Journal: bioRxiv

Article Title: The two-component system YesMN promotes pneumococcal host-to-host transmission, and regulates genes involved in zinc homeostasis

doi: 10.1101/2022.08.29.505782

Figure Lengend Snippet: Comparison of level binding of T4S, the ΔyesMN mutant and the yesMN + strain to immobilized human nasal fluid (hNF). Bacteria (2x 10 4 CFU/100 μl DMEM) were incubated with 100 μl of 10 µg of immobilized hNF in presence of 0.1% BSA for 2hr at 30°C. After 19 washes, adherent bacteria were determined by resuspending with 0.001% Triton X-100 following plating on TSY agar plates supplemented with 200 μg/ml streptomycin. Values are means of three independent determinations in triplicate ± SEM. Statistical differences were calculated using the Kruskal-Wallis analysis of variance with Dunn’s posttest. **, P < 0.01; ***, P < 0.001; n.s, not significant.

Article Snippet: To quantitate S. pneumoniae , serial dilutions were routinely plated on TSY agar-streptomycin (200 µg/ml), supplemented with either catalase (6,300 U/plate; Worthington Biochemical Corporation) or 5% defibrinated sheep’s blood, and incubated overnight at 37°C with 5% CO 2 .

Techniques: Binding Assay, Mutagenesis, Incubation