polyclonal rabbit anti glua2  (Alomone Labs)


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    Alomone Labs polyclonal rabbit anti glua2
    Activation of EphB2/ephrinB2 reverse signaling induces <t>GluA2-containing</t> AMPA receptor trafficking. A , B , Representative traces showing the changes in amplitude of evoked EPSCs, mediated by AMPA receptors, recorded from four different RGCs in retinal slices at holding potentials of +40 and −60 mV with ( A ) or without ( B ) spermine in the pipette solution. The eyes were intravitreally injected respectively with control-Fc (Ctr-Fc) and EphB2-Fc (2 μl, 1 μg/μl), and the recordings were made 5–7 d after the injections. The current amplitudes were all normalized to that of Ctr-Fc at −60 mV. C , Bar chart showing the average ratios of current amplitudes at +40/−60 mV in RGCs obtained from Ctr-Fc- and EphB2-Fc-injected retinae with or without spermine in the pipette solution. * p
    Polyclonal Rabbit Anti Glua2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti glua2/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti glua2 - by Bioz Stars, 2022-05
    92/100 stars

    Images

    1) Product Images from "GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model"

    Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.4376-14.2015

    Activation of EphB2/ephrinB2 reverse signaling induces GluA2-containing AMPA receptor trafficking. A , B , Representative traces showing the changes in amplitude of evoked EPSCs, mediated by AMPA receptors, recorded from four different RGCs in retinal slices at holding potentials of +40 and −60 mV with ( A ) or without ( B ) spermine in the pipette solution. The eyes were intravitreally injected respectively with control-Fc (Ctr-Fc) and EphB2-Fc (2 μl, 1 μg/μl), and the recordings were made 5–7 d after the injections. The current amplitudes were all normalized to that of Ctr-Fc at −60 mV. C , Bar chart showing the average ratios of current amplitudes at +40/−60 mV in RGCs obtained from Ctr-Fc- and EphB2-Fc-injected retinae with or without spermine in the pipette solution. * p
    Figure Legend Snippet: Activation of EphB2/ephrinB2 reverse signaling induces GluA2-containing AMPA receptor trafficking. A , B , Representative traces showing the changes in amplitude of evoked EPSCs, mediated by AMPA receptors, recorded from four different RGCs in retinal slices at holding potentials of +40 and −60 mV with ( A ) or without ( B ) spermine in the pipette solution. The eyes were intravitreally injected respectively with control-Fc (Ctr-Fc) and EphB2-Fc (2 μl, 1 μg/μl), and the recordings were made 5–7 d after the injections. The current amplitudes were all normalized to that of Ctr-Fc at −60 mV. C , Bar chart showing the average ratios of current amplitudes at +40/−60 mV in RGCs obtained from Ctr-Fc- and EphB2-Fc-injected retinae with or without spermine in the pipette solution. * p

    Techniques Used: Activation Assay, Transferring, Injection

    Intravitreal injection of PP2 reduces p-src and p-GluA2 levels in EphB2-Fc-injected retinae and retinae with COH. A , Representative immunoblots showing the changes in protein levels of p-src, src, and p-GluA2; and in levels of GluA2 in Ctr-Fc-injected retinae, EphB2-Fc-injected retinae, and retinae with COH (G2w), with or without the addition of PP2. B , Bar chart summarizing the average ratios of p-src/src and p-GluA2/GluA2 under different conditions. All of the data are normalized to control (Ctr-Fc). n = 6 for all the groups.
    Figure Legend Snippet: Intravitreal injection of PP2 reduces p-src and p-GluA2 levels in EphB2-Fc-injected retinae and retinae with COH. A , Representative immunoblots showing the changes in protein levels of p-src, src, and p-GluA2; and in levels of GluA2 in Ctr-Fc-injected retinae, EphB2-Fc-injected retinae, and retinae with COH (G2w), with or without the addition of PP2. B , Bar chart summarizing the average ratios of p-src/src and p-GluA2/GluA2 under different conditions. All of the data are normalized to control (Ctr-Fc). n = 6 for all the groups.

    Techniques Used: Injection, Western Blot

    Changes in membrane GluA2 protein expression in retinae with COH. A , Representative immunoblots showing the changes in GluA2 protein levels in the cell membrane component (m-GluA2) in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart summarizing the average densitometric quantification of immunoreactive bands of m-GluA2 at different times. All of the data are normalized to Ctr. n = 6. * p
    Figure Legend Snippet: Changes in membrane GluA2 protein expression in retinae with COH. A , Representative immunoblots showing the changes in GluA2 protein levels in the cell membrane component (m-GluA2) in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart summarizing the average densitometric quantification of immunoreactive bands of m-GluA2 at different times. All of the data are normalized to Ctr. n = 6. * p

    Techniques Used: Expressing, Western Blot

    Increase in p-GluA2 protein levels in COH rat retinae. A , Representative immunoblots showing the changes in p-GluA2 and GluA2 protein expression in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart shows that the average p-GluA2/GluA2 ratio steadily increased with postoperational time. All of the data are normalized to Ctr. n = 6 for all groups. * p
    Figure Legend Snippet: Increase in p-GluA2 protein levels in COH rat retinae. A , Representative immunoblots showing the changes in p-GluA2 and GluA2 protein expression in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart shows that the average p-GluA2/GluA2 ratio steadily increased with postoperational time. All of the data are normalized to Ctr. n = 6 for all groups. * p

    Techniques Used: Western Blot, Expressing

    2) Product Images from "GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model"

    Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.4376-14.2015

    Activation of EphB2/ephrinB2 reverse signaling induces GluA2-containing AMPA receptor trafficking. A , B , Representative traces showing the changes in amplitude of evoked EPSCs, mediated by AMPA receptors, recorded from four different RGCs in retinal slices at holding potentials of +40 and −60 mV with ( A ) or without ( B ) spermine in the pipette solution. The eyes were intravitreally injected respectively with control-Fc (Ctr-Fc) and EphB2-Fc (2 μl, 1 μg/μl), and the recordings were made 5–7 d after the injections. The current amplitudes were all normalized to that of Ctr-Fc at −60 mV. C , Bar chart showing the average ratios of current amplitudes at +40/−60 mV in RGCs obtained from Ctr-Fc- and EphB2-Fc-injected retinae with or without spermine in the pipette solution. * p
    Figure Legend Snippet: Activation of EphB2/ephrinB2 reverse signaling induces GluA2-containing AMPA receptor trafficking. A , B , Representative traces showing the changes in amplitude of evoked EPSCs, mediated by AMPA receptors, recorded from four different RGCs in retinal slices at holding potentials of +40 and −60 mV with ( A ) or without ( B ) spermine in the pipette solution. The eyes were intravitreally injected respectively with control-Fc (Ctr-Fc) and EphB2-Fc (2 μl, 1 μg/μl), and the recordings were made 5–7 d after the injections. The current amplitudes were all normalized to that of Ctr-Fc at −60 mV. C , Bar chart showing the average ratios of current amplitudes at +40/−60 mV in RGCs obtained from Ctr-Fc- and EphB2-Fc-injected retinae with or without spermine in the pipette solution. * p

    Techniques Used: Activation Assay, Transferring, Injection

    Intravitreal injection of PP2 reduces p-src and p-GluA2 levels in EphB2-Fc-injected retinae and retinae with COH. A , Representative immunoblots showing the changes in protein levels of p-src, src, and p-GluA2; and in levels of GluA2 in Ctr-Fc-injected retinae, EphB2-Fc-injected retinae, and retinae with COH (G2w), with or without the addition of PP2. B , Bar chart summarizing the average ratios of p-src/src and p-GluA2/GluA2 under different conditions. All of the data are normalized to control (Ctr-Fc). n = 6 for all the groups.
    Figure Legend Snippet: Intravitreal injection of PP2 reduces p-src and p-GluA2 levels in EphB2-Fc-injected retinae and retinae with COH. A , Representative immunoblots showing the changes in protein levels of p-src, src, and p-GluA2; and in levels of GluA2 in Ctr-Fc-injected retinae, EphB2-Fc-injected retinae, and retinae with COH (G2w), with or without the addition of PP2. B , Bar chart summarizing the average ratios of p-src/src and p-GluA2/GluA2 under different conditions. All of the data are normalized to control (Ctr-Fc). n = 6 for all the groups.

    Techniques Used: Injection, Western Blot

    Changes in membrane GluA2 protein expression in retinae with COH. A , Representative immunoblots showing the changes in GluA2 protein levels in the cell membrane component (m-GluA2) in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart summarizing the average densitometric quantification of immunoreactive bands of m-GluA2 at different times. All of the data are normalized to Ctr. n = 6. * p
    Figure Legend Snippet: Changes in membrane GluA2 protein expression in retinae with COH. A , Representative immunoblots showing the changes in GluA2 protein levels in the cell membrane component (m-GluA2) in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart summarizing the average densitometric quantification of immunoreactive bands of m-GluA2 at different times. All of the data are normalized to Ctr. n = 6. * p

    Techniques Used: Expressing, Western Blot

    Increase in p-GluA2 protein levels in COH rat retinae. A , Representative immunoblots showing the changes in p-GluA2 and GluA2 protein expression in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart shows that the average p-GluA2/GluA2 ratio steadily increased with postoperational time. All of the data are normalized to Ctr. n = 6 for all groups. * p
    Figure Legend Snippet: Increase in p-GluA2 protein levels in COH rat retinae. A , Representative immunoblots showing the changes in p-GluA2 and GluA2 protein expression in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart shows that the average p-GluA2/GluA2 ratio steadily increased with postoperational time. All of the data are normalized to Ctr. n = 6 for all groups. * p

    Techniques Used: Western Blot, Expressing

    3) Product Images from "GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model"

    Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.4376-14.2015

    Activation of EphB2/ephrinB2 reverse signaling induces GluA2-containing AMPA receptor trafficking. A , B , Representative traces showing the changes in amplitude of evoked EPSCs, mediated by AMPA receptors, recorded from four different RGCs in retinal slices at holding potentials of +40 and −60 mV with ( A ) or without ( B ) spermine in the pipette solution. The eyes were intravitreally injected respectively with control-Fc (Ctr-Fc) and EphB2-Fc (2 μl, 1 μg/μl), and the recordings were made 5–7 d after the injections. The current amplitudes were all normalized to that of Ctr-Fc at −60 mV. C , Bar chart showing the average ratios of current amplitudes at +40/−60 mV in RGCs obtained from Ctr-Fc- and EphB2-Fc-injected retinae with or without spermine in the pipette solution. * p
    Figure Legend Snippet: Activation of EphB2/ephrinB2 reverse signaling induces GluA2-containing AMPA receptor trafficking. A , B , Representative traces showing the changes in amplitude of evoked EPSCs, mediated by AMPA receptors, recorded from four different RGCs in retinal slices at holding potentials of +40 and −60 mV with ( A ) or without ( B ) spermine in the pipette solution. The eyes were intravitreally injected respectively with control-Fc (Ctr-Fc) and EphB2-Fc (2 μl, 1 μg/μl), and the recordings were made 5–7 d after the injections. The current amplitudes were all normalized to that of Ctr-Fc at −60 mV. C , Bar chart showing the average ratios of current amplitudes at +40/−60 mV in RGCs obtained from Ctr-Fc- and EphB2-Fc-injected retinae with or without spermine in the pipette solution. * p

    Techniques Used: Activation Assay, Transferring, Injection

    Intravitreal injection of PP2 reduces p-src and p-GluA2 levels in EphB2-Fc-injected retinae and retinae with COH. A , Representative immunoblots showing the changes in protein levels of p-src, src, and p-GluA2; and in levels of GluA2 in Ctr-Fc-injected retinae, EphB2-Fc-injected retinae, and retinae with COH (G2w), with or without the addition of PP2. B , Bar chart summarizing the average ratios of p-src/src and p-GluA2/GluA2 under different conditions. All of the data are normalized to control (Ctr-Fc). n = 6 for all the groups.
    Figure Legend Snippet: Intravitreal injection of PP2 reduces p-src and p-GluA2 levels in EphB2-Fc-injected retinae and retinae with COH. A , Representative immunoblots showing the changes in protein levels of p-src, src, and p-GluA2; and in levels of GluA2 in Ctr-Fc-injected retinae, EphB2-Fc-injected retinae, and retinae with COH (G2w), with or without the addition of PP2. B , Bar chart summarizing the average ratios of p-src/src and p-GluA2/GluA2 under different conditions. All of the data are normalized to control (Ctr-Fc). n = 6 for all the groups.

    Techniques Used: Injection, Western Blot

    Changes in membrane GluA2 protein expression in retinae with COH. A , Representative immunoblots showing the changes in GluA2 protein levels in the cell membrane component (m-GluA2) in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart summarizing the average densitometric quantification of immunoreactive bands of m-GluA2 at different times. All of the data are normalized to Ctr. n = 6. * p
    Figure Legend Snippet: Changes in membrane GluA2 protein expression in retinae with COH. A , Representative immunoblots showing the changes in GluA2 protein levels in the cell membrane component (m-GluA2) in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart summarizing the average densitometric quantification of immunoreactive bands of m-GluA2 at different times. All of the data are normalized to Ctr. n = 6. * p

    Techniques Used: Expressing, Western Blot

    Increase in p-GluA2 protein levels in COH rat retinae. A , Representative immunoblots showing the changes in p-GluA2 and GluA2 protein expression in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart shows that the average p-GluA2/GluA2 ratio steadily increased with postoperational time. All of the data are normalized to Ctr. n = 6 for all groups. * p
    Figure Legend Snippet: Increase in p-GluA2 protein levels in COH rat retinae. A , Representative immunoblots showing the changes in p-GluA2 and GluA2 protein expression in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart shows that the average p-GluA2/GluA2 ratio steadily increased with postoperational time. All of the data are normalized to Ctr. n = 6 for all groups. * p

    Techniques Used: Western Blot, Expressing

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    Alomone Labs polyclonal rabbit anti glua2
    Activation of EphB2/ephrinB2 reverse signaling induces <t>GluA2-containing</t> AMPA receptor trafficking. A , B , Representative traces showing the changes in amplitude of evoked EPSCs, mediated by AMPA receptors, recorded from four different RGCs in retinal slices at holding potentials of +40 and −60 mV with ( A ) or without ( B ) spermine in the pipette solution. The eyes were intravitreally injected respectively with control-Fc (Ctr-Fc) and EphB2-Fc (2 μl, 1 μg/μl), and the recordings were made 5–7 d after the injections. The current amplitudes were all normalized to that of Ctr-Fc at −60 mV. C , Bar chart showing the average ratios of current amplitudes at +40/−60 mV in RGCs obtained from Ctr-Fc- and EphB2-Fc-injected retinae with or without spermine in the pipette solution. * p
    Polyclonal Rabbit Anti Glua2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti glua2/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti glua2 - by Bioz Stars, 2022-05
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Activation of EphB2/ephrinB2 reverse signaling induces GluA2-containing AMPA receptor trafficking. A , B , Representative traces showing the changes in amplitude of evoked EPSCs, mediated by AMPA receptors, recorded from four different RGCs in retinal slices at holding potentials of +40 and −60 mV with ( A ) or without ( B ) spermine in the pipette solution. The eyes were intravitreally injected respectively with control-Fc (Ctr-Fc) and EphB2-Fc (2 μl, 1 μg/μl), and the recordings were made 5–7 d after the injections. The current amplitudes were all normalized to that of Ctr-Fc at −60 mV. C , Bar chart showing the average ratios of current amplitudes at +40/−60 mV in RGCs obtained from Ctr-Fc- and EphB2-Fc-injected retinae with or without spermine in the pipette solution. * p

    Journal: The Journal of Neuroscience

    Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model

    doi: 10.1523/JNEUROSCI.4376-14.2015

    Figure Lengend Snippet: Activation of EphB2/ephrinB2 reverse signaling induces GluA2-containing AMPA receptor trafficking. A , B , Representative traces showing the changes in amplitude of evoked EPSCs, mediated by AMPA receptors, recorded from four different RGCs in retinal slices at holding potentials of +40 and −60 mV with ( A ) or without ( B ) spermine in the pipette solution. The eyes were intravitreally injected respectively with control-Fc (Ctr-Fc) and EphB2-Fc (2 μl, 1 μg/μl), and the recordings were made 5–7 d after the injections. The current amplitudes were all normalized to that of Ctr-Fc at −60 mV. C , Bar chart showing the average ratios of current amplitudes at +40/−60 mV in RGCs obtained from Ctr-Fc- and EphB2-Fc-injected retinae with or without spermine in the pipette solution. * p

    Article Snippet: After blocking in 5% nonfat milk (for a nonphosphorylated antibody) or 5% bovine serum albumin (for a phosphorylated antibody) at room temperature for 1.5 h, the membranes were incubated overnight at 4°C with the following primary antibodies: monoclonal mouse anti-β-actin (1:3000 dilution; Sigma-Aldrich), monoclonal mouse anti-EphB1 (1:300 dilution; R & D Systems), polyclonal goat anti-EphB2 (1:500 dilution; R & D Systems), polyclonal goat anti-ephrinB1 (1:500 dilution; R & D Systems), polyclonal goat anti-ephrinB2 (1:500 dilution; R & D Systems), polyclonal rabbit anti-phospho-ephrinB (Tyr 314; 1:1000 dilution; R & D Systems), polyclonal rabbit anti-src (1:1000 dilution; Cell Signaling Technology), polyclonal rabbit anti-phospho-src (Tyr 418; Sigma-Aldrich), polyclonal rabbit anti-GluA2 (1:200 dilution; Alomone Labs), polyclonal rabbit anti-phospho-GluA2 (Tyr 876; 1:200 dilution; Cell Signaling Technology).

    Techniques: Activation Assay, Transferring, Injection

    Intravitreal injection of PP2 reduces p-src and p-GluA2 levels in EphB2-Fc-injected retinae and retinae with COH. A , Representative immunoblots showing the changes in protein levels of p-src, src, and p-GluA2; and in levels of GluA2 in Ctr-Fc-injected retinae, EphB2-Fc-injected retinae, and retinae with COH (G2w), with or without the addition of PP2. B , Bar chart summarizing the average ratios of p-src/src and p-GluA2/GluA2 under different conditions. All of the data are normalized to control (Ctr-Fc). n = 6 for all the groups.

    Journal: The Journal of Neuroscience

    Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model

    doi: 10.1523/JNEUROSCI.4376-14.2015

    Figure Lengend Snippet: Intravitreal injection of PP2 reduces p-src and p-GluA2 levels in EphB2-Fc-injected retinae and retinae with COH. A , Representative immunoblots showing the changes in protein levels of p-src, src, and p-GluA2; and in levels of GluA2 in Ctr-Fc-injected retinae, EphB2-Fc-injected retinae, and retinae with COH (G2w), with or without the addition of PP2. B , Bar chart summarizing the average ratios of p-src/src and p-GluA2/GluA2 under different conditions. All of the data are normalized to control (Ctr-Fc). n = 6 for all the groups.

    Article Snippet: After blocking in 5% nonfat milk (for a nonphosphorylated antibody) or 5% bovine serum albumin (for a phosphorylated antibody) at room temperature for 1.5 h, the membranes were incubated overnight at 4°C with the following primary antibodies: monoclonal mouse anti-β-actin (1:3000 dilution; Sigma-Aldrich), monoclonal mouse anti-EphB1 (1:300 dilution; R & D Systems), polyclonal goat anti-EphB2 (1:500 dilution; R & D Systems), polyclonal goat anti-ephrinB1 (1:500 dilution; R & D Systems), polyclonal goat anti-ephrinB2 (1:500 dilution; R & D Systems), polyclonal rabbit anti-phospho-ephrinB (Tyr 314; 1:1000 dilution; R & D Systems), polyclonal rabbit anti-src (1:1000 dilution; Cell Signaling Technology), polyclonal rabbit anti-phospho-src (Tyr 418; Sigma-Aldrich), polyclonal rabbit anti-GluA2 (1:200 dilution; Alomone Labs), polyclonal rabbit anti-phospho-GluA2 (Tyr 876; 1:200 dilution; Cell Signaling Technology).

    Techniques: Injection, Western Blot

    Changes in membrane GluA2 protein expression in retinae with COH. A , Representative immunoblots showing the changes in GluA2 protein levels in the cell membrane component (m-GluA2) in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart summarizing the average densitometric quantification of immunoreactive bands of m-GluA2 at different times. All of the data are normalized to Ctr. n = 6. * p

    Journal: The Journal of Neuroscience

    Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model

    doi: 10.1523/JNEUROSCI.4376-14.2015

    Figure Lengend Snippet: Changes in membrane GluA2 protein expression in retinae with COH. A , Representative immunoblots showing the changes in GluA2 protein levels in the cell membrane component (m-GluA2) in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart summarizing the average densitometric quantification of immunoreactive bands of m-GluA2 at different times. All of the data are normalized to Ctr. n = 6. * p

    Article Snippet: After blocking in 5% nonfat milk (for a nonphosphorylated antibody) or 5% bovine serum albumin (for a phosphorylated antibody) at room temperature for 1.5 h, the membranes were incubated overnight at 4°C with the following primary antibodies: monoclonal mouse anti-β-actin (1:3000 dilution; Sigma-Aldrich), monoclonal mouse anti-EphB1 (1:300 dilution; R & D Systems), polyclonal goat anti-EphB2 (1:500 dilution; R & D Systems), polyclonal goat anti-ephrinB1 (1:500 dilution; R & D Systems), polyclonal goat anti-ephrinB2 (1:500 dilution; R & D Systems), polyclonal rabbit anti-phospho-ephrinB (Tyr 314; 1:1000 dilution; R & D Systems), polyclonal rabbit anti-src (1:1000 dilution; Cell Signaling Technology), polyclonal rabbit anti-phospho-src (Tyr 418; Sigma-Aldrich), polyclonal rabbit anti-GluA2 (1:200 dilution; Alomone Labs), polyclonal rabbit anti-phospho-GluA2 (Tyr 876; 1:200 dilution; Cell Signaling Technology).

    Techniques: Expressing, Western Blot

    Increase in p-GluA2 protein levels in COH rat retinae. A , Representative immunoblots showing the changes in p-GluA2 and GluA2 protein expression in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart shows that the average p-GluA2/GluA2 ratio steadily increased with postoperational time. All of the data are normalized to Ctr. n = 6 for all groups. * p

    Journal: The Journal of Neuroscience

    Article Title: GluA2 Trafficking Is Involved in Apoptosis of Retinal Ganglion Cells Induced by Activation of EphB/EphrinB Reverse Signaling in a Rat Chronic Ocular Hypertension Model

    doi: 10.1523/JNEUROSCI.4376-14.2015

    Figure Lengend Snippet: Increase in p-GluA2 protein levels in COH rat retinae. A , Representative immunoblots showing the changes in p-GluA2 and GluA2 protein expression in sham-operated (Ctr) and COH retinal extracts at different postoperational times (G1d, G3d, G1w, G2w, G3w, G4w, and G6w). B , Bar chart shows that the average p-GluA2/GluA2 ratio steadily increased with postoperational time. All of the data are normalized to Ctr. n = 6 for all groups. * p

    Article Snippet: After blocking in 5% nonfat milk (for a nonphosphorylated antibody) or 5% bovine serum albumin (for a phosphorylated antibody) at room temperature for 1.5 h, the membranes were incubated overnight at 4°C with the following primary antibodies: monoclonal mouse anti-β-actin (1:3000 dilution; Sigma-Aldrich), monoclonal mouse anti-EphB1 (1:300 dilution; R & D Systems), polyclonal goat anti-EphB2 (1:500 dilution; R & D Systems), polyclonal goat anti-ephrinB1 (1:500 dilution; R & D Systems), polyclonal goat anti-ephrinB2 (1:500 dilution; R & D Systems), polyclonal rabbit anti-phospho-ephrinB (Tyr 314; 1:1000 dilution; R & D Systems), polyclonal rabbit anti-src (1:1000 dilution; Cell Signaling Technology), polyclonal rabbit anti-phospho-src (Tyr 418; Sigma-Aldrich), polyclonal rabbit anti-GluA2 (1:200 dilution; Alomone Labs), polyclonal rabbit anti-phospho-GluA2 (Tyr 876; 1:200 dilution; Cell Signaling Technology).

    Techniques: Western Blot, Expressing