anti gaba a r α5  (Alomone Labs)


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    Alomone Labs anti gaba a r α5
    Protein levels of the main GABAergic and glutamatergic receptors in 6 month old male hAPP wt mice. ( a ) Quantification and representative western blots of GABAergic receptor protein levels, including GABA B R and <t>GABA</t> <t>A</t> <t>R</t> α1, α2, α3 and <t>α5</t> subunits, measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. ( b ) Quantification and representative western blots of glutamatergic receptor subunits of NMDA receptor (GluN2A, GluN2B) and AMPA receptor (GluA1, GluA2) measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. Protein amount was normalized to β-tubulin and expressed as percentage (Values are means ± SEM; *P ≤ 0.05, two-tailed unpaired t-test with Welch’s correction).
    Anti Gaba A R α5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gaba a r α5/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gaba a r α5 - by Bioz Stars, 2023-01
    93/100 stars

    Images

    1) Product Images from "Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission"

    Article Title: Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-97144-3

    Protein levels of the main GABAergic and glutamatergic receptors in 6 month old male hAPP wt mice. ( a ) Quantification and representative western blots of GABAergic receptor protein levels, including GABA B R and GABA A R α1, α2, α3 and α5 subunits, measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. ( b ) Quantification and representative western blots of glutamatergic receptor subunits of NMDA receptor (GluN2A, GluN2B) and AMPA receptor (GluA1, GluA2) measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. Protein amount was normalized to β-tubulin and expressed as percentage (Values are means ± SEM; *P ≤ 0.05, two-tailed unpaired t-test with Welch’s correction).
    Figure Legend Snippet: Protein levels of the main GABAergic and glutamatergic receptors in 6 month old male hAPP wt mice. ( a ) Quantification and representative western blots of GABAergic receptor protein levels, including GABA B R and GABA A R α1, α2, α3 and α5 subunits, measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. ( b ) Quantification and representative western blots of glutamatergic receptor subunits of NMDA receptor (GluN2A, GluN2B) and AMPA receptor (GluA1, GluA2) measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. Protein amount was normalized to β-tubulin and expressed as percentage (Values are means ± SEM; *P ≤ 0.05, two-tailed unpaired t-test with Welch’s correction).

    Techniques Used: Western Blot, Two Tailed Test

    anti gaba a r α5  (Alomone Labs)


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    Structured Review

    Alomone Labs anti gaba a r α5
    Protein levels of the main GABAergic and glutamatergic receptors in 6 month old male hAPP wt mice. ( a ) Quantification and representative western blots of GABAergic receptor protein levels, including GABA B R and <t>GABA</t> <t>A</t> <t>R</t> α1, α2, α3 and <t>α5</t> subunits, measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. ( b ) Quantification and representative western blots of glutamatergic receptor subunits of NMDA receptor (GluN2A, GluN2B) and AMPA receptor (GluA1, GluA2) measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. Protein amount was normalized to β-tubulin and expressed as percentage (Values are means ± SEM; *P ≤ 0.05, two-tailed unpaired t-test with Welch’s correction).
    Anti Gaba A R α5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gaba a r α5/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gaba a r α5 - by Bioz Stars, 2023-01
    93/100 stars

    Images

    1) Product Images from "Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission"

    Article Title: Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-97144-3

    Protein levels of the main GABAergic and glutamatergic receptors in 6 month old male hAPP wt mice. ( a ) Quantification and representative western blots of GABAergic receptor protein levels, including GABA B R and GABA A R α1, α2, α3 and α5 subunits, measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. ( b ) Quantification and representative western blots of glutamatergic receptor subunits of NMDA receptor (GluN2A, GluN2B) and AMPA receptor (GluA1, GluA2) measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. Protein amount was normalized to β-tubulin and expressed as percentage (Values are means ± SEM; *P ≤ 0.05, two-tailed unpaired t-test with Welch’s correction).
    Figure Legend Snippet: Protein levels of the main GABAergic and glutamatergic receptors in 6 month old male hAPP wt mice. ( a ) Quantification and representative western blots of GABAergic receptor protein levels, including GABA B R and GABA A R α1, α2, α3 and α5 subunits, measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. ( b ) Quantification and representative western blots of glutamatergic receptor subunits of NMDA receptor (GluN2A, GluN2B) and AMPA receptor (GluA1, GluA2) measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. Protein amount was normalized to β-tubulin and expressed as percentage (Values are means ± SEM; *P ≤ 0.05, two-tailed unpaired t-test with Welch’s correction).

    Techniques Used: Western Blot, Two Tailed Test

    rabbit anti gaba a r α5 polyclonal antibody  (Alomone Labs)


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    Alomone Labs rabbit anti gaba a r α5 polyclonal antibody
    ( A, B ) Relative mRNA expression (Mean + SEM, 2 -ΔCt ) of GABA-A R subunits in unchallenged ( A ) mBMDCs (n = 7 independent experiments) and ( B ) hMoDCs (n = 3 independent experiments). ( C, D ) Heat maps show (%) transcriptional changes in the expression of GABA-A R subunits upon challenge of ( C ) mBMDCs and ( D ) hMoDCs with T. gondii (PRU-RFP) relative to unchallenged cells at indicated time-point (n = 3–4 independent experiments). ( E ) Representative micrographs of unchallenged mBMDCs stained with antibodies against GABA-A R α3, <t>α5,</t> β3 and ρ1 subunit (and Alexa Flour 488-conjugated secondary antibodies), respectively, and Alexa Fluor 647 Phalloidin (F-actin) and DAPI (nuclei). Scale bars, 10 μm, for β3 5 μm (n = 3 independent experiments).
    Rabbit Anti Gaba A R α5 Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gaba a r α5 polyclonal antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gaba a r α5 polyclonal antibody - by Bioz Stars, 2023-01
    93/100 stars

    Images

    1) Product Images from "A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites"

    Article Title: A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites

    Journal: eLife

    doi: 10.7554/eLife.60528

    ( A, B ) Relative mRNA expression (Mean + SEM, 2 -ΔCt ) of GABA-A R subunits in unchallenged ( A ) mBMDCs (n = 7 independent experiments) and ( B ) hMoDCs (n = 3 independent experiments). ( C, D ) Heat maps show (%) transcriptional changes in the expression of GABA-A R subunits upon challenge of ( C ) mBMDCs and ( D ) hMoDCs with T. gondii (PRU-RFP) relative to unchallenged cells at indicated time-point (n = 3–4 independent experiments). ( E ) Representative micrographs of unchallenged mBMDCs stained with antibodies against GABA-A R α3, α5, β3 and ρ1 subunit (and Alexa Flour 488-conjugated secondary antibodies), respectively, and Alexa Fluor 647 Phalloidin (F-actin) and DAPI (nuclei). Scale bars, 10 μm, for β3 5 μm (n = 3 independent experiments).
    Figure Legend Snippet: ( A, B ) Relative mRNA expression (Mean + SEM, 2 -ΔCt ) of GABA-A R subunits in unchallenged ( A ) mBMDCs (n = 7 independent experiments) and ( B ) hMoDCs (n = 3 independent experiments). ( C, D ) Heat maps show (%) transcriptional changes in the expression of GABA-A R subunits upon challenge of ( C ) mBMDCs and ( D ) hMoDCs with T. gondii (PRU-RFP) relative to unchallenged cells at indicated time-point (n = 3–4 independent experiments). ( E ) Representative micrographs of unchallenged mBMDCs stained with antibodies against GABA-A R α3, α5, β3 and ρ1 subunit (and Alexa Flour 488-conjugated secondary antibodies), respectively, and Alexa Fluor 647 Phalloidin (F-actin) and DAPI (nuclei). Scale bars, 10 μm, for β3 5 μm (n = 3 independent experiments).

    Techniques Used: Expressing, Staining

    ( A, B ) Representative motility plots of unchallenged mBMDCs and T. gondii (PRU-RFP)-infected ( A ) mBMDCs and ( B ) hMoDCs treated with GABA-A R inhibitor picrotoxin (open channel blocker) or subunit specific inhibitors L655,708 (α-specific), SCS (β-specific) and TPMPA (ρ-specific) at concentrations stated in Materials and methods. X- and y-axes indicate distances in μm. ( C, D ) Box-and-whisker dot plots show, for each condition, median velocities (μm/min) as indicated in ( A ) and ( B ) (n = 3–6 independent experiments). ( E ) Representative motility plots and ( F ) velocities of unchallenged mBMDCs and T. gondii -infected mBMDCs treated with SNAP (GAT inhibitor) in presence of etomidate and allopregnanolone (allosteric modulators of β and δ subunit-containing GABA-A Rs, respectively). (n = 3–4 independent experiments). Statistical significance was tested by ordinary one-way ANOVA with Dunnett’s multiple comparison test for ( C, D, F ), ***p<0.001, ns p≥0.05.
    Figure Legend Snippet: ( A, B ) Representative motility plots of unchallenged mBMDCs and T. gondii (PRU-RFP)-infected ( A ) mBMDCs and ( B ) hMoDCs treated with GABA-A R inhibitor picrotoxin (open channel blocker) or subunit specific inhibitors L655,708 (α-specific), SCS (β-specific) and TPMPA (ρ-specific) at concentrations stated in Materials and methods. X- and y-axes indicate distances in μm. ( C, D ) Box-and-whisker dot plots show, for each condition, median velocities (μm/min) as indicated in ( A ) and ( B ) (n = 3–6 independent experiments). ( E ) Representative motility plots and ( F ) velocities of unchallenged mBMDCs and T. gondii -infected mBMDCs treated with SNAP (GAT inhibitor) in presence of etomidate and allopregnanolone (allosteric modulators of β and δ subunit-containing GABA-A Rs, respectively). (n = 3–4 independent experiments). Statistical significance was tested by ordinary one-way ANOVA with Dunnett’s multiple comparison test for ( C, D, F ), ***p<0.001, ns p≥0.05.

    Techniques Used: Infection, Whisker Assay

    ( A, B ) Box-and-whisker dot plots show median velocities (μm/min) of mBMDCs challenged with N. caninum Nc-1 strain ( A ) and Nc-Liverpool strain ( B ) and treated with GABA-A R inhibitors, NKCC1 inhibitor or VDCC inhibitors as indicated, respectively (n = 3 independent experiments). ( C ) Box-and-whisker dot plots show median velocities (μm/min) of hMonocytes challenged with T. gondii (PRU) and treated with GABA-A R inhibitor, NKCC1 inhibitor or VDCC inhibitor, respectively (n = 3 independent experiments). Statistical significance was assessed by ANOVA with Dunnett’s multiple comparison test for ( A, B, C ), ***p<0.001.
    Figure Legend Snippet: ( A, B ) Box-and-whisker dot plots show median velocities (μm/min) of mBMDCs challenged with N. caninum Nc-1 strain ( A ) and Nc-Liverpool strain ( B ) and treated with GABA-A R inhibitors, NKCC1 inhibitor or VDCC inhibitors as indicated, respectively (n = 3 independent experiments). ( C ) Box-and-whisker dot plots show median velocities (μm/min) of hMonocytes challenged with T. gondii (PRU) and treated with GABA-A R inhibitor, NKCC1 inhibitor or VDCC inhibitor, respectively (n = 3 independent experiments). Statistical significance was assessed by ANOVA with Dunnett’s multiple comparison test for ( A, B, C ), ***p<0.001.

    Techniques Used: Whisker Assay

    ( A, B ) The mRNA expression in unchallenged ( A ) mBMDCs and ( B ) hMoDCs, treated with shRNA for control Luc and GABA-A R subunits, related to mock-treated cells (Mean + SEM, %). For shα3, two separate constructs were used (n = 3–5 independent experiments for mBMDCs and 6–7 for hMoDCs). ( C, E ) Representative motility plots of ( C ) mBMDCs and ( E ) hMoDCs, treated as in ( A–B ) and challenged with T. gondii (PRU-RFP). X- and y-axes indicate distances in μm. ( D, F ) Histograms of accumulated distances migrated (μm) by ( D ) mBMDCs and ( F ) hMoDCs as in ( C and E ), respectively. Dotted lines indicate median values (n = 3–4 independent experiments). ( G ) Box-and-whisker dot plots show, for each indicated condition, median velocities (μm/min) of unchallenged and T. gondii -infected mBMDCs and hMoDCs (n = 3–4 independent experiments). Statistical significance was tested by ordinary one-way ANOVA with Dunnett’s multiple comparison test for ( A, B, G ) and by Mann-Whitney test for ( D, F ), *p<0.05, **p<0.01, ***p<0.001, ns p≥0.05.
    Figure Legend Snippet: ( A, B ) The mRNA expression in unchallenged ( A ) mBMDCs and ( B ) hMoDCs, treated with shRNA for control Luc and GABA-A R subunits, related to mock-treated cells (Mean + SEM, %). For shα3, two separate constructs were used (n = 3–5 independent experiments for mBMDCs and 6–7 for hMoDCs). ( C, E ) Representative motility plots of ( C ) mBMDCs and ( E ) hMoDCs, treated as in ( A–B ) and challenged with T. gondii (PRU-RFP). X- and y-axes indicate distances in μm. ( D, F ) Histograms of accumulated distances migrated (μm) by ( D ) mBMDCs and ( F ) hMoDCs as in ( C and E ), respectively. Dotted lines indicate median values (n = 3–4 independent experiments). ( G ) Box-and-whisker dot plots show, for each indicated condition, median velocities (μm/min) of unchallenged and T. gondii -infected mBMDCs and hMoDCs (n = 3–4 independent experiments). Statistical significance was tested by ordinary one-way ANOVA with Dunnett’s multiple comparison test for ( A, B, G ) and by Mann-Whitney test for ( D, F ), *p<0.05, **p<0.01, ***p<0.001, ns p≥0.05.

    Techniques Used: Expressing, shRNA, Construct, Whisker Assay, Infection, MANN-WHITNEY

    ( A ) Schematic illustration of simultaneous adoptive transfers of T. gondii (ME49/PTG-GFP)-challenged mBMDCs prelabeled with CMF2HC or CMTMR dye and pretreated as described in Materials and methods, respectively. ( B, C ) Representative bivariate plots show CD11c + cells from ( B ) peritoneal cavity and ( C ) spleen of C57BL/6 mice inoculated with T. gondii -challenged mBMDCs prelabeled with CMF2HC or CMTMR dye. Cells were analyzed by flow cytometry at 14–18 h post-inoculation (gating strategy in  ). Plots in upper, center and lower rows show, respectively, non-treated cells, cells pretreated with GABA-A R inhibitors (GABA-A R i) and NKCC1 inhibitor (NKCC1 i). ( D ) Bar graph shows the ratio of treated cells (CD11c + GFP + CMTMR + ) to non-treated cells (CD11c + GFP + CMF2HC + ) in peritoneal lavage and spleen after adoptive transfer of T. gondii -challenged mBMDCs (n = 7–10 mice per group). ( E ) Parasite loads in spleen and liver of C57BL/6 mice at day 4 post-inoculation of pharmacologically treated cells (GABA-A R i, NKCC1 i) related to non-treated cells and measured by plaquing assays (n = 5–6 mice per group). ( F ) Parasite loads in spleen and liver of C57BL/6 mice at day 5 post-inoculation of gene-silenced cells (shρ1, shNKCC1) related to mock- and control shLuc-transduced cells and measured by plaquing assays (n = 6 mice per group). ( G ) Parasite loads in spleen and brain of CD1 mice at day 7 post-inoculation of pharmacologically treated cells (GABA-A R i, NKCC1 i) related to non-treated cells and measured by plaquing assays (n = 9–10 mice per group). Bar graphs show mean + SEM. Statistical significance was assessed by ordinary one-way ANOVA with Tukey’s multiple comparison test, *p<0.05, **p<0.01, ***p<0.001, ns p≥0.05.
    Figure Legend Snippet: ( A ) Schematic illustration of simultaneous adoptive transfers of T. gondii (ME49/PTG-GFP)-challenged mBMDCs prelabeled with CMF2HC or CMTMR dye and pretreated as described in Materials and methods, respectively. ( B, C ) Representative bivariate plots show CD11c + cells from ( B ) peritoneal cavity and ( C ) spleen of C57BL/6 mice inoculated with T. gondii -challenged mBMDCs prelabeled with CMF2HC or CMTMR dye. Cells were analyzed by flow cytometry at 14–18 h post-inoculation (gating strategy in ). Plots in upper, center and lower rows show, respectively, non-treated cells, cells pretreated with GABA-A R inhibitors (GABA-A R i) and NKCC1 inhibitor (NKCC1 i). ( D ) Bar graph shows the ratio of treated cells (CD11c + GFP + CMTMR + ) to non-treated cells (CD11c + GFP + CMF2HC + ) in peritoneal lavage and spleen after adoptive transfer of T. gondii -challenged mBMDCs (n = 7–10 mice per group). ( E ) Parasite loads in spleen and liver of C57BL/6 mice at day 4 post-inoculation of pharmacologically treated cells (GABA-A R i, NKCC1 i) related to non-treated cells and measured by plaquing assays (n = 5–6 mice per group). ( F ) Parasite loads in spleen and liver of C57BL/6 mice at day 5 post-inoculation of gene-silenced cells (shρ1, shNKCC1) related to mock- and control shLuc-transduced cells and measured by plaquing assays (n = 6 mice per group). ( G ) Parasite loads in spleen and brain of CD1 mice at day 7 post-inoculation of pharmacologically treated cells (GABA-A R i, NKCC1 i) related to non-treated cells and measured by plaquing assays (n = 9–10 mice per group). Bar graphs show mean + SEM. Statistical significance was assessed by ordinary one-way ANOVA with Tukey’s multiple comparison test, *p<0.05, **p<0.01, ***p<0.001, ns p≥0.05.

    Techniques Used: Flow Cytometry, Adoptive Transfer Assay

    rabbit anti α5 gaba a r  (Alomone Labs)


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    Alomone Labs rabbit anti α5 gaba a r
    (a) Southern blot screen of ES cell lines: the Q241M mutation silently introduces a novel restriction site – allowing a screen for restriction fragment length polymorphism. Lanes 1 - 5, heterozygous recombinants; lanes 6 - 7, wild-type littermate controls. (b) Verifying the point mutation by DNA sequencing. Exon 8 was PCR-amplified from mouse genomic DNA of wild-type (α2 Q/Q : left), α2 Q/M (middle) and α2 M/M (right) animals, then sequenced. Sequence data around the point mutation are shown. (c,d) <t>GABA</t> <t>A</t> <t>R</t> α1-α4 subunit expression levels were determined by Western blotting total protein isolated from the cortex ( c ) and hippocampus ( d ). Left panels, quantitation of expression in α2 Q/M (grey) and α2 M/M (white) relative to wild-type (α2 Q/Q , black) brain samples. Right panels, representative Western blots from cortex and hippocampus for wild-type (WT), α2 Q/M and α2 M/M mice. Images show blots between 45 and 66 kDa markers. Loading controls with tubulin are omitted for clarity. Data are mean ± sem, one-way ANOVA, n = 6-9. There are no statistically significant differences between groups.
    Rabbit Anti α5 Gaba A R, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti α5 gaba a r/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti α5 gaba a r - by Bioz Stars, 2023-01
    93/100 stars

    Images

    1) Product Images from "Brain neurosteroids are natural anxiolytics targeting α2 subunit γ-aminobutyric acid type-A receptors"

    Article Title: Brain neurosteroids are natural anxiolytics targeting α2 subunit γ-aminobutyric acid type-A receptors

    Journal: bioRxiv

    doi: 10.1101/462457

    (a) Southern blot screen of ES cell lines: the Q241M mutation silently introduces a novel restriction site – allowing a screen for restriction fragment length polymorphism. Lanes 1 - 5, heterozygous recombinants; lanes 6 - 7, wild-type littermate controls. (b) Verifying the point mutation by DNA sequencing. Exon 8 was PCR-amplified from mouse genomic DNA of wild-type (α2 Q/Q : left), α2 Q/M (middle) and α2 M/M (right) animals, then sequenced. Sequence data around the point mutation are shown. (c,d) GABA A R α1-α4 subunit expression levels were determined by Western blotting total protein isolated from the cortex ( c ) and hippocampus ( d ). Left panels, quantitation of expression in α2 Q/M (grey) and α2 M/M (white) relative to wild-type (α2 Q/Q , black) brain samples. Right panels, representative Western blots from cortex and hippocampus for wild-type (WT), α2 Q/M and α2 M/M mice. Images show blots between 45 and 66 kDa markers. Loading controls with tubulin are omitted for clarity. Data are mean ± sem, one-way ANOVA, n = 6-9. There are no statistically significant differences between groups.
    Figure Legend Snippet: (a) Southern blot screen of ES cell lines: the Q241M mutation silently introduces a novel restriction site – allowing a screen for restriction fragment length polymorphism. Lanes 1 - 5, heterozygous recombinants; lanes 6 - 7, wild-type littermate controls. (b) Verifying the point mutation by DNA sequencing. Exon 8 was PCR-amplified from mouse genomic DNA of wild-type (α2 Q/Q : left), α2 Q/M (middle) and α2 M/M (right) animals, then sequenced. Sequence data around the point mutation are shown. (c,d) GABA A R α1-α4 subunit expression levels were determined by Western blotting total protein isolated from the cortex ( c ) and hippocampus ( d ). Left panels, quantitation of expression in α2 Q/M (grey) and α2 M/M (white) relative to wild-type (α2 Q/Q , black) brain samples. Right panels, representative Western blots from cortex and hippocampus for wild-type (WT), α2 Q/M and α2 M/M mice. Images show blots between 45 and 66 kDa markers. Loading controls with tubulin are omitted for clarity. Data are mean ± sem, one-way ANOVA, n = 6-9. There are no statistically significant differences between groups.

    Techniques Used: Southern Blot, Mutagenesis, DNA Sequencing, Amplification, Sequencing, Expressing, Western Blot, Isolation, Quantitation Assay

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    Alomone Labs anti gaba a r α5
    Protein levels of the main GABAergic and glutamatergic receptors in 6 month old male hAPP wt mice. ( a ) Quantification and representative western blots of GABAergic receptor protein levels, including GABA B R and <t>GABA</t> <t>A</t> <t>R</t> α1, α2, α3 and <t>α5</t> subunits, measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. ( b ) Quantification and representative western blots of glutamatergic receptor subunits of NMDA receptor (GluN2A, GluN2B) and AMPA receptor (GluA1, GluA2) measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. Protein amount was normalized to β-tubulin and expressed as percentage (Values are means ± SEM; *P ≤ 0.05, two-tailed unpaired t-test with Welch’s correction).
    Anti Gaba A R α5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gaba a r α5/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gaba a r α5 - by Bioz Stars, 2023-01
    93/100 stars
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    Alomone Labs rabbit anti gaba a r α5 polyclonal antibody
    ( A, B ) Relative mRNA expression (Mean + SEM, 2 -ΔCt ) of GABA-A R subunits in unchallenged ( A ) mBMDCs (n = 7 independent experiments) and ( B ) hMoDCs (n = 3 independent experiments). ( C, D ) Heat maps show (%) transcriptional changes in the expression of GABA-A R subunits upon challenge of ( C ) mBMDCs and ( D ) hMoDCs with T. gondii (PRU-RFP) relative to unchallenged cells at indicated time-point (n = 3–4 independent experiments). ( E ) Representative micrographs of unchallenged mBMDCs stained with antibodies against GABA-A R α3, <t>α5,</t> β3 and ρ1 subunit (and Alexa Flour 488-conjugated secondary antibodies), respectively, and Alexa Fluor 647 Phalloidin (F-actin) and DAPI (nuclei). Scale bars, 10 μm, for β3 5 μm (n = 3 independent experiments).
    Rabbit Anti Gaba A R α5 Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gaba a r α5 polyclonal antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gaba a r α5 polyclonal antibody - by Bioz Stars, 2023-01
    93/100 stars
      Buy from Supplier

    93
    Alomone Labs rabbit anti α5 gaba a r
    (a) Southern blot screen of ES cell lines: the Q241M mutation silently introduces a novel restriction site – allowing a screen for restriction fragment length polymorphism. Lanes 1 - 5, heterozygous recombinants; lanes 6 - 7, wild-type littermate controls. (b) Verifying the point mutation by DNA sequencing. Exon 8 was PCR-amplified from mouse genomic DNA of wild-type (α2 Q/Q : left), α2 Q/M (middle) and α2 M/M (right) animals, then sequenced. Sequence data around the point mutation are shown. (c,d) <t>GABA</t> <t>A</t> <t>R</t> α1-α4 subunit expression levels were determined by Western blotting total protein isolated from the cortex ( c ) and hippocampus ( d ). Left panels, quantitation of expression in α2 Q/M (grey) and α2 M/M (white) relative to wild-type (α2 Q/Q , black) brain samples. Right panels, representative Western blots from cortex and hippocampus for wild-type (WT), α2 Q/M and α2 M/M mice. Images show blots between 45 and 66 kDa markers. Loading controls with tubulin are omitted for clarity. Data are mean ± sem, one-way ANOVA, n = 6-9. There are no statistically significant differences between groups.
    Rabbit Anti α5 Gaba A R, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti α5 gaba a r/product/Alomone Labs
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    Protein levels of the main GABAergic and glutamatergic receptors in 6 month old male hAPP wt mice. ( a ) Quantification and representative western blots of GABAergic receptor protein levels, including GABA B R and GABA A R α1, α2, α3 and α5 subunits, measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. ( b ) Quantification and representative western blots of glutamatergic receptor subunits of NMDA receptor (GluN2A, GluN2B) and AMPA receptor (GluA1, GluA2) measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. Protein amount was normalized to β-tubulin and expressed as percentage (Values are means ± SEM; *P ≤ 0.05, two-tailed unpaired t-test with Welch’s correction).

    Journal: Scientific Reports

    Article Title: Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission

    doi: 10.1038/s41598-021-97144-3

    Figure Lengend Snippet: Protein levels of the main GABAergic and glutamatergic receptors in 6 month old male hAPP wt mice. ( a ) Quantification and representative western blots of GABAergic receptor protein levels, including GABA B R and GABA A R α1, α2, α3 and α5 subunits, measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. ( b ) Quantification and representative western blots of glutamatergic receptor subunits of NMDA receptor (GluN2A, GluN2B) and AMPA receptor (GluA1, GluA2) measured in hippocampal tissue lysates of hAPP wt (n = 4) and control (n = 4) animals. Protein amount was normalized to β-tubulin and expressed as percentage (Values are means ± SEM; *P ≤ 0.05, two-tailed unpaired t-test with Welch’s correction).

    Article Snippet: Membranes were incubated in appropriate primary antibodies (anti-KCC2 1:3000 (Merck), anti-NKCC1 1:1000 (Abcam), anti-β-tubulin 1:10,000 (Neuromics), anti-human APP W02 1:2000 (Merk), total APP anti-C-terminus 1:4000 (Sigma Aldrich), anti-soluble APP clone 22C11 1:500 (Merck), anti-GABA B R 1:500 (Sigma Aldrich), anti-GABA A R α1 1:1000 (Alomone labs), anti-GABA A R α2 1:1000 (Abcam), anti-GABA A R α3 1:1000 (Alomone labs), anti-GABA A R α5 1:1000 (Alomone labs), anti-GluA1 1:500 (Merck), anti-GluA2 1:1000 (Merck), anti-GluN2A 1:250 (Merck), anti-GluN2B 1:500 (BD Biosciences), anti-GAD65/67 1:1000 (Abcam)) at 4 °C, overnight.

    Techniques: Western Blot, Two Tailed Test

    ( A, B ) Relative mRNA expression (Mean + SEM, 2 -ΔCt ) of GABA-A R subunits in unchallenged ( A ) mBMDCs (n = 7 independent experiments) and ( B ) hMoDCs (n = 3 independent experiments). ( C, D ) Heat maps show (%) transcriptional changes in the expression of GABA-A R subunits upon challenge of ( C ) mBMDCs and ( D ) hMoDCs with T. gondii (PRU-RFP) relative to unchallenged cells at indicated time-point (n = 3–4 independent experiments). ( E ) Representative micrographs of unchallenged mBMDCs stained with antibodies against GABA-A R α3, α5, β3 and ρ1 subunit (and Alexa Flour 488-conjugated secondary antibodies), respectively, and Alexa Fluor 647 Phalloidin (F-actin) and DAPI (nuclei). Scale bars, 10 μm, for β3 5 μm (n = 3 independent experiments).

    Journal: eLife

    Article Title: A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites

    doi: 10.7554/eLife.60528

    Figure Lengend Snippet: ( A, B ) Relative mRNA expression (Mean + SEM, 2 -ΔCt ) of GABA-A R subunits in unchallenged ( A ) mBMDCs (n = 7 independent experiments) and ( B ) hMoDCs (n = 3 independent experiments). ( C, D ) Heat maps show (%) transcriptional changes in the expression of GABA-A R subunits upon challenge of ( C ) mBMDCs and ( D ) hMoDCs with T. gondii (PRU-RFP) relative to unchallenged cells at indicated time-point (n = 3–4 independent experiments). ( E ) Representative micrographs of unchallenged mBMDCs stained with antibodies against GABA-A R α3, α5, β3 and ρ1 subunit (and Alexa Flour 488-conjugated secondary antibodies), respectively, and Alexa Fluor 647 Phalloidin (F-actin) and DAPI (nuclei). Scale bars, 10 μm, for β3 5 μm (n = 3 independent experiments).

    Article Snippet: To probe GABA-A R subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody, rabbit anti-GABA-A R α5 polyclonal antibody, rabbit anti-GABA-A R ρ1 polyclonal antibody (all from Alomone labs, Jerusalem, Israel), mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab, UC Davis, CA, US) and for NKCC, with mouse anti-NKCC1/2 monoclonal antibody (clone T4, Developmental Studies Hybridoma Bank, DSHB, Iowa, USA) ON at 4°C.

    Techniques: Expressing, Staining

    ( A, B ) Representative motility plots of unchallenged mBMDCs and T. gondii (PRU-RFP)-infected ( A ) mBMDCs and ( B ) hMoDCs treated with GABA-A R inhibitor picrotoxin (open channel blocker) or subunit specific inhibitors L655,708 (α-specific), SCS (β-specific) and TPMPA (ρ-specific) at concentrations stated in Materials and methods. X- and y-axes indicate distances in μm. ( C, D ) Box-and-whisker dot plots show, for each condition, median velocities (μm/min) as indicated in ( A ) and ( B ) (n = 3–6 independent experiments). ( E ) Representative motility plots and ( F ) velocities of unchallenged mBMDCs and T. gondii -infected mBMDCs treated with SNAP (GAT inhibitor) in presence of etomidate and allopregnanolone (allosteric modulators of β and δ subunit-containing GABA-A Rs, respectively). (n = 3–4 independent experiments). Statistical significance was tested by ordinary one-way ANOVA with Dunnett’s multiple comparison test for ( C, D, F ), ***p<0.001, ns p≥0.05.

    Journal: eLife

    Article Title: A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites

    doi: 10.7554/eLife.60528

    Figure Lengend Snippet: ( A, B ) Representative motility plots of unchallenged mBMDCs and T. gondii (PRU-RFP)-infected ( A ) mBMDCs and ( B ) hMoDCs treated with GABA-A R inhibitor picrotoxin (open channel blocker) or subunit specific inhibitors L655,708 (α-specific), SCS (β-specific) and TPMPA (ρ-specific) at concentrations stated in Materials and methods. X- and y-axes indicate distances in μm. ( C, D ) Box-and-whisker dot plots show, for each condition, median velocities (μm/min) as indicated in ( A ) and ( B ) (n = 3–6 independent experiments). ( E ) Representative motility plots and ( F ) velocities of unchallenged mBMDCs and T. gondii -infected mBMDCs treated with SNAP (GAT inhibitor) in presence of etomidate and allopregnanolone (allosteric modulators of β and δ subunit-containing GABA-A Rs, respectively). (n = 3–4 independent experiments). Statistical significance was tested by ordinary one-way ANOVA with Dunnett’s multiple comparison test for ( C, D, F ), ***p<0.001, ns p≥0.05.

    Article Snippet: To probe GABA-A R subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody, rabbit anti-GABA-A R α5 polyclonal antibody, rabbit anti-GABA-A R ρ1 polyclonal antibody (all from Alomone labs, Jerusalem, Israel), mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab, UC Davis, CA, US) and for NKCC, with mouse anti-NKCC1/2 monoclonal antibody (clone T4, Developmental Studies Hybridoma Bank, DSHB, Iowa, USA) ON at 4°C.

    Techniques: Infection, Whisker Assay

    ( A, B ) Box-and-whisker dot plots show median velocities (μm/min) of mBMDCs challenged with N. caninum Nc-1 strain ( A ) and Nc-Liverpool strain ( B ) and treated with GABA-A R inhibitors, NKCC1 inhibitor or VDCC inhibitors as indicated, respectively (n = 3 independent experiments). ( C ) Box-and-whisker dot plots show median velocities (μm/min) of hMonocytes challenged with T. gondii (PRU) and treated with GABA-A R inhibitor, NKCC1 inhibitor or VDCC inhibitor, respectively (n = 3 independent experiments). Statistical significance was assessed by ANOVA with Dunnett’s multiple comparison test for ( A, B, C ), ***p<0.001.

    Journal: eLife

    Article Title: A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites

    doi: 10.7554/eLife.60528

    Figure Lengend Snippet: ( A, B ) Box-and-whisker dot plots show median velocities (μm/min) of mBMDCs challenged with N. caninum Nc-1 strain ( A ) and Nc-Liverpool strain ( B ) and treated with GABA-A R inhibitors, NKCC1 inhibitor or VDCC inhibitors as indicated, respectively (n = 3 independent experiments). ( C ) Box-and-whisker dot plots show median velocities (μm/min) of hMonocytes challenged with T. gondii (PRU) and treated with GABA-A R inhibitor, NKCC1 inhibitor or VDCC inhibitor, respectively (n = 3 independent experiments). Statistical significance was assessed by ANOVA with Dunnett’s multiple comparison test for ( A, B, C ), ***p<0.001.

    Article Snippet: To probe GABA-A R subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody, rabbit anti-GABA-A R α5 polyclonal antibody, rabbit anti-GABA-A R ρ1 polyclonal antibody (all from Alomone labs, Jerusalem, Israel), mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab, UC Davis, CA, US) and for NKCC, with mouse anti-NKCC1/2 monoclonal antibody (clone T4, Developmental Studies Hybridoma Bank, DSHB, Iowa, USA) ON at 4°C.

    Techniques: Whisker Assay

    ( A, B ) The mRNA expression in unchallenged ( A ) mBMDCs and ( B ) hMoDCs, treated with shRNA for control Luc and GABA-A R subunits, related to mock-treated cells (Mean + SEM, %). For shα3, two separate constructs were used (n = 3–5 independent experiments for mBMDCs and 6–7 for hMoDCs). ( C, E ) Representative motility plots of ( C ) mBMDCs and ( E ) hMoDCs, treated as in ( A–B ) and challenged with T. gondii (PRU-RFP). X- and y-axes indicate distances in μm. ( D, F ) Histograms of accumulated distances migrated (μm) by ( D ) mBMDCs and ( F ) hMoDCs as in ( C and E ), respectively. Dotted lines indicate median values (n = 3–4 independent experiments). ( G ) Box-and-whisker dot plots show, for each indicated condition, median velocities (μm/min) of unchallenged and T. gondii -infected mBMDCs and hMoDCs (n = 3–4 independent experiments). Statistical significance was tested by ordinary one-way ANOVA with Dunnett’s multiple comparison test for ( A, B, G ) and by Mann-Whitney test for ( D, F ), *p<0.05, **p<0.01, ***p<0.001, ns p≥0.05.

    Journal: eLife

    Article Title: A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites

    doi: 10.7554/eLife.60528

    Figure Lengend Snippet: ( A, B ) The mRNA expression in unchallenged ( A ) mBMDCs and ( B ) hMoDCs, treated with shRNA for control Luc and GABA-A R subunits, related to mock-treated cells (Mean + SEM, %). For shα3, two separate constructs were used (n = 3–5 independent experiments for mBMDCs and 6–7 for hMoDCs). ( C, E ) Representative motility plots of ( C ) mBMDCs and ( E ) hMoDCs, treated as in ( A–B ) and challenged with T. gondii (PRU-RFP). X- and y-axes indicate distances in μm. ( D, F ) Histograms of accumulated distances migrated (μm) by ( D ) mBMDCs and ( F ) hMoDCs as in ( C and E ), respectively. Dotted lines indicate median values (n = 3–4 independent experiments). ( G ) Box-and-whisker dot plots show, for each indicated condition, median velocities (μm/min) of unchallenged and T. gondii -infected mBMDCs and hMoDCs (n = 3–4 independent experiments). Statistical significance was tested by ordinary one-way ANOVA with Dunnett’s multiple comparison test for ( A, B, G ) and by Mann-Whitney test for ( D, F ), *p<0.05, **p<0.01, ***p<0.001, ns p≥0.05.

    Article Snippet: To probe GABA-A R subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody, rabbit anti-GABA-A R α5 polyclonal antibody, rabbit anti-GABA-A R ρ1 polyclonal antibody (all from Alomone labs, Jerusalem, Israel), mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab, UC Davis, CA, US) and for NKCC, with mouse anti-NKCC1/2 monoclonal antibody (clone T4, Developmental Studies Hybridoma Bank, DSHB, Iowa, USA) ON at 4°C.

    Techniques: Expressing, shRNA, Construct, Whisker Assay, Infection, MANN-WHITNEY

    ( A ) Schematic illustration of simultaneous adoptive transfers of T. gondii (ME49/PTG-GFP)-challenged mBMDCs prelabeled with CMF2HC or CMTMR dye and pretreated as described in Materials and methods, respectively. ( B, C ) Representative bivariate plots show CD11c + cells from ( B ) peritoneal cavity and ( C ) spleen of C57BL/6 mice inoculated with T. gondii -challenged mBMDCs prelabeled with CMF2HC or CMTMR dye. Cells were analyzed by flow cytometry at 14–18 h post-inoculation (gating strategy in  ). Plots in upper, center and lower rows show, respectively, non-treated cells, cells pretreated with GABA-A R inhibitors (GABA-A R i) and NKCC1 inhibitor (NKCC1 i). ( D ) Bar graph shows the ratio of treated cells (CD11c + GFP + CMTMR + ) to non-treated cells (CD11c + GFP + CMF2HC + ) in peritoneal lavage and spleen after adoptive transfer of T. gondii -challenged mBMDCs (n = 7–10 mice per group). ( E ) Parasite loads in spleen and liver of C57BL/6 mice at day 4 post-inoculation of pharmacologically treated cells (GABA-A R i, NKCC1 i) related to non-treated cells and measured by plaquing assays (n = 5–6 mice per group). ( F ) Parasite loads in spleen and liver of C57BL/6 mice at day 5 post-inoculation of gene-silenced cells (shρ1, shNKCC1) related to mock- and control shLuc-transduced cells and measured by plaquing assays (n = 6 mice per group). ( G ) Parasite loads in spleen and brain of CD1 mice at day 7 post-inoculation of pharmacologically treated cells (GABA-A R i, NKCC1 i) related to non-treated cells and measured by plaquing assays (n = 9–10 mice per group). Bar graphs show mean + SEM. Statistical significance was assessed by ordinary one-way ANOVA with Tukey’s multiple comparison test, *p<0.05, **p<0.01, ***p<0.001, ns p≥0.05.

    Journal: eLife

    Article Title: A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites

    doi: 10.7554/eLife.60528

    Figure Lengend Snippet: ( A ) Schematic illustration of simultaneous adoptive transfers of T. gondii (ME49/PTG-GFP)-challenged mBMDCs prelabeled with CMF2HC or CMTMR dye and pretreated as described in Materials and methods, respectively. ( B, C ) Representative bivariate plots show CD11c + cells from ( B ) peritoneal cavity and ( C ) spleen of C57BL/6 mice inoculated with T. gondii -challenged mBMDCs prelabeled with CMF2HC or CMTMR dye. Cells were analyzed by flow cytometry at 14–18 h post-inoculation (gating strategy in ). Plots in upper, center and lower rows show, respectively, non-treated cells, cells pretreated with GABA-A R inhibitors (GABA-A R i) and NKCC1 inhibitor (NKCC1 i). ( D ) Bar graph shows the ratio of treated cells (CD11c + GFP + CMTMR + ) to non-treated cells (CD11c + GFP + CMF2HC + ) in peritoneal lavage and spleen after adoptive transfer of T. gondii -challenged mBMDCs (n = 7–10 mice per group). ( E ) Parasite loads in spleen and liver of C57BL/6 mice at day 4 post-inoculation of pharmacologically treated cells (GABA-A R i, NKCC1 i) related to non-treated cells and measured by plaquing assays (n = 5–6 mice per group). ( F ) Parasite loads in spleen and liver of C57BL/6 mice at day 5 post-inoculation of gene-silenced cells (shρ1, shNKCC1) related to mock- and control shLuc-transduced cells and measured by plaquing assays (n = 6 mice per group). ( G ) Parasite loads in spleen and brain of CD1 mice at day 7 post-inoculation of pharmacologically treated cells (GABA-A R i, NKCC1 i) related to non-treated cells and measured by plaquing assays (n = 9–10 mice per group). Bar graphs show mean + SEM. Statistical significance was assessed by ordinary one-way ANOVA with Tukey’s multiple comparison test, *p<0.05, **p<0.01, ***p<0.001, ns p≥0.05.

    Article Snippet: To probe GABA-A R subunits, cells were incubated with rabbit anti-GABA-A R α3 polyclonal antibody, rabbit anti-GABA-A R α5 polyclonal antibody, rabbit anti-GABA-A R ρ1 polyclonal antibody (all from Alomone labs, Jerusalem, Israel), mouse anti-GABA-A R β3 monoclonal antibody (NeuroMab, UC Davis, CA, US) and for NKCC, with mouse anti-NKCC1/2 monoclonal antibody (clone T4, Developmental Studies Hybridoma Bank, DSHB, Iowa, USA) ON at 4°C.

    Techniques: Flow Cytometry, Adoptive Transfer Assay

    (a) Southern blot screen of ES cell lines: the Q241M mutation silently introduces a novel restriction site – allowing a screen for restriction fragment length polymorphism. Lanes 1 - 5, heterozygous recombinants; lanes 6 - 7, wild-type littermate controls. (b) Verifying the point mutation by DNA sequencing. Exon 8 was PCR-amplified from mouse genomic DNA of wild-type (α2 Q/Q : left), α2 Q/M (middle) and α2 M/M (right) animals, then sequenced. Sequence data around the point mutation are shown. (c,d) GABA A R α1-α4 subunit expression levels were determined by Western blotting total protein isolated from the cortex ( c ) and hippocampus ( d ). Left panels, quantitation of expression in α2 Q/M (grey) and α2 M/M (white) relative to wild-type (α2 Q/Q , black) brain samples. Right panels, representative Western blots from cortex and hippocampus for wild-type (WT), α2 Q/M and α2 M/M mice. Images show blots between 45 and 66 kDa markers. Loading controls with tubulin are omitted for clarity. Data are mean ± sem, one-way ANOVA, n = 6-9. There are no statistically significant differences between groups.

    Journal: bioRxiv

    Article Title: Brain neurosteroids are natural anxiolytics targeting α2 subunit γ-aminobutyric acid type-A receptors

    doi: 10.1101/462457

    Figure Lengend Snippet: (a) Southern blot screen of ES cell lines: the Q241M mutation silently introduces a novel restriction site – allowing a screen for restriction fragment length polymorphism. Lanes 1 - 5, heterozygous recombinants; lanes 6 - 7, wild-type littermate controls. (b) Verifying the point mutation by DNA sequencing. Exon 8 was PCR-amplified from mouse genomic DNA of wild-type (α2 Q/Q : left), α2 Q/M (middle) and α2 M/M (right) animals, then sequenced. Sequence data around the point mutation are shown. (c,d) GABA A R α1-α4 subunit expression levels were determined by Western blotting total protein isolated from the cortex ( c ) and hippocampus ( d ). Left panels, quantitation of expression in α2 Q/M (grey) and α2 M/M (white) relative to wild-type (α2 Q/Q , black) brain samples. Right panels, representative Western blots from cortex and hippocampus for wild-type (WT), α2 Q/M and α2 M/M mice. Images show blots between 45 and 66 kDa markers. Loading controls with tubulin are omitted for clarity. Data are mean ± sem, one-way ANOVA, n = 6-9. There are no statistically significant differences between groups.

    Article Snippet: For immunohistochemistry, the following primary antibodies were used: rabbit anti-α1-GABA A R (1:20000, from Jean-Marc Fritschy, University of Zurich), guinea-pig anti-α2-GABA A R (1:1000, from Jean-Marc Fritschy), rabbit anti-α3-GABA A R (1:1000, Alomone Labs), rabbit anti-α4-GABA A R (1:500, Werner Sieghart), rabbit anti-α5-GABA A R (1:500, Werner Sieghart).

    Techniques: Southern Blot, Mutagenesis, DNA Sequencing, Amplification, Sequencing, Expressing, Western Blot, Isolation, Quantitation Assay