81297 as shown in , and .
    Figure Legend Snippet: Responses are plotted as percent change from the baseline fEPSPs as a function of time. Numbers on the representative traces show the time on the graph at which they were recorded. A) SKF81297-induced LTP in the cocaine CPP group (clear triangles, 151.4±8.8%, * p <0.05, n = 6) is completely blocked by the PLD-linked mGluR antagonist (PCCG-13, filled triangles, 95.0±9.2%, n = 6). B) mGluR1 receptor antagonist (LY367385, filled triangles, 106.0±6.7%, n = 6) blocks the SKF81297-induced LTP (clear triangles, * p <0.05, n = 6). C) The mGluR5 antagonist (MPEP, filled triangles, 122.7±5.6%, n = 6) significantly reduces but does not abolish the SKF81297-induced LTP (clear triangles, * p <0.05, n = 6). D) PLC antagonist (U-73122, filled triangles, 128.2±6.1%, n = 6), reduces but does not eliminate the SKF81297-induced LTP (clear triangles, * p <0.05, n = 6). For comparison, panels A and B use same data graphs and fEPSP traces for the slices from cocaine CPP group superfused with SKF81297 as shown in , and .

    Techniques Used:

    The dotted line indicates PLD activity associated with control slices (no EtOH added) which was determined for each animal and used to calculate the change in PLD activity levels with EtOH and/or drug application. Basal levels represent the increase in PLD activity observed in the EtOH-treated slices compared to the no EtOH controls; * p <0.05 compared to the corresponding saline control and # p <0.05 compared to the cocaine CPP group basal PLD activity. Basal PLD activity was significantly increased (*** p <0.001, n = 50) in the cocaine CPP group (dark bars, 527.3±94.3) compared to the saline-treated group (white bars, 142.6±36.9). SKF81297, the D1/5R agonist, application increased the basal levels in the cocaine CPP group significantly (1722.0±176.9, n = 12, # p <0.05) compared to the basal PLD activity observed with EtOH treatment alone in the same experimental group. The D1/5R antagonist, SCH23390, completely blocked basal PLD activity (91.2±21.9, n = 12, ## p <0.01) in the cocaine CPP group. A similar reduction in PEtOH levels was observed with application of either the PLD-linked mGluR antagonist, PCCG-13 (62.9±10.6, n = 7, ## p <0.01) or the mGluR1 antagonist, LY367385 (75.0±13.9, n = 12, ## p <0.01), while the mGluR5 antagonist, MPEP, did not decrease basal PLD activity (305.7±31.5, n = 7, ns) within the cocaine CPP group but were significantly increased compared to (* p <0.05) the saline treated group. Applications of SKF81297 (184.9±30.5, n = 12), SCH23390 (84.9±38.9, n = 12), MPEP (74.2±16.3, n = 7), LY367385 (94.7±18.9, n = 12) and PCCG-13 (132.5±18.4, n = 7) did not significantly alter the PEtOH levels in the saline-treated group compared to the basal activity levels. Inset is a depiction of the triangular excision performed to isolate amygdala (bilaterally for each animal, each slice) containing the basolateral (BLA), the central (CeA) and the lateral (LA) subregions from three serial coronal slices (350 µm) beginning −2.30 mm to −2.80 mm from Bregma .
    Figure Legend Snippet: The dotted line indicates PLD activity associated with control slices (no EtOH added) which was determined for each animal and used to calculate the change in PLD activity levels with EtOH and/or drug application. Basal levels represent the increase in PLD activity observed in the EtOH-treated slices compared to the no EtOH controls; * p <0.05 compared to the corresponding saline control and # p <0.05 compared to the cocaine CPP group basal PLD activity. Basal PLD activity was significantly increased (*** p <0.001, n = 50) in the cocaine CPP group (dark bars, 527.3±94.3) compared to the saline-treated group (white bars, 142.6±36.9). SKF81297, the D1/5R agonist, application increased the basal levels in the cocaine CPP group significantly (1722.0±176.9, n = 12, # p <0.05) compared to the basal PLD activity observed with EtOH treatment alone in the same experimental group. The D1/5R antagonist, SCH23390, completely blocked basal PLD activity (91.2±21.9, n = 12, ## p <0.01) in the cocaine CPP group. A similar reduction in PEtOH levels was observed with application of either the PLD-linked mGluR antagonist, PCCG-13 (62.9±10.6, n = 7, ## p <0.01) or the mGluR1 antagonist, LY367385 (75.0±13.9, n = 12, ## p <0.01), while the mGluR5 antagonist, MPEP, did not decrease basal PLD activity (305.7±31.5, n = 7, ns) within the cocaine CPP group but were significantly increased compared to (* p <0.05) the saline treated group. Applications of SKF81297 (184.9±30.5, n = 12), SCH23390 (84.9±38.9, n = 12), MPEP (74.2±16.3, n = 7), LY367385 (94.7±18.9, n = 12) and PCCG-13 (132.5±18.4, n = 7) did not significantly alter the PEtOH levels in the saline-treated group compared to the basal activity levels. Inset is a depiction of the triangular excision performed to isolate amygdala (bilaterally for each animal, each slice) containing the basolateral (BLA), the central (CeA) and the lateral (LA) subregions from three serial coronal slices (350 µm) beginning −2.30 mm to −2.80 mm from Bregma .

    Techniques Used: Activity Assay

    sars cov 2  (Danaher Inc)


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    Danaher Inc sars cov 2
    Sars Cov 2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sars cov 2 2019 ncov cdc n1 qpcr probe mixture  (Integrated DNA Technologies)


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    Integrated DNA Technologies sars cov 2 2019 ncov cdc n1 qpcr probe mixture
    Sars Cov 2 2019 Ncov Cdc N1 Qpcr Probe Mixture, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit mglur1  (Alomone Labs)


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    Alomone Labs rabbit mglur1
    Rabbit Mglur1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Revvity Signals human srrm2
    List of the top 52 proteins sorted by SPK-CEN ratio
    Human Srrm2, supplied by Revvity Signals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Tyramide signal amplification mass spectrometry (TSA-MS) ratio identifies nuclear speckle proteins"

    Article Title: Tyramide signal amplification mass spectrometry (TSA-MS) ratio identifies nuclear speckle proteins

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.201910207

    List of the top 52 proteins sorted by SPK-CEN ratio
    Figure Legend Snippet: List of the top 52 proteins sorted by SPK-CEN ratio

    Techniques Used: Binding Assay, RNA Binding Assay

    MFAP1 KD specifically increases nuclear speckle size. (A) Western blots showing RNAi depletion of the indicated proteins (SRRM2, ZNF207, or PRPF38A) in U2OS cells. (B and C) Western blot showing the depletion of MFAP1 in Tig3 (B) or CHO (C) cells. Tubulin was detected as loading control. (D) Representative anti-SON immunofluorescence images of U2OS cells following siRNA treatment as indicated. (E) Representative anti-SON and anti-SC35 coimmunostaining of U2OS cells transfected with control siRNA (siCTRL) or siRNA against SRRM2 (siSRRM2). (F and G) Anti-SON immunofluorescence images of Tig3 cells (F) or CHO cells (G) after transfection with control siRNA (siCTRL) or siRNA against MFAP1 (siMFAP1). DNA (blue) was stained with DAPI. Scale bars: 10 µm.
    Figure Legend Snippet: MFAP1 KD specifically increases nuclear speckle size. (A) Western blots showing RNAi depletion of the indicated proteins (SRRM2, ZNF207, or PRPF38A) in U2OS cells. (B and C) Western blot showing the depletion of MFAP1 in Tig3 (B) or CHO (C) cells. Tubulin was detected as loading control. (D) Representative anti-SON immunofluorescence images of U2OS cells following siRNA treatment as indicated. (E) Representative anti-SON and anti-SC35 coimmunostaining of U2OS cells transfected with control siRNA (siCTRL) or siRNA against SRRM2 (siSRRM2). (F and G) Anti-SON immunofluorescence images of Tig3 cells (F) or CHO cells (G) after transfection with control siRNA (siCTRL) or siRNA against MFAP1 (siMFAP1). DNA (blue) was stained with DAPI. Scale bars: 10 µm.

    Techniques Used: Western Blot, Immunofluorescence, Transfection, Staining


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    AGC Inc polystyrene dish
    Polystyrene Dish, supplied by AGC Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti mglur1  (Alomone Labs)


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    Alomone Labs anti mglur1
    Anti Mglur1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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