bsa  (Alomone Labs)


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    Structured Review

    Alomone Labs bsa
    Functional NMDA receptors are localized on the surface of HPASMCs. Immunofluorescence staining was performed on non-permeabilized HPASMCs. Both GluN1 and GluN2 (2B and 2D) subunits were detected on the surface of HPASMCs ( A , B ). GluN1 co-localized with <t>GluN2B</t> ( A ) and GluN2D ( B ) on the surface of HPASMCs, respectively. Results are representative images taken from at least three separate experiments. Scale bar = 100 µM. ( C , D ) are representative scatter plots of each fluorescent pixel from confocal images with GluN1 green fluorescent intensity along the x -axis and GluN2 red fluorescent intensity along the y -axis. The shaded area in the upper right quadrant represents colocalized pixels above background with the associated Pearson correlation coefficient indicated for all colocalized pixels. ( E ) Representative traces for cultured HPASMCs in response to 100 μM NMDA and 10 μM glycine treatment. Cells were clamped at − 50 mV and whole cell recordings were performed. ( F ) NMDA and glycine treatment evoked an inward whole-cell current of 10.9 ± 1.7 pA (Mean ± SE, **p
    Bsa, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsa/product/Alomone Labs
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    bsa - by Bioz Stars, 2022-12
    93/100 stars

    Images

    1) Product Images from "Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells"

    Article Title: Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-87667-0

    Functional NMDA receptors are localized on the surface of HPASMCs. Immunofluorescence staining was performed on non-permeabilized HPASMCs. Both GluN1 and GluN2 (2B and 2D) subunits were detected on the surface of HPASMCs ( A , B ). GluN1 co-localized with GluN2B ( A ) and GluN2D ( B ) on the surface of HPASMCs, respectively. Results are representative images taken from at least three separate experiments. Scale bar = 100 µM. ( C , D ) are representative scatter plots of each fluorescent pixel from confocal images with GluN1 green fluorescent intensity along the x -axis and GluN2 red fluorescent intensity along the y -axis. The shaded area in the upper right quadrant represents colocalized pixels above background with the associated Pearson correlation coefficient indicated for all colocalized pixels. ( E ) Representative traces for cultured HPASMCs in response to 100 μM NMDA and 10 μM glycine treatment. Cells were clamped at − 50 mV and whole cell recordings were performed. ( F ) NMDA and glycine treatment evoked an inward whole-cell current of 10.9 ± 1.7 pA (Mean ± SE, **p
    Figure Legend Snippet: Functional NMDA receptors are localized on the surface of HPASMCs. Immunofluorescence staining was performed on non-permeabilized HPASMCs. Both GluN1 and GluN2 (2B and 2D) subunits were detected on the surface of HPASMCs ( A , B ). GluN1 co-localized with GluN2B ( A ) and GluN2D ( B ) on the surface of HPASMCs, respectively. Results are representative images taken from at least three separate experiments. Scale bar = 100 µM. ( C , D ) are representative scatter plots of each fluorescent pixel from confocal images with GluN1 green fluorescent intensity along the x -axis and GluN2 red fluorescent intensity along the y -axis. The shaded area in the upper right quadrant represents colocalized pixels above background with the associated Pearson correlation coefficient indicated for all colocalized pixels. ( E ) Representative traces for cultured HPASMCs in response to 100 μM NMDA and 10 μM glycine treatment. Cells were clamped at − 50 mV and whole cell recordings were performed. ( F ) NMDA and glycine treatment evoked an inward whole-cell current of 10.9 ± 1.7 pA (Mean ± SE, **p

    Techniques Used: Functional Assay, Immunofluorescence, Staining, Cell Culture

    2) Product Images from "Cholesterol modulates presynaptic and postsynaptic properties of excitatory synaptic transmission"

    Article Title: Cholesterol modulates presynaptic and postsynaptic properties of excitatory synaptic transmission

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-69454-5

    Cholesterol depletion reduces synaptic localization of NMDARs. ( A , C ) Colocalization of surface GluN2A (A, green) or GluN2B (C, green) and the postsynaptic marker Shank (red) in control and cholesterol-depleted neurons (10 mM MβCD pretreatment, 5 min). Scale bar 2 µm. ( B , D ) Bar graphs showing Pearson's coefficient for the colocalization indicate the reduction of synaptic localization of GluN2A and GluN2B after cholesterol depletion. ( E ) Colocalization of surface GluA1 (green) and the postsynaptic marker Shank (red) in control and cholesterol-depleted neurons (MβCD). Scale bar 2 µm. ( F ) Bar graph showing Pearson's coefficient for the colocalization. ( G ) Examples of typical dual AMPAR-NMDAR mEPSCs in control autaptic neurons having various AMPAR to NMDAR ratio. ( H ) Examples of typical dual AMPAR-NMDAR mEPSCs in 10 mM MβCD-pretreated autaptic neurons. ( I ) Examples of NMDAR mEPSCs obtained from average dual mEPSCs after AMPAR mEPSC subtraction. A control neuron (top trace) and a cholesterol-depleted neuron (bottom trace). The arrows indicate mEPSC onsets. ( J ) The comparison of average amplitude of NMDAR mEPSCs in control neurons and in cholesterol-depleted neurons. (* p
    Figure Legend Snippet: Cholesterol depletion reduces synaptic localization of NMDARs. ( A , C ) Colocalization of surface GluN2A (A, green) or GluN2B (C, green) and the postsynaptic marker Shank (red) in control and cholesterol-depleted neurons (10 mM MβCD pretreatment, 5 min). Scale bar 2 µm. ( B , D ) Bar graphs showing Pearson's coefficient for the colocalization indicate the reduction of synaptic localization of GluN2A and GluN2B after cholesterol depletion. ( E ) Colocalization of surface GluA1 (green) and the postsynaptic marker Shank (red) in control and cholesterol-depleted neurons (MβCD). Scale bar 2 µm. ( F ) Bar graph showing Pearson's coefficient for the colocalization. ( G ) Examples of typical dual AMPAR-NMDAR mEPSCs in control autaptic neurons having various AMPAR to NMDAR ratio. ( H ) Examples of typical dual AMPAR-NMDAR mEPSCs in 10 mM MβCD-pretreated autaptic neurons. ( I ) Examples of NMDAR mEPSCs obtained from average dual mEPSCs after AMPAR mEPSC subtraction. A control neuron (top trace) and a cholesterol-depleted neuron (bottom trace). The arrows indicate mEPSC onsets. ( J ) The comparison of average amplitude of NMDAR mEPSCs in control neurons and in cholesterol-depleted neurons. (* p

    Techniques Used: Marker

    Cholesterol depletion reduces the fraction of synaptic immobile NMDARs. ( A ) Surface NMDARs were detected using a QD-antibody complex directed against extracellular epitopes in GluN2A or GluN2B. Left, representative summed trajectories of NMDAR-QDs (red) acquired over a period of 25 s (20 Hz frame rate) in hippocampal neurons. Scale bar 5 µm. Right, representative examples of NMDAR reconstructed trajectories. ( B , C ) Diffusion coefficients for synaptic GluN2A-containing NMDARs and GluN2B-containing NMDARs in control and after cholesterol depletion (10 mM MβCD pretreatment, 5 min). ( D , E ) Diffusion coefficients for extrasynaptic GluN2A-containing NMDAR and GluN2B-containing NMDARs in control and after cholesterol depletion. ( F , G ) Diffusion coefficients for the mobile fraction of synaptic GluN2A and GluN2B-containing NMDARs in control and after cholesterol depletion. ( H , I ) Fraction of synaptic immobile receptors in control and after cholesterol depletion. (* p
    Figure Legend Snippet: Cholesterol depletion reduces the fraction of synaptic immobile NMDARs. ( A ) Surface NMDARs were detected using a QD-antibody complex directed against extracellular epitopes in GluN2A or GluN2B. Left, representative summed trajectories of NMDAR-QDs (red) acquired over a period of 25 s (20 Hz frame rate) in hippocampal neurons. Scale bar 5 µm. Right, representative examples of NMDAR reconstructed trajectories. ( B , C ) Diffusion coefficients for synaptic GluN2A-containing NMDARs and GluN2B-containing NMDARs in control and after cholesterol depletion (10 mM MβCD pretreatment, 5 min). ( D , E ) Diffusion coefficients for extrasynaptic GluN2A-containing NMDAR and GluN2B-containing NMDARs in control and after cholesterol depletion. ( F , G ) Diffusion coefficients for the mobile fraction of synaptic GluN2A and GluN2B-containing NMDARs in control and after cholesterol depletion. ( H , I ) Fraction of synaptic immobile receptors in control and after cholesterol depletion. (* p

    Techniques Used: Diffusion-based Assay

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    Alomone Labs rabbit anti nr2b
    Rabbit Anti Nr2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Alomone Labs glun2b rabbit anti glun2b
    Distribution of <t>GluN2B</t> immunoreactivity in young and aged GIN mice hippocampus. Panoramic confocal plane showing the distribution of O-LM cells (green) and GluN2B immunoreactivity (red) in the hippocampus of 3-month-old (A1) and 16-month-old (B1) mice. Different regions and strata are indicated with dotted lines. (A2,B2) High magnification view from the different CA1 strata in 3-month-old (A2) and 16-month-old (B2) mice. (A3,B3) Enlarged view of the squared regions in panels (A2,B2) , showing double immunofluorescence for GFP/GluN2B, in strata oriens , and pyramidale. Note the presence of GluN2B + clusters in pyramidal neurons in 16-month-old (B3) , but not in 3-month-old (A3) mice. Scale bar: 150 μm for panels (A1,B1 ), 67 μm for panels (A2,B2) , and 21 μm for panels (A3,B3) .
    Glun2b Rabbit Anti Glun2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Distribution of GluN2B immunoreactivity in young and aged GIN mice hippocampus. Panoramic confocal plane showing the distribution of O-LM cells (green) and GluN2B immunoreactivity (red) in the hippocampus of 3-month-old (A1) and 16-month-old (B1) mice. Different regions and strata are indicated with dotted lines. (A2,B2) High magnification view from the different CA1 strata in 3-month-old (A2) and 16-month-old (B2) mice. (A3,B3) Enlarged view of the squared regions in panels (A2,B2) , showing double immunofluorescence for GFP/GluN2B, in strata oriens , and pyramidale. Note the presence of GluN2B + clusters in pyramidal neurons in 16-month-old (B3) , but not in 3-month-old (A3) mice. Scale bar: 150 μm for panels (A1,B1 ), 67 μm for panels (A2,B2) , and 21 μm for panels (A3,B3) .

    Journal: Frontiers in Aging Neuroscience

    Article Title: Effects of Aging on the Structure and Expression of NMDA Receptors of Somatostatin Expressing Neurons in the Mouse Hippocampus

    doi: 10.3389/fnagi.2021.782737

    Figure Lengend Snippet: Distribution of GluN2B immunoreactivity in young and aged GIN mice hippocampus. Panoramic confocal plane showing the distribution of O-LM cells (green) and GluN2B immunoreactivity (red) in the hippocampus of 3-month-old (A1) and 16-month-old (B1) mice. Different regions and strata are indicated with dotted lines. (A2,B2) High magnification view from the different CA1 strata in 3-month-old (A2) and 16-month-old (B2) mice. (A3,B3) Enlarged view of the squared regions in panels (A2,B2) , showing double immunofluorescence for GFP/GluN2B, in strata oriens , and pyramidale. Note the presence of GluN2B + clusters in pyramidal neurons in 16-month-old (B3) , but not in 3-month-old (A3) mice. Scale bar: 150 μm for panels (A1,B1 ), 67 μm for panels (A2,B2) , and 21 μm for panels (A3,B3) .

    Article Snippet: In order to study the GluN1 expression, sections were incubated with rabbit anti-GluN1 (Alomone, 1:400) or GluN2B rabbit anti-GluN2B (Alomone, 1:4000) together with chicken anti-GFP IgY (Abcam, 1:500) primary antibodies for 48 h at 4°C.

    Techniques: Mouse Assay, Immunofluorescence

    Analysis of the density and percentage of area covered with GluN2B immunoreactive puncta in the somata and in the periphery of O-LM cells during aging. (A–F) Double GFP/GluN2B immunohistochemistry in 3-month-old (A) , 9-month-old (B) , 16-month-old (C) female mice and in 3-month-old (D) , 9-month-old (E) , 16-month-old (F) male mice. In panels (C2,F2) , a detailed view of the GluN2B clustering in aged mice can be observed. (G–I) Graphs showing the density and percentage of area covered with GluN2B immunoreactive puncta in the somata (G1,G2–I2) and in its periphery (G3,G4–I4) in animals segregated by sex (G1–4) , pooled females (H1–4) and males (I1–4) (all graphs represent mean ± SEM., * p -value

    Journal: Frontiers in Aging Neuroscience

    Article Title: Effects of Aging on the Structure and Expression of NMDA Receptors of Somatostatin Expressing Neurons in the Mouse Hippocampus

    doi: 10.3389/fnagi.2021.782737

    Figure Lengend Snippet: Analysis of the density and percentage of area covered with GluN2B immunoreactive puncta in the somata and in the periphery of O-LM cells during aging. (A–F) Double GFP/GluN2B immunohistochemistry in 3-month-old (A) , 9-month-old (B) , 16-month-old (C) female mice and in 3-month-old (D) , 9-month-old (E) , 16-month-old (F) male mice. In panels (C2,F2) , a detailed view of the GluN2B clustering in aged mice can be observed. (G–I) Graphs showing the density and percentage of area covered with GluN2B immunoreactive puncta in the somata (G1,G2–I2) and in its periphery (G3,G4–I4) in animals segregated by sex (G1–4) , pooled females (H1–4) and males (I1–4) (all graphs represent mean ± SEM., * p -value

    Article Snippet: In order to study the GluN1 expression, sections were incubated with rabbit anti-GluN1 (Alomone, 1:400) or GluN2B rabbit anti-GluN2B (Alomone, 1:4000) together with chicken anti-GFP IgY (Abcam, 1:500) primary antibodies for 48 h at 4°C.

    Techniques: Immunohistochemistry, Mouse Assay

    Age-dependent calcium permeable channel expressions in the piriform cortex. ( A ) Synaptic expressions of L-type calcium channel Cav1.2 subunit. Neonate: n = 6; Adult: n = 7, Aged: n = 9. ( B ) Extra-synaptic expressions of Cav1.2 subunit. Neonate: n = 6; Adult: n = 8, Aged: n = 9. ( C ) Synaptic expressions of NMDAR GluN1 subunit. Neonate: n = 9; Adult: n = 10, Aged: n = 11. ( D ) Extra-synaptic expressions of GluN1 subunit. Neonate: n = 8; Adult: n = 11, Aged: n = 10. ( E ) Synaptic expressions of NMDAR GluN2A subunit. Neonate: n = 9; Adult: n = 10, Aged: n = 10. ( F ) Extra-synaptic expressions of GluN2A subunit. Neonate: n = 9; Adult: n = 12, Aged: n = 10. ( G ) Synaptic expressions of NMDAR GluN2B subunit. Neonate: n = 9; Adult: n = 10, Aged: n = 11. ( H ) Extra-synaptic expressions of GluN2B subunit. Neonate: n = 10; Adult: n = 10, Aged: n = 11. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Age-Dependent Contributions of NMDA Receptors and L-Type Calcium Channels to Long-Term Depression in the Piriform Cortex

    doi: 10.3390/ijms222413551

    Figure Lengend Snippet: Age-dependent calcium permeable channel expressions in the piriform cortex. ( A ) Synaptic expressions of L-type calcium channel Cav1.2 subunit. Neonate: n = 6; Adult: n = 7, Aged: n = 9. ( B ) Extra-synaptic expressions of Cav1.2 subunit. Neonate: n = 6; Adult: n = 8, Aged: n = 9. ( C ) Synaptic expressions of NMDAR GluN1 subunit. Neonate: n = 9; Adult: n = 10, Aged: n = 11. ( D ) Extra-synaptic expressions of GluN1 subunit. Neonate: n = 8; Adult: n = 11, Aged: n = 10. ( E ) Synaptic expressions of NMDAR GluN2A subunit. Neonate: n = 9; Adult: n = 10, Aged: n = 10. ( F ) Extra-synaptic expressions of GluN2A subunit. Neonate: n = 9; Adult: n = 12, Aged: n = 10. ( G ) Synaptic expressions of NMDAR GluN2B subunit. Neonate: n = 9; Adult: n = 10, Aged: n = 11. ( H ) Extra-synaptic expressions of GluN2B subunit. Neonate: n = 10; Adult: n = 10, Aged: n = 11. * p

    Article Snippet: A primary antibody (FP1 rabbit Anti-Cav1.2 antibody (1:500) [ ], or Anti-GluN2B (1:2000; cat. no. AGC-003; Alomone Labs) was applied in 5% BSA in Tris buffer on shaker at 4 °C overnight.

    Techniques:

    The role of GluN2B in age-dependent LTD in the piriform layer Ib. ( A ) Example traces and time courses of fEPSPs with LTD inductions in the presence of Ro25-6981 (1 µM) in neonate and adult rats. Scale bars, 0.5 mV, 5 msec. ( B ) % changes of fEPSPs at 30 min post-LTD induction. Neonate: n = 8; Adult: n = 9.

    Journal: International Journal of Molecular Sciences

    Article Title: Age-Dependent Contributions of NMDA Receptors and L-Type Calcium Channels to Long-Term Depression in the Piriform Cortex

    doi: 10.3390/ijms222413551

    Figure Lengend Snippet: The role of GluN2B in age-dependent LTD in the piriform layer Ib. ( A ) Example traces and time courses of fEPSPs with LTD inductions in the presence of Ro25-6981 (1 µM) in neonate and adult rats. Scale bars, 0.5 mV, 5 msec. ( B ) % changes of fEPSPs at 30 min post-LTD induction. Neonate: n = 8; Adult: n = 9.

    Article Snippet: A primary antibody (FP1 rabbit Anti-Cav1.2 antibody (1:500) [ ], or Anti-GluN2B (1:2000; cat. no. AGC-003; Alomone Labs) was applied in 5% BSA in Tris buffer on shaker at 4 °C overnight.

    Techniques:

    Age-dependent Cav1.2 and GluN2B expressions in the piriform cortex layer Ib. ( A1 ) Example staining of Cav1.2 and PSD95 in neonate, adult and aged PC layer Ib. Scale bars, 5 µm. ( A2 ) Puncta numbers of Cav1.2 and PSD95. ( A3 ) Normalized punta numbers of Cav1.2 over PSD95. ( B1 ) Example staining of GluN2B and PSD95 in neonate, adult and aged PC layer Ib. Scale bars, 5 µm. ( B2 ) Puncta numbers of GluN2B and PSD95. ( B3 ) Normalized punta numbers of GluN2B over PSD95. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Age-Dependent Contributions of NMDA Receptors and L-Type Calcium Channels to Long-Term Depression in the Piriform Cortex

    doi: 10.3390/ijms222413551

    Figure Lengend Snippet: Age-dependent Cav1.2 and GluN2B expressions in the piriform cortex layer Ib. ( A1 ) Example staining of Cav1.2 and PSD95 in neonate, adult and aged PC layer Ib. Scale bars, 5 µm. ( A2 ) Puncta numbers of Cav1.2 and PSD95. ( A3 ) Normalized punta numbers of Cav1.2 over PSD95. ( B1 ) Example staining of GluN2B and PSD95 in neonate, adult and aged PC layer Ib. Scale bars, 5 µm. ( B2 ) Puncta numbers of GluN2B and PSD95. ( B3 ) Normalized punta numbers of GluN2B over PSD95. * p

    Article Snippet: A primary antibody (FP1 rabbit Anti-Cav1.2 antibody (1:500) [ ], or Anti-GluN2B (1:2000; cat. no. AGC-003; Alomone Labs) was applied in 5% BSA in Tris buffer on shaker at 4 °C overnight.

    Techniques: Staining